Allopeptide-pulsed dendritic cells and composite tissue allograft survival.

Abstract:

:Composite tissue allografts (CTAs) contain their own reservoir of vascularized bone marrow, offering novel aspects for the induction of donor-specific tolerance. Additionally, the manipulation of recipient dendritic cells, pulsed with donor allopeptide, has been shown to engender solid organ allograft survival. To exploit these modalities, we have developed a protocol utilizing injection of recipient bone marrow-derived dendritic cells (BMDCs) pulsed with a donor-derived peptide for use in CTA transplantation. Six days prior to orthotopic hind-limb transplantation, Lewis rats received IV injection of donor allopeptide-pulsed, recipient BMDCs, in conjunction with a single dose of anti-lymphocyte serum. Control groups displayed signs of allograft rejection within 5 days postoperatively. Animals within the primary experimental cohort demonstrated prolongation of graft survival to an average of 8 days, and exhibited low numbers of donor T cells. The use of BMDCs in conjunction with transient immunosuppression has potential therapeutic application for induction of donor-antigen-specific tolerance to hind limb allografts.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Nguyen VT,Taieb A,Sacks JM,Unadkat JV,Clavijo JA,Kim H,Feili-Hariri M,Lee WP

doi

10.1002/micr.20314

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

105-11

issue

2

eissn

0738-1085

issn

1098-2752

journal_volume

27

pub_type

杂志文章
  • Dexter: a device for the assessment of microsurgical instrumentation and instruction of trainees.

    abstract::We present an easily constructed devise, which allows the testing and comparison of microsurgical apparatus, providing a numerical result for statistical analysis. It also enables the trainee microsurgeon to practise basic microsurgical skills in a safe environment. Construction and uses are discussed. ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2752(1998)18:7<430::aid-micr8>3

    authors: Southern SJ,Ramakrishnan V

    更新日期:1998-01-01 00:00:00

  • The periosteal-cutaneous chimeric medial femoral condyle free flap for subtotal ear reconstruction: A case report.

    abstract::When costal graft is contraindicated or refused by the patient, autologous total/subtotal auricular reconstruction represent a real challenge as limited surgical options has been described. Aim of present report is to offer a novel possible autologous reconstruction of the ear frame using a chimeric free medial femora...

    journal_title:Microsurgery

    pub_type:

    doi:10.1002/micr.30657

    authors: di Summa PG,Sapino G,Zaugg P,Raffoul W,Guillier D

    更新日期:2020-10-01 00:00:00

  • Use of the venous flap for salvage of difficult ring avulsion injuries.

    abstract::Ring avulsion can lead to soft tissue compromise resulting in eventual digit failure or restriction of motion. The authors present use of the venous flow through flap for simultaneous soft tissue and digital vessel reconstruction in severe ring avulsion injury. A retrospective review of ring avulsion injuries treated ...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20527

    authors: Brooks D,Buntic RF,Taylor C

    更新日期:2008-01-01 00:00:00

  • The free fascial forearm flap.

    abstract::The unpleasant appearance of the donor site after harvesting a forearm flap limits its use in many centers. In this paper, the author records his experience with a modification of the standard fasciocutaneous forearm flap. Such modification involves the utilization of the fascial component of the flap sparing the skin...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920100302

    authors: Ismail TI

    更新日期:1989-01-01 00:00:00

  • Secondary ischemic tolerance improved by administration of L-NAME in rat flaps.

    abstract::Nitric oxide (NO) under basal conditions is an important regulator of vascular tone. Under ischemic conditions, however, NO can combine with superoxide anion to produce the damaging hydroxyl free radical. The current project observes the effect of inhibiting NO production (L-Nitro-amino-methyl-arginine, L-NAME) on fla...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2752(1996)17:8<425::AID-MICR1>3

    authors: Knox LK,Angel MF,Gamper T,Amiss LR,Morgan RF

    更新日期:1996-01-01 00:00:00

  • Predictors of plate extrusion in oromandibular free flap reconstruction.

    abstract:OBJECTIVES:Plate extrusions after free tissue transfer for mandibular reconstruction can be problematic and generally require revision surgery. Our objective was to assess the predictors of plate extrusion and compare outcomes between fibular free flaps (FFF), lateral border scapular flaps (LBSF), and scapular tip free...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30349

    authors: Prasad J,Sahovaler A,Theurer J,Yeh DH,Fung K,MacNeil SD,Yoo J,Nichols AC

