Abstract:
:Formalin-fixed paraffin-embedded (FFPE) tissues are a real treasure for retrospective analysis considering the amount of samples present in hospital archives, combined with pathological, clinical, and outcome information available for every sample. Although unlocking the proteome of these tissues is still a challenge, new approaches are being developed. In this review, we summarize the different mass spectrometry platforms that are used in human clinical studies to unravel the FFPE proteome. The different ways of extracting crosslinked proteins and the analytical strategies are pointed out. Also, the pitfalls and challenges concerning the quality of FFPE proteomic approaches are depicted. We also evaluated the potential of these analytical methods for future clinical FFPE proteomics applications.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Maes E,Broeckx V,Mertens I,Sagaert X,Prenen H,Landuyt B,Schoofs Ldoi
10.1007/s00726-013-1494-0subject
Has Abstractpub_date
2013-08-01 00:00:00pages
205-18issue
2eissn
0939-4451issn
1438-2199journal_volume
45pub_type
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