Abstract：
:Effective and safe novel adjuvants are of great interest to the vaccine research community. In this study, we describe our evaluation of adjuvant formulations containing a TLR9 agonist adjuvant (ISS1018) or ISCOMATRIX™ adjuvant for a two-dose regimen of hepatitis B virus surface antigen virus-like particle vaccine in mice and rhesus macaques. Our results show a 10-20 fold improvement in Ab binding titers determined in an antigen-sandwich assay for adjuvant formulations with ISCOMATRIX™ adjuvant, in comparison to routine aluminum formulation. Furthermore, we optimized a competition assay to evaluate a functional component of immune sera, using a conformation-dependent and protective mAb, RFHBs1, as the probe. Although good correlation was observed between Ab binding titers from the antigen-sandwich assay and functional titers from the in-solution competition against RFHBs1, the latter assessment provided a much more stringent ranking of adjuvant formulations than the former. These results indicate the importance of evaluating functional Abs when assessing and comparing novel adjuvant formulations, as it provides another angle to investigate the effects of change in adjuvant composition on antigenic integrity of the testing vaccines.
journal_name
Vaccinejournal_title
Vaccineauthors
Freed DC,Towne VM,Casimiro DR,Zhao Q,Fu TMdoi
10.1016/j.vaccine.2011.09.122subject
Has Abstractpub_date
2011-11-21 00:00:00pages
9385-90issue
50eissn
0264-410Xissn
1873-2518pii
S0264-410X(11)01577-5journal_volume
29pub_type
杂志文章相关文献
VACCINE文献大全abstract：:The aim of this study was to evaluate an experimental Salmonella Enteritidis (SE) bacterin and an indirect ELISA system to assess quantitatively the acquired immunity in Awassi ewes to the vaccine and/or Echinacea purpurea (EP) dried roots. Four treatments of the ewes were included in the experimental design, with 6 e...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.03.035
更新日期：2015-05-05 00:00:00
abstract：:Several publications have reported recently the apparently successful use of human diploid cell rabies vaccine (HDCV) in volumes of less than the 6 X 1.0 cm3 dosage schedule currently recommended by the World Health Organization for post-exposure rabies prophylaxis. The newer proposed treatments are advocated to econo...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(85)90035-0
更新日期：1985-09-01 00:00:00
abstract：:Genetically modified bacterial flagellin (Fla), a Toll-like receptor-5 (TLR5) ligand, was evaluated as a fusion partner for human papillomavirus (HPV) L2-based immunogens in two animal challenge models; either cutaneous inoculation of rabbits with HPV 'quasivirions' containing cottontail rabbit papillomavirus (CRPV) g...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.04.032
更新日期：2014-06-12 00:00:00
abstract：:Pertactin/P.69, a surface-associated polypeptide antigen of Bordetella pertussis, was expressed in a Salmonella typhimurium aroA aroD vaccine strain, BRD509, using different expression systems, and the immune response in mice against these constructs was compared. Initially, Pertactin/P.69 was expressed on the surface...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(96)00036-9
更新日期：1996-10-01 00:00:00
abstract：:Assuming that the level of Bacille Calmette Guerin (BCG) coverage gives a measure of access to immunisation services, it is possible to derive what fraction of infants are not immunised against measles due to under-utilisation of existing services (rather than unavailability of services). According to the most recent ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00264-x
更新日期：2001-10-12 00:00:00
abstract：:To evaluate the perceived pain, other adverse events, and immunogenicity of influenza virus vaccine administered by needle-free jet injector (JI) compared with that of vaccine administered by needle and syringe (N&S), we randomly assigned 304 healthy young adults to receive one of three dosages (0.5, 0.3, or 0.2 ml) o...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0264-410x(01)00225-0
更新日期：2001-09-14 00:00:00
abstract：:There has been controversy about whether cognitive changes occur in early human immunodeficiency virus (HIV) disease. In those studies reporting cognitive changes, these are typically subclinical, and their relationship to daily and/or occupational functioning has not been addressed. The potential effects of changes m...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(93)90231-l
更新日期：1993-01-01 00:00:00
abstract：:The vast majority of vaccines used throughout the world are supplied by the private sector. It is essential therefore that the industry is closely engaged in future policy developments at a national and international level and is able to respond to the changing needs and priorities that may be required to ensure the s...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2016.10.084
