当前位置： SCI文献检索 > PHYTOCHEMISTRY期刊下所有文献 > Detection of tomatinase from Fusarium oxysporum f. sp. lycopersici in infected tomato plants.

Detection of tomatinase from Fusarium oxysporum f. sp. lycopersici in infected tomato plants.

Abstract：

:The antifungal glycoalkaloid alpha-tomatine of the tomato plant (Lycopersicon esculentum) is proposed to protect the plant against phytopathogenic fungi. Fusarium oxysporum f. sp. lycopersici, a vascular pathogen of tomato, produces a tomatinase enzyme which hydrolyses the glycoalkaloid into non-fungitoxic compounds. Detoxification of alpha-tomatine may be how this fungus avoids the plant glycoalkaloid barrier. As an initial step to evaluate this possibility we have studied the induction of tomatinase; (i) in fungal cultures containing extracts from leaf, stem or root of tomato plants; and (ii) in stem and root of tomato plants infected with the pathogen at different infection stages. The kinetics of tomatinase induction with leaf extract (0.6% dry weight) was similar to that observed with 20 micrograms ml-1 of alpha-tomatine. In the presence of stem extract, tomatinase activity was less than 50% of that induced with leaf extract, whereas in the presence of root extract tomatinase activity was very low. In the stem of infected tomato plants tomatinase activity was higher at the wilt stage than in previous infections stages and in root, tomatinase activity appeared with the first symptoms and was maintained until wilting. TLC analysis showed that the tomatinase induced in culture medium with plant extracts and in infected tomato plants had the same mode of action as the enzyme induced with pure alpha-tomatine, hydrolysing the glycoalkaloid into its non-fungitoxic forms, tomatidine and beta-lycotetraose. The antisera raised against purified tomatinase recognized in extracts of root and stem of infected tomato plants a protein of 50000 (45000 when proteins were deglycosylated), corresponding to the tomatinase enzyme. Therefore, it is concluded that F. oxysporum f. sp. lycopersici express tomatinase in vivo as a result of the infection of tomato plant.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Lairini K,Ruiz-Rubio M

doi

10.1016/s0031-9422(97)00170-2

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

1371-6

issue

7

eissn

0031-9422

issn

1873-3700

pii

S0031-9422(97)00170-2

journal_volume

45

pub_type

杂志文章

相关文献

PHYTOCHEMISTRY文献大全
  • Bioactive terpenoids from sunflower leaves cv. Peredovick.

    abstract：:The CH(2)Cl(2) extract of dried leaves of Helianthus annuus L. cv. Peredovick(R) has yielded, in addition to the known sesquiterpene lactones annuolide E and leptocarpin, and the sesquiterpenes heliannuols A, C, D, F, G, H, I, the new bisnorsesquiterpene, annuionone E, and the new sesquiterpenes heliannuol L, helibisa...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(02)00370-9

    authors： Macías FA,Torres A,Galindo JL,Varela RM,Alvarez JA,Molinillo JM

    更新日期：2002-11-01 00:00:00

  • Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates.

    abstract：:The role and fate of Jasmonoyl-Phenylalanine (JA-Phe), an understudied conjugate in the jasmonate pathway remain to be unraveled. We addressed here the possibility of JA-Phe oxidative turnover by cytochrome P450s of the CYP94 family. Leaf wounding or fungal infection in Arabidopsis resulted in accumulation of JA-Phe, ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2015.06.027

    authors： Widemann E,Grausem B,Renault H,Pineau E,Heinrich C,Lugan R,Ullmann P,Miesch L,Aubert Y,Miesch M,Heitz T,Pinot F

    更新日期：2015-09-01 00:00:00

  • Evolutionary changes in defensive specialized metabolism in the genus Hordeum.

    abstract：:Plants have developed defensive specialized metabolites over the course of evolution. In the genus Hordeum, which includes the important cereal crop barley, specialized metabolites such as hordatines, benzoxazinones, and gramine have been identified. Hordeum species are classified into four clades, H, Xu, Xa, and I. T...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2017.05.004

    authors： Ube N,Nishizaka M,Ichiyanagi T,Ueno K,Taketa S,Ishihara A

    更新日期：2017-09-01 00:00:00

  • Covalent binding of chloroacetamide herbicides to the active site cysteine of plant type III polyketide synthases.

