Oxidative stress causes ferredoxin-NADP+ reductase solubilization from the thylakoid membranes in methyl viologen-treated plants.

Abstract:

:The flavoenzyme ferredoxin-NADP+ reductase (FNR) is a member of the cellular defense barrier against oxidative damage in Escherichia coli. We evaluated the responses of chloroplast FNR to methyl viologen, a superoxide radical propagator, in wheat (Triticum aestivum L.) plants and chloroplasts. Treatments with the herbicide showed little effect on the levels of FNR protein or transcripts, indicating that expression of this reductase is not upregulated by oxidants in plants. Viologens and peroxides caused solubilization of active FNR from the thylakoids into the stroma, converting the enzyme from a membrane-bound NADPH producer to a soluble NADPH consumer. This response appeared specific for FNR, since other thylakoid proteins were unaffected by the treatments. The reductase-binding protein was released together with FNR, suggesting that it might be the target of oxidative modification. Stromal accumulation of a functional NADPH reductase in response to oxidative stress is formally analogous to the induction of FNR synthesis observed in E. coli under similar conditions. FNR solubilization may be playing a crucial role in maintaining the NADPH/NADP+ homeostasis of the stressed plastid. The unchecked accumulation of NADPH might otherwise increase the risks of oxidative damage through a rise in the Mehler reaction rates and/or the production of hydroxyl radicals.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Palatnik JF,Valle EM,Carrillo N

doi

10.1104/pp.115.4.1721

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

1721-7

issue

4

eissn

0032-0889

issn

1532-2548

pii

115/4/1721

journal_volume

115

pub_type

杂志文章
  • Competitive Inhibition of Abscisic Acid-Regulated Gene Expression by Stereoisomeric Acetylenic Analogs of Abscisic Acid.

    abstract::The properties of two enantiomeric synthetic acetylenic abscisic acid (ABA) analogs (PBI-51 and PBI-63) in relation to ABA-sensitive gene expression are reported. Using microspore-derived embryos of Brassica napus as the biological material and their responsiveness to ABA in the expression of genes encoding storage pr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.469

    authors: Wilen RW,Hays DB,Mandel RM,Abrams SR,Moloney MM

    更新日期:1993-02-01 00:00:00

  • Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes.

    abstract::To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would he...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00155

    authors: Verkest A,Byzova M,Martens C,Willems P,Verwulgen T,Slabbinck B,Rombaut D,Van de Velde J,Vandepoele K,Standaert E,Peeters M,Van Lijsebettens M,Van Breusegem F,De Block M

    更新日期:2015-08-01 00:00:00

  • Starch-branching enzyme I-deficient mutation specifically affects the structure and properties of starch in rice endosperm.

    abstract::We have isolated a starch mutant that was deficient in starch-branching enzyme I (BEI) from the endosperm mutant stocks of rice (Oryza sativa) induced by the treatment of fertilized egg cells with N-methyl-N-nitrosourea. The deficiency of BEI in this mutant was controlled by a single recessive gene, tentatively design...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.021527

    authors: Satoh H,Nishi A,Yamashita K,Takemoto Y,Tanaka Y,Hosaka Y,Sakurai A,Fujita N,Nakamura Y

    更新日期:2003-11-01 00:00:00

  • Photoinactivation of Detergent-Solubilized Plasma Membrane ATPase from Rosa damascena: Action Spectra.

    abstract::The photochemistry of vesicular and detergent-solubilized preparations of plasma membrane-associated ATPase was investigated in Rosa damascena. The cholate-solubilized ATPase activity fractionated into two peaks on a Sephadex G-150 column with simple, but different ultraviolet (UV) sensitivities. The larger enzyme was...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.3.617

    authors: Imbrie CW,Murphy TM

    更新日期:1984-03-01 00:00:00

  • Influence of Anaerobiosis on Chlorophyll Biosynthesis in Greening Oat Seedlings (Avena sativa L.).

    abstract::The influence of anaerobiosis for 0.5 to 15 hours on the last steps of chlorophyll biosynthesis of etiolated oat seedlings was investigated. Phototransformation of protochlorophyllide to chlorophyllide is only slightly reduced and esterification of chlorophyllide is slightly increased by pretreatment under anaerobic c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.4.576

    authors: Schoch S,Hehlein C,Rüdiger W

    更新日期:1980-10-01 00:00:00

  • Oxygen Uptake and Photosynthesis of the Red Macroalga, Chondrus crispus, in Seawater: Effects of Light and CO(2) Concentration.