    更新日期:2018-09-01 00:00:00

  • Microsurgery in microgravity is possible.

    abstract::The aim of this experiment was to evaluate the feasibility of a microsurgical procedure in the context of microgravity during parabolic flights. The surgical procedure included the sectioning and repair of a rat sciatic nerve and tail artery with 10/0 monofilament sutures. Both procedures were successful. To date, tel...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20089

    authors: Pinsolle V,Martin D,de Coninck L,Techoueyres P,Vaïda P

    更新日期:2005-01-01 00:00:00

  • Effect of oblique nerve grafting on peripheral nerve regeneration in rats.

    abstract::Current methods of peripheral nerve repair are to rejoin cut nerve stumps directly or to bridge large gaps with autologous nerve grafts. In both cases the surface of nerve stump endings is typically cut perpendicularly to the long axis of the nerve. The outcome of such operations, however, is still not satisfactory. I...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20290

    authors: Kotulska K,Marcol W,Larysz-Brysz M,Tendera Z,Malinowska-Kołodziej I,Slusarczyk W,Jedrzejowska-Szypułka H,Lewin-Kowalik J

    更新日期:2006-01-01 00:00:00

  • A new technique for microvascular sleeve anastomosis.

    abstract::This study describes our experience using an improved technique for microsurgical sleeve anastomosis of arteries. Modifications of previously described techniques include utilization of a sidecut and placement of the two stitches within separate vertical planes. In 40 arteries, we attained a patency rate of 97.5%. His...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920120502

    authors: Zhang L,Tuchler RE,Shaw WW,Siebert JW

    更新日期:1991-01-01 00:00:00

  • The place of the iliac crest in vascularized oromandibular reconstruction.

    abstract::The iliac crest is reevaluated with respect to the rapid evolution of vascularized mandibular reconstruction. With more flaps to choose from, indications for the crest have become more specific-playing to the strengths of this particular transfer. Ideally, defects should exclude soft tissue and occupy lateral segments...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920150406

    authors: Boyd JB

    更新日期:1994-01-01 00:00:00

  • Natural history of digital replantation: a 12-year prospective study.

    abstract:UNLABELLED:The purpose of the study was to evaluate the fate of the digital arterial anastomoses, resting total digit blood flow, and cold-induced discomfort following digital replantation with two repaired arteries. The evaluation was done with a Doppler ultrasound of the anastomoses, temperature recording from the fi...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920160304

    authors: Povlsen B,Nylander G,Nylander E

    更新日期:1995-01-01 00:00:00

  • Improved techniques for kidney transplantation in mice.

    abstract::Improved microsurgical techniques for kidney transplantation in the mouse are described. Left renal transplantation is performed with end-to-side anastomoses of the donor renal vein to the inferior vena cava and the donor aortic cuff to the aorta. Urinary tract reconstruction is accomplished by suturing the donor uret...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920160212

    authors: Zhang Z,Schlachta C,Duff J,Stiller C,Grant D,Zhong R

    更新日期:1995-01-01 00:00:00

  • On sampling and sampling errors in histomorphometry of peripheral nerve fibers.

    abstract::Histomorphometrical assessment of regenerated peripheral nerves is a very common goal of many studies in experimental microsurgery. In this paper, the main critical issues in nerve fiber sampling for quantitative morphological assessment are addressed. The equal opportunity rule, i.e., the basic paradigm of random sam...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.10199

    authors: Geuna S,Gigo-Benato D,Rodrigues Ade C

    更新日期:2004-01-01 00:00:00

  • Free latissimus dorsi flap with long venous grafts for closure of a soft tissue defect of the spine in a patient with Noonan's syndrome: a case report.

    abstract::A 23-year-old male patient was operated for a soft tissue defect of the spine using a free musculocutaneous latissimus dorsi flap with two 21-cm long venous grafts connecting the flap to its vascular supply at the thoracodorsal vessels. This young patient suffered from Noonan's Syndrome that is a complex hereditary ma...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20625

    authors: Sinis N,Lanaras TI,Kraus A,Werdin F,Schaller HE,Peek A

    更新日期:2009-01-01 00:00:00

  • Pedicled chimeric sensitive fasciocutaneous anterolateral thigh (ALT) and vastus lateralis muscle (VLM) flap for groin defect reconstruction: A case report.

