Photodamaging effects of porphyrins and chitosan on primary human keratinocytes and carcinoma cell cultures.


BACKGROUND:Photodynamic therapy (PDT) is a non-surgical method for treating non-melanoma skin cancer and precancerous lesions which involves the activation of a photosensitizer by visible light to produce activated oxygen species within target cells, resulting in the destruction of the latter. The present study evaluates the effect of PDT on primary normal and basal cell carcinoma cultures in vitro. METHODS:Primary human keratinocytes and carcinoma cell cultures were exposed to various concentrations of 5,10,15,20-tetra-(para-methoxyphenyl) porphyrin (TMP) and its zinc compound (Zn-TMP) for 24 hours, with or without chitosan, and then irradiated using a PDT lamp (630 nm, 6 J/cm(2)). The effects of PDT were assessed using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay and an immunocytochemical method with Annexin V-FITC for detecting apoptosis. RESULTS:Both tested substances, TMP and Zn-TMP, had a phototoxic effect on primary human carcinoma cell cultures in concentrations of 1-100 μg/ml, which positively correlated with the concentration of the photosensitizer. There was no phototoxic effect on primary keratinocytes, probably because of the preferential accumulation of photosensitizing substances in tumoral cells. Administration of chitosan in association with photosensitizing substances increased cell viability compared with photosensitizers alone, exerting a cytoprotective effect. CONCLUSIONS:The study demonstrates that the photodynamic activity of TMP and its metalloporphyrin derivative is limited to primary human carcinoma cells and suggests that these porphyrins could be efficiently used in PDT in vivo.


Int J Dermatol


Susan M,Baldea I,Senila S,Macovei V,Dreve S,Ion RM,Cosgarea R




Has Abstract


2011-03-01 00:00:00












  • Primary cutaneous cryptococcosis in Brazil: report of 11 cases in immunocompetent and immunosuppressed patients.

    abstract:BACKGROUND:Primary cutaneous cryptococcosis is an uncommon infectious disease caused by Cryptococcus neoformans or Cryptococcus gattii affecting immunosuppressed as well as immunocompetent patients. It is often misdiagnosed as it may mimic other cutaneous diseases. MATERIALS AND METHODS:We report a series of cases dia...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Marques SA,Bastazini I Jr,Martins AL,Barreto JA,Barbieri D'Elia MP,Lastória JC,Marques ME

    更新日期:2012-07-01 00:00:00

  • Antidesmoglein 1 and 3 antibodies in healthy subjects of a population in the Peruvian high amazon.

    abstract:BACKGROUND:The objective of this study was to determine the presence of anti-Dsg1 and Dsg3 antibodies in healthy subjects of the high Peruvian Amazon (Tuemal, Rodriguez de Mendoza province, department of Amazonas) to establish the theoretical presence of environmental factors or triggers in the area. MATERIALS AND MET...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Ramos W,Díaz J,Gutierrez EL,Lazarte JS,Bohnett MC,Ronceros G,Ortega-Loayza AG

    更新日期:2018-03-01 00:00:00

  • Antihistamines are superior to topical steroids in managing human immunodeficiency virus (HIV)-associated papular pruritic eruption.

    abstract::Papular pruritic eruption (PPE) is a frequent HIV-comorbidity in tropical countries. Because of constant itching and social stigma, effective treatment is highly valued. In our HIV cohort in rural Tanzania with 12% prevalence of PPE, we have retrospectively analyzed responses to available treatments. Oral promethazine...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Navarini AA,Stoeckle M,Navarini S,Mossdorf E,Jullu BS,Mchomvu R,Mbata M,Kibatala P,Tanner M,Hatz C,Schmid-Grendelmeier P

    更新日期:2010-01-01 00:00:00

  • alpha1-Antitrypsin deficiency presenting with panniculitis and incidental discovery of chronic obstructive pulmonary disease.

    abstract::A 60-year-old man presented to the Emergency Department (ED) with large, painful, indurated plaques on the right thigh, left abdomen, left chest, and right chest, which began without any preceding trauma on the right thigh 3 weeks prior to presentation in the ED. He was initially treated with cefazolin 1 g three times...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Korver G,Liu C,Petersen M

    更新日期:2007-10-01 00:00:00

  • Self-reported tattoo reactions in a cohort of 448 French tattooists.

