当前位置： SCI文献检索 > NEUROCHEMISTRY INTERNATIONAL期刊下所有文献 > Aralar mRNA and protein levels in neurons and astrocytes freshly isolated from young and adult mouse brain and in maturing cultured astrocytes.

Aralar mRNA and protein levels in neurons and astrocytes freshly isolated from young and adult mouse brain and in maturing cultured astrocytes.

Abstract：

:Intense glucose-based energy metabolism and glutamate synthesis by astrocytes require malate-aspartate-shuttle (MAS) activity to regenerate NAD⁺ from NADH formed during glycolysis, since brain lacks significant glycerophosphate shuttle activity. Aralar is a necessary aspartate/glutamate exchanger for MAS function in brain. Based on cytochemical immunoassays the absence of aralar in adult astrocytes was repeatedly reported. This would mean that adult astrocytes must regenerate NAD⁺ by producing lactate from pyruvate, eliminating its use by oxidative and biosynthetic pathways. We alternatively used astrocytes and neurons from adult brain, freshly isolated by fluorescence-activated cell sorting, to determine aralar protein by a specific antibody and its mRNA by real-time PCR. Both protein and mRNA expressions were identical in adult neurons and astrocytes and similar to whole brain levels. The same level of aralar expression was reached in well-differentiated astrocyte cultures, but not until late development, coinciding with the late-maturing brain capability for glutamate formation and degradation.

journal_name

Neurochem Int

journal_title

Neurochemistry international

authors

Li B,Hertz L,Peng L

doi

10.1016/j.neuint.2012.09.009

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1325-32

issue

8

eissn

0197-0186

issn

1872-9754

pii

S0197-0186(12)00294-X

journal_volume

61

pub_type

杂志文章

相关文献

NEUROCHEMISTRY INTERNATIONAL文献大全
  • GABA and the regulation of serotonin N-acetyltransferase activity in amphibian retina-I. Effects of GABA agonists and antagonists.

    abstract：:Retinal melatonin biosynthesis is regulated in part by changes in the activity of serotonin N-acetyltransferase (NAT), which increases at night in dark-adapted retinas, but not in light-exposed retinas. Using an in vitro preparation of Xenopus laevis (African clawed frog) eye cups, we have obtained evidence supporting...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(89)90174-5

    authors： Boatright JH,Iuvone PM

    更新日期：1989-01-01 00:00:00

  • Interactions between age, sex, and hormones in experimental ischemic stroke.

    abstract：:Age, sex, and gonadal hormones have profound effects on ischemic stroke outcomes, although how these factors impact basic stroke pathophysiology remains unclear. There is a plethora of inconsistent data reported throughout the literature, primarily due to differences in the species examined, the timing and methods use...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2012.10.003

    authors： Liu F,McCullough LD

    更新日期：2012-12-01 00:00:00

  • Cytosolic sialidase from the nerve endings of developing rat forebrain.

    abstract：:The developmental profile of cytosolic sialidase from the nerve ending and the nerve ending-free compartments of rat forebrain was studied from birth to 150 days. Soluble extracts containing the cytosol from nerve endings and nerve ending-free tissue were separately prepared; the recovery of cytosol in the two extract...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(83)90055-4

    authors： Venerando B,Fiorilli A,Malesci A,Goi GC,Lombardo A,Preti A,Tettamanti G

    更新日期：1983-01-01 00:00:00

  • Lercanidipine boosts the efficacy of mesenchymal stem cell therapy in 3-NP-induced Huntington's disease model rats via modulation of the calcium/calcineurin/NFATc4 and Wnt/β-catenin signalling pathways.

    abstract：:3-Nitropropionic acid (3-NP) induces a spectrum of Huntington's disease (HD)-like neuropathologies in the rat striatum. The present study aimed to demonstrate the neuroprotective effect of lercanidipine (LER) in rats with 3-NP-induced neurotoxicity, address the possible additional protective effect of combined treatme...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2019.104548

    authors： Elbaz EM,Helmy HS,El-Sahar AE,Saad MA,Sayed RH

    更新日期：2019-12-01 00:00:00

  • Effects of ifenprodil on the N-methyl-D-aspartate receptor ionophore complex in rat brain.

    abstract：:The effects of a cerebral anti-ischemic drug ifenprodil on the receptor ionophore complex of an N-methyl-D-aspartate (NMDA)-sensitive subclass of central excitatory amino acid receptors were examined using [3H](+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10- imine (MK-801) binding in rat brain synaptic memb...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(92)90076-4

    authors： Ogita K,Ohkawara A,Suzuki T,Ohgaki T,Uchida S,Meguri H,Yoneda Y

    更新日期：1992-07-01 00:00:00

  • Immunohistoblot analysis on whole human hemispheres from normal and Alzheimer diseased brains.