    abstract::The anterolateral thigh (ALT) flap is one of the most commonly used flap worldwide, as both free flap and pedicled local flap. Here, we report the use of a pedicled chimeric sensitive ALT and vastus lateralis muscle (VLM) flap in a patient with a 12 cm × 8 cm contaminated soft tissue defect of the right inguinal regio...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30157

    authors: Scaglioni MF,Franchi A,Giovanoli P

    更新日期:2018-05-01 00:00:00

  • Small bowel transplantation.

    abstract::Small intestinal transplantation has had several clinical attempts based on extensive experimental work during the past two decades. Several models, such as auxiliary (heterotopic) or orthotopic models, have been developed in regard to problems with graft immunology and function. The question of mesenteric-portal or m...

    journal_title:Microsurgery

    pub_type: 杂志文章,评审

    doi:10.1002/micr.1920110411

    authors: Schroeder P,Gundlach M,Schweizer E,Deltz E

    更新日期:1990-01-01 00:00:00

  • Reconstruction of diabetic lower leg and foot soft tissue defects using thoracodorsal artery perforator chimeric flaps.

    abstract:BACKGROUND:Reconstruction of complicated diabetic lower leg and foot defects involving multiple tissue components remains a challenge. The purpose of this report is to introduce thoracodorsal artery perforator (TDAP) chimeric flaps for reconstructing diabetic lower leg and foot soft tissue defects. PATIENTS AND METHOD...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30314

    authors: Sung IH,Jang DW,Kim SW,Kim YH,Kim SW

    更新日期:2018-09-01 00:00:00

  • Free SCIA/SIEA skin flap: a dual blood supply approach to groin region.

    abstract::Free groin flap donor skin is unequaled in generous skin supply and inconspicuousness. We reliably utilize this region by taking advantage of the dual blood supply from superficial circumlex iliac (SCIA) and superficial inferior epigastric (SIEA) arteries and name the flap "Free SCIA/SIEA skin flap." The arterial pedi...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20416

    authors: Aydin MA,Nasir S

    更新日期:2007-01-01 00:00:00

  • Regeneration across cold preserved peripheral nerve allografts.

    abstract::The feasibility of peripheral nerve allograft pretreatment utilizing cold storage (5 degrees C in the University of Wisconsin Cold Storage Solution) or freeze-thawing to prevent rejection was investigated. Regeneration across cold-stored (3 or 5 weeks) or freeze-thawed (FT), 3.0-cm sciatic nerve allografts were compar...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2752(1999)19:3<115::aid-micr1>3

    authors: Evans PJ,MacKinnon SE,Midha R,Wade JA,Hunter DA,Nakao Y,Hare GM

    更新日期:1999-01-01 00:00:00

  • Head and neck reconstruction using cephalic vein transposition in the vessel-depleted neck.

    abstract::In microvascular reconstructive surgery the patency of the recipient vessels is the key to successful outcome. In head and neck surgery there is often a lack of adequate recipient vessels as a result of chemoradiation therapy and ablative surgery. To overcome this it is crucial to identify vessels of adequate length a...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20680

    authors: Vasilakis V,Patel HD,Chen HC

    更新日期:2009-01-01 00:00:00

  • Reconstruction of midfoot bone and soft tissue loss with chimeric partial scapula and latissimus dorsi muscle flap and short perforator-based skin flap following gunshot injuries: Report of two cases.

    abstract:INTRODUCTION:In this report we present two cases of gunshot injury related midfoot defects, reconstructed with a chimeric partial scapula and latissimus dorsi muscle flap and short perforator-based skin flap. The first case, a 14 years old male, had 10 × 8 cm medial plantar and 6 × 4 cm dorsal foot defects and the seco...

    journal_title:Microsurgery

    pub_type:

    doi:10.1002/micr.30099

    authors: Ozcan Akcal A,Ünal K,Gorgulu T,Akif Akcal M,Bigat Z

    更新日期:2016-10-01 00:00:00

  • Improved sensory recovery with a novel dual neurorrhaphy technique for breast reconstruction with free muscle sparing TRAM flap technique.

    abstract:BACKGROUND:We describe a new dual neurorrhaphy method for a free abdominal-based flap and compare sensory recovery with this novel technique to that with conventional neurorrhaphy technique for breast reconstruction. METHODS:70 breast cancer patients underwent muscle sparing innervated transversal rectus abdominis myo...

    journal_title:Microsurgery

    pub_type: 临床试验,杂志文章

    doi:10.1002/micr.30064

    authors: Puonti HK,Jääskeläinen SK,Hallikainen HK,Partanen TA

    更新日期:2017-01-01 00:00:00

  • Vascularized fibular grafting for treatment of aseptic necrosis of the femoral head--preliminary results in four cases.