    abstract：:Chloroacetamide herbicides inhibit very-long-chain fatty acid elongase, and it has been suggested that covalent binding to the active site cysteine of the condensing enzyme is responsible [Pest Manage Sci 56 (2000), 497], but direct evidence was not available. The proposal implied that other condensing enzymes might a...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(03)00516-8

    authors： Eckermann C,Matthes B,Nimtz M,Reiser V,Lederer B,Böger P,Schröder J

    更新日期：2003-11-01 00:00:00

  • Purification and spectroscopic studies on catechol oxidase from lemon balm (Melissa officinalis).

    abstract：:A catechol oxidase from lemon balm (Melissa officinalis) moCO which only catalyzes the oxidation of catechols to quinones without hydroxylating tyrosine was purified. The molecular mass of the M. officinalis enzyme of 39,370 Da was obtained by MALDI mass spectrometry and the isoelectric point was determined to be 3.4....

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2012.05.022

    authors： Rompel A,Büldt-Karentzopoulos K,Molitor C,Krebs B

    更新日期：2012-09-01 00:00:00

  • The C-terminal zinc finger domain of Arabidopsis cold shock domain proteins is important for RNA chaperone activity during cold adaptation.

    abstract：:Among the four cold shock domain proteins (CSDPs) identified in Arabidopsis thaliana, it has recently been shown that CSDP1 harboring seven CCHC-type zinc fingers, but not CSDP2 harboring two CCHC-type zinc fingers, function as a RNA chaperone during cold adaptation. However, the structural features relevant to this d...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2009.12.006

    authors： Park SJ,Kwak KJ,Jung HJ,Lee HJ,Kang H

    更新日期：2010-04-01 00:00:00

  • Purification and characterization of a protein phosphatase from winged bean.

    abstract：:A protein phosphatase (WbPP) has been purified from the soluble fraction of the winged bean (Psophocarpus tetragonolobus) shoot extract. The preparation is essentially homogenous as shown by the constant specific activity of the enzyme across the peak fractions, eluted from the thiophosphorylated histone-Sepharose aff...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(98)00473-7

    authors： Ganguly S,Singh M

    更新日期：1999-09-01 00:00:00

  • Pongamone A-E, five flavonoids from the stems of a mangrove plant, Pongamia pinnata.

    abstract：:Chemical investigation of stems of the mangrove plant, Pongamia pinnata, resulted in isolation and characterization of five structurally unusual flavonoids pongamones A-E, along with 16 known flavonoid metabolites. Their structures were determined on the basis of spectroscopic analyses and by comparison of their spect...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2006.05.016

    authors： Li L,Li X,Shi C,Deng Z,Fu H,Proksch P,Lin W

    更新日期：2006-07-01 00:00:00

  • Strigol: biogenesis and physiological activity.

    abstract：:The role played by molecules of the strigolactone family in stimulating the germination of seeds of parasitic weeds of the genera Striga, Orobanche and Alectra has never been clearly elucidated. The biogenesis of these unusual terpenoid lactones, originally identified in minute quantities in the root exudates of a sma...

    journal_title：Phytochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.phytochem.2005.12.026

    authors： Humphrey AJ,Beale MH

    更新日期：2006-04-01 00:00:00

  • NMR characterization of hydration and thermal stress in tomato fruit cuticles.

    abstract：:In its natural environment, the plant cuticle, which is composed of the biopolymer cutin and a mixture of surface and embedded cuticular waxes, experiences a wide variety of temperatures and hydration states. Consequently, a complete understanding of cuticular function requires study of its thermal and mechanical prop...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2008.08.016

    authors： Stark RE,Yan B,Stanley-Fernandez SM,Chen ZJ,Garbow JR

    更新日期：2008-11-01 00:00:00

  • The structures of antioxidant and cytotoxic agents from natural source: anthraquinones and tannins from roots of Rumex patientia.

