Abstract:
:Factor IX (F.IX) is a vitamin K-dependent plasma protein, a deficiency of which results in hemophilia B. A canine model of hemophilia B exists; attempts to use this model for gene transfer experiments or characterization of the hemophilic defect require elucidation of normal canine F.IX structure. We report the isolation and characterization of the coding region for canine F.IX cDNA. Canine F.IX possesses 86% identity at the amino-acid level with human F.IX. The leader peptide, Gla domain, EGF domains, and the carboxy-terminal portion of the heavy chains show extensive sequence conservation between the canine and human. All Glu residues undergoing gamma-carboxylation in humans are conserved in canines. The complete coding sequence for canine F.IX has been determined, and the derived translation product has been analyzed. A similar approach should allow identification of the causative mutation in canine hemophilia B. Furthermore, this clone may prove a valuable resource in gene transfer experiments for this disease.
journal_name
Bloodjournal_title
Bloodauthors
Evans JP,Watzke HH,Ware JL,Stafford DW,High KAsubject
Has Abstractpub_date
1989-07-01 00:00:00pages
207-12issue
1eissn
0006-4971issn
1528-0020journal_volume
74pub_type
杂志文章相关文献
BLOOD文献大全abstract::Nitric oxide (NO), an important effector molecule involved in immune regulation and host defense, was shown to induce apoptosis in lymphoma cells. In the present report the NO donor glycerol trinitrate was found to induce apoptosis in Jurkat cells that are sensitive to CD95-mediated kill. In contrast, a CD95-resistant...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::High-molecular weight DNAs of fresh bone marrow cells from 32 patients with fresh leukemia were assayed for the presence of transmissible activated transforming genes by a DNA-mediated gene transfer technique using NIH/3T3 cells. DNAs of bone marrow cells from four of the 32 patients induced transformation of NIH/3T3 ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-12-01 00:00:00
abstract::Serial erythrocyte volume distribution curves were used to characterize changes in erythrocyte subpopulations following the induction of Heinz body hemolytic anemia in cats. Macrocytes produced in response to hemolysis were observed after loss of reticulum. After recovery from hemolysis, a gradual reduction in mean ce...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract::In ischemic stroke, treatment options are limited. Therapeutic thrombolysis is restricted to the first few hours after stroke, and the utility of current platelet aggregation inhibitors, including GPIIb/IIIa receptor antagonists, and anticoagulants is counterbalanced by the risk of intracerebral bleeding complications...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2008-04-144758
更新日期:2008-11-01 00:00:00
abstract::Platelet-von Willebrand factor (VWF) interactions must be tightly regulated in order to promote effective hemostasis and prevent occlusive thrombus formation. However, it is unclear what role the inherent properties of the bond formed between the platelet receptor glycoprotein Ibα and the A1 domain of VWF play in thes...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-04-569392
更新日期:2014-12-11 00:00:00
abstract::Although NPM1 gene mutations leading to aberrant cytoplasmic expression of nucleophosmin (NPMc(+)) are the most frequent genetic lesions in acute myeloid leukemia, there is yet no experimental model demonstrating their oncogenicity in vivo. We report the generation and characterization of a transgenic mouse model expr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-03-208587
更新日期:2010-04-22 00:00:00
abstract::Hemophilia B is a bleeding disorder caused by a deficiency of clotting factor IX (FIX). A colony of FIX deficient Lhasa Apso dogs has been established and the molecular basis of hemophilia B has been determined. The plasma factor IX levels were < 1% of normal canine levels in affected dogs. A complex deletion mutation...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::B-cell precursors are present in the thymus, and the thymic microenvironment is the source of lymphopoietic factors that include interleukin-7 (IL-7). Despite the fact that intrathymic B-cell progenitors are bone marrow-derived cells, the data in this report demonstrate that these progenitors accumulate at an early pr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-03-0733
更新日期:2002-11-15 00:00:00
abstract::Graft-versus-host disease (GVHD) remains one of the major complications after allogeneic bone marrow transplantation (allo-BMT). Sirtuin-1 (Sirt-1) plays a crucial role in various biological processes including cellular senescence, metabolism, and inflammatory responses. Sirt-1 deacetylation regulates different transc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-07-863233
更新日期:2019-01-17 00:00:00
abstract::Elevated circulatory levels of many blood coagulation factors are known to be a risk factor for deep vein thrombosis in humans. Here we report the first direct demonstration of a close association between elevated circulatory factor IX levels in mice with thrombosis as well as myocardial fibrosis. Transgenic mice over...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-05-1581
更新日期:2003-03-01 00:00:00
abstract::To elucidate the role of retinoic acid (RA) in anti-CD40 + interleukin-4 (IL-4)-mediated B-cell activation, the effect of 10(-12) to 10(-6) mol/L RA was studied in anti-CD40 (1 microgram/mL) + IL-4 (5 ng/mL)-mediated proliferation and Ig synthesis by human peripheral blood mononuclear cells (PBMC) and B cells in healt...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Deregulation of Notch signaling has been linked to the development of T-cell leukemias and several solid malignancies. Yet, it is unknown whether Notch signaling is involved in the pathogenesis of mycosis fungoides and Sézary syndrome, the most common subtypes of cutaneous T-cell lymphoma. By immunohistochemistry of 4...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-12-260216
更新日期:2010-10-07 00:00:00
abstract::Current approaches for treatment of late-stage breast cancer rarely result in a long-term cure. In part this is due to tumor stroma that prevents access of systemically or intratumorally applied therapeutics. We propose a stem cell gene therapy approach for controlled tumor stroma degradation that uses the pathophysio...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-10-187237
更新日期:2009-05-28 00:00:00
