Abstract：
OBJECTIVE:This study assesses the antitubercular potential of natural products obtained from plants reputed to have medicinal properties and collected from the tropical flora of Puerto Rico. BACKGROUND:The increase in persons infected with Mycobacterium tuberculosis (MTB) the world over and the development of resistance to antibiotics by this microbe and other infectious bacteria has created the need for new drugs to replace those which have lost effectiveness. METHOD:In Phase I of this study, ethanolic leaf extracts of fifty local plants were submitted to preliminary screening to assess their in vitro Mycobacterium smegmatis inhibitory activity using the Bauer-Kirby disk diffusion method. In Phase II, the definitive screening of the six most promising extracts which inhibited M. smegmatis were assayed for their MTB inhibitory activity using the BACTEC 460 susceptibility test method. The brine shrimp bioassay was used as a toxicity bioassay and the mice inoculation test was used to determine mice tolerance to the effect of the daily intraperitoneal inoculations of the plant extracts. RESULTS:MTB showed varying degrees of susceptibility to each plant extract. This effect was dependent upon the plant species, dose and time of exposure. Evidence is provided suggesting that: (1) Six crude plant extracts (12%) tested possessed inhibitory capacity at the amount of 500 micrograms per disc; (2) Mammea americana extract yielded the strongest inhibitory effect at 50 micrograms per disc, followed by Marchantia polymorpha, Mangifera indica, Callistemon citrinus, Syzygium jambos and Momordica charantia; (3) the bactericidal inhibitory pattern of MTB growth, exposed to Mammea americana extract, was comparable to streptomycin; and (4) the transitory reduction pattern of MTB growth, produced by Callistemon citrinus, Marchantia polymorpha extracts at 100 micrograms and 250 micrograms, was similar to that of bacteriostatic agents. CONCLUSION:Of 50 plants screened six extracts tested for their anti-MTB activity yielded positive results with varying degrees of inhibition. Mammea americana showed the greatest inhibitory activity suggesting that certain plant species yield valuable anti-Mycobacterium tuberculosis substances. The procedures employed in this study, including the BACTEC 460 modified method, are useful for in vitro screening of plant extracts with potential antitubercular activity.
journal_name
P R Health Sci Jjournal_title
Puerto Rico health sciences journalauthors
Frame AD,Ríos-Olivares E,De Jesús L,Ortiz D,Pagán J,Méndez Ssubject
Has Abstractpub_date
1998-09-01 00:00:00pages
243-52issue
3eissn
0738-0658issn
2373-6011journal_volume
17pub_type
杂志文章abstract：:Small island exigencies and a legacy of colonial jurisprudence set the stage for this three-year study in 2001-2003 of abortion practice on several islands of the northeast Caribbean: St. Martin, St. Maarten, Anguilla, Antigua and St Kitts. Based on in-depth interviews with 26 physicians, 16 of whom were performing ab...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：OBJECTIVE:This study intends to determine the prevalence of multidrug resistant (MDR) infections by A. baumannii, K. pneumoniae and P. aeruginosa in a tertiary care teaching hospital intensive care unit (ICU) in San Juan, PR, estimate the mortality rate and compare the morbidity and mortality differences among those tr...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:Left ventricular (LV) function parameters have major diagnostic and prognostic importance in heart disease. Measurement of ventricular function with tomographic (SPECT) radionuclide ventriculography (MUGA) decreases camera time, improves contrast resolution, accuracy of interpretation and the overall reliabil...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:Breastfeeding has experienced a surge in Puerto Rico thanks to the efforts of many women, as well as governmental, non-governmental and private organizations. Among the aspects which can affect the initiation, establishment and maintenance of successful breastfeeding one finds potential ethical conflicts among health ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:The identification of Streptococcus mutans from dental plaque samples with rapid systems, such as the RapID STR System, has not been recommended by the manufacturers. In this study, we evaluated the use of the above method for the identification of 34 human dental plaque Streptococcus mutans strains which had been ide...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:This article examines the available data on the health status of Puerto Rican women in the United States. Statistics on reproductive health, cancer and AIDS indicate that the health status of these women is lower than that of both their U.S. and island counterparts. Usually, the health of immigrants lies somewhere bet...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：OBJECTIVE:To evaluate the utility of the substituted judgement standard in terminally ill patients by determining agreement between patients, family proxies and physicians. BACKGROUND:Several studies have addressed the utility of substituted judgement, showing conflicting data whether surrogates are accurate predictin...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：OBJECTIVE:The prevalence of inflammatory bowel disease [IBD, Crohn's disease (CD) and ulcerative colitis (UC)] in Puerto Rico has increased significantly in recent years. Experience with these diseases outside of a referral center is limited. A registry for IBD, created by the University of Puerto Rico (UPR) Center for...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：:Although injection drug use (IDU) is the major risk category for HIV infection in Puerto Rico and injection drug users start their drug injection careers very young, there is little information related to drug injection patterns of young IDUs. In this paper we report on factors found to be associated with HIV seroposi...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：OBJECTIVE:To determine the IgE reactivity against recombinant protein Blo t 1 from the dust mite Blomia tropicalis (Bt) using serum from patients with positive skin test to this mite and to investigate the cross-reactivity between Bt and Dermatophagoides pteronyssinus. BACKGROUND:Dust mites have an important role as i...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:Thymidine kinase is a key enzyme responsible for the activation of several anticancer and antiviral drugs. As the first enzyme in the salvage pathway of thymidine, it is regulated by the feedback inhibition exerted by the end-product of the pathway, namely thymidine 5'-triphosphate. 5'-Aminothymidine is a non-toxic an...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：1994-03-01 00:00:00
abstract：OBJECTIVE:Shunt infection is the most common complication following a Cerebrospinal fluid (CSF) diversion procedure with devastating consequences. This study analyzes the efficacy of different shunt systems in reducing early shunt infections in the pediatric population. METHODS:Retrospective case study analysis of 177...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2019-12-01 00:00:00
