Evaluating functional antibodies in rhesus monkeys immunized with hepatitis B virus surface antigen vaccine with novel adjuvant formulations.


:Effective and safe novel adjuvants are of great interest to the vaccine research community. In this study, we describe our evaluation of adjuvant formulations containing a TLR9 agonist adjuvant (ISS1018) or ISCOMATRIX™ adjuvant for a two-dose regimen of hepatitis B virus surface antigen virus-like particle vaccine in mice and rhesus macaques. Our results show a 10-20 fold improvement in Ab binding titers determined in an antigen-sandwich assay for adjuvant formulations with ISCOMATRIX™ adjuvant, in comparison to routine aluminum formulation. Furthermore, we optimized a competition assay to evaluate a functional component of immune sera, using a conformation-dependent and protective mAb, RFHBs1, as the probe. Although good correlation was observed between Ab binding titers from the antigen-sandwich assay and functional titers from the in-solution competition against RFHBs1, the latter assessment provided a much more stringent ranking of adjuvant formulations than the former. These results indicate the importance of evaluating functional Abs when assessing and comparing novel adjuvant formulations, as it provides another angle to investigate the effects of change in adjuvant composition on antigenic integrity of the testing vaccines.






Freed DC,Towne VM,Casimiro DR,Zhao Q,Fu TM




Has Abstract


2011-11-21 00:00:00
















  • Development of an in process control filtration-assisted chemiluminometric immunoassay to quantify foot and mouth disease virus (FMDV) non-capsid proteins in vaccine-antigen batches.

    abstract::In many countries, foot and mouth disease (FMD) is controlled by vaccination and surveillance against non-capsid proteins (NCP); therefore vaccines are required not to induce antibodies against NCP. Vaccine purity is evaluated by repeated inoculation of naïve cattle, an expensive and time consuming protocol that raise...


    pub_type: 杂志文章


    authors: Capozzo AV,Martínez MR,Schielen WJ

    更新日期:2010-09-14 00:00:00

  • Co-delivery of plasmid-encoded cytokines modulates the immune response to a DNA vaccine delivered by in vivo electroporation.

    abstract::In this study, in vivo electroporation of a DNA vaccine adjuvanted with plasmids encoding different cytokines was investigated in large animals. Sheep were injected intramuscularly with a DNA vaccine encoding an antigen of Haemonchus contortus (pNPA) and plasmids encoding different cytokines followed by in vivo electr...


    pub_type: 杂志文章


    authors: Yen HH,Scheerlinck JP

    更新日期:2007-03-30 00:00:00

  • Duration of protection of rabbits after vaccination with Bacillus anthracis recombinant protective antigen vaccine.

    abstract::Long-term protection of rabbits that had been vaccinated with two doses of a recombinant protective antigen (rPA) vaccine was examined against an aerosol spore challenge with the Ames isolate of Bacillus anthracis at 6 and 12 months. At 6 months after the primary injection, survival was 74.1% (20/27) with quantitative...


    pub_type: 杂志文章


    authors: Little SF,Ivins BE,Webster WM,Fellows PF,Pitt ML,Norris SL,Andrews GP

    更新日期:2006-03-24 00:00:00

  • A combined DNA vaccine enhances protective immunity against Mycobacterium tuberculosis and Brucella abortus in the presence of an IL-12 expression vector.

    abstract::We examined the immunogenicity and protective efficacy of a combined DNA vaccine that included six genes encoding immunodominant antigens from Mycobacterium tuberculosis and Brucella abortus. The IL-12 adjuvant system was used for immunization in combination with the combined DNA vaccine (DNA-IL-12(+)). Mice immunized...


    pub_type: 杂志文章


    authors: Yu DH,Li M,Hu XD,Cai H

    更新日期:2007-09-17 00:00:00

  • Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model.

    abstract::Schistosomiasis is an important parasitic disease for which there is no available vaccine. We have focused on a functionally important antigen of Schistosoma mansoni, Sm-p80, as a vaccine candidate because of its consistent immunogenicity, protective potential and antifecundity effect observed in murine models; and fo...


    pub_type: 杂志文章


    authors: Ahmad G,Zhang W,Torben W,Damian RT,Wolf RF,White GL,Chavez-Suarez M,Kennedy RC,Siddiqui AA

    更新日期:2009-05-11 00:00:00

  • Arguments and sources on Italian online forums on childhood vaccinations: Results of a content analysis.

    abstract:BACKGROUND:Despite being committed to the immunization agenda set by the WHO, Italy is currently experiencing decreasing vaccination rates and increasing incidence of vaccine-preventable diseases. Our aim is to analyze Italian online debates on pediatric immunizations through a content analytic approach in order to qua...


