Abstract：
BACKGROUND/AIM:Cisplatin nephrotoxicity includes early activation of the pro-apoptotic p66Shc and disorganization of the actin cytoskeleton, integrity which is regulated by heat-shock protein-27 (Hsp27). Here we determined the potential role of p66Shc in abrogating the Hsp27 function. MATERIALS AND METHODS:Effects of p66Shc knockdown and Hsp27 overexpression on F-actin stress fibers after cisplatin treatment were visualized by phalloidin staining. Binding of p66Shc to Hsp27 after cisplatin treatment was determined by immunoprecipitation in cell and tissue lysates. The role of p66Shc and its Ser36 phosphorylation in Hsp27 binding was assessed by overexpressing it or mutating its Ser36 residue. RESULTS:Knockdown of p66Shc and overexpression of Hsp27 ameliorated cisplatin-mediated collapse of the actin cytoskeleton. Further studies revealed that p66Shc binds Hsp27 after treatment with cisplatin that requires Ser36 phosphorylation of p66Shc. CONCLUSION:We propose a novel function of p66Shc that, through interacting with Hsp27, accelerates cisplatin-dependent disruption of the actin cytoskeleton.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Arany I,Clark JS,Reed DK,Ember I,Juncos LAsubject
Has Abstractpub_date
2012-11-01 00:00:00pages
4759-63issue
11eissn
0250-7005issn
1791-7530pii
32/11/4759journal_volume
32pub_type
杂志文章abstract：:MOv18, a non-internalizing monoclonal antibody (MAb) with restricted tumor specificity, was conjugated to ricin toxin (RT). According to their ability to bind to galactose residues of Sepharose 6B, the immunoconjugates were fractionated into Bound and Unbound MOv18-RT. The two conjugates could be distinguished by SDS-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Mammalian cells transfected with the S. cerevisiae APN1 protein acquire resistance to oxidizing agents, the damage of which is mainly repaired via DNA base excision repair (BER). We have recently hypothesized that this effect might be linked to the possible capacity of APN1 to accelerate mammalian BER by its 3' dieste...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:The events during the settlement of BSp73AS (AS) tumor cells in the syngeneic rat lung are described. Although AS cells show highly invasive behavior in vitro, subcutaneous primary tumors grow solidly without detectable metastatic spreading. However, AS cells when applied to the syngeneic rats via the tail vein give r...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:Chemotherapy for patients with multiple myeloma was individually selected on the basis of antiproliferative drug sensitivity determined in vitro by a clonogenic assay. Myeloma stem cells were preincubated with various cytostatic drugs and allowed to form colonies in a plasma clot culture system. In 21 out of 24 prospe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：BACKGROUND:Signaling between androgen receptor (AR) and mTOR may be crucial for prostate cancer cells to endure the low androgen and suboptimal nutrient conditions produced by androgen deprivation therapy. MATERIALS AND METHODS:AR and mTOR cross-talk was examined in LNCaP cells exposed to either high or low testostero...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-10-01 00:00:00
abstract：:Four heart hormones, namely atrial natriuretic peptide (ANP), long-acting natriuretic peptide (LANP), vessel dilator and kaliuretic peptide reduce up to 97% of cancer cells in vitro. These four cardiac hormones eliminate up to 80% of human pancreatic adenocarcinomas, two-thirds of human breast carcinomas and up to 86%...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.11152
更新日期：2016-11-01 00:00:00
abstract：:We have established a practical system for generating antitumor effector lymphocytes using the tumor antigen peptide CEA and cultured dendritic cells (DCs), and have also characterized effector cells. DCs were induced from the adherent cell population of autologous peripheral blood mononuclear cells (PBMCs) obtained f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：BACKGROUND:It is essential that cytotoxic T-lymphocytes (CTLs) recognize tumor-associated antigens (TAAs) and exhibit antitumor effects in immunological responses for tumor rejection. However, only a few cases with oral squamous cell carcinoma (OSCC) have been studied for these antitumor mechanisms. We established a ca...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11898
更新日期：2017-09-01 00:00:00
abstract：BACKGROUND:Vinflunine is a novel Vinca alkaloid currently undergoing Phase II clinical trials, which have previously demonstrated anti-vascular effects in a transplantable murine colon adenocarcinoma model. Previous studies with compounds showing similar effects in combination with standard anti-cancer agents have demo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is the sixth most common malignancy in humans including type I diabetic and normal rats. Tobacco and alcohol, as well as dysregulation of oncogenes and tumor suppressor genes, epigenetic changes and mitochondrial mutations have been implicated in OSCC development. Recent epidemiolog...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2007-11-01 00:00:00
abstract：:The cell proliferation activity of sixteen childhood primitive neuroectodermal tumors (PNETs) was observed immunocytochemically, to determine the cell kinetics and cell proliferation activity of these relatively undifferentiated, malignant brain tumors. Two mouse anti-human monoclonal antibodies (MoABs) were employed ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:Basic conventional prognostic factors for breast cancer include the age of the patient, tumor grade, regional lymph nodes status, and estrogen (ER) and progesterone (PR) receptor status. Positivity of the HER2 receptor (c-erbB-2) seems to be a new prognostic and predictive factor. Prognostic factors seem to ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：:The M-VAC regimen (methotrexate, vinblastine, doxorubicin and cisplatin) is widely used in the treatment of advanced or metastatic bladder cancer. In the present trial, an alternate week regimen of M-VEC (with epidoxorubicin instead of doxorubicin) supported by G-CSF (filgrastim) was evaluated. Eligible patients had m...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:This study investigated whether neuraminidase (Neu) affects LFA-1 mRNA expression in spleen cells and whether somatostatin (SOM) and substance P (SP) treatment induce changes in the Neu mRNA expression level in spleen cells. Neu treatments down-regulated the LFA-1 mRNA levels after culturing for 2 h. SOM increased the...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：BACKGROUND:Recent developments in detection of micrometastasis have revealed a considerable incidence of systemic disease in patients who would previously have been diagnosed as having solid tumours only. The purpose of this study was to investigate the prevalence and clinical significance of circulating carcinoma cell...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：AIM:Combined cisplatin-topotecan therapy is standard care for advanced cervical cancer, however it is associated with haematotoxicity and nephrotoxicity. This trial was designed to assess the combination of carboplatin which is less nephrotoxic, and oral topotecan. PATIENTS AND METHODS:Patients with advanced/recurrent...
journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Intronic microRNAs (miRNAs) are considered to be transcribed using their host gene promoter. However, about one third of intronic miRNAs are predicted to have independent promoter elements. MATERIALS AND METHODS:Human breast cancer cells were cultured under normoxia or hypoxia, and expression levels of intr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11769
更新日期：2017-07-01 00:00:00
abstract：BACKGROUND/AIM:Gemcitabine is a drug commonly used to treat pancreatic cancer but chemoresistance to it is a common clinical issue. KML001 (sodium meta-arsenite) has demonstrated certain antitumor activity. The objective of the study was to evaluate the influence of KML001 on the anticancer activity of gemcitabine agai...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:The chromosomes were studied by an in vitro technique in four parametrial and one gastric leiomyomas. The findings in parametrial myomas (the present four cases and one published earlier) differed from the observations in uterine cases by the absence of normal stemlines and the absence of 1p,6p and 7q changes. As has ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:Vesnarinone (3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)- quinolinone), a quinolinone derivative, is an orally active inotropic agent used in Japan for the treatment of chronic heart failure. Recently, it has been reported that vesnarinone induces differentiation and apoptosis in certain types of leuk...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Thirty-two patients (30 men, 2 women), median age 68 years (range 47-85) with histologically confirmed advanced bladder carcinoma (Stages T3 and T4 according to the International Union Against Cancer staging system), who were poor surgical candidates, were prospectively treated with 1-6 (median 4) cycles of intra-arte...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:We have recently identified ICBP90 as being a protein able to bind in vitro a CCAAT box of the topoisomerase II alpha gene promoter. The aim of the present work was to check whether ICBP90 is able to regulate in vivo topoisomerase II alpha expression in human lung fibroblasts under various proliferating conditions. Tr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:A clearly benign parotid tumor, an adenolymphoma, was studied cytogenetically using short-term cultured material. The first preparation showed only a normal stemline. The two subsequent preparations were both characterized by a pseudodiploid stemline with the karyotype 46,XY,del(7) (p12p14-15), t(11;19) (q21;p12-13). ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：BACKGROUND/AIM:Non-vascularized methods to reconstruct bone defects following tumor resection are associated with complications. As a result, the free vascularized fibular flap (FVF) has become a mainstay for reconstruction. The purpose of this study was to analyze FVF reconstruction in the upper extremity. PATIENTS A...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14246
更新日期：2020-05-01 00:00:00
abstract：BACKGROUND:Over expression of Stathmin1 (STMN1), activation-induced cytidine deaminase (AID) and protein kinase C iota (PKCi) proteins participate in the regulation of carcinogenesis. In the present study, we investigated the expression of STMN1 in patients with gastric adenocarcinoma and also determined the correlatio...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
abstract：:Multidrug resistance (MDR) is a major clinical obstacle in the treatment of several cancers including hematological malignancies and solid tumors. The ATP-binding cassette transporter B1 (ABCB1) gene and its product, P-glycoprotein (P-gp), is one molecule that is involved in drug resistance. Here we report on the effe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:Angiogenesis has gained wide acceptance as a reliable prognostic factor in several solid tumors. However, to date, experience in pancreatic adenocarcinoma is limited. MATERIALS AND METHODS:Specimens from 45 patients radically operated on at our departments from 1988 to 1997 were stained immunohistochemicall...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:Copper transporters (CTR) also regulate the cellular transport of platinum drugs, but their role in platinum resistance of ovarian cancer has not been elucidated. MATERIALS AND METHODS:CTR expression in ovarian cancer tissues resected from patients treated by platinum-based chemotherapy was evaluated immuno...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：BACKGROUND/AIM:Detection of circulating tumor cells in the perioperative period predicts poor prognosis in patients with colorectal cancer. There is only one Food and Drug Administration-approved method for such detection, CellSearch, for which results have been inconsistent. A new immunological method, CytoTrack, has ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11666
更新日期：2017-06-01 00:00:00
abstract：:A consecutive series of 128 breast cancer patients detected by breast screening were enrolled in this study to analyze their age-dependent characteristics. They were classified into three groups; 40 years or less (young): 26 patients, 41-55 years (middle-aged): 67 patients and 56 years or over (old): 35 patients. The ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
