Abstract：
:Earlier we demonstrated that meta-iodobenzylguanidine (MIBG), a specific inhibitor of arginine mono-ADP-ribosylation blocks proliferation and differentiation of chick skeletal myogenic cells in culture (Exp. Cell Res., 1992, 201:33-42). Membrane fractions from 4-day, myotube cultures of embryonic chick muscle cells were incubated with 32P-NAD+. Several proteins were labeled, but labeling of two hands of about 53 and 36 kDa appeared to be due to arginyl ADP-ribosylation. Immunoprecipitation with D3 monoclonal antibody to the intermediate filament protein desmin, SDS-PAGE and autoradiography demonstrated that the 53 kDa band contained desmin, and that this desmin is ADP-ribosylated by the endogenous arginine-specific mono(ADP-ribosyl)transferase (Exp. Cell Res., 1996, in press). Desmin is the muscle-specific intermediate filament protein, and it appears to be one of the first muscle-specific proteins expressed during terminal myogenic differentiation. We have examined whether desmin can be ADP-ribosylated in muscle cells by use of polyclonal antibodies for ADP-ribosylated arginyl residues. We have found that soluble desmin is present in 5-6 day myogenic cell cultures and that this desmin contains ADP-ribose, demonstrating that desmin is ADP-ribosylated in skeletal muscle cells. We also found that purified avian desmin contains antigenic material that reacts with these antibodies. In both cases, NaCl had no effect on the reactivity, but NH2OH did, which is consistent with an arginine-ADPR linkage. In summary, these results suggest that ADP-ribosylation is an important regulatory mechanism in differentiating muscle cells, and that the intermediate filament protein desmin is an important substrate for modification in muscle cells.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Graves DJ,Huiatt TW,Zhou H,Huang HY,Sernett SW,Robson RM,McMahon KKdoi
10.1007/978-1-4419-8632-0_40subject
Has Abstractpub_date
1997-01-01 00:00:00pages
305-13eissn
0065-2598issn
2214-8019journal_volume
419pub_type
杂志文章abstract：:Gene therapy delivery using viral vectors has demonstrated efficient transfection but has safety issues. The need for safer yet effective delivery systems has led to the active development of non-viral techniques. In this chapter, we will discuss two evolving techniques - sonoporation with microbubble contrast agents ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4471-6458-6_12
更新日期：2014-01-01 00:00:00
abstract：:Diseases can be caused naturally by biological agents such as bacteria, viruses and toxins (natural risk). However, such biological agents can be intentionally disseminated in the environment by a State (military context) or terrorists to cause diseases in a population or livestock, to destabilize a nation by creating...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-72077-7_9
更新日期：2017-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are multipotent stem cells that reside in various organs. They have the capacity to differentiate into various cell types, including cardiomyocytes, vascular endothelial cells, and vascular smooth muscle cells. Among the various MSCs, bone marrow-derived MSCs (BMMSCs) have been widely use...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-10-4397-0_12
更新日期：2017-01-01 00:00:00
abstract：:MP4CO, developed by Sangart Inc. (San Diego, CA), is a pegylated human hemoglobin-based carbon monoxide (CO) delivery agent and oxygen therapeutic that has shown potential to prevent and reverse red cell sickling. A double blind, comparator controlled, dose-escalation, Phase 1b study was conducted to assess the safety...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,多中心研究,随机对照试验
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abstract：:Substrate/inhibitor specificities of nucleoside analogues with modified sugar moieties toward highly purified deoxycytidine kinase (dCK) and thymidine kinases (TK1 and TK2) from human leukemic spleen have been examined. Substrate activities of cytosine nucleosides vs dCK were as follows: 2'-fluoro-dC > 2'-O-methyl-C >...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-5381-6_120
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abstract：:Embryo implantation requires a reciprocal interaction between the blastocyst and endometrium and is associated with complex regulatory mechanisms. Since their discovery, microRNAs became prominent candidates providing missing links for many biological pathways. In recent years, microRNAs were implicated as one of the ...