abstract::The mechanisms by which hematopoietic progenitor cells become lineage-committed remain poorly understood. A cloned subline of the AML14 cell line (AML14.3D10) that spontaneously differentiates to eosinophilic myelocytes in the absence of cytokine stimulation was obtained by limiting dilution. This subline exhibits aug...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-11-15 00:00:00
abstract::The presence of multiple VHDJH joinings in upwards of 30% of acute lymphoblastic leukemias (ALL) suggests a relative instability of the rearranged immunoglobulin heavy chain (IgH) gene, but the mechanisms involved are not completely understood. An investigation of the structure of the VHDJH joinings using complementar...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Over the past 5 years, many novel approaches to early diagnosis, prevention, and treatment of acute graft-versus-host disease (aGVHD) have been translated from the bench to the bedside. In this review, we highlight recent discoveries in the context of current aGVHD care. The most significant innovations that have alre...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2014-01-514786
更新日期:2014-07-17 00:00:00
abstract::A qualitative defect of antithrombin III (AT III) has been demonstrated over three generations in eight members of an Italian family by the discrepancy between a normal amount of antigen and decreased antithrombin and anti-Xa activity in the presence or in the absence of heparin. By two-dimensional immunoelectrophores...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Thymosin beta 4 (T beta 4) was originally isolated as a thymic hormone. Its functional properties remain obscure; however, the N-terminal peptidic sequence could have a regulatory function on hematopoietic stem cell proliferation. To investigate the mechanism of T beta 4 expression, we studied T beta 4 gene expression...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::Neutrophilic differentiation is dependent on CCAAT enhancer-binding protein α (C/EBPα), a transcription factor expressed in multiple organs including the bone marrow. Using functional genomic technologies in combination with clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-01-695759
更新日期:2016-06-16 00:00:00
abstract::The causes of anemia and immunosuppression, major outcomes of malaria, are not well established. This study was undertaken to investigate whether erythropoietin (EP) production is adequate and whether the hemopoietic stem cells (CFU-S) were affected during the course of infection. Groups of female Balb/c mice infected...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
abstract::Chronic granulomatous disease (CGD) is an inherited immunodeficiency resulting from the inability of an individual's phagocytes to produce superoxide anions because of defective NADPH oxidase. The disease may be treated by bone marrow transplantation and as such is a candidate for somatic gene therapy. Two thirds of p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::A mutant strain of Escherichia coli (E. coli ML-35) was used to follow the kinetics of phagocytosis, perforation of the bacterial cell envelope, and inactivation of bacterial proteins by human neutrophils. This particular E. coli mutant strain has no lactose permease, but constitutively forms the cytoplasmic enzyme be...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::Clonal chromosomal abnormalities in Philadelphia chromosome-negative (CCA/Ph-) metaphases emerge as patients with chronic phase chronic myeloid leukemia (CP-CML) are treated with tyrosine kinase inhibitors (TKIs). We assessed the characteristics and prognostic impact of 598 patients with CP-CML treated on clinical tri...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2017-07-792143
更新日期:2017-11-09 00:00:00
abstract::Lymphomas afflict all age groups of people, with certain types demonstrating a female predilection in adolescents and young adults. A proportion of lymphomas that are diagnosed in this population demographic occur in the setting of pregnancy. Most of these behave aggressively at presentation and require immediate or u...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019000961
更新日期:2020-11-05 00:00:00
abstract::Serotonin (5-hydroxytryptamine, or 5-HT), released from activated platelets, not only accelerates aggregation of platelets but also is known to promote mitosis, migration, and contraction of vascular smooth muscle cells (VSMCs). These effects are considered to contribute to thrombus formation and atherosclerosis. The ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.6.1697
更新日期:2001-03-15 00:00:00
abstract::Conventional chemotherapy has failed to substantially prolong survival for patients with advanced follicular lymphoma. To improve outcomes, the German Low-Grade Lymphoma Study Group (GLSG) initiated a randomized trial to compare the effect of potentially curative myeloablative radiochemotherapy followed by autologous ...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2004-03-0982
更新日期:2004-11-01 00:00:00
abstract::Enforced BCL-2 gene expression in leukemic cell lines suppresses apoptosis and confers resistance to anticancer drugs, but the clinical significance of increased BCL-2 protein levels in acute lymphoblastic leukemia (ALL) is unknown. Among 52 children with newly diagnosed ALL, BCL-2 expression in leukemic lymphoblasts ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Acquired chromosomal anomalies (most commonly translocations) in lymphoma and leukemia usually result in either activation of a quiescent gene (by means of immunoglobulin or T-cell-receptor promotors) and expression of an intact protein product, or creation of a fusion gene encoding a chimeric protein. This review sum...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood.v99.2.409
更新日期:2002-01-15 00:00:00
abstract::Bcr-abl fusion peptide-specific CD4+ T-lymphocyte clones have recently been shown to augment colony formation by chronic myelogenous leukemia (CML) cells in a bcr-abl type-specific and HLA class II-restricted manner without addition of exogenous antigen. These findings suggest that CML cells can naturally process and ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.5.1498
更新日期:2001-09-01 00:00:00
abstract::The most frequently recurring translocations in mucosa-associated lymphoid tissue (MALT) B-cell non-Hodgkin lymphoma, t(11;18)(q21;q21) and t(14;18)(q32; q21), lead to formation of an API2-MALT1 fusion or IgH-mediated MALT1 overexpression. Various approaches have implicated these proteins in nuclear factor kappaB (NF-...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2297
更新日期:2005-04-01 00:00:00