    pub_type: 杂志文章


    authors: Fadda M,Allam A,Schulz PJ

    更新日期:2015-12-16 00:00:00

  • Confounder adjustment in vaccine safety studies: comparing three offset terms for case-centered approach.

    abstract::The case-centered approach (CCA) can be useful in vaccine safety studies to adjust for time varying confounders, especially seasonality. The method compares the observed odds of vaccination in the time interval prior to the event with the expected odds of vaccination during this interval. The log of the expected odds ...


    pub_type: 杂志文章


    authors: Qian L,Tseng HF,Sy LS,Jacobsen SJ

    更新日期:2013-01-02 00:00:00

  • Control of tick infestations in wild roe deer (Capreolus capreolus) vaccinated with the Q38 Subolesin/Akirin chimera.

    abstract::Ticks (Acari: Ixodidae) are considered to be the most important vectors of disease-causing pathogens in domestic and wild animals, and emerging and re-emerging tick-borne diseases (TBD) exert an enormous impact on them. Wild ungulates are hosts for a wide variety of tick species and tick-borne pathogens that affect hu...


    pub_type: 杂志文章


    authors: Contreras M,San José C,Estrada-Peña A,Talavera V,Rayas E,Isabel León C,Luis Núñez J,García Fernández de Mera I,de la Fuente J

    更新日期:2020-09-22 00:00:00

  • Modelling the effect of discontinuing universal Bacillus Calmette-Guérin vaccination in an intermediate tuberculosis burden setting.

    abstract:BACKGROUND:Bacillus Calmette-Guérin (BCG) vaccination is a widely-used public health intervention for tuberculosis (TB) control. In Taiwan, like other intermediate TB burden settings, steadily declining TB incidence raises important questions on whether universal BCG vaccination should be discontinued. Recent surveys o...


    pub_type: 杂志文章


    authors: Fu H,Lin HH,Hallett TB,Arinaminpathy N

    更新日期:2018-09-18 00:00:00

  • Structural perturbation of diphtheria toxoid upon adsorption to aluminium hydroxide adjuvant.

    abstract::Aluminium-containing adjuvants are often used to enhance the potency of vaccines. In the present work we studied whether adsorption of diphtheria toxoid to colloidal aluminium hydroxide induces conformational changes of the antigen. Diphtheria toxoid has a high affinity for the aluminium hydroxide particles based on a...


    pub_type: 杂志文章


    authors: Régnier M,Metz B,Tilstra W,Hendriksen C,Jiskoot W,Norde W,Kersten G

    更新日期:2012-11-06 00:00:00

  • Universal measles-mumps-rubella vaccination to new recruits and the incidence of mumps in the military.

    abstract::In response to the resurgence of mumps, the Korean Armed Forces started the measles-mumps-rubella (MMR) vaccination to all new recruits regardless of prior vaccination history. We evaluated the effectiveness of the vaccination by comparing the incidence between the military and civilian populations before and after im...


    pub_type: 杂志文章


    authors: Moon JY,Jung J,Huh K

    更新日期:2017-07-13 00:00:00

  • Preparation-free method can enable rapid surfactant screening during industrial processing of influenza vaccines.

    abstract::Triton X-100 (TX-100) is the most common surfactant used to split viruses during the production of influenza split-virus vaccines. It is a mild surfactant not known to denature the viral proteins; this property makes TX-100 useful for maintaining antigen conformational structure, and, as an added benefit, for partiall...


    pub_type: 杂志文章


    authors: Sahin Z,Neeleman R,Haines J,Kayser V

    更新日期:2019-02-14 00:00:00

  • Immunogenicity and safety of the quadrivalent meningococcal vaccine MenACWY-TT co-administered with a combined diphtheria-tetanus-acellular pertussis vaccine versus their separate administration in adolescents and young adults: A phase III, randomized stu

    abstract:BACKGROUND:This study evaluated the immunogenicity and safety of quadrivalent meningococcal conjugate vaccine using tetanus (T) toxoid as carrier protein (MenACWY-TT) co-administered with combined diphtheria-tetanus-acellular pertussis vaccine (Tdap) versus their separate administration in adolescents and young adults....


    pub_type: 杂志文章,随机对照试验


    authors: Rivera L,Schwarz TF,Kim KH,Kim YK,Behre U,Cha SH,Jo DS,Lee J,Lee JS,Cheuvart B,Jastorff A,Van der Wielen M

    更新日期:2018-07-25 00:00:00

  • Reactions to small pox vaccine in naïve and previously-vaccinated individuals.

    abstract::Two hundred health care workers in England and Wales were vaccinated with the Lister/Elstree strain of the vaccinia virus, and completed health diaries for 21 days or until the lesion had scabbed over. Pain and temperature were measured daily, and all other symptoms recorded freehand by the vaccinee. One hundred and f...


