Protein-ligand interactions: interaction of nitrosamines with nicotinic acetylcholine receptor.

abstract：:Pulmonary fibrosis is a progressive disorder whose molecular pathology is poorly understood. Here we developed an in-house cDNA microarray ("lung chip") originating from a lung-normalized cDNA library. By using this lung chip, we analyzed global gene expression in a murine model of bleomycin-induced fibrosis and selec...

journal_title：Biochemical and biophysical research communications

authors： Katsuma S,Nishi K,Tanigawara K,Ikawa H,Shiojima S,Takagaki K,Kaminishi Y,Suzuki Y,Hirasawa A,Ohgi T,Yano J,Murakami Y,Tsujimoto G

更新日期：2001-11-09 00:00:00

abstract：:Receptor tyrosine kinase EphA7 is specifically expressed in otic region in Xenopus early development. However, its role in otocyst development remains unknown. Knockdown of EphA7 by a specific morpholino oligonucleotide (MO) reduced the size of the otocyst and triggered otic epithelial cell extrusion. Interestingly, E...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Sun J,Wang Z,Li C,Mao B

更新日期：2020-05-28 00:00:00

abstract：:Rheumatoid arthritis (RA) is a systemic, chronic inflammatory disease that is characterized by T helper 17 (Th17) cell- and osteoclast-induced joint destruction and inflammation. In RA, several cytokines (interleukin (IL)-1, 6,17, and tumor necrosis factor (TNF)) are involved in almost all aspects of articular inflamm...

journal_title：Biochemical and biophysical research communications

authors： Park YH,Kim HJ,Lee K,Choi Y,Heo TH

更新日期：2020-02-19 00:00:00

abstract：:Previous studies in our laboratory showed that ANP inhibits increases in endothelial monolayer permeability to macromolecules induced by thrombin. In this present study, we investigated the second messenger system involved in the influence of ANP on monolayer permeability. In bovine aortic endothelial cells (BAEC), AN...

journal_title：Biochemical and biophysical research communications

authors： Lofton CE,Newman WH,Currie MG

更新日期：1990-10-30 00:00:00

abstract：:1-4 interaction scaling factors are used in AMBER forcefields to reduce the exaggeration of short-range repulsion caused by the 6-12 Lennard-Jones potential and a nonpolarizable charge model and to obtain better agreements of small-molecule conformational energies with experimental data. However, the effects of these ...

journal_title：Biochemical and biophysical research communications

authors： Pang YP

更新日期：2015-02-06 00:00:00

abstract：:In this study, we investigated the IL-1 beta converting enzyme (ICE) family cysteine proteases responsible for the Fas-mediated apoptosis of rheumatoid arthritis (RA) synoviocytes and their involvement in proinflammatory cytokine production. CPP32 inhibitor, but not ICE inhibitor, was capable of inhibiting the Fas-med...

journal_title：Biochemical and biophysical research communications

authors： Sekine C,Yagita H,Kobata T,Hasunuma T,Nishioka K,Okumura K

更新日期：1996-11-01 00:00:00

abstract：:Thyrotropin receptor (TSHR) in extrathyroidal tissue, especially fat tissue, is supposed to have important roles in the development of extrathyroidal manifestations of Graves' disease. However, the molecular mechanism of TSHR expression is not known. Expression of TSHR mRNA and TSH-dependent cAMP production were obser...

journal_title：Biochemical and biophysical research communications

authors： Haraguchi K,Shimura H,Lin L,Saito T,Endo T,Onaya T

更新日期：1996-06-05 00:00:00

abstract：:Tissue factor (TF) is an initiation factor for blood coagulation and its expression is induced on endothelial cells during inflammatory or immune responses. We designed an antisense oligodeoxynucleotide (AS-1/TF) for rat TF and studied its effect on hepatic ischemic reperfusion injury. AS-1/TF was delivered intravenou...

journal_title：Biochemical and biophysical research communications

authors： Nakamura K,Kadotani Y,Ushigome H,Akioka K,Okamoto M,Ohmori Y,Yaoi T,Fushiki S,Yoshimura R,Yoshimura N

更新日期：2002-09-27 00:00:00

abstract：:Breast cancer anti-estrogen resistance 3 (BCAR3) is an SH2-containing signal transducer and is implicated in tumorigenesis of breast cancer cells. In this study, we found that BCAR3 mediates the induction of ERK activation and DNA synthesis by insulin, but not by IGF-1. Specifically, the SH2 domain of BCAR3 is involve...

journal_title：Biochemical and biophysical research communications

authors： Oh MJ,Yi SJ,Kim HS,Kim JH,Jeong YH,van Agthoven T,Jhun BH

更新日期：2013-11-29 00:00:00

abstract：:Functional endothelial cells and their progenitors are required for vascular development, adequate vascular function, vascular repair and for cell-based therapies of ischemic diseases. Currently, cell therapy is limited by the low abundance of patient-derived cells and by the functional impairment of autologous endoth...

