Abstract：
:Severe side effects of cocaine consumption are vasoocclusive events such as myocardial infarction and stroke. We have hypothesized that cocaine could affect red blood cells (RBCs) and alter the rheological behaviour of blood. Heparinized blood from healthy volunteers was incubated with a final hematocrit of 45% with increasing cocaine concentrations: 0, 10, 100, 1000, and 10'000 μmol/L plasma. Time dependence of the shape change was tested in phosphate buffered saline containing cocaine. RBCs were fixed in 1% glutaraldehyde for morphological analysis. Blood viscosity was measured with a Couette Viscometer (Contraves LS 30) at 37°C and a shear rate of 69.5 s⁻¹. RBC aggregation was assessed with a Myrenne aggregometer. Cocaine induced a dose-dependent stomatocytic shape transformation of RBCs, which was more pronounced in buffer than in plasma (plasma protein binding of the drug). Stomatocytosis occurs when a drug intercalates preferentially in the inner half of the membrane lipid bilayer. It was a time-dependent process with two components, an almost instant shape change occurring within 1 s, followed by a gradual further shape change during 10 min. Stomatocytosis was reversible by resuspension of the RBCs in cocaine-free buffer. This stomatocytic shape change increased whole blood viscosity at high shear rate from 5.69±0.31 mPa.s to 6.39±0.34 mPa.s for control and 10'000 μmol/L cocaine, respectively (p<0.01). RBC aggregation was not affected by the shape change. These effects occurred at a cocaine concentration, which is several-fold above those measured in vivo. Therefore, it is unlikely that hemorheological factors are involved in vascular events after cocaine consumption.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Cagienard F,Schulzki T,Furlong P,Reinhart WHdoi
10.3233/CH-2012-1638subject
Has Abstractpub_date
2013-01-01 00:00:00pages
321-9issue
3eissn
1386-0291issn
1875-8622pii
R4731M028348W230journal_volume
55pub_type
杂志文章abstract：BACKGROUND:Renal ischemia-reperfusion injury (IRI) is common in renal transplantation, shock, and nephrolithotomy. OBJECTIVE:To quantitatively assess rabbit renal IRI with contrast-enhanced ultrasound (CEUS) and investigate associations between renal cortical time-intensity curve (TIC) parameters and intracellular adh...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-131768
更新日期：2015-01-01 00:00:00
abstract：:Radiographic contrast media can lead to drastic changes of the morphology of erythrocytes. The change of the erythrocyte morphology is associated with a decreased deformability possibly resulting from distinctions in the loss of constituents of the membrane cytoskeleton. However, it is unclear whether there is an intr...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-141879
更新日期：2014-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
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doi：10.3233/CH-2010-1367
更新日期：2011-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-168118
更新日期：2016-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章,评审
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更新日期：2009-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章,评审
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journal_title：Clinical hemorheology and microcirculation
pub_type： 临床试验,杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-200857
更新日期：2020-07-09 00:00:00
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journal_title：Clinical hemorheology and microcirculation
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doi：10.3233/CH-2010-1326
更新日期：2010-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-151990
更新日期：2015-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2011-1438
更新日期：2012-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 临床试验,杂志文章
doi：
更新日期：2008-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-152018
更新日期：2016-10-05 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Vertigo of various and often unknown aetiologies has been associated with and attributed to impaired microvascular perfusion in the inner ear or the vertebrobasilar system. Vertigoheel is a low-dose combination preparation of proven value in the symptomatic treatment of vertigo. In the present study we tested the hypo...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2010-1330
更新日期：2010-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-151969
更新日期：2016-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
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更新日期：2015-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
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doi：10.3233/CH-189313
更新日期：2018-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章,评审
doi：10.3233/CH-2011-1512
更新日期：2012-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
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更新日期：2019-01-01 00:00:00
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doi：10.3233/CH-200875
更新日期：2020-09-08 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
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doi：10.3233/CH-180468
更新日期：2019-01-01 00:00:00
abstract：:Recently, it was shown that mesenchymal stem cells (MSCs) are capable of differentiating into endothelial cells which highlights the potential role of MSCs in neovascularization. In the present study, we investigated the paracrine factors responsible for tube formation in human adipose-tissue derived stem cells (ASCs)...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2011-1397
更新日期：2011-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
abstract：:The lecture was presented during the Fåhraeus award ceremony for 2016 at the University of Lisbon. It summarizes the main results and some of the more important hemorheological contributions achieved in the Laboratory of Biodynamics and Biorheology of the Institute of Mechanics to BAS and in collaboration with other l...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-168001
更新日期：2016-01-01 00:00:00
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journal_title：Clinical hemorheology and microcirculation
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更新日期：2012-01-01 00:00:00