    abstract::Vascularized grafting with segments of fibula was performed in four men with aseptic necrosis of the femoral head. The patients were restricted to relatively young individuals in whom the necrosis seemed to be confined to two-thirds or less of the femoral head and in whom the outer shell of the femoral head appeared r...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920040108

    authors: Fujimaki A,Yamauchi Y

    更新日期:1983-01-01 00:00:00

  • A novel salvage approach for pedicle axial flap--free-ization of pedicle internal mammary artery perforator flap.

    abstract::A pedicle flap with distal segment compromise is classically managed by allowing tissue demarcation, debridement of non-viable tissue, and local tissue manipulation to achieve wound closure. When aggressive debridement leaves insufficient tissue for defect coverage, the original flap is often discarded. We present a c...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.21962

    authors: Chen WF,Tsao CK,Cheng MH

    更新日期:2012-05-01 00:00:00

  • Effect of vascularized periosteum on revitalization of massive bone isografts: An experimental study in a rabbit model.

    abstract:INTRODUCTION:In the last years, limb salvage has become the gold standard treatment over amputation. Today, 90% of extremity osteogenic sarcomas can be treated with limb salvage surgery. However, these reconstructions are not exempt from complications. Massive allografts have been associated to high risk of nonunion (1...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.30647

    authors: Barastegui D,Gallardo-Calero I,Rodriguez-Carunchio L,Barrera-Ochoa S,Knorr J,Rivas-Nicolls D,Soldado F

    更新日期:2020-09-19 00:00:00

  • Double free-flap reconstruction of massive defects involving the lip, chin, and mandible.

    abstract::Two patients with massive, composite defects of the total lower lip, chin, and anterior mandible underwent double free-flap reconstruction. A fibular osteoseptocutaneous flap was used to reconstruct the mandible and floor of the mouth and a radial forearm fasciocutaneous composite flap, including the palmaris longus t...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2752(1998)18:6<372::aid-micr6>3

    authors: Kuzon WM Jr,Jejurikar S,Wilkins EG,Swartz WM

    更新日期:1998-01-01 00:00:00

  • Free vascularized fibular graft for treatment of pathological femoral neck fracture and osteonecrosis of the femoral head: a case report with a long-term follow-up.

    abstract::We present a case of a 34-year-old white female patient who, 13 years ago, sustained a pathological intracapsular femoral neck fracture on a pre-existing aneurysmal bone cyst. Three months later radiographic and magnetic resonance imaging evaluation revealed both femoral neck fracture and stage IV osteonecrosis of the...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20598

    authors: Beris AE,Lykissas MG,Payatakes A,Kontogeorgakos VA,Mavrodontidis A,Korompilias AV

    更新日期:2009-01-01 00:00:00

  • Heart and spleen "twin grafts" in rats: IV. Influence of donor spleen cell mass on allograft survival.

    abstract::Heart and spleen twin grafts from LEW to ACI rats may survive permanently, whereas those grafted from ACI to LEW rats do not. This strain difference in graft acceptance was analyzed quantitatively by transplanting one to three ACI spleens into LEW rats in order to compensate for the relatively small size of ACI spleen...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.1920040407

    authors: Blanchard JM,Tauber JW,Lazda VA,Mozes MF

    更新日期:1983-01-01 00:00:00

  • Recovery of touch after median nerve lesion and subsequent repair.

    abstract::Many techniques have been developed for the evaluation of peripheral nerve function. Consequently, physicians use different techniques in the clinic. This study describes the evaluation of touch after median nerve lesions in the forearm and repair. In order to evaluate touch, 25 patients, aged 11-51 years (mean, 29 ye...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.10087

    authors: Meek MF,Coert JH,Wong KH

    更新日期:2003-01-01 00:00:00

  • Neuropathological changes in vibration injury: an experimental study.

    abstract::Vibration syndrome, a clinical condition arising from chronic use of vibrating tools, is associated with a spectrum of neurovascular symptoms. To date, only its vascular pathology has been extensively studied; we sought to determine what direct neurologic injury, if any, is caused by vibration. Hindlimbs of anesthetiz...

    journal_title:Microsurgery

    pub_type: 杂志文章

    doi:10.1002/micr.20081

    authors: Matloub HS,Yan JG,Kolachalam RB,Zhang LL,Sanger JR,Riley DA

    更新日期:2005-01-01 00:00:00