journal_title：Biochemical and biophysical research communications

authors： Kim J,Eligehausen S,Stehling M,Nikol S,Ko K,Waltenberger J,Klocke R

更新日期：2014-01-10 00:00:00

abstract：:Dehaloperoxidase (DHP) from Amphitrite ornata is the only heme-containing, hydrogen peroxide-dependent globin capable of oxidatively dehalogenating halophenols to yield the corresponding quinones. To ascertain that this enzymatic activity is intrinsic to DHP, we have cloned and expressed the enzyme in Escherichia coli...

journal_title：Biochemical and biophysical research communications

authors： Osborne RL,Taylor LO,Han KP,Ely B,Dawson JH

更新日期：2004-11-26 00:00:00

abstract：:Fibronectin, von Willebrand factor, and fibrinogen each bind to the glycoprotein IIb-IIIa complex on activated platelets via an arg-gly-asp-ser (RGDS) sequence present within the adhesive proteins. Both the IIb and IIIa polypeptides of the IIb-IIIa complex on thrombin activated platelets are specifically and extensive...

journal_title：Biochemical and biophysical research communications

authors： Santoro SA,Lawing WJ Jr

更新日期：1989-04-14 00:00:00

abstract：:Incubation of lipoxin A4 (LXA4) either with human leukocytes or with rat liver microsomes in the presence of NADPH very selectively led to a more polar metabolite retaining the conjugated tetraenic structure of LXA4. Lipoxin B4 (LXB4) underwent a very similar metabolism into a more polar metabolite, whereas the all-tr...

journal_title：Biochemical and biophysical research communications

authors： Boucher JL,Delaforge M,Mansuy D

更新日期：1991-05-31 00:00:00

abstract：:We have identified a novel reaction in which acetaldehyde promotes rat hepatic cytosolic catalysis of O2 consumption coupled with glutathione oxidation without apparent release of activated forms of O2. Acetaldehyde is not consumed in the reaction. The reaction (O2 consumption or oxidized glutathione production) is sa...

journal_title：Biochemical and biophysical research communications

authors： Lyon I,Kaplowitz N

更新日期：1985-06-28 00:00:00

abstract：:Plasma high (HDL) and low (LDL) density lipoproteins were isolated from rats fed a diet supplemented with either fish (menhaden) oil or hydrogenated coconut oil (control). Fluorescence polarization and electron spin resonance of labelled fatty acid probe molecules incorporated into the outer amphiphilic monolayer of H...

journal_title：Biochemical and biophysical research communications

authors： Belcher D,Stillwell W,Langsford CA,Wassall SR

更新日期：1988-02-15 00:00:00

abstract：:The serine proteinase catalyzed hydrolysis of N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p- nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) was investigated at pH 6.2 and 21.0 degrees C. The results are consistent with the minimum three-step catalytic mechanism. The acylation step is rate limiting for human...

journal_title：Biochemical and biophysical research communications

authors： Balliano G,Milla P,Giordano C,Gallina C,Coletta M,Menegatti E,Rizzi M,Bolognesi M,Ascenzi P

更新日期：1996-08-14 00:00:00

abstract：:The possible formation of a stable complex between extracellular signal-regulated kinases (ERKs) and Raf protein kinases was investigated in an attempt to understand the molecular mechanism by which Raf protein kinases were differentially regulated in response to the various stimuli, leading to the activation of ERKs ...

journal_title：Biochemical and biophysical research communications

authors： Kim SH,Yang YC

更新日期：1996-12-13 00:00:00

abstract：:Spiroplasma are wall-less bacteria which belong to the phylum Tenericutes that evolved from Firmicutes including Bacillus subtilis. Spiroplasma swim by a mechanism unrelated to widespread bacterial motilities, such as flagellar motility, and caused by helicity switching with kinks traveling along the helical cell body...

journal_title：Biochemical and biophysical research communications

authors： Takahashi D,Fujiwara I,Miyata M

更新日期：2020-12-17 00:00:00

abstract：:Adipogenesis is the process of differentiation from preadipocytes to adipocytes and is orchestrated by various transcription factors, such as the peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAAT-enhancer-binding protein alpha (C/EBPα). Oxidative stress is also a crucial factor in adipogenesis, an...

journal_title：Biochemical and biophysical research communications

authors： Kim BR,Lee GY,Yu H,Maeng HJ,Oh TJ,Kim KM,Moon JH,Lim S,Jang HC,Choi SH

更新日期：2018-03-18 00:00:00

abstract：:Cholesterol homeostasis is strictly maintained to prevent abnormal biological processes that arise from excessive accumulation of cholesterol in tissues. Although dyslipidemia causes reproductive dysfunction and endocrine disruption in male rats, regulatory factors and mechanisms have not been clearly demonstrated. Th...