    abstract::With an experimental system using mass spectrometry techniques and infra-red gas analysis of CO(2) developed for aquatic plants, we studied the responses to various light intensities and CO(2) concentrations of photosynthesis and O(2) uptake of the red macroalga Chondrus crispus S. The CO(2) exchange resistance at air...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.4.919

    authors: Brechignac F,Andre M

    更新日期:1984-08-01 00:00:00

  • Phase shift of the circadian rhythm of lemna caused by pulses of a leucine analog, trifluoroleucine.

    abstract::Pulses of a fluorinated analog of leucine, 5',5',5'-trifluoroleucine, reset the phase of the circadian rhythm of K(+) uptake in Lemna gibba G3 under continuous light conditions. The trifluoroleucine pulse caused the largest delay phase-shifts during the early subjective phase but it caused only small phase advances. T...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.953

    authors: Kondo T

    更新日期:1988-11-01 00:00:00

  • Auxin requirements of sycamore cells in suspension culture.

    abstract::Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this ;auxin requirement,' it has been shown by gas ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.4.927

    authors: Moloney MM,Hall JF,Robinson GM,Elliott MC

    更新日期:1983-04-01 00:00:00

  • Studies on Cation-induced Thylakoid Membrane Stacking, Fluorescence Yield, and Photochemical Efficiency.

    abstract::Trypsin digestion of photosynthetic membranes isolated from spinach (Spinacia oleracea L.) leaves eliminates the cation stimulation of chlorophyll fluorescence. High concentrations of cations protect the fluorescence yield against trypsin digestion, and the cation specificity for this protection closely resembles that...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.6.879

    authors: Jennings RC,Forti G,Gerola PD,Garlaschi FM

    更新日期:1978-12-01 00:00:00

  • Regulation of ribulose-1,5-bisphosphate carboxylase expression in second leaves of maize seedlings from low and high yield populations.

    abstract::Ribulose-1,5-bisphosphate carboxylase oxygenase (EC 4.1.1.39) (Rubisco) activity, Rubisco-protein, and Rubisco large and small subunit gene (rbcL and rbcS) transcripts were measured at seven stages of development in the second leaf of maize (Zea mays L.) seedlings belonging to low and high yield populations. During th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.541

    authors: Loza-Tavera H,Martínez-Barajas E,Sánchez-de-Jiménez E

    更新日期:1990-06-01 00:00:00

  • Activation of Chloroplast NADP-linked Glyceraldehyde-3-Phosphate Dehydrogenase by the Ferredoxin/Thioredoxin System.

    abstract::NADP-glyceraldehyde-3-P dehydrogenase of spinach (Spinacia oleracea) chloroplasts was activated by thioredoxin that was reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol. The activation process that was observed with the soluble protein fraction from ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.669

    authors: Wolosiuk RA,Buchanan BB

    更新日期:1978-04-01 00:00:00

  • Tonoplast stability and survival of isolated vacuoles in different buffers.

    abstract::The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. Howeve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.2.251

    authors: De Leon JL,Daie J,Wyse R

    更新日期:1988-10-01 00:00:00

  • Callose induction in cowpea by uridine diphosphate glucose and calcium phosphate-boric Acid treatments.

    abstract::Calcium phosphate-boric acid treatments and UDP-glucose both elicited aniline blue fluorescent, periodic acid-Schiff's reagent-resistant, deposits in association with the cell walls of cowpea (Vigna sinensis [Torner] Savi cv. Early Ramshorn) tissue. Those deposits induced by calcium phosphateboric acid treatment ultra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.2.366

    authors: Tighe DM,Heath MC

    更新日期:1982-02-01 00:00:00

  • A Starch Deficient Mutant of Arabidopsis thaliana with Low ADPglucose Pyrophosphorylase Activity Lacks One of the Two Subunits of the Enzyme.

    abstract::A starch deficient mutant of Arabidopsis thaliana (L.) Heynh. has been isolated in which leaf extracts contain only about 5% as much activity of ADPglucose pyrophosphorylase (EC 2.7.7.27) as the wild type. A single, nuclear mutation at a previously undescribed locus designated adg2 is responsible for the mutant phenot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.1175

    authors: Lin TP,Caspar T,Somerville CR,Preiss J

    更新日期:1988-12-01 00:00:00

  • Inhibition of early steps in the gibberellin-activated synthesis of alpha-amylase.

