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A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers.

Abstract：

OBJECTIVES:The aim of this study is to assess chromosomal damage in Tunisian hospital workers occupationally exposed to low levels of ionizing radiation (IR). MATERIALS AND METHODS:The cytokinesis-block micronucleus (CBMN) assay in the peripheral lymphocytes of 67 exposed workers compared to 43 controls matched for gender, age and smoking habits was used. The clastogenic/aneugenic effect of IR was evaluated using the CBMN assay in combination with fluorescence in situ hybridization with human pan-centromeric DNA in all the exposed subjects and controls. RESULTS:The study showed a significant increase of the micronucleus (MN) frequency in the lymphocytes of the exposed workers compared to the control group (13.63 ± 4.9‰ vs. 6.52 ± 4.21‰, p < 0.05). The centromere analysis performed in our study showed that MNs in hospital staff were predominantly centromere negative (72%) and the mean negative labeled micronucleus (C-MN) frequency was significantly higher in the exposed subjects than in the controls (9.04 ± 4.57‰ vs. 1.17 ± 0.77‰). The multivariate regression analysis, taking into account all confounding factors, showed that only the time of exposure to IR had a significant effect on the level of MNs and C-MN. CONCLUSION:The present study shows that chromosomal damage leading to the formation of micronucleated lymphocytes is more frequent in the hospital workers exposed to IR than in the controls, despite the low levels of exposure. The results of the study confirm the well-known clastogenic properties of ionizing radiation. In regards to health monitoring, detection of early genotoxic effects may allow for the adoption of preventive biological control measures, such as hygienic improvements in the workplace or reduction of hours of occupational exposure.

journal_name

Int J Occup Med Environ Health

journal_title

International journal of occupational medicine and environmental health

authors

Bouraoui S,Mougou S,Drira A,Tabka F,Bouali N,Mrizek N,Elghezal H,Saad A

doi

10.2478/s13382-013-0084-4

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

144-54

issue

eissn

1232-1087

issn

1896-494X

journal_volume

pub_type

杂志文章,多中心研究

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A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed Tunisian hospital workers.