Abstract：
:Although plasmacytoid dendritic cells (pDCs) are involved in HIV-1 pathogenesis, the precise mechanism of interaction between pDCs and HIV-1 in vivo is not clear. The conflicting reports in HIV-1-infected patients highlight the importance of studying the interaction between HIV-1 and pDCs in relevant in vivo models. The rag2/γC double knockout (DKO) mouse supports reconstitution of a functional human immune system in central and peripheral lymphoid organs. We report here that functional pDCs were developed in the BM and peripheral lymphoid organs in humanized DKO (DKO-hu) mice. We show that pDCs from both BM and spleen were activated and productively infected during early HIV infection. The activation level of pDCs correlated with that of CD4⁺ T-cell activation and apoptosis. Although CD4⁺ T cells were preferentially depleted, pDCs were maintained but functionally impaired in the BM and spleen of HIV-infected DKO-hu mice. We conclude that HIV-1 can efficiently infect, activate, and impair pDCs in the BM and spleen, in correlation with CD4⁺ T-cell depletion. The humanized mouse will serve as a relevant model to investigate the development and function of pDCs and their role during HIV-1 pathogenesis in vivo.
journal_name
Bloodjournal_title
Bloodauthors
Zhang L,Jiang Q,Li G,Jeffrey J,Kovalev GI,Su Ldoi
10.1182/blood-2011-01-331173subject
Has Abstractpub_date
2011-06-09 00:00:00pages
6184-92issue
23eissn
0006-4971issn
1528-0020pii
blood-2011-01-331173journal_volume
117pub_type
杂志文章相关文献
BLOOD文献大全abstract：:We previously showed that granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) stimulate the differentiation of human monocytes into two phenotypically distinct types of macrophages. However, in vivo, not only CSF but also many other cytokines are produced under va...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-11-15 00:00:00
abstract：:A 2-yr-old black girl presented with a thalassemic clinical picture and was found to have nearly 100% fetal hemoglobin in her red cells. Pedigree analysis indicated that she was a heterozygote for the hereditary persistence of fetal hemoglobin gene and for a beta O-thalassemia gene. A brother, who also had nearly 100%...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:More than 90% of both unfractionated and blast-enriched T cell-depleted mononuclear cells (MNC) from a patient with a morphologically and phenotypically unclassified acute leukemia expressed interleukin 2 receptors (IL2-R), whereas 50% unfractionated MNC displayed monocyte/myeloid- (My7, My9, and OKM1) and B cell-rest...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:The molecular determinants regulating the specification of human embryonic stem cells (hESCs) into hematopoietic cells remain elusive. HOXA9 plays a relevant role in leukemogenesis and hematopoiesis. It is highly expressed in hematopoietic stem and progenitor cells (HSPCs) and is downregulated upon differentiation. Ho...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2014-03-558825
更新日期：2014-11-13 00:00:00
abstract：:Primary myelofibrosis (PMF) is the most serious myeloproliferative disorder, characterized by clonal myeloproliferation associated with cytokine-mediated bone marrow stromal reaction including fibrosis and osteosclerosis. Current drug therapy remains mainly palliative. Because the NF-kappaB pathway is implicated in th...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-10-054502
更新日期：2007-07-01 00:00:00
abstract：:Malignant cells from 52 children with acute lymphocytic leukemia (ALL) were investigated for inactivation of the p15ink4B and p16ink4 genes and other genetic alterations on chromosome 9p21. Homozygous deletions of the p15ink4B and/or the p16ink4 genes were detected in 16 cases and a further 9 cases showed evidence of ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-06-15 00:00:00
abstract：:The expression of a number of blood coagulation factors (F) (FX, FIX, FVIII, FVII, alpha-, beta-, gamma-fibrinogen chains, protein C, and antithrombin III [AT III]) was analyzed at RNA and protein level in 5- to 10-week-old human embryos and fetuses. FX, FIX, and FVII were also analyzed at protein level. Total and pol...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-09-15 00:00:00
abstract：:In this issue of Blood, Usmani et al provide important information on second primary malignancies in patients treated with thalidomide and lenalidomide in the Arkansas total therapy trials. ...
