Abstract：
:Light-mediated release of signaling ligands, such as chemoattractants, growth factors, and cytokines is an attractive strategy for investigation and therapeutic targeting of leukocyte communication and immune responses. We introduce a versatile optogenetic method to control ligand secretion, combining UV-conditioned endoplasmic reticulum-to-Golgi trafficking and a furin-processing step. As proof of principle, we achieved light-triggered chemokine secretion and demonstrated that a brief pulse of chemokine release can mediate a rapid flux of leukocyte contacts with target cells in vitro and in vivo. This approach opens new possibilities for dynamic investigation of leukocyte communication in vivo and may confer the potential to control the local release of soluble mediators in the context of immune cell therapies.
journal_name
Bloodjournal_title
Bloodauthors
Sarris M,Olekhnovitch R,Bousso Pdoi
10.1182/blood-2015-11-684852subject
Has Abstractpub_date
2016-06-09 00:00:00pages
e35-41issue
23eissn
0006-4971issn
1528-0020pii
blood-2015-11-684852journal_volume
127pub_type
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