Abstract：
:This study examined the expression of EPO, VEGF and VEGF receptor gene under conditions of reduced oxygen supply in primary cultures of rat hepatocytes, and compared it with the expression of these genes in hypoxic rat livers in vivo. To this end we exposed male Sprague-Dawley rats to hypoxia (10% and 8% O2), carbon monoxide (0.1% CO) or injected cobalt chloride (60 mg/kg CoCl2) subcutaneously. For the in vitro experiments we used primary cultures of rat hepatocytes which were kept at high (20% O2) and low (1% O2) oxygen tensions for three hours. The EPO mRNA was up-regulated by hypoxia in vitro and in vivo about 10-fold. The VEGF mRNA was up-regulated fivefold in the hepatocytes only, whereas the in vivo mRNA levels remained unchanged. The mRNA levels of flt-1 were up-regulated threefold by 8% O2 in livers, dependent on the strength of hypoxia (10% caused no changes in flt-1 gene expression) and on the kind of hypoxic stimulus (8% O2 was as effective as 0.1% CO and more effective than cobalt). The mRNA levels of flk-1/KDR and flt-4 remained unchanged in the liver. In vitro there were no changes in the mRNA levels of flt-1, flt-4 and flk-1/KDR. Consequently, the in vivo regulation of VEGF, which might be modulated by induction of flt-1 receptor gene expression, differs from the in vitro cell culture situation and might be different from the EPO regulation in vivo.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Sandner P,Wolf K,Bergmaier U,Gess B,Kurtz Adoi
10.1038/ki.1997.60subject
Has Abstractpub_date
1997-02-01 00:00:00pages
448-53issue
2eissn
0085-2538issn
1523-1755pii
S0085-2538(15)59900-6journal_volume
51pub_type
杂志文章abstract：:Fixed anionic sites within the glomerular capillary wall influence the permeation of serum proteins, the localization of various antigens, and the deposition of antibody in the subepithelial space. In anti-GBM nephritis antibody deposition occurs very rapidly to antigenic sites located relatively proximal in the glome...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1984.188
更新日期：1984-10-01 00:00:00
abstract：BACKGROUND:Lanthanum carbonate has been shown to be a safe, effective phosphate-binding agent. We have shown that an impaired mineralization in chronic renal failure rats treated with high doses of lanthanum carbonate develops secondary to phosphate depletion and is therefore pharmacologically mediated rather than a di...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00281.x
更新日期：2005-05-01 00:00:00
abstract：:How much dialysis is required for optimal well-being and long-term survival? The Frequent Hemodialysis Network completed two prospective trials examining the effect of more frequent, short hours and, now, of nocturnal hemodialysis compared with standard thrice-weekly treatments. Whereas the short-hours trial reported ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2011.267
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:Hemodialysis access thrombosis is a significant cause of morbidity for hemodialysis patients and results from decreased access flow caused by venous outflow tract stenosis. Ultrasound dilution (UD) is a practical, noninvasive, and reliable indicator of access flow and is effective in predicting venous stenos...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1046/j.1523-1755.2001.00753.x
更新日期：2001-06-01 00:00:00
abstract：:The impact of pediatric chronic kidney disease (CKD) on acquisition of volumetric bone mineral density (BMD) and cortical dimensions is lacking. To address this issue, we obtained tibia quantitative computed tomography scans from 103 patients aged 5-21 years with CKD (26 on dialysis) at baseline and 12 months later. G...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2012.347
更新日期：2013-03-01 00:00:00
abstract：:Renal fibrosis is a common pathway leading to the progression of chronic kidney disease, and bone marrow-derived fibroblasts contribute significantly to the development of renal fibrosis. However, the signaling mechanisms underlying the activation of these fibroblasts are not completely understood. Here, we examined t...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.04.021
更新日期：2017-12-01 00:00:00
abstract：:Nitric oxide is generated from L-arginine by nitric oxide synthase (NOS), an enzyme that exists in several isoforms. Some studies found that a polymorphism (G894T) in the endothelial NOS gene was associated with decreased nitric oxide bioactivity and vascular complications. However, it is not known whether the enzyme ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.574
更新日期：2009-02-01 00:00:00