journal_title：Blood
pub_type： 评论,杂志文章
doi：10.1182/blood-2012-07-439158
更新日期：2012-08-23 00:00:00
abstract：:HIV/AIDS has long been at the forefront of the development of gene- and cell-based therapies. Although conventional gene therapy approaches typically involve the addition of anti-HIV genes to cells using semirandomly integrating viral vectors, newer genome editing technologies based on engineered nucleases are now all...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2016-01-678144
更新日期：2016-05-26 00:00:00
abstract：:Ristocetin and vancomycin are structurally similar glycopeptide antibiotics. Both vancomycin and ristocetin in high concentrations (3.0 mg/ml) cause the precipitation of fibrinogen, plasminogen, and IgG from platelet-poor plasma (PPP). In contrast to ristocetin, vanomycin (0.5-1.5 mg/ml) does not agglutinate platelets...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Overexpression of transcription factors runt-related transcription factor 1 (RUNX1) and nuclear factor, erythroid-derived 2 (NF-E2) was reported in granulocytes of patients with polycythemia vera and other myeloproliferative neoplasms (MPNs). Further, a transgenic mouse overexpressing the NF-E2 transgene was reported ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2013-10-534222
更新日期：2014-01-16 00:00:00
abstract：:Telomere erosion may be counteracted by telomerase. Here we explored telomere length (TL) and telomerase activity (TA) in primary cutaneous T-cell lymphoma (CTCL) by using quantitative polymerase chain reaction and interphase quantitative fluorescence in situ hybridization assays. Samples from patients with Sézary syn...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2013-05-500686
更新日期：2014-03-20 00:00:00
abstract：:The molecular basis for the hereditary persistence of fetal hemoglobin (HPFH) phenotype was studied in a Chinese individual who was heterozygous for a nondeletion form of A gamma-HPFH. Both allelic A gamma-globin genes were isolated by molecular cloning and subjected to nucleotide sequence analysis. One A gamma gene p...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:To assess the relationship between venous thrombosis and plasma glucosylceramide (GlcCer) or phosphatidylethanolamine (PE), plasma levels of GlcCer and PE were determined for 70 venous thrombosis patients referred for evaluation and 70 healthy blood donors. The mean GlcCer level, but not the PE level, was lower in pat...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.v97.7.1907
更新日期：2001-04-01 00:00:00
abstract：:Light-mediated release of signaling ligands, such as chemoattractants, growth factors, and cytokines is an attractive strategy for investigation and therapeutic targeting of leukocyte communication and immune responses. We introduce a versatile optogenetic method to control ligand secretion, combining UV-conditioned e...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2015-11-684852
更新日期：2016-06-09 00:00:00
abstract：:Chemoresistance is a major problem in the treatment of patients with multiple myeloma (MM). Because of the central role of the nuclear transcription factors nuclear factor-kappaB (NF-kappaB) and signal transducer and activator of transcription 3 (STAT3) in chemoresistance, cell survival, and proliferation, we investig...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-06-2151
更新日期：2004-04-15 00:00:00
abstract：:For activation T cells engage antigen-presenting cells (APCs) in lymphatic tissues. The contact duration and kinetics (static versus dynamic) vary considerably in different model systems; however, it is unclear whether T cells, APCs, or the environment are responsible for the observed discrepancies. Using 3-D collagen...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-03-1193
更新日期：2004-11-01 00:00:00
abstract：:Outgrowth, long-term self-renewal, and terminal maturation of human erythroid progenitors derived from umbilical cord blood in serum-free medium can be modulated by steroid hormones. Homogeneous erythroid cultures, as characterized by flow cytometry and dependence on a specific mixture of physiologic proliferation fac...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-03-1002
更新日期：2005-01-01 00:00:00
abstract：:Three Rh-related cDNAs have been isolated from a human bone marrow cDNA library and by polymerase chain reaction (PCR) amplification of human bone marrow and erythroblast mRNAs. They potentially encode a family of Rh protein isoforms that exhibit several unexpected structural properties as compared with the Rh polypep...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-08-15 00:00:00