    abstract：:A new anthraquinone glycoside, emodin-6-O-beta-D-glucopyranoside, and a new simple halogenated flavan-3-ol, 6-chlorocatechin, have been isolated from the roots of Rumex patientia L. together with seven known phenolic compounds. Their structures were elucidated on the basis of spectroscopic methods. Cytotoxic effects a...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(01)00337-5

    authors： Demirezer LO,Kuruüzüm-Uz A,Bergere I,Schiewe HJ,Zeeck A

    更新日期：2001-12-01 00:00:00

  • Chemical and genetic diversity of Ligularia vellerea in Yunnan, China.

    abstract：:Intra-specific diversity in Liularia vellerea growing in the northwestern to central Yunnan province of China was studied by chemical and genetic approaches. Samples collected in the Jianchuan, Lijiang, and Zhongdian areas contained 6,15-dioxygenated furanoeremophilanes as their major components (type A); whereas samp...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2007.12.001

    authors： Tori M,Nakamizo H,Mihara K,Sato M,Okamoto Y,Nakashima K,Tanaka M,Saito Y,Sono M,Gong X,Shen Y,Hanai R,Kuroda C

    更新日期：2008-03-01 00:00:00

  • Kudzu root: an ancient Chinese source of modern antidipsotropic agents.

    abstract：:Kudzu (Pueraria lobata) is one of the earliest medicinal plants used in traditional Chinese medicine. It has many profound pharmacological actions including antidipsotropic (antialcohol abuse) activity. Although both the roots and flowers of kudzu, Radix and Flos puerariae, respectively, have been used to treat alcoho...

    journal_title：Phytochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/s0031-9422(97)00723-1

    authors： Keung WM,Vallee BL

    更新日期：1998-02-01 00:00:00

  • Triterpene saponins from Eryngium kotschyi.

    abstract：:Four new oleanane-type saponins 3-O-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucuronopyranosyl-22-O-β,β-dimethylacryloylA1-barrigenol (1), 3-O-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucuronopyranosyl-22-O-angeloylA1-barrigenol (2), 3-O-β-D-glucopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranosyl-21,22,28-O-triacetyl-(...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2014.11.017

    authors： Aslan Erdem S,Mitaine-Offer AC,Miyamoto T,Kartal M,Lacaille-Dubois MA

    更新日期：2015-02-01 00:00:00

  • COX-2 inhibitory activity of homoisoflavanones and xanthones from the bulbs of the Southern African Ledebouria socialis and Ledebouria ovatifolia (Hyacinthaceae: Hyacinthoideae).

    abstract：:The bulbs of Ledebouria socialis (Hyacinthaceae) yielded the benzocyclobutene homoisoflavonoid, (R)-2',5-dihydroxy-3',4',7-trimethoxyspiro{2H-1-benzopyran-3-(4H)-9-bicyclo[4.2.0]octa[1,3,5]triene}-4-one, socialinone (1). Ledebouria ovatifolia yielded (2ε,3R)-2,5-dihydroxy-7-methoxyspiro[2H-1-benzopyran-3(4H), 5'(6'H)-...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2013.06.024

    authors： Waller CP,Thumser AE,Langat MK,Crouch NR,Mulholland DA

    更新日期：2013-11-01 00:00:00

  • Flavonoid gene expression and UV photoprotection in transgenic and mutant Petunia leaves.

    abstract：:The effects of UVB radiation on plant growth rate, gene expression and flavonoid content in wild-type, and in transgenic and mutant F3'H deficient Petunia lines have been studied for the first time. In wild-type Petunia, UVB induced an increase in total levels of flavonols and this was due to an up-regulation of sever...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(01)00404-6

    authors： Ryan KG,Swinny EE,Markham KR,Winefield C

    更新日期：2002-01-01 00:00:00

  • Metabolic differentiations and classification of Verbascum species by NMR-based metabolomics.

