Long-term evaluation of nerve regeneration in a biodegradable nerve guide.

Abstract:

:Nerve regeneration using artificial biodegradable conduits is of increasing interest. The aim of this study is to evaluate the regeneration and maturation of a nerve after long-term implantation (2 years) of a biodegradable poly-L-lactide/poly-epsilon-caprolactone (PLLA/PCL) copolymeric nerve guide in the sciatic nerve of the rat. After harvesting, we evaluated both the regenerated nerves and the controls, using light microscopy, transmission electron microscopy, and morphometric techniques. Remnants of biomaterial were still present after 2 years of implantation, but the foreign body reaction was very mild at this stage, due to the rounded shapes of the polymer debris. Morphometric analysis showed significant differences between the regenerated nerve and the normal sciatic nerve: the number of myelinated fibers is higher, and the mean fiber diameter of the myelinated fibers in the regenerated nerve is smaller. In conclusion, the results demonstrate that the new PLLA/PCL nerve guide can provide optimal conditions for regeneration and maturation of damaged nerves.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Den Dunnen WF,Van der Lei B,Schakenraad JM,Blaauw EH,Stokroos I,Pennings AJ,Robinson PH

doi

10.1002/micr.1920140808

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

508-15

issue

8

eissn

0738-1085

issn

1098-2752

journal_volume

14

pub_type

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