    abstract::Subsequent production of amylase is severely inhibited if barban [4-chloro-2-butynyl N-(3-chlorophenyl) carbamate] is added to embryo-free half seeds of barley within 4 to 5 hours after gibberellic acid treatment of these seeds. Thirty to 50 mg/L concentrations of barban are effective. Barban inhibition is non-competi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.2.195

    authors: Yung KH,Mann JD

    更新日期:1967-02-01 00:00:00

  • Electrical Potentials during Gravitropism in Bean Epicotyls.

    abstract::An apparatus for measuring simultaneously the surface electrical potentials along epicotyl was developed to study the largely bending situation by gravity. Potentials increased on the upper side and decreased on the lower side after the horizontal placement. The time course of electrical changes consisted of two compo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.1.193

    authors: Imagawa K,Toko K,Ezaki S,Hayashi K,Yamafuji K

    更新日期:1991-09-01 00:00:00

  • Partitioning of Intermediary Carbon Metabolism in Vesicular-Arbuscular Mycorrhizal Leek.

    abstract::Vesicular-arbuscular mycorrhizal fungi are symbionts for a large variety of crop plants; however, the form in which they take up carbon from the host is not established. To trace the course of carbon metabolism, we have used nuclear magnetic resonance spectroscopy with [13C]glucose labeling in vivo and in extracts to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.1.7

    authors: Shachar-Hill Y,Pfeffer PE,Douds D,Osman SF,Doner LW,Ratcliffe RG

    更新日期:1995-05-01 00:00:00

  • Purification, characterization, and structural analysis of a plant low-temperature-induced protein.

    abstract::We have purified to near homogeneity a recombinant form of the protein BN28 (rBN28), expressed in response to low temperature in Brassica napus plants, and we have determined its solution structure. Antibodies raised against rBN28 were used to characterize the recombinant and native proteins. Similar to many other low...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.2.367

    authors: Boothe JG,Sönnichsen FD,de Beus MD,Johnson-Flanagan AM

    更新日期:1997-02-01 00:00:00

  • Patatin-related phospholipase pPLAIIIδ increases seed oil content with long-chain fatty acids in Arabidopsis.

    abstract::The release of fatty acids from membrane lipids has been implicated in various metabolic and physiological processes, but in many cases, the enzymes involved and their functions in plants remain unclear. Patatin-related phospholipase As (pPLAs) constitute a major family of acyl-hydrolyzing enzymes in plants. Here, we ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.216994

    authors: Li M,Bahn SC,Fan C,Li J,Phan T,Ortiz M,Roth MR,Welti R,Jaworski J,Wang X

    更新日期:2013-05-01 00:00:00

  • Effect of heat shock on the metabolism of glutathione in maize roots.

    abstract::High performance liquid chromatography analyses revealed that glutathione (GSH) and cysteine are two of the major low molecular weight thiol compounds in maize root extracts. Treatment of maize roots to heat shock temperatures of 40 degrees C resulted in a decrease of cysteine levels and an increase of GSH levels. Pul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.4.1031

    authors: Nieto-Sotelo J,Ho TH

    更新日期:1986-12-01 00:00:00

  • Interactions of light and a temperature shift on seed germination.

    abstract::Germination of Rumex obtusifolius L. seeds is potentiated to an observable degree in 2 minutes by a single shift in temperature from 20 to 35 C. Half-maximal potentiation requires less than 32 minutes at the higher temperature. Similar sensitivities to shifts in temperature were observed for seeds of Barbarea vulgaris...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.2.127

    authors: Taylorson RB,Hendricks SB

    更新日期:1972-02-01 00:00:00

  • RNA interference suppression of genes in glycosyl transferase families 43 and 47 in wheat starchy endosperm causes large decreases in arabinoxylan content.

    abstract::The cell walls of wheat (Triticum aestivum) starchy endosperm are dominated by arabinoxylan (AX), accounting for 65% to 70% of the polysaccharide content. Genes within two glycosyl transferase (GT) families, GT43 (IRREGULAR XYLEM9 [IRX9] and IRX14) and GT47 (IRX10), have previously been shown to be involved in the syn...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.222653

    authors: Lovegrove A,Wilkinson MD,Freeman J,Pellny TK,Tosi P,Saulnier L,Shewry PR,Mitchell RA