journal_title：Biochemical and biophysical research communications

authors： Lim W,Bae H,Sohn JY,Jeong W,Kim SH,Song G

更新日期：2015-01-02 00:00:00

abstract：:Inducible nitric oxide (iNOS) is closely correlated with chronic inflammation in hepatitis B virus X protein (HBx)-induced hepatocellular carcinoma (HCC). However, the molecular mechanisms through which iNOS contribute to hepatocarcinogenesis remain poorly understood. Therefore, we investigated the role of iNOS in sig...

journal_title：Biochemical and biophysical research communications

authors： Park YH,Shin HJ,Kim SU,Kim JM,Kim JH,Bang DH,Chang KT,Kim BY,Yu DY

更新日期：2013-05-31 00:00:00

abstract：:Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and mu-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain acti...

journal_title：Biochemical and biophysical research communications

authors： Cuerrier D,Nie Z,Badley AD,Davies PL

更新日期：2005-02-04 00:00:00

abstract：:To explore the pathophysiologic roles of the obese (ob) gene product, leptin, in the development of obesity and hypertension, we examined ob gene expression and leptin secretion in obese spontaneously hypertensive rats (obese SHR or Koletsky rats) at the stage of established obesity and hypertension. Expression of the...

journal_title：Biochemical and biophysical research communications

authors： Hiraoka J,Hosoda K,Ogawa Y,Ikeda K,Nara Y,Masuzaki H,Takaya K,Nakagawa K,Mashimo T,Sawamura M,Koletsky RJ,Yamori Y,Nakao K

更新日期：1997-02-24 00:00:00

abstract：:Salmon calcitonin potently competes for amylin binding sites in rat brain and has amylin-like actions upon glucose metabolism in rat muscle. We report here that [125I]-salmon calcitonin binds to rat hindlimb muscle membranes with high affinity (Kd = 0.47 pM). Binding was inhibited by rat amylin (Ki = 2 nM), rat alpha ...

journal_title：Biochemical and biophysical research communications

authors： Kenney MA,Moore CX,Pittner R,Beaumont K

更新日期：1993-11-30 00:00:00

abstract：:Lipoteichoic acid is a major cell wall virulence factor of gram-positive bacteria. LTAs from various bacteria have differential immunostimulatory potentials due to heterogeneity in their structures. Although recent studies have demonstrated that LTA isolated from Lactobacillus plantarum (pLTA) has anti-inflammatory pr...

journal_title：Biochemical and biophysical research communications

authors： Jang KS,Baik JE,Han SH,Chung DK,Kim BG

更新日期：2011-04-22 00:00:00

abstract：:The kinetic parameters of several substrates of penicillin acylase from Streptomyces lavendulae have been determined. The enzyme hydrolyses phenoxymethyl penicillin (penicillin V) and other penicillins with aliphatic acyl-chains such as penicillin F, dihydroF, and K. The best substrate was penicillin K (octanoyl penic...

journal_title：Biochemical and biophysical research communications

authors： Torres-Guzmán R,de la Mata I,Torres-Bacete J,Arroyo M,Castillón MP,Acebal C

更新日期：2002-03-01 00:00:00

abstract：:The DNA-binding activity of a 42-kDa phosphoprotein from salivary gland cells and cultured epithelial cells of Chironomus tentans have been analyzed by the Southwestern technique. Both the salivary gland and the epithelial cell 42-kDa polypeptides were found to be single-stranded DNA-binding proteins. They bind to sin...

journal_title：Biochemical and biophysical research communications

authors： Stigare J,Egyhazi E

更新日期：1991-05-15 00:00:00

abstract：:Opening of high conductance permeability transition pores in mitochondria initiates onset of the mitochondrial permeability transition (MPT). The MPT is a causative event, leading to necrosis and apoptosis in hepatocytes after oxidative stress, Ca(2+) toxicity, and ischemia/reperfusion. CsA blocks opening of permeabil...

journal_title：Biochemical and biophysical research communications

authors： Kim JS,He L,Lemasters JJ

更新日期：2003-05-09 00:00:00

abstract：:Interleukin (IL)-19 belongs to the IL-10 family which includes IL-19, IL-20, IL-22, AK155, and MDA-7. IL-10 is a potent immunomodulatory cytokine with implications for pathogenesis in various autoimmune diseases. Polymorphism of the IL-10 promoter region correlates with disease outcome. To understand the gene regulati...

journal_title：Biochemical and biophysical research communications

authors： Chen PJ,Wei CC,Wang C,Chen FW,Hsu YH,Chang MS

更新日期：2006-06-09 00:00:00

abstract：:The cDNA for subunit d of rat mitochondrial H(+)-ATP synthase was cloned from a brain cDNA library. The protein contains internally repeat structures that have sequences similar to those of other ATP-related proteins. The antibodies raised against the protein A-subunit d fusion protein expressed in E. coli specificall...

journal_title：Biochemical and biophysical research communications

authors： Motojima K,Imanaka T

更新日期：1992-02-14 00:00:00