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pub_type： 杂志文章
doi：10.1007/978-3-319-22380-3_8
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abstract：:Helicobacter pylori infection of the human stomach is associated with chronic gastritis, peptic ulcer disease or gastric carcinoma, and thus a high burden for the public health systems worldwide. Fortunately, only a small subfraction of up to 15-20% of infected individuals will develop serious complications. Unfortuna...
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pub_type： 杂志文章,评审
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abstract：:Official mortality statistics provide population-based data and serve to improve epidemiological knowledge of rare diseases (RDs), by helping with the description of the natural history of the disease. They are an important complement of registries and estimates of disease burden and costs. At the same time, they heig...
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abstract：:Stroke-prone spontaneously hypertensive rats (SHRSP) are liable to suffer a stroke between 25 and 40 weeks of age. Left ventricular capillarity was studied in 40-week-old SHRSP to clarify the effects of hypertrophic changes in cardiomyocytes on oxygen transport capacity within the tissue. The innermost region of the l...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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abstract：:The role of prolactin and of estradiol and progesterone in the control of the biosynthetic and secretory activity of TIDA neurons has been investigated in the following animal models: young female rats, aged female rats, and young male rats. The indices of TIDA neuronal function employed were a) mass of TH in neurites...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4684-5799-5_1
更新日期：1990-01-01 00:00:00
abstract：:Cognitive-behavioral therapy (CBT) is the main method of psychotherapy generally accepted in the field of substance addiction and non-substance addiction. This chapter mainly introduces the methods and technology of cognitive-behavior therapy of substance addiction, especially in order to prevent relapse. In the cogni...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-10-5562-1_16
更新日期：2017-01-01 00:00:00
abstract：:It is well established that palmitoylation plays a key role in the regulation of immune checkpoints, but the technical challenges in detecting protein palmitoylation have significantly prohibited further researches in this field. Till now, different approaches have been proposed, such as mutagenesis, antibody-based me...
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doi：10.1007/978-981-15-3266-5_17
更新日期：2020-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-0-387-72005-0_14
更新日期：2007-01-01 00:00:00
abstract：:There is very little direct research to conclusively prove the relevance of diet in primary tendinopathies, however it seems prudent to ask whether our current knowledge about the impact of nutrition on collagen metabolism could be useful in assessing, preventing, or treating tendinopathy. The objective of this chapte...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-33943-6_27
更新日期：2016-01-01 00:00:00
abstract：:A new approach to measure muscle oxygen saturation (SmO(2)) using near infrared spectroscopy (NIRS) has been proposed in this paper. This approach exploits the cyclic NIRS signals seen during exercise which are often regarded as "movement artefacts". This new measure, which we term the "cyclic SmO(2)", has the potenti...
journal_title：Advances in experimental medicine and biology
pub_type： 临床试验,杂志文章
doi：10.1007/978-1-4419-1241-1_26
更新日期：2010-01-01 00:00:00
abstract：:This study investigated the relationships between blood pressure, cortical oxygen pressure and hydroxyl radicals in the brain of adult cats during hemorrhagic hypotension and retransfusion. Oxygen pressure in the blood of the cortex was measured optically by the oxygen dependent quenching of phosphorescence and hydrox...