    abstract:BACKGROUND:Self-reported complaints in people having tattoos, including swelling, itch, and photosensitivity, are frequent. Tattooists are usually heavily tattooed with multiple extended colored tattoos and constitute a specific population of interest. OBJECTIVES:To assess the prevalence of cutaneous complications on ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Kluger N

    更新日期:2016-07-01 00:00:00

  • Clinicodermoscopic study of histoid leprosy: a case series.

    abstract:BACKGROUND:Histoid leprosy (HL) is a highly bacilliferous form of leprosy which requires early diagnosis and management. Dermoscopy is a noninvasive diagnostic method for various malignant and nonmalignant disorders. The aim of this study was to describe the dermoscopic patterns of various types of HL lesions over diff...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Acharya P,Mathur MC

    更新日期:2020-03-01 00:00:00

  • Topical zinc therapy for acne vulgaris.

    abstract::A double-blind study of 30 patients with mild to moderate acne vulgaris was conducted to evaluate the efficacy of a topically applied 2% zinc sulfate solution for acne therapy. Over a 12-week period, no difference was noted between placebo- and zinc-treated participants in regard to either the number or type of acne l...

    journal_title:International journal of dermatology

    pub_type: 临床试验,杂志文章


    authors: Cochran RJ,Tucker SB,Flannigan SA

    更新日期:1985-04-01 00:00:00

  • Multiple cutaneous lymphoproliferative disorders showing a retained tumor clone by T-cell receptor gene rearrangement analysis: a case series of four patients and review of the literature.

    abstract:BACKGROUND:Mycosis fungoides (MF) is the most common primary cutaneous T-cell lymphoma (CTCL), followed by CD30+ lymphoproliferative disorders, including lymphomatoid papulosis (LyP) and primary cutaneous anaplastic large cell lymphoma (pcALCL). The objective was to report on a series of patients with different types o...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Stowman AM,Hsia LL,Kanner WA,Mahadevan MS,Bullock GC,Patterson JW

    更新日期:2016-02-01 00:00:00

  • Diffuse dermal angiomatosis of the breast: clinical and histopathological features.

    abstract:INTRODUCTION:Diffuse dermal angiomatosis is an entity in the spectrum of reactive angiomatoses characterized by erythematous plaques that mainly affect the lower extremities of patients with a personal history of peripheral vascular disease. Involvement of the breast is a rare event that has only been described as sing...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Sanz-Motilva V,Martorell-Calatayud A,Rongioletti F,Escutia-Muñoz B,López-Gómez S,Rodríguez-Peralto JL,Vanaclocha F

    更新日期:2014-04-01 00:00:00

  • Skin disorders in elderly subjects.

    abstract:BACKGROUND:Skin problems in the elderly population have rarely been the subject of scientific research. OBJECTIVES:The present study aimed to determine the prevalences of the most common dermatological conditions in elderly subjects and to investigate their associations with systemic diseases. METHODS:The study invol...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Reszke R,Pełka D,Walasek A,Machaj Z,Reich A

    更新日期:2015-09-01 00:00:00

  • The antiproliferative function of violacein-like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5-2 in UV-induced 2237 fibrosarcoma.

    abstract:BACKGROUND:In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5-2), against murine UV-induced 2237 fibrosarcoma and B16F10 melanoma cells. METHODS:The 2237, B16F10, C50, and NIH3T3 cel...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Mojib N,Nasti TH,Andersen DT,Attigada VR,Hoover RB,Yusuf N,Bej AK

    更新日期:2011-10-01 00:00:00

  • Characterization of circulating lymphocytes by monoclonal antibodies in childhood and adult leprosy.

    abstract::Peripheral blood lymphocyte assays using monoclonal antibodies were done in 66 patients with leprosy, consisting of 25 children and 41 adults. The results were statistically analyzed for correlations, if any, among the different age groups and matched controls. The results, however, failed to show any significant corr...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Sehgal VN,Chaudhry A,Sharma VK,Gupta CK

    更新日期:1991-11-01 00:00:00

  • Cutis marmorata telangiectatica congenita.

    abstract::The authors describe ten cases of cutis marmorata telangiectatica congenita (CMTC). All of these cases were sporadic. The incidence of associated anomalies was relatively low, and three patients had skin manifestations (cutaneous atrophy and nevus telangiectaticus). In two patients, the right leg was shorter and thinn...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Kennedy C,Oranje AP,Keizer K,van den Heuvel MM,Catsman-Berrevoets CE