    abstract：:We demonstrate the feasibility and usefulness of the histoblot immunostaining of cryosections of whole hemispheres of healthy and Alzheimer diseased (AD) human brains by localizing a neuron-specific marker, the anti-neuronal nuclei (NeuN) antigen. As expected, cortical NeuN-immunopositive regions were generally thinne...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2008.09.002

    authors： Beliczai Z,Varszegi S,Gulyas B,Halldin C,Kasa P,Gulya K

    更新日期：2008-12-01 00:00:00

  • Gallamine and tubocurarine as possible allosteric modifiers of soluble acetylcholinesterase activity at physiological ionic strength.

    abstract：:Esters of dimethylcarbamic acid are known to be poor substrates of acetylcholinesterase. They carbamylate the active catalytic site of the enzyme and the subsequent decarbamylation is a slow but measurable process. Similarly, acetylcholinesterase can be phosphonylated, and the dephosphonylation is extremely slow. Rapi...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(81)90061-9

    authors： Dawson RM,Poretski M,Upsher CM

    更新日期：1981-01-01 00:00:00

  • Differences in kainate receptor involvement in hippocampal mossy fibre long-term potentiation depending on slice orientation.

    abstract：:Long-term potentiation (LTP) is a well-established experimental model used to investigate the synaptic basis of learning and memory. LTP at mossy fibre - CA3 synapses in the hippocampus is unusual because it is normally N-methyl-d-aspartate (NMDA) receptor-independent. Instead it seems that the trigger for mossy fibre...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2012.04.021

    authors： Sherwood JL,Amici M,Dargan SL,Culley GR,Fitzjohn SM,Jane DE,Collingridge GL,Lodge D,Bortolotto ZA

    更新日期：2012-09-01 00:00:00

  • Isolation of endogenous modulators for the GABAA and taurine receptors.

    abstract：:Several endogenous brain substances which inhibit [3H]muscimol binding were isolated, and one of them has been purified to apparent homogeneity. The purification involved the extraction of brain tissue with water, followed by several steps of gel filtration column chromatography and high performance liquid chromatogra...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(93)90134-q

    authors： Tang XW,Yarom M,Carlson RG,Vander Velde D,Huang PY,Lee YH,Seah EC,Deupree D,Wu JY

    更新日期：1993-11-01 00:00:00

  • Agonist binding fraction of dopamine D2/3 receptors in rat brain: a quantitative autoradiographic study.

    abstract：:There has arisen considerable interest in the study of dopamine D(2/3) agonist binding sites by positron emission tomography (PET), based on the claim that agonist sites represent a functional subset of the total number of sites labeled by more conventional antagonist ligands. To test the basis of this claim, we used ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2010.01.010

    authors： Minuzzi L,Cumming P

    更新日期：2010-05-01 00:00:00

  • Identification of a calcium binding protein highly localized to the cochlear nucleus.

    abstract：:A protein, designated as protein 10 (MW = 29 kDa, pI = 5.3), was shown to be highly localized to the cochlear nuclei of guinea-pigs and rats by the use of two-dimensional gel electrophoresis. In gels from guinea-pig brain, this protein appeared greatest in amounts in the ventral cochlear nucleus and within the deep re...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(89)90154-x

    authors： Winsky L,Nakata H,Jacobowitz DM

    更新日期：1989-01-01 00:00:00

  • Adeno-associated virus mediated gene transfer into primary rat brain neuronal and glial cultures: enhancement with the pH-sensitive surfactant dodecyl 2-(1'-imidazolyl) propionate.

    abstract：:This study evaluated the effects of a novel, pH-sensitive surfactant, dodecyl 2-(1'-imidazolyl) propionate (DIP), on cationic lipid mediated transfection in primary rat brain neuronal and glial cultures. The cationic lipid complex DOTAP/DOPE (1, 2-dioleoyl-3-trimethylammonium propionate and dioleoyl phosphatidylethano...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(00)00007-3

    authors： Wang S,Bui V,Hughes JA,King MA,Meyer EM

    更新日期：2000-07-01 00:00:00

  • Systemic pregabalin attenuates facial hypersensitivity and noxious stimulus-evoked release of glutamate in medullary dorsal horn in a rodent model of trigeminal neuropathic pain.

    abstract：:Pregabalin is effective in treating many neuropathic pain conditions. However, the mechanisms of its analgesic effects remain poorly understood. The aim of the present study was to determine whether pregabalin suppresses facial mechanical hypersensitivity and evoked glutamate release in the medullary dorsal horn (MDH)...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.02.022

    authors： Kumar N,Cherkas PS,Varathan V,Miyamoto M,Chiang CY,Dostrovsky JO,Sessle BJ,Coderre TJ