    pub_type: 杂志文章


    authors: Auckland C,Cowlishaw A,Morgan D,Miller E

    更新日期:2005-07-14 00:00:00

  • Towards the rational design of Th1 adjuvants.

    abstract::Finding adjuvants in order to enhance immune responses against target immunogens has been a major and recurrent issue for the vaccine industry. It is yet to be solved, most particularly in the context of a growing interest in designing new types of vaccines capable of eliciting Th1 immune responses. A review of synthe...


    pub_type: 杂志文章,评审


    authors: Moingeon P,Haensler J,Lindberg A

    更新日期:2001-08-14 00:00:00

  • Use of a current varicella vaccine as a live polyvalent vaccine vector.

    abstract::Varicella-zoster virus (VZV) is the causative agent of varicella and zoster. The varicella vaccine was developed to control VZV infection in children. The currently available Oka vaccine strain is the only live varicella vaccine approved by the World Health Organization. We previously cloned the complete genome of the...


    pub_type: 杂志文章,评审


    authors: Murakami K,Mori Y

    更新日期:2016-01-04 00:00:00

  • Clinical types of HBsAg-positive hepatitis.

    abstract::Hepatitis B is a parenterally and sexually transmitted disease of global importance. It associates with hepatitis D in a consistent proportion of cases. The disease most frequently runs a subclinical and anicteric course, with a significant number of cases that become chronic. Chronic hepatitis may progress to cirrhos...


    pub_type: 杂志文章,评审


    authors: Nisini R,Rizzetto M

    更新日期:1993-01-01 00:00:00

  • Evaluation of adenovirus 19a as a novel vector for mucosal vaccination against influenza A viruses.

    abstract::Since preexisting immunity and enhanced infection rates in a clinical trial of an HIV vaccine have raised some concerns on adenovirus (Ad) serotype 5-based vaccines, we evaluated the subgroup D adenovirus serotype Ad19a for its suitability as novel viral vector vaccine against mucosal infections. In BALB/c mice, we co...


    pub_type: 杂志文章


    authors: Lapuente D,Ruzsics Z,Thirion C,Tenbusch M

    更新日期:2018-05-03 00:00:00

  • Recombinant vesicular stomatitis virus-based west Nile vaccine elicits strong humoral and cellular immune responses and protects mice against lethal challenge with the virulent west Nile virus strain LSU-AR01.

    abstract::Vesicular stomatitis virus (VSV) has been extensively utilized as a viral vector system for the induction of protective immune responses against a variety of pathogens. We constructed recombinant VSVs specifying either the Indiana or Chandipura virus G glycoprotein and expressing the West Nile virus (WNV) envelope (E)...


    pub_type: 杂志文章


    authors: Iyer AV,Pahar B,Boudreaux MJ,Wakamatsu N,Roy AF,Chouljenko VN,Baghian A,Apetrei C,Marx PA,Kousoulas KG

    更新日期:2009-02-05 00:00:00

  • Achieving a "Grand Convergence" in global health by 2035.

    abstract::Historical evidence demonstrates the validity of the concept "Grand Convergence". The Lancet commission identified the major challenges facing low and lower middle income countries including the unfinished agenda, non-communicable diseases and injuries and an approach to funding and achieving these objectives along wi...


    pub_type: 杂志文章


    authors: Mahmoud A

    更新日期:2017-01-20 00:00:00

  • Challenges and opportunities in RSV vaccine development: Meeting report from FDA/NIH workshop.

    abstract::Respiratory syncytial virus (RSV) is the most common cause of serious acute lower respiratory illness in infants and young children and a significant cause of disease burden in the elderly and immunocompromised. There are no licensed RSV vaccines to address this significant public health need. While advances in vaccin...


    pub_type: 杂志文章


    authors: Roberts JN,Graham BS,Karron RA,Munoz FM,Falsey AR,Anderson LJ,Marshall V,Kim S,Beeler JA

    更新日期:2016-09-22 00:00:00

  • Vaccine adverse events in a safety net healthcare system and a managed care organization.

    abstract:BACKGROUND:The Institute of Medicine, in a 2013 report, recommended that the Vaccine Safety Datalink (VSD) expand collaborations to include more diversity in the study population. Kaiser Permanente Colorado (KPCO), an established VSD site, partnered with Denver Health (DH), an integrated safety net healthcare system, t...


    pub_type: 杂志文章


    authors: Narwaney KJ,Breslin K,Ross CA,Shoup JA,Wain KF,Weintraub ES,McNeil MM,Hambidge SJ

    更新日期:2017-03-01 00:00:00

  • Integration of hepatitis B vaccination into the expanded programme on immunization in Chonburi and Chiangmai provinces, Thailand.