    更新日期:1992-04-01 00:00:00

  • Is pemphigus herpetiformis an entity?

    abstract::Clinical, histologic, and immunofluorescence studies were performed in 15 patients with pemphigus herpetiformis. The initial diagnosis was dermatitis herpetiformis, IgA linear bullous dermatosis or bullous pemphigoid. The histology varied depending on the character of skin lesions, and showed eosinophilic spongiosis o...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Maciejowska E,Jablonska S,Chorzelski T

    更新日期:1987-11-01 00:00:00

  • A comparative study of topical 5% cysteamine versus 4% hydroquinone in the treatment of facial melasma in women.

    abstract:OBJECTIVE:To assess the efficacy and safety of topical 5% cysteamine versus 4% hydroquinone in the treatment of facial melasma in women. Topical 5% cysteamine is an antioxidant and tyrosinase inhibitor that has been shown to be effective in the treatment of melasma. However, to date, no study has compared the performan...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Lima PB,Dias JAF,Cassiano D,Esposito ACC,Bagatin E,Miot LDB,Miot HA

    更新日期:2020-12-01 00:00:00

  • A comparison of anti-desmoglein antibodies and indirect immunofluorescence in the serodiagnosis of pemphigus vulgaris.

    abstract:BACKGROUND:Indirect immunofluorescence (IIF) is the standard method for the detection of pemphigus autoantibodies. Commercially available enzyme-linked immunosorbent assays (ELISAs) have recently become available to measure serum antibodies (Abs) against desmoglein1 (Dsg1) and desmoglein3 (Dsg3). It has been suggested ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Zagorodniuk I,Weltfriend S,Shtruminger L,Sprecher E,Kogan O,Pollack S,Bergman R

    更新日期:2005-07-01 00:00:00

  • Severe human urticaria produced by ant (Odontomachus bauri, Emery 1892) (Hymenoptera: Formicidae) venom.

    abstract:BACKGROUND:Ant sting reactions are becoming an increasing problem in tropical countries. It is important for physicians to be aware of their possible cutaneous and systemic manifestations. METHODS:A child with a severe allergic reaction to an ant sting was examined and is discussed. This is the first case described in...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Rodriguez-Acosta A,Reyes-Lugo M

    更新日期:2002-11-01 00:00:00

  • Allergic reactions to insulin.

    abstract::Allergic reactions to insulin, although very common, are usually mild and self-limited. Many persistent effects can be corrected by simple means available to the discerning physician. In a few patients, however, they are severe and cause extreme morbidity or even mortality. These patients need to be recognized promptl...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Jegasothy BV

    更新日期:1980-04-01 00:00:00

  • Treatment of psoriasis with topical nitrogen mustard.

    abstract::With the advent of various new methods of treating psoriasis, it is necessary to remind physicians to consider an agent that is easy to administer, locally effective, relatively inexpensive, not time-consuming and without systemic toxic effects. Nineteen patients were treated with topical nitrogen mustard between 1972...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Handler RM,Medansky RS

    更新日期:1979-11-01 00:00:00

  • Acquired hypertrichosis lanuginosa.

    abstract::A 32-year-old Thai woman developed acquired hypertrichosis lanuginosa with generalized lanugo hair, deeply furrowed tongue, and keratosis pilaris. She had metastatic adenocarcinoma of the liver. A review of world literature reveals 24 cases of which 22 were associated with proven malignancies. Acquired hypertrichosis ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Sindhuphak W,Vibhagool A

    更新日期:1982-12-01 00:00:00

  • A recombinant human hyaluronidase sustained release gel for the treatment of post-surgical edema.

    abstract:BACKGROUND:Edema commonly accompanies surgical procedures and when excessive, can adversely affect surgical outcomes. The skin extracellular matrix, including one of its primary components, hyaluronan (HA), is a significant barrier to effective drainage of accumulated edematous fluid. Recombinant human hyaluronidase (r...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Nekoroski T,Paladini RD,Sauder DN,Frost GI,Keller GA

    更新日期:2014-06-01 00:00:00

  • Relapsing polychondritis, an underestimated dermatological urgency: case report and literature review.