    更新日期：2013-05-01 00:00:00

  • The unresolved role of mitochondrial DNA in Parkinson's disease: An overview of published studies, their limitations, and future prospects.

    abstract：:Parkinson's disease (PD), a progressive neurodegenerative disorder, has long been associated with mitochondrial dysfunction in both sporadic and familial forms of the disease. Mitochondria are crucial for maintaining cellular homeostasis, and their dysfunction is detrimental to dopaminergic neurons. These neurons are ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2019.104495

    authors： Müller-Nedebock AC,Brennan RR,Venter M,Pienaar IS,van der Westhuizen FH,Elson JL,Ross OA,Bardien S

    更新日期：2019-10-01 00:00:00

  • Rat choline acetyltransferase of the peripheral type differs from that of the common type in intracellular translocation.

    abstract：:Choline acetyltransferase (ChAT), the synthesizing enzyme for acetylcholine, has been implicated to involve multiple isoforms of ChAT mRNA in several animals. Since these isoforms are mostly non-coding splice variants, only a homologous ChAT protein of about 68 kDa has been shown to be produced in vivo. Recent evidenc...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2004.11.006

    authors： Matsuo A,Bellier JP,Hisano T,Aimi Y,Yasuhara O,Tooyama I,Saito N,Kimura H

    更新日期：2005-04-01 00:00:00

  • AMN082 promotes the proliferation and differentiation of neural progenitor cells with influence on phosphorylation of MAPK signaling pathways.

    abstract：:Metabotropic glutamate receptors (mGluRs) are expressed in neural progenitor cells (NPCs) and may play important roles in the neurogenesis during embryonic development and adult brain repair following injuries. In the present study, we investigated the expression of metabotropic glutamate receptor 7 (mGluR7) and the p...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2010.04.004

    authors： Tian Y,Liu Y,Chen X,Kang Q,Zhang J,Shi Q,Zhang H

    更新日期：2010-08-01 00:00:00

  • In vitro neurotransmitter release in an animal model of depression.

    abstract：:Sprague-Dawley rats exposed to uncontrollable shock can be separated by a subsequent shock escape test into two groups: a "helpless" (LH) group which demonstrates a deficit in escape behavior, and a "nonlearned helpless" (NLH) group which shows no escape deficit and acquires the escape response as readily as naive con...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(92)90065-y

    authors： Edwards E,Kornrich W,van Houtten P,Henn FA

    更新日期：1992-07-01 00:00:00

  • Posttranslational processing of proenkephalins and chromogranins/secretogranins.

    abstract：:Posttranslational processing of peptide-precursors is nowadays believed to play an important role in the functioning of neurons and endocrine cells. Both proenkephalins and chromogranins/secretogranins are considered as precursor molecules in these tissues, resulting in posttranslationally formed degradation products ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/0197-0186(93)90016-x

    authors： Dillen L,Miserez B,Claeys M,Aunis D,De Potter W

    更新日期：1993-04-01 00:00:00

  • Glycoprotein synthesis and postsynaptic remodelling in long-term memory.

    abstract：:This paper describes the background in experiment and theory on which present-day studies of the biochemistry of learning and memory are based. It then reviews specifically the use of passive avoidance training in the day-old chick as a model for the analysis of the cellular events associated with memory-formation. It...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(89)90055-7

    authors： Rose SP

    更新日期：1989-01-01 00:00:00

  • The in vivo modulation of dopamine synthesis by calcium ions: influences on the calcium independent release.

    abstract：:To investigate the contribution of the dopamine (DA) synthesis to both the calcium-dependent and the carrier-mediated, mechanisms of DA release in the striatum, anaesthetized rats were locally superfused in the striatum with a push pull cannula supplied with an artificial CSF containing tritiated tyrosine. DA, dihydro...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(99)00094-7

    authors： Olivier V,Gobert A,Guibert B,Leviel V

    更新日期：1999-12-01 00:00:00

  • The regulation of corticosteroid receptors in response to chronic social defeat.

    abstract：:Our previous studies demonstrated that chronic social defeat (CSD) up-regulated expression of the serotonin transporter (SERT) and norepinephrine transporter (NET) in the brain, which was mediated by corticosteroid receptors. In the present study we first analyzed the alterations of corticosteroid receptors in differe...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2017.05.021

    authors： Zhang J,Fan Y,Raza MU,Zhan Y,Du XD,Patel PD,Zhu MY

    更新日期：2017-09-01 00:00:00

  • Are nestin-positive mesenchymal stromal cells a better source of cells for CNS repair?

    abstract：:In recent years there has been a great deal of research within the stem cell field which has led to the definition and classification of a range of stem cells from a plethora of tissues and organs. Stem cells, by classification, are considered to be pluri- or multipotent and have both self-renewal and multi-differenti...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2016.08.001

    authors： Lindsay SL,Barnett SC

    更新日期：2017-06-01 00:00:00

  • The venom of Conus pennaceus inhibits the binding of [3H]neuropeptide Y by direct interaction with the radioligand.