abstract：:The splenic marginal zone (MZ) is comprised of specialized populations of B cells, dendritic cells, and macrophages that are uniquely arrayed outside the white pulp follicles to screen the blood for bacterial and other particulate Ags. Mechanisms responsible for MZ B-cell formation, localization, retention, and functi...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-03-343608
更新日期：2012-01-12 00:00:00
abstract：:Fibrin is a hallmark of immune-mediated tissue lesions. The presence of fibrin in such lesions implies both the formation of fibrin via coagulation and the accompanying restriction of fibrinolysis, allowing fibrin to persist. Previous work has shown that human monocytes exposed to an inflammatory stimulus such as lipo...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Mast cells accumulate at sites of neovascularization, solid tumors, and many immune reactions. Such accumulation requires directed migration of mature mast cells or their precursors. The nature of the chemoattractants that regulate mast cell motility and the identity of the receptors that mediate the chemotactic respo...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-02-15 00:00:00
abstract：:Stem cell factor (SCF) binding to the c-kit receptor triggers homodimerization and intermolecular tyrosine phosphorylation of the c-kit receptor, thus initiating signal transduction. Receptor dimerization is a critical early step in this process. Prior biochemical studies of c-kit receptor dimerization have mainly use...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:A decrease in inhibitor titer has been reported in some patients with acquired factor VIII inhibitors treated with intravenous gammaglobulin (IGIV). We have conducted a prospective, multicenter study of high-dose IGIV in treatment of this disorder to determine efficacy. Nineteen patients received induction therapy wit...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-07-15 00:00:00
abstract：:The cellular immune defect in untreated Hodgkin's disease (HD) has long been recognized. This defect appears to be responsible for at least some of the morbidity and ultimately the mortality associated with the disease. In recent years, many studies have shown that the T cell component of the immune response is the ap...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:Stem cells of highly regenerative organs including blood are susceptible to endogenous DNA damage caused by both intrinsic and extrinsic stress. Response mechanisms to such stress equipped in hematopoietic stem cells (HSCs) are crucial in sustaining hematopoietic homeostasis but remain largely unknown. In this study, ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-01-330050
更新日期：2011-09-15 00:00:00
abstract：:In this issue of Blood, Jodele and colleagues report that defective complement regulation contributes to the development of thrombotic microangiopathy (TMA) after hematopoietic stem cell transplantation (HSCT) with important implications for diagnosis and management of this severe clinical complication. ...
journal_title：Blood
pub_type： 评论,杂志文章
doi：10.1182/blood-2013-07-512707
更新日期：2013-09-19 00:00:00
abstract：:BB-10010 is a variant of the human form of macrophage inflammatory protein-1alpha (MIP-1alpha), which has been shown in mice to block the entry of hematopoietic stem cells into S-phase and to increase their self-renewal capacity during recovery from cytotoxic damage. Its use may constitute a novel approach for protect...
journal_title：Blood
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1998-09-01 00:00:00
abstract：:Leukemic blasts from 774 children with newly diagnosed acute lymphocytic leukemia (ALL) have been phenotyped by microcytotoxicity testing with a panel of monoclonal antibodies and heteroantisera as part of a Pediatric Oncology Group classification study of acute leukemia. One hundred twenty-two cases, or 16% were desi...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Acquired chromosomal anomalies (most commonly translocations) in lymphoma and leukemia usually result in either activation of a quiescent gene (by means of immunoglobulin or T-cell-receptor promotors) and expression of an intact protein product, or creation of a fusion gene encoding a chimeric protein. This review sum...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood.v99.2.409
更新日期：2002-01-15 00:00:00