    abstract：:The genus Verbascum L. (mulleins) comprises of about 360 species of flowering plants in the Scrophulariaceae family. Mulleins have been used in the traditional folk medicine for centuries, for treatment of a wide range of human ailments, inter alia bronchitis, tuberculosis, asthma, and different inflammations. Despite...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2011.07.005

    authors： Georgiev MI,Ali K,Alipieva K,Verpoorte R,Choi YH

    更新日期：2011-11-01 00:00:00

  • Three types of sesquiterpenes from rhizomes of Atractylodes lancea.

    abstract：:Eight sesquiterpenes, including four guaiane-types containing an interesting epoxy unit (1-4), a rare tricyclic carbon skeleton-type (5) and three eudesmane-types (6-8), along with five known compounds, were isolated from rhizomes of Atractylodes lancea. The structures and relative configurations of 1-8 were determine...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2008.04.008

    authors： Wang HX,Liu CM,Liu Q,Gao K

    更新日期：2008-07-01 00:00:00

  • 21beta-Hydroxy-oleanane-type triterpenes from Hippocratea excelsa.

    abstract：:Stem bark of Hippocratea excelsa afforded six pentacyclic triterpenes, five oleanane and one ursane types. They were identified as 11beta,21beta-dihydroxy-olean-12-ene-3-one (2), 3alpha,11alpha,21beta-trihydroxy-olean-12-ene (3), 3alpha,21beta-dihydroxy-11alpha-methoxy-olean-12-ene (4), 3alpha,21beta-dihydroxy-olean-9...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2007.10.016

    authors： Cáceres-Castillo D,Mena-Rejón GJ,Cedillo-Rivera R,Quijano L

    更新日期：2008-02-01 00:00:00

  • A role for a Hevea latex lectin-like protein in mediating rubber particle aggregation and latex coagulation.

    abstract：:An in vitro aggregation of washed lutoid membrane and rubber particles, respectively, prepared from the bottom (lutoid) fraction and rubber layer of centrifuged fresh latex, leading to the formation of rubber coagulum necessary for a latex coagulation was demonstrated. A Triton X-100 extract of washed lutoid membrane ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2007.08.019

    authors： Wititsuwannakul R,Pasitkul P,Kanokwiroon K,Wititsuwannakul D

    更新日期：2008-01-01 00:00:00

  • Dual metabolomics: a novel approach to understanding plant-pathogen interactions.

    abstract：:One of the most well-characterised plant pathogenic interactions involves Arabidopsis thaliana and the bacteria Pseudomonas syringae pathovar tomato (Pst). The standard Pst inoculation procedure involves infiltration of large populations of bacteria into plant leaves which means that metabolite changes cannot be readi...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2010.01.006

    authors： Allwood JW,Clarke A,Goodacre R,Mur LA

    更新日期：2010-04-01 00:00:00

  • Antimicrobial metabolites produced by an intertidal Acremonium furcatum.

    abstract：:In a screening for antimicrobial metabolites, amides of D-allo- and L-isoleucine derivatives were isolated from the culture of a marine strain of Acremonium furcatum. Structural elucidation of these compounds was performed by analysis of spectroscopic data and confirmed by synthesis. All of the compounds, natural and ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2006.07.028

    authors： Gallardo GL,Butler M,Gallo ML,Rodríguez MA,Eberlin MN,Cabrera GM

    更新日期：2006-11-01 00:00:00

  • The microbiological transformation of 7alpha-hydroxy-ent-kaur-16-ene derivatives by Gibberella fujikuroi.

    abstract：:The biotransformation of 7alpha-hydroxy-ent-kaur-16-ene (epi-candol A) by the fungus Gibberella fujikuroi gave 7alpha,16alpha,17-trihydroxy-ent-kaur-16-ene and a seco-ring B derivative, fujenoic acid, whilst the incubation of candicandiol (7alpha,18-dihydroxy-ent-kaur-16-ene) and canditriol (7alpha,15alpha,18-trihydro...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2007.03.024

    authors： Fraga BM,Bressa C,González P,Guillermo R,Hernández MG,Suárez S

    更新日期：2007-06-01 00:00:00

  • Coumaronochromones and flavanones from Euchresta formosana roots.

