An investigation of biooxidation ability of Acidithiobacillus ferrooxidans using NMR relaxation measurement.

Abstract:

:NMR relaxation measurements can provide a simple means for understanding biological activity of cells in solution with known composition. It has the advantage that it is an in situ, non-intrusive technique, and the acquisition is fast. The iron oxidation ability of Acidithiobacillus ferrooxidans was investigated using NMR relaxation measurements. The transversal relaxation is characterized by a time constant, T₂, which is sensitive to the chemical environment. Fe³⁺ ion has more significant T₂ shortening than Fe²⁺ ion. In the presence of A. ferrooxidans in solutions containing Fe²⁺ ion, T₂ shortening was found with increasing time as the bacteria oxidize Fe²⁺ to Fe³⁺ ions. In the optimal growth medium, the bacteria concentration increased 80 times and high iron oxidation rate was found. In 10 mM K₂SO₄ medium, however, bacteria concentration remained almost unchanged and the iron oxidation rate was significantly lower.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Tan SN,Burgar I,Chen M

doi

10.1016/j.biortech.2011.06.088

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

9143-7

issue

19

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(11)00915-1

journal_volume

102

pub_type

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