    更新日期:2013-09-01 00:00:00

  • Interactions between potassium and calcium in their absorption by intact barley plants. I. Effects of potassium on calcium absorption.

    abstract::Increasing concentrations of K (20, 200, 2000 mum) in the nutrient solution depressed Ca content and concentration in barley plants growing in nutrient solutions of low Ca concentrations (250 and 2500 mum). Increasing K from 20 to 200 mum depressed Ca absorption more than increasing K from 200 to 2000 mum K.The strong...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.10.1717

    authors: Johansen C,Edwards DG,Loneragan JF

    更新日期:1968-10-01 00:00:00

  • Nod factors induce nod factor cleaving enzymes in pea roots. Genetic and pharmacological approaches indicate different activation mechanisms.

    abstract::Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from S...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.061705

    authors: Ovtsyna AO,Dolgikh EA,Kilanova AS,Tsyganov VE,Borisov AY,Tikhonovich IA,Staehelin C

    更新日期:2005-10-01 00:00:00

  • Abundant accumulation of the calcium-binding molecular chaperone calreticulin in specific floral tissues of Arabidopsis thaliana.

    abstract::Calreticulin (CRT) is a calcium-binding protein in the endoplasmic reticulum (ER) with an established role as a molecular chaper-one. An additional function in signal transduction, specifically in calcium distribution, is suggested but not proven. We have analyzed the expression pattern of Arabidopsis thaliana CRTs fo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.1.29

    authors: Nelson DE,Glaunsinger B,Bohnert HJ

    更新日期:1997-05-01 00:00:00

  • Light-dependent modifications in the metabolic responses of squash seedlings.

    abstract::Light-induced modifications in lipoxidase metabolism and chlorophyll formation in the cotyledon of squash (Cucurbita moscata) seedlings were determined. The enzyme activity decreased as light intensity increased, but chlorophyll continued to accumulate long after lipoxidase activity had virtually disappeared. Consider...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.5.780

    authors: Surrey K,Barr EM

    更新日期:1966-05-01 00:00:00

  • Rapid Accumulation of Anionic Peroxidases and Phenolic Polymers in Soybean Cotyledon Tissues following Treatment with Phytophthora megasperma f. sp. Glycinea Wall Glucan.

    abstract::Phytophthora megasperma Drechs. f. sp. glycinea Kuan & Erwin (PMG) cell wall glucan has been extensively characterized as an elicitor of the pterocarpan phytoalexins, the glyceollins in soybean (Glycine max L.). Just recently, this glucan was shown to be a potent elicitor of conjugates of the isoflavones, daidzein and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.4.1445

    authors: Graham MY,Graham TL

    更新日期:1991-12-01 00:00:00

  • Reductions of Rubisco activase by antisense RNA in the C4 plant Flaveria bidentis reduces Rubisco carbamylation and leaf photosynthesis.

    abstract::To function, the catalytic sites of Rubisco (EC 4.1.1.39) need to be activated by the reversible carbamylation of a lysine residue within the sites followed by rapid binding of magnesium. The activation of Rubisco in vivo requires the presence of the regulatory protein Rubisco activase. This enzyme is thought to aid t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056077

    authors: von Caemmerer S,Hendrickson L,Quinn V,Vella N,Millgate AG,Furbank RT

    更新日期:2005-02-01 00:00:00

  • Abscisic acid regulates axillary bud outgrowth responses to the ratio of red to far-red light.

    abstract::Low red light/far-red light ratio (R:FR) serves as an indicator of impending competition and has been demonstrated to suppress branch development. The regulation of Arabidopsis (Arabidopsis thaliana) rosette bud outgrowth by the R:FR and the associated mechanisms were investigated at several levels. Growth under low R...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221895

    authors: Reddy SK,Holalu SV,Casal JJ,Finlayson SA

    更新日期:2013-10-01 00:00:00

  • Tailoring the specificity of a plant cystatin toward herbivorous insect digestive cysteine proteases by single mutations at positively selected amino acid sites.

    abstract::Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.115741

    authors: Goulet MC,Dallaire C,Vaillancourt LP,Khalf M,Badri AM,Preradov A,Duceppe MO,Goulet C,Cloutier C,Michaud D

    更新日期:2008-03-01 00:00:00