abstract：:An open, randomized trial study on the therapeutic effect of recombinant alpha-interferon (IFN alpha) in 40 patients with hepatitis B virus membranous nephropathy (HBVMN) was conducted. All were pathologically proven to have HBVMN which showed no response to corticosteroid treatment represented by persistent heavy pro...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/ki.1995.27
更新日期：1995-01-01 00:00:00
abstract：:The hypertrophy of renal proximal tubular cells occurs as an adaptive response to a variety of stimuli and may be involved with the progression of renal disease. Angiotensin II acting alone or in combination with other growth factors has been implicated in this process. The aims of this study were to identify the role...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.385
更新日期：1997-09-01 00:00:00
abstract：:Chronic metabolic acidosis (CMA) is a major cause of growth defect, implying disturbances of protein metabolism. Previously, in vivo studies performed in the fasting state showed enhanced whole body protein turnover, whereas in vitro studies showed unchanged muscle protein synthesis. The present study is the first to ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.472
更新日期：1994-12-01 00:00:00
abstract：:Higher diet-dependent nonvolatile acid load is associated with faster chronic kidney disease (CKD) progression, but most studies have used estimated acid load or measured only components of the gold standard, net acid excretion (NAE). Here we measured NAE as the sum of urine ammonium and titratable acidity in 24-hour ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.09.012
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND:Atherosclerotic vascular disease is the leading cause of death in patients with diabetes mellitus and end-stage renal disease. Advanced glycation end products (AGEs) are strongly suggested to be involved in the pathogenesis of atherosclerosis in these patients who also frequently experience infectious compli...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00157.x
更新日期：1998-11-01 00:00:00
abstract：:Many xenobiotics including the pharmacoenhancer cobicistat increase serum creatinine by inhibiting its renal active tubular secretion without affecting the glomerular filtration rate. This study aimed to define the transporters involved in creatinine secretion, applying that knowledge to establish the mechanism for xe...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2014.66
更新日期：2014-08-01 00:00:00
abstract：:The improvement in the anemia in patients with end-stage renal disease (ESRD) on continuous ambulatory peritoneal dialysis (CAPD) suggests that dialyzable substances present in the sera of uremic patients either inhibit erythropoiesis directly or inactivate erythropoietin (EPO). In the present study predialysis sera f...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.88
更新日期：1991-04-01 00:00:00
abstract：:We report the stepwise application of the RIFLE classification in 155,624 admissions in the UK Intensive Care National Audit & Research Centre Case Mix Programme database. The assumptions required to define RIFLE and their relationship with renal replacement therapy (RRT) and ICU mortality were assessed. Previous repo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2015.70
更新日期：2015-08-01 00:00:00
abstract：BACKGROUND:Atherogenic lipoproteins cause injury to the vascular wall in the early phase of atherogenesis. We assessed the effects of native (nLDL) and oxidized (oxLDL) low-density lipoprotein (LDL) and lipoprotein (a) [Lp(a)] on O2- formation and cell death in cultured human umbilical vein endothelial cells (HUVECs) a...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00351.x
更新日期：1999-04-01 00:00:00
abstract：BACKGROUND:Cellular and molecular mechanisms responsible for cisplatin-induced nephrotoxicity to renal tubular epithelial cells are not well understood. Although caspases play a critical role in the execution of the cell death pathway, their specific role in toxic injury to renal tubular epithelial cells has not been e...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00026.x
更新日期：2001-11-01 00:00:00
abstract：:Low-protein diets (LPD) increase insulin-mediated glucose disposal in chronic renal failure (CRF), but the fate of the better utilized glucose and the effect on energy production rate are unknown. Using a two-step (1 and 5 mU x kg(-1) x min(-1)) euglycemic hyperinsulinemic clamp combined with indirect calorimetry, we ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.167
更新日期：1997-04-01 00:00:00
abstract：:Fourteen Center hemodialysis (CHD) patients, 13 continuous ambulatory peritoneal dialysis (CAPD) patients, and 10 normals matched by group for age and sex were compared using a battery of evoked (EP) and event-related (ERP) brain potential measures to determine the effects on central nervous system (CNS) functioning o...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1986.279