abstract：OBJECTIVE:To determine the frequency of pediatric brain tumors treated at the University Pediatric Hospital of Puerto Rico over a 6-year period and examine the demographic data and tumor histology of patients in different age groups. METHODS:A retrospective study was undertaken at the University Pediatric Hospital. We...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:Genital human papillomavirus (HPV) infection is one of the most commonly diagnosed sexually transmitted infections world-wide. Over the last two decades, research has established a strong causal link between specific types of HPV infection and cancer, particularly cervical, anal, vulvar/vaginal, penile, and oropharyng...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：2010-09-01 00:00:00
abstract：:Atrial fibrillation (AF) is the most common sustained arrhythmia in adults. It is a significant public health problem in the United States where it affects 2.2 million Americans and almost 10% of the population older than 80 years. It should be emphasized that the prevalence of AF increases with advancing age and with...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：2004-12-01 00:00:00
abstract：:DNA microarray is a technology that simultaneously evaluates quantitative measurements for the expression of thousands of genes. DNA microarrays have been used to assess gene expression between groups of cells of different organs or different populations. In order to understand the role and function of the genes, one ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：2009-06-01 00:00:00
abstract：:Breastfeeding mothers need orientation and help in order to succeed with breastfeeding. Lack of support from health professionals and societal barriers result in a median duration of breastfeeding in Puerto Rico of only 3 weeks. A telephone warm line at our breastfeeding clinic tries to provide some much-needed suppor...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：INTRODUCTION:Incidental adrenal tumors are commonly benign, but reports demonstrate that if the characteristics of the tumor are not clear, on images surgery is the procedure of choice. Our objective through this case is to show that laparoscopic adrenalectomy is a safe approach for adrenal incidental tumor regardless ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：OBJECTIVE:Colorectal cancer (CRC) is among the most common cancers in Puerto Rico. Few studies have correlated clinical and pathological variables with the overall survival of CRC patients in Puerto Rico. We report the clinical and pathological characteristics of patients who underwent surgical resection at a community...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2014-06-01 00:00:00
abstract：:The World Health Organization criteria for the diagnosis of Osteopenia and Osteopoposis was applied to a control group of 33 females ages 50 to 59 years and 24 females ages 60 to 69 years. The general exclusion criteria for the selection of subjects included early menopause and diseases, use of drugs and toxic habits ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：OBJECTIVE:This study aimed to assess the role of gender on HIV/AIDS stigma among 507 medical students in Puerto Rico. METHODS:A secondary data analysis was performed with baseline measurements of a controlled randomized study. RESULTS:Unadjusted analyses showed that the overall multivariate test for gender was signif...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:To examine the relationship of the symptoms of diarrhea and fatigue by testing a model that included multiple dimensions of the cancer related-symptom experience. METHODS:A secondary data analysis was conducted on data from the self-reports of 102 cancer patients co experiencing diarrhea and fatigue during t...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:Due to advances in the care of people living with HIV/AIDS (PLWHA), life expectancy significantly increased, putting this group vulnerable to age-related comorbidities, such as cancer. The objective of this study was to describe the knowledge of cancer screening (cervical, breast, anal, colon, prostate) and o...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:This study was conducted to determine the experience of tooth loss, as well as its associated variables, in a sample of adult Mexicans, aged 60 years and older. MATERIALS AND METHODS:This cross-sectional study was part of a larger project to measure diverse oral health indicators in a convenience sample: it ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2016-06-01 00:00:00
abstract：:113 rhesus monkeys, representing 4 age classes, 3 matrilines, and immigrant adult males in a 161-member Cayo Santiago-derived troop living in a 2-acre enclosure, were sampled for levels of plasma ACTH and cortisol during a period of capture and brief cage confinement for routine veterinary examination. ACTH levels sho...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:The AIDS pandemic had a significant impact in Puerto Rico, especially among the heterosexual populations, in particular women. Women are one of the fastest growing risk groups with HIV/AIDS in the USA and constitute about half of the AIDS cases in the world. During the past 10 years Puerto Rico has ranked among the to...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2007-12-01 00:00:00
abstract：:The therapy of chronic myeloid leukemia (CML), characterized by the presence of the Philadelphia chromosome in the clonal hematopoietic stem cells, has changed dramatically in the last year with the development of a specific inhibitor of the BCR-ABL tyrosine kinase. This medication, STI571 or imatinib, was recently ap...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：2002-09-01 00:00:00
abstract：OBJECTIVE:Dental caries is one of the most prevalent chronic diseases in children. Currently, no data are available on dental caries prevalence in the Dominican Republic. The purpose of this study was to estimate the prevalence of dental caries in schoolattending 12-year-olds in San Pedro de Macorís, Dominican Republic...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2020-06-01 00:00:00
abstract：:In studying the process of protein synthesis of a silk-producing organism we have found that several macromolecules must be synthesized in order for the process to occur. Through time course studies, we have found that small RNAs may play a paramount role in directing the finely orchestrated process. Alanine tRNA, U1 ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：OBJECTIVE:To evaluate and compare the efficacy in obtaining an adequate endocervical sampling using the endocervical brush and the endocervical curettage. METHODS:Analysis of the cytology and histology of samples obtained from patients referred to the University of Puerto Rico School of Medicine Tertiary Care Center A...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