    abstract::Hepatitis B (HB) immunization was introduced as part of the expanded programme on immunization (EPI) in two provinces in Thailand and evaluated over a four year period. Three doses of HB vaccine were offered to 60,980 newborns at birth, 2 and 6 months of age. The overall coverage for complete HB immunization was 90.4%...


    pub_type: 杂志文章


    authors: Chunsuttiwat S,Biggs BA,Maynard J,Thamapalo S,Laoboripat S,Bovornsin S,Charanasri U,Pinyowiwat W,Kunasol P

    更新日期:1997-04-01 00:00:00

  • Complications of influenza and benefits of vaccination.

    abstract::Uncertainty regarding the benefits of influenza vaccination may contribute to the underutilization of this vaccine. We have conducted serial cohort studies using the administrative data bases of a Twin Cities based managed care organization to assess the impact of disease and benefits of vaccination among the elderly....


    pub_type: 杂志文章,多中心研究


    authors: Nichol KL

    更新日期:1999-07-30 00:00:00

  • Monitoring of rotavirus vaccination in Morocco: establishing the baseline burden of rotavirus disease.

    abstract:BACKGROUND:Rotavirus is a leading cause of childhood morbidity and mortality worldwide. Clinical trials for two rotavirus vaccines recommended by the WHO for global use since 2009 have successfully demonstrated the safety and efficacy of these vaccines in a wide range of countries. To control the burden of severe and f...


    pub_type: 杂志文章


    authors: Benhafid M,Rguig A,Trivedi T,Elqazoui M,Teleb N,Mouane N,Maltouf AF,Parashar U,Patel M,Aouad RE

    更新日期:2012-10-12 00:00:00

  • Lessons learned in the analysis of high-dimensional data in vaccinomics.

    abstract::The field of vaccinology is increasingly moving toward the generation, analysis, and modeling of extremely large and complex high-dimensional datasets. We have used data such as these in the development and advancement of the field of vaccinomics to enable prediction of vaccine responses and to develop new vaccine can...


    pub_type: 杂志文章


    authors: Oberg AL,McKinney BA,Schaid DJ,Pankratz VS,Kennedy RB,Poland GA

    更新日期:2015-09-29 00:00:00

  • Indicators to assess National Immunization Technical Advisory Groups (NITAGs).

    abstract::A National Immunization Technical Advisory Group (NITAG) is an expert advisory committee that provides evidence-based recommendations to the Ministry of Health (MoH) to guide immunization programs and policies. The World Health Organization (WHO), the Initiative for Supporting National Independent Immunization and Vac...


    pub_type: 杂志文章


    authors: Blau J,Sadr-Azodi N,Clementz M,Abeysinghe N,Cakmak N,Duclos P,Janusz C,Jauregui B,Mihigo R,Mosina L,Takashima Y,Senouci K

    更新日期:2013-05-28 00:00:00

  • A canine adenovirus type 2 vaccine vector confers protection against foot-and-mouth disease in guinea pigs.

    abstract::Vaccination is a key element in the control of foot-and-mouth disease (FMD). The majority of the antigenic sites that induce protective immune responses are localized on the FMD virus (FMDV) capsid that is formed by four virus-encoded structural proteins, VP1 to VP4. In the present study, recombinant canine adenovirus...


    pub_type: 杂志文章


    authors: De Vleeschauwer AR,Zhou X,Lefebvre DJ,Garnier A,Watier F,Pignon C,Lacour SA,Zientara S,Bakkali-Kassimi L,De Clercq K,Klonjkowski B

    更新日期:2018-04-12 00:00:00

  • Chapter 26: Innovative financing mechanisms to accelerate the introduction of HPV vaccines in developing countries.

    abstract::The costs of developing and producing new-generation vaccines have increased compared to many of the older, "traditional" vaccines because of new technologies and regulatory requirements. While the public sector often supports basic research costs, private manufacturers are usually responsible for the investments in p...


    pub_type: 杂志文章,评审


    authors: Batson A,Meheus F,Brooke S

    更新日期:2006-08-31 00:00:00

  • Influenza virus antigens conjugated with a synthetic polyelectrolyte: a novel model of vaccines.

    abstract::Haemagglutinin (HA), a mixture of haemagglutinin and neuraminidase (HA + NA), and matrix (M) protein were isolated from the influenza A virus and covalently coupled to a synthetic polyelectrolyte (P). A single injection into mice of the resultant conjugates (virogates) brought about efficient stimulation of the primar...


    pub_type: 杂志文章


    authors: Petrov RV,Khaitov RM,Zhdanov VM,Sinyakov MS,Norimov ASh,Nekrasov AV,Podchernyaeva RYa,Kharitonenkov IG,Shchipanova MV

    更新日期:1985-12-01 00:00:00