    abstract:BACKGROUND:Relapsing polychondritis is an autoimmune multisystemic disease with primary chondral involvement. Its high mortality and morbidity make it a real clinical challenge. CASE DESCRIPTION:A 32-year-old woman with a history of relapsing polychondritis, refractory to multiple treatments, with multisystem compromi...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Cuestas D,Peñaranda E,Mora S,Cortes C,Galvis I,Patiño M,Velasquez O

    更新日期:2017-12-01 00:00:00

  • Effect of topical paromomycin on cell-mediated immunity during cutaneous leishmaniasis.

    abstract:BACKGROUND:The aim of this study was to evaluate the effect of topical paromomycin treatment on the development of immunity during cutaneous leishmaniasis. METHODS:Three parameters of immunity were measured in the course of the disease: leishmanicidal effector activity, lymphocyte proliferation (cell-mediated immunity...

    journal_title:International journal of dermatology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Frankenburg S,Gross A,Jonas F,Klaus S

    更新日期:1993-01-01 00:00:00

  • Grover disease: review of subtypes with a focus on management options.

    abstract::Grover disease (GD) is a benign eruption that causes a papulovesicular rash on the trunk and proximal extremities. It often resolves spontaneously but can follow a more chronic and fluctuating course that may last several years. Although the etiology remains unknown, several associated triggers have been identified in...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Aldana PC,Khachemoune A

    更新日期:2020-05-01 00:00:00

  • Clinicoepidemiological features of mycosis fungoides in Kuwait, 1991-2006.

    abstract:BACKGROUND:Mycosis fungoides (MF) is an indolent, most common type of cutaneous T-cell lymphoma (CTCL) with an average estimated incidence of 0.5 cases per 100,000 persons per year in the western world. Although various clinical and epidemiological features are well delineated in the western population, the data is sca...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Alsaleh QA,Nanda A,Al-Ajmi H,Al-Sabah H,Elkashlan M,Al-Shemmari S,Demierre MF

    更新日期:2010-12-01 00:00:00

  • The prevalence of pediculosis capitis in schoolchildren in Mersin, Turkey.

    abstract:BACKGROUND:Pediculosis capitis is an endemic parasitosis affecting many countries of the world. The aim of this study was to investigate the incidence of head lice infestation in Mersin, Turkey. METHODS:A total of 5318 elementary schoolchildren, aged 8-16 years, were examined for the presence of Pediculus capitis. If ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Kokturk A,Baz K,Bugdayci R,Sasmaz T,Tursen U,Kaya TI,Ikizoglu G

    更新日期:2003-09-01 00:00:00

  • Malignant hemangioendothelioma.

    abstract:BACKGROUND:The administration of interleukin-2 (IL-2) has recently been reported to be favorable for treating malignant hemangioendothelioma (MHE). METHODS:Two patients with MHE responded well to intralesional injections of recombinant IL-2 (rIL-2) without major side effects. The purpose of this study was to character...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Ihda H,Tokura Y,Fushimi M,Yokote R,Hashizume H,Shirahama S,Iwatsuki K,Murakami K,Takigawa M

    更新日期:1995-11-01 00:00:00

  • Senile erythroderma with serum hyper IgE.

    abstract::Eight patients with senile erythroderma with serum hyper IgE were studied. Characteristic laboratory findings were persistently elevated IgE levels, high peripheral-blood eosinophils, and high lactic dehydrogenase (LDH). Immunologic analysis using immunoglobulin antibodies showed frozen sections of inguinal lymph node...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Asai T,Horiuchi Y

    更新日期:1989-05-01 00:00:00

  • Netherton syndrome: a case report and review of the literature.

    abstract::Netherton syndrome is a rare disorder inherited in an autosomal recessive pattern consisting of ichthyosiform dermatosis, hair shaft abnormalities (trichorrhexis invaginata), and an atopic diathesis. Patients with Netherton syndrome have been found to have a mutation on chromosome 5q32 in a gene named SPINK5 (serine p...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Sun JD,Linden KG

    更新日期:2006-06-01 00:00:00

  • Comèl-Netherton syndrome and peeling skin syndrome type B: overlapping syndromes or one entity?

    abstract:BACKGROUND:A 42-year-old male patient suffering from the rare Comèl-Netherton syndrome is reported, and the symptoms are discussed together with the results of light and electron microscopic examinations. CASE REPORT:Our case presented peeling skin syndrome type B with spontaneous, lifelong skin shedding with underlyi...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Sárdy M,Fáy A,Kárpáti S,Horváth A

    更新日期:2002-05-01 00:00:00