更新日期：2017-01-20 00:00:00
abstract：BACKGROUND:One of the most concerning public health issues, related to vaccination and disease prevention, is the inability to induce durable immune responses following a single-dose immunization. In this regard, the nature of the inflammatory environment induced by vaccine adjuvants can negatively impact the resulting...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2019.04.004
更新日期：2019-05-06 00:00:00
abstract：:Rhesus-human rotavirus reassortants incorporating the gene expressing the VP7 surface protein of human rotavirus serotypes 1 or 2, and the remaining ten genes from rhesus rotavirus (RRV) were evaluated as candidate oral vaccines in 2-4-month-old infants. A single dose of the serotype 1 reassortant vaccine which had a ...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/0264-410x(91)90060-j
更新日期：1991-05-01 00:00:00
abstract：INTRODUCTION:Diarrhea is one of the leading causes of death in children under 5, and an estimated 39% of these deaths are attributable to rotavirus. Currently two live, oral rotavirus vaccines have been introduced on the market; however, the herd immunity effect associated with rotavirus vaccine has not yet been quanti...
journal_title：Vaccine
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.vaccine.2015.06.064
更新日期：2015-07-31 00:00:00
abstract：:The recombinant vaccinia viruses expressing the surface protein of Neospora caninum tachyzoite, NcSAG1 or NcSRS2, were constructed. The vaccination with these recombinant viruses could protect effectively the parasite invasion in a mouse model system. The vaccine efficacy of NcSRS2 was higher than that of NcSAG1. The ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00389-3
更新日期：2001-01-08 00:00:00
abstract：:A soluble fraction obtained from Bordetella pertussis was evaluated as adjuvant for the pertussis component of the Diphtheria-Pertussis-Tetanus (DPT) vaccine. High levels of antibodies were induced, and a 78% protection rate of mice challenged with live B. pertussis was observed. Two proteins were identified as the 73...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2006.08.033
更新日期：2007-01-08 00:00:00
abstract：:Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute encephalitis with high mortality in humans. To understand the virus-host interactions that influence JEV virulence, we determined the lethality of a neurovirulent (RP-9) and an attenuated (RP-2ms) variant of JEV in several immunodeficie...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.03.007
更新日期：2009-05-11 00:00:00
abstract：:Pertussis vaccine development has mainly focused on Bordetella pertussis, and consequently these vaccines contain B. pertussis antigens only. However, the related species Bordetella parapertussis can also cause pertussis, although symptoms associated with the disease are generally considered to be milder. Recent field...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2003.11.005
更新日期：2004-05-07 00:00:00
abstract：:Since 2004, general varicella vaccination has been recommended for all children 11-14 months of age in Germany. The objective of this study was to examine vaccination coverage in children and factors associated with parental acceptance during the first years after recommendation. In a regional surveillance area, cross...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.06.007
更新日期：2010-08-09 00:00:00
abstract：:Salmonella Enteritidis mutants with deletions in phoP, fliC or phoPfliC were tested for their virulence and their ability to induce parameters of the innate and adaptive immunity in addition to their potential for serological differentiation between vaccinated, non-vaccinated and infected chickens. The double phoPfliC...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.02.030
更新日期：2011-04-12 00:00:00
abstract：:Treatment of cervical cancer greatly varies according to the stage of the disease. Laparoscopic surgical staging is emerging as a valid approach, compared to clinical and imaging staging, to better identify the treatment plan. Minimally invasive surgery plays the greatest role in the treatment of early cervical carcin...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2008.11.110
更新日期：2009-05-29 00:00:00
abstract：:In order to evaluate the long-term immunity 2 and 3 years after booster vaccination against tick-borne encephalitis (TBE) following time intervals 3 years and longer since last TBE immunization, 195 (mean age 50.5+/-15.1 years) and 240 subjects (mean age 47.0+/-15.4 years), respectively, who had received a single boos...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.01.116
