On sampling and sampling errors in histomorphometry of peripheral nerve fibers.

Abstract:

:Histomorphometrical assessment of regenerated peripheral nerves is a very common goal of many studies in experimental microsurgery. In this paper, the main critical issues in nerve fiber sampling for quantitative morphological assessment are addressed. The equal opportunity rule, i.e., the basic paradigm of random sampling, is described, together with an explanation of how sampling errors, in the selection of histologic fields and of the nerve fibers inside them, can produce a bias in quantitative estimates. Finally, some practical suggestions on how to cope with the most common sampling errors are provided, in order to help researchers obtain reliable histomorphometrical data on peripheral nerve fibers.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Geuna S,Gigo-Benato D,Rodrigues Ade C

doi

10.1002/micr.10199

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

72-6

issue

1

eissn

0738-1085

issn

1098-2752

journal_volume

24

pub_type

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