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pub_type： 杂志文章
doi：10.1007/978-1-4615-4863-8_21
更新日期：1998-01-01 00:00:00
abstract：:Two species of T-kininogen which release T-kinin (Ile-Ser-bradykinin) have been purified from plasma of rats treated with Freund's complete adjuvant. The molecular weight was estimated to be 69,000 for either T-kininogen I and II by SDS-polyacrylamide gel electrophoresis. Trypsin released one mole of T-kinin from one ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5143-6_10
更新日期：1986-01-01 00:00:00
abstract：:Biobanking of human biological specimens has evolved from the simple private collection of often poorly annotated residual clinical specimens, to well annotated and organized collections setup by commercial and not-for-profit organizations. The activities of biobanks is now the focus of international and government ag...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2015-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-10-5526-3_2
更新日期：2018-01-01 00:00:00
abstract：:The number of helminths within a host can only increase by the host encountering additional infectious stages, so it is important to consider not only whether a host is infected, but also the severity of its infection. Stochastic models consider explicitly the number of parasites within the host and treat infection, d...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-6064-1_5
更新日期：2010-01-01 00:00:00
abstract：:This review covers recent advances in a variety of dyskinesias. Introduction of new drugs for the treatment of myoclonus and sensory biofeedback therapy for focal dystonia are expanding our concepts of these types of movement disorders. Progress in the treatment of action myoclonus is especially noteworthy and has led...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-2511-6_7
更新日期：1977-01-01 00:00:00
abstract：:Intact and viable parenchymal and non-parenchymal liver cell preparations were isolated by enzyme perfusion techniques from young and old rats. The distribution of the lysosomal enzymes acid phosphatase, beta-galactosidase, cathepsin D, acid DNAse, and arylsulphatase B over parenchymal and non-parenchymal cells was de...
journal_title：Advances in experimental medicine and biology
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abstract：:Store-operated Ca(2+) entry (SOCE) is activated in response to depletion of the ER-Ca(2+) stores by the ER Ca(2+) sensor protein, STIM1 which oligomerizes and moves to ER/PM junctional domains where it interacts with and activates channels involved in SOCE. Two types of channel activities have been described. I(CRAC),...
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pub_type： 杂志文章,评审
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更新日期：2011-01-01 00:00:00
abstract：:The mammalian genome is believed to contain some 30,000 to 40,000 different genes. Of these an estimated 42% have no known function. Genetically engineered mouse models (GEMM) have been a powerful tool available for determining gene function in vivo. In the mammary gland, a variety of genetic engineering approaches ha...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2004-01-01 00:00:00
abstract：:In summary, NO is capable of decreasing mitochondrial respiration in a variety of mammalian tissues. This effect is mediated primarily via binding of NO to the O2 binding site of cytochrome oxidase. This highly sensitive interaction presumably reflects a remnant homology between cytochrome oxidase and bacterial nitrat...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-4717-4_46
更新日期：1999-01-01 00:00:00
abstract：:Schizophrenia is a severe and debilitating psychiatric disorder believed to have neurodevelopmental origins. Several studies have associated energy metabolism dysfunction with the disorder, mostly related to glycolysis alterations. Glucose is the obligatory energy substrate of the brain and glycolysis is the first ste...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
abstract：:In studies designed to determine the respective functional roles of two bulbospinal monoaminergic pathways to sympathetic preganglionic neurons, both L-dopa and precursors of 5-HT depressed transmission through excitatory spinal reflex and bulbospinal sympathetic pathways. Transmission through spinal reflex pathways w...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-2511-6_13
更新日期：1977-01-01 00:00:00
abstract：:The phylum Cnidariais one of the earliest branches in the animal tree of life providing crucial insights into the early evolution of immunity. The diversity in cnidarian life histories and habitats raises several important issues relating to immunity. First, in the absence of specific immune cells, cnidarians must hav...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-8059-5_1
更新日期：2010-01-01 00:00:00
abstract：:The formation of meat mutagens has been studied the last 10 years by carrying out modeling and meat cooking experiments in parallel. During this time, the list of meat mutagens has been growing, and continues to grow. The meat mutagens are usually produced in the crust of animal foods during frying, broiling, and baki...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4899-2626-5_7
更新日期：1991-01-01 00:00:00
abstract：:Transcutaneous PO2 measurements at 37 degrees C can recognize and quantify diabetic microangiopathy if suitable stimulation tests are used. The following parameters are of practical importance: resting PO2, postocclusive PO2, rubefacient-induced PO2 and 45 degrees C heating PO2. Patients with diabetic microangiopathy ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4613-1927-6_15
更新日期：1987-01-01 00:00:00
