Influence of postischemic administration of oxyradical antagonists on ischemic injury to rabbit skeletal muscle.

Abstract:

:The aim of this study was to determine whether the administration of free radical antagonists, immediately before and during the early minutes of reperfusion, improves muscle survival 24 hr after a period of ischemia. Rabbit rectus femoris muscles were isolated, made ischemic for 3 1/2 hr and treated with either desferrioxamine (DFX), an Fe3+ chelator, superoxide dismutase and catalase (SOD & CAT), which quench superoxide and hydrogen peroxide, or allopurinol, an inhibitor of xanthine oxidase (XO). After 24 hr reperfusion, muscle viability (+/-s.e.m.), measured by the nitro blue tetrazolium (NBT) vital staining technique, was 41.6 +/- 11.3% for saline-treated ischemic controls, 30.6 +/- 7.6% for DFX-treated, 46.7 +/- 10.3% for SOD & CAT-treated, and 43.3 +/- 9.5% for allopurinol-treated muscles. None of the treated groups differed significantly from the ischemic control group. Tissue myeloperoxidase, ATP and reduced glutathione levels, and plasma lactate dehydrogenase (LDH) and aspartate transaminase (AST) levels were increased by ischemia and reperfusion in all groups, but the changes did not differ between the treatment groups. Levels of XO in the rabbit muscle were determined and found to be very low in both normal and postischemic muscle. As XO is the target enzyme of allopurinol, its absence provides a basis for the lack of effect of this agent. However, it is not clear why DFX and SOD & CAT had no protective effect.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Hickey MJ,Knight KR,Lepore DA,Hurley JV,Morrison WA

doi

10.1002/(SICI)1098-2752(1996)17:9<517::AID-MICR7>3

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

517-23

issue

9

eissn

0738-1085

issn

1098-2752

pii

10.1002/(SICI)1098-2752(1996)17:9<517::AID-MICR7>3

journal_volume

17

pub_type

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