更新日期：2007-06-28 00:00:00
abstract：BACKGROUND:Alternative methods for influenza vaccine production are needed to ensure adequate supplies. METHODS:Healthy adults 50-64 years were assigned randomly to receive one intramuscular injection of trivalent recombinant hemagglutinin (rHA) or U.S. licensed trivalent inactivated vaccine (TIV) containing H1, H3 an...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.vaccine.2011.01.039
更新日期：2011-03-09 00:00:00
abstract：:Clinical and serum antibody responses following intramuscular injection of two formulations of Salmonella typhi Vi capsular polysaccharide (Vi) were assessed in a double-blind evaluation. Healthy adults were randomly assigned to receive a 25 micrograms dose of liquid (Vi-Liq; n = 182) or freeze-dried Vi vaccine (Vi-Ly...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0264-410x(94)90194-5
更新日期：1994-01-01 00:00:00
abstract：:Two cyclic peptide analogues corresponding to residues 139-146 (site A) of influenza A virus haemagglutinin (strain X31) were synthesized. The ability of these peptides to react with anti-influenza virus antibodies was found to depend on the conformation of the loop and on the orientation in which the peptide was pres...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(90)90086-2
更新日期：1990-08-01 00:00:00
abstract：:Improving the ability of DNA-based vaccines to induce potent Type1/Th1 responses against intracellular pathogens in large outbred species is essential. Rhodoccocus equi and equine infectious anemia virus (EIAV) are two naturally occurring equine pathogens that also serve as important large animal models of neonatal im...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.07.055
更新日期：2007-10-23 00:00:00
abstract：:The FML antigen of Leishmania donovani, in combination with either Riedel de Haën (R), QuilA, QS21 saponins, IL12 or BCG, was used in vaccination of an outbred murine model against visceral leishmaniasis (VL). Significant and specific increases in anti-FML IgG and IgM responses were detected for all adjuvants, and in ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(02)00444-9
更新日期：2002-11-22 00:00:00
abstract：:Biodegradable nanoparticles coated with proteins represent a promising method for in vivo delivery of vaccines. Here we used a rabbit model to compare quantitatively and qualitatively the antibody responses induced by poly(D,L-lactide) nanoparticles (PLA) and by emulsion adjuvant MF59 using three HIV-1 antigens: p24ga...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.08.060
更新日期：2007-10-23 00:00:00
abstract：:Effective antigen delivery is one of the most important issues in vaccine development. It has been suggested that adjuvant action results from a depot effect by prolonging the duration of the interaction between antigen and cells, and thus is related to the antigen-releasing properties of emulsion adjuvants. The objec...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.09.007
更新日期：2005-04-08 00:00:00
abstract：:Serological results obtained in a single laboratory from twin-studies on maternal immunisation, in Vietnam and Belgium offer the opportunity to compare antibody kinetics in infants before and after infant vaccination in the presence of vaccine-induced maternal antibodies. Nonlinear mixed-effects models (NLMMs) making ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.09.003
更新日期：2020-10-21 00:00:00
abstract：:A total of 105 hybridomas secreting anti-Japanese encephalitis (JE) virus monoclonal antibodies (mAbs) were generated from six fusions against four strains of JE virus: wild-type strains SA14 and G8924 and live attenuated vaccines SA14-5-3 and SA14-14-2 (PDK-9). Most of the mAbs (87%) elicited haemagglutination inhibi...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(92)90372-q
更新日期：1992-01-01 00:00:00
abstract：INTRODUCTION:Adolescents and young adults are at a high risk of developing human papillomavirus (HPV) infections, which can be prevented with the use of vaccines. Moreover, a combined immunization strategy for administration of HPV vaccines with other routine vaccines may lead to better compliance. We aim to comprehens...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2019.10.092
更新日期：2020-01-10 00:00:00
abstract：:The carbohydrate moieties on HIV-1 envelope glycoprotein (Env) act as shields to mask conserved neutralizing epitopes, while the hyperimmunogenic variable regions are immunodominant in inducing non-neutralizing antibodies, representing the major challenge for using Env as a vaccine candidate to induce broadly neutrali...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2019.09.073
更新日期：2019-12-03 00:00:00