更新日期：1986-12-01 00:00:00
abstract：BACKGROUND:Fractalkine is a newly identified T-cell and monocyte/macrophage (Mphi) chemokine with a transmembrane domain and is a cell-surface protein on activated endothelium. It can mediate adhesion of cells expressing the fractalkine receptor CX3CR1. These unique features make fractalkine well suited for leukocyte r...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00604.x
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND:Cux-1 is a murine homeobox gene that is highly expressed in the nephrogenic zone of the developing kidney. Transgenic mice ectopically expressing Cux-1 develop renal hyperplasia associated with down-regulation of the cyclin kinase inhibitor p27. Because the reduction of p27 has been associated with mesangial...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00889.x
更新日期：2003-04-01 00:00:00
abstract：:Hypertriglyceridemia in nephrotic (NS) and Nagase analbuminemic rats (Analb) results from reduced triglyceride clearance. NS and Analb have reduced or absent albumin, reduced plasma oncotic pressure (pi), but Analb lack proteinuria. The heparin releasable lipoprotein lipase (LpL) pool in both models is greatly reduced...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000318
更新日期：2006-08-01 00:00:00
abstract：:Fibroblasts in parenchymal organs potentially contribute extracellular matrix to local fibrogenic processes. This contribution, in some circumstances, may be initiated by cytokines disseminated from inflammatory lesions. Different populations of fibroblasts, however, might respond distinctively to this cytokine bath d...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.3
更新日期：1992-01-01 00:00:00
abstract：:Spleen tyrosine kinase (SYK) is an important component of the intracellular signaling pathway for various immunoreceptors. Inhibition of SYK has shown promise in preclinical models of autoimmune and glomerular disease. However, the description of SYK expression in human renal tissue, which would be desirable ahead of ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2015.29
更新日期：2015-07-01 00:00:00
abstract：:A new study by Xu et al. presents compelling evidence for an important role of the glomerular endothelium in acute kidney injury. They show that lipopolysaccharide reduces the endothelial surface layer, resulting in mild albuminuria, reduced glomerular filtration rate, and fewer endothelial fenestrae. Tumor necrosis f...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2013.317
更新日期：2014-01-01 00:00:00
abstract：:Meprins are zinc-dependent metalloproteinases that are highly expressed in the brush-border membranes of both the kidney and the intestines. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. In this ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002189
更新日期：2007-05-01 00:00:00
abstract：:Recent studies have greatly expanded our knowledge of initial events that lead to epithelial cell polarity. Epithelial polarity is defined, in part, by apical cell-cell tight junctions that separate the plasma membrane into the apical domain and the basolateral domain, as well as the zonula adherens that mediate inter...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5002579
更新日期：2007-12-01 00:00:00
abstract：:Administration of antibody, directed against glomerular basement membrane (GBM) heparan sulfate-proteoglycan, into a presensitized rat results in the induction of membranous nephropathy with subepithelial immune-complex deposits. In this investigation, we examined the mechanisms responsible for the formation of subepi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.166
更新日期：1988-08-01 00:00:00
abstract：:The effects of endogenous and exogenous glucocorticoids on renal active and prokallikrein levels (ng/mg protein) and in vivo kallikrein synthesis rate were studied in the conscious rat. Within two hours after low dose methylprednisolone (MP, 0.0125 to 0.05 mg/100 g body wt), active kallikrein and prokallikrein fell (2...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1990.188
更新日期：1990-08-01 00:00:00
abstract：:Macrophages are inflammatory cells with important roles in the propagation of renal injury. More recently they have also been shown to be important in the resolution of inflammation. Wang et al. show that macrophages can be modulated ex vivo by cytokine stimulation, localize to renal tissue, and slow progression of ex...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/sj.ki.5002332
更新日期：2007-08-01 00:00:00