    abstract：:The venom from the marine snail Conus pennaceus inhibits the binding of [3H]neuropeptide Y to calf brain membranes (Czerwiec et al., 1996a) and, in the present study, also to rat forebrain membranes. These membranes contain about 80% Y1- and 20% Y2-receptors. The inhibition by the venom was concentration-dependent wit...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(97)00063-6

    authors： Diallo B,Vanderheyden PM,De Backer JP,Vauquelin G

    更新日期：1998-01-01 00:00:00

  • P400 protein is one of the major substrates for Ca2+/calmodulin-dependent protein kinase II in the postsynaptic density-enriched fraction isolated from rat cerebral cortex, hippocampus and cerebellum.

    abstract：:Concanavalin A-binding glycoprotein with 250 K M(r) found in the postsynaptic density (PSD)-enriched preparation (or synaptic cytoskeleton) from rat cerebellum was identified with P400 protein from the physicochemical properties and enrichment in the cerebellum. Proteins homologous to the cerebellar 250 K M(r) protein...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(92)90126-c

    authors： Suzuki T,Abe-Dohmae S,Tanaka R

    更新日期：1992-01-01 00:00:00

  • Systemic L-buthionine-S-R-sulfoximine administration modulates glutathione homeostasis via NGF/TrkA and mTOR signaling in the cerebellum.

    abstract：:Glutathione (GSH) is an essential component of intracellular antioxidant systems that plays a primordial role in the protection of cells against oxidative stress, maintaining redox homeostasis and xenobiotic detoxification. GSH synthesis in the brain is limited by the availability of cysteine and glutamate. Cystine, t...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2018.10.007

    authors： Garza-Lombó C,Petrosyan P,Tapia-Rodríguez M,Valdovinos-Flores C,Gonsebatt ME

    更新日期：2018-12-01 00:00:00

  • Release of acid-soluble radioactivity from rapidly transported proteins in homogenates of rabbit superior colliculus.

    abstract：:An intraocular injection of [(3)H]proline was used to label rabbit retinal ganglion cells including components subjected to axonal transport. Degradation of rapidly axonally transported labelled proteins was estimated in an in vitro system using homogenates of the nerve terminal regions. The energy requirements of thi...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(85)90061-0

    authors： Gustavsson S,Karlsson JO

    更新日期：1985-01-01 00:00:00

  • Direct demonstration by [13C]NMR spectroscopy that glutamine from astrocytes is a precursor for GABA synthesis in neurons.

    abstract：:Primary cultures of cerebral cortical astrocytes and neurons, as well as neurons growing on top of the astrocytes (sandwich co-cultures), were incubated with 1-[13C]glucose or 2-[13C]acetate and in the presence or absence of the glutamine synthetase inhibitor methionine sulfoximine. [13C]NMR spectroscopy at 125 MHz wa...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(93)90064-c

    authors： Sonnewald U,Westergaard N,Schousboe A,Svendsen JS,Unsgård G,Petersen SB

    更新日期：1993-01-01 00:00:00

  • BN52021, a platelet activating factor antagonist, is a selective blocker of glycine-gated chloride channel.

    abstract：:We have found that the platelet activating factor antagonist (BN52021) is an effective blocker of the glycine (Gly) receptor-mediated responses in the hippocampal pyramidal neurons of rat. Using the whole-cell voltage clamp and concentration clamp recording techniques, we investigated the mechanism underlying the inhi...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(01)00109-7

    authors： Kondratskaya EL,Lishko PV,Chatterjee SS,Krishtal OA

    更新日期：2002-06-01 00:00:00

  • Neurotensin concentrations in rat striatum and nucleus accumbens: Further studies of their regulation.

    abstract：:The possible influence of cholinergic and dopaminergic mechanisms on neurotensin-containing neurones was examined at two different levels; nucleus accumbens and striatum in the rat brain. The acute treatment with the anticholinergic drugs atropine and scopolamine increased neurotensin concentrations in the striatum an...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(88)90145-3

    authors： Frey P,Lis M,Coward DM

    更新日期：1988-01-01 00:00:00

  • Therapeutic hypothermia protects photoreceptors through activating Cirbp pathway.

    abstract：:Therapeutic hypothermia as a physical method to lower the brain temperature of patients has been widely used in clinics as an effective and necessary step during the treatment of acute brain injury or edema. However, due to limitations of the ocular structure, the application of hypothermia in retinal neuroprotection ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2019.03.006

    authors： Sun YJ,Ma S,Fan B,Wang Y,Wang SR,Li GY

    更新日期：2019-06-01 00:00:00