    abstract：:Four coumaronochromones, formosanatins A-D (-4), and a flavanone, euchrenone a(16), along with four known compounds, euchretins E and G, euchrestaflavanone A, and daidzein, were isolated from the roots of Euchresta formosana. The structures of - were established by spectroscopic analyses. ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(02)00017-1

    authors： Lo WL,Chang FR,Hsieh TJ,Wu YC

    更新日期：2002-08-01 00:00:00

  • A proteomic approach to studying plant response to crenate broomrape (Orobanche crenata) in pea (Pisum sativum).

    abstract：:Crenate broomrape (Orobanche crenata) is a parasitic plant that threatens legume production in Mediterranean areas. Pea (Pisum sativum) is severely affected, and only moderate levels of genetic resistance have so far been identified. In the present work we selected the most resistant accession available (Ps 624) and c...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2004.03.029

    authors： Angeles Castillejo M,Amiour N,Dumas-Gaudot E,Rubiales D,Jorrín JV

    更新日期：2004-06-01 00:00:00

  • Camptothecin, over four decades of surprising findings.

    abstract：:Camptothecin (CPT) is a modified monoterpene indole alkaloid produced by Camptotheca acuminata (Nyssaceae), Nothapodytes foetida, Pyrenacantha klaineana, Merrilliodendron megacarpum (Icacinaceae), Ophiorrhiza pumila (Rubiaceae), Ervatamia heyneana (Apocynaceae) and Mostuea brunonis (Gelsemiaceae), species belonging to...

    journal_title：Phytochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.phytochem.2004.09.001

    authors： Lorence A,Nessler CL

    更新日期：2004-10-01 00:00:00

  • Characterisation of triterpenes and new phenolic lipids in Cameroonian propolis.

    abstract：:Chemical investigation of a sample of propolis originating from North-Western Cameroon led to the isolation of thirteen alk(en)ylphenols (1-13) (inseparable mixture) along with α-amyrin (14), β-amyrin (15), lupeol (16), cycloartenol (17), mangiferonic acid (18), ambonic acid (19), mangiferolic acid (20), ambolic acid ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2014.07.016

    authors： Kardar MN,Zhang T,Coxon GD,Watson DG,Fearnley J,Seidel V

    更新日期：2014-10-01 00:00:00

  • Triterpene saponins from Scabiosa rotata.

    abstract：:Four new allose-containing triterpenoid saponosides, scabriosides A, B, C and D were isolated from the roots of Scabiosa rotata. Their structures were established as 3-O-beta-D-xylopyranosyl-28-O-[beta-D-allopyranosyl (1-->6)-beta-D-glucopyranosyl]-pomolic acid, 3-O-[alpha-L-rhamnopyranosyl (1-->2)-beta-D-xylopyranosy...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(97)00982-5

    authors： Baykal T,Panayir T,Tasdemir D,Sticher O,Calis I

    更新日期：1998-07-01 00:00:00

  • Profile and quantification of glucosinolates in Pentadiplandra brazzeana Baillon.

    abstract：:Glucosinolates (GLs) present in root, seed, and leaf extracts of Pentadiplandra brazzeana Baillon were characterized and quantified according to the ISO 9167-1 method based on the HPLC analysis of desulfo-GLs. The analyses were complemented by GC-MS analyses of the isothiocyanates (ITCs) generated from GL degradation ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2011.09.006

    authors： De Nicola GR,Nyegue M,Montaut S,Iori R,Menut C,Tatibouët A,Rollin P,Ndoyé C,Zollo PH

    更新日期：2012-01-01 00:00:00

  • Polyoxygenated cyclohexene derivatives from Ellipeiopsis cherrevensis.

    abstract：:Aerial parts of Ellipeiopsis cherrevensis contained the polyoxygenated cyclohexenes zeylenol, ferrudiol and three analogs, ellipeiopsols A, B and C. The C-1 stereochemistry of ferrudiol has been revised. ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(01)00465-4

    authors： Kijjoa A,Bessa J,Pinto MM,Anatachoke C,Silva AM,Eaton G,Herz W

    更新日期：2002-03-01 00:00:00