Abstract:
BACKGROUND:Compliance with cleaning of flexible endoscope channels cannot be verified using visual inspection. Adenosine triphosphate (ATP) has been suggested as a possible rapid cleaning monitor for flexible endoscope channels. There have not been published validation studies to specify the level of ATP that indicates inadequate cleaning has been achieved. OBJECTIVE:The objective of this study was to validate the Clean-Trace (3M Inc, St. Paul, MN) ATP water test method for monitoring manual cleaning of flexible endoscopes. METHODS:This was a simulated use study using a duodenoscope as the test device. Artificial test soil containing 10(6) colony-forming units of Pseudomonas aeruginosa and Enterococcus faecalis was used to perfuse all channels. The flush sample method for the suction-biopsy (L1) or air-water channel (L2) using 40 and 20 mLs sterile reverse osmosis water, respectively, was validated. Residuals of ATP, protein, hemoglobin, and bioburden were quantitated from channel samples taken from uncleaned, partially cleaned, and fully cleaned duodenoscopes. The benchmarks for clean were as follows: <6.4 μg/cm(2) protein, <2.2 μg/cm(2) hemoglobin, and <4-log10 colony-forming units/cm(2) bioburden. RESULTS:The average ATP in clean channel samples was 27.7 RLUs and 154 RLUs for L1 and L2, respectively (<200 RLUs for all channels). The average protein, hemoglobin, and bioburden benchmarks were achieved if <200 RLUs were detected. If the channel sample was >200 RLUs, the residual organic and bioburden levels would exceed the acceptable benchmarks. CONCLUSION:Our data validated that flexible endoscopes that have complete manual cleaning will have <200 RLUs by the Clean-Trace ATP test.
journal_name
Am J Infect Controljournal_title
American journal of infection controlauthors
Alfa MJ,Fatima I,Olson Ndoi
10.1016/j.ajic.2012.03.018subject
Has Abstractpub_date
2013-03-01 00:00:00pages
245-8issue
3eissn
0196-6553issn
1527-3296pii
S0196-6553(12)00774-2journal_volume
41pub_type
杂志文章abstract::Nurses satisfied with their jobs report less job stress, more effective nurse-physician collaboration, and higher patient satisfaction scores. It is unknown if job satisfaction influences adherence to best practices or patient outcomes. This secondary data analysis investigated the relationship between job satisfactio...
journal_title:American journal of infection control
pub_type: 杂志文章
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journal_title:American journal of infection control
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journal_title:American journal of infection control
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journal_title:American journal of infection control
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journal_title:American journal of infection control
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journal_title:American journal of infection control
pub_type: 杂志文章
doi:10.1016/j.ajic.2006.02.004
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journal_title:American journal of infection control
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journal_title:American journal of infection control
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journal_title:American journal of infection control
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pub_type: 临床试验,杂志文章
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journal_title:American journal of infection control
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journal_title:American journal of infection control
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journal_title:American journal of infection control
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