Photodamaging effects of porphyrins and chitosan on primary human keratinocytes and carcinoma cell cultures.


BACKGROUND:Photodynamic therapy (PDT) is a non-surgical method for treating non-melanoma skin cancer and precancerous lesions which involves the activation of a photosensitizer by visible light to produce activated oxygen species within target cells, resulting in the destruction of the latter. The present study evaluates the effect of PDT on primary normal and basal cell carcinoma cultures in vitro. METHODS:Primary human keratinocytes and carcinoma cell cultures were exposed to various concentrations of 5,10,15,20-tetra-(para-methoxyphenyl) porphyrin (TMP) and its zinc compound (Zn-TMP) for 24 hours, with or without chitosan, and then irradiated using a PDT lamp (630 nm, 6 J/cm(2)). The effects of PDT were assessed using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay and an immunocytochemical method with Annexin V-FITC for detecting apoptosis. RESULTS:Both tested substances, TMP and Zn-TMP, had a phototoxic effect on primary human carcinoma cell cultures in concentrations of 1-100 μg/ml, which positively correlated with the concentration of the photosensitizer. There was no phototoxic effect on primary keratinocytes, probably because of the preferential accumulation of photosensitizing substances in tumoral cells. Administration of chitosan in association with photosensitizing substances increased cell viability compared with photosensitizers alone, exerting a cytoprotective effect. CONCLUSIONS:The study demonstrates that the photodynamic activity of TMP and its metalloporphyrin derivative is limited to primary human carcinoma cells and suggests that these porphyrins could be efficiently used in PDT in vivo.


Int J Dermatol


Susan M,Baldea I,Senila S,Macovei V,Dreve S,Ion RM,Cosgarea R




Has Abstract


2011-03-01 00:00:00












  • Antidesmoglein 1 and 3 antibodies in healthy subjects of a population in the Peruvian high amazon.

    abstract:BACKGROUND:The objective of this study was to determine the presence of anti-Dsg1 and Dsg3 antibodies in healthy subjects of the high Peruvian Amazon (Tuemal, Rodriguez de Mendoza province, department of Amazonas) to establish the theoretical presence of environmental factors or triggers in the area. MATERIALS AND MET...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Ramos W,Díaz J,Gutierrez EL,Lazarte JS,Bohnett MC,Ronceros G,Ortega-Loayza AG

    更新日期:2018-03-01 00:00:00

  • PTCH1 gene mutations in exon 17 and loss of heterozygosity on D9S180 microsatellite in sporadic and inherited human basal cell carcinomas.

    abstract:BACKGROUND:Basal cell carcinomas (BCCs) are the most frequent human cancer that results from malignant transformation of basal cells in the epidermis. Gorlin syndrome is a rare inherited autosomal dominant disease that predisposes with multiple BCCs and other birth defects. Both sporadic and inherited BCCs are associat...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Santos DC,Zaphiropoulos PG,Neto CF,Pimentel ER,Sanches JA Jr,Ruiz IR

    更新日期:2011-07-01 00:00:00

  • CO2 laser therapy in a case of steatocystoma multiplex with prominent nodules on the face and neck.

    abstract:BACKGROUND:Steatocystoma multiplex is an uncommon disorder which usually begins in adolescence or early adult life. The condition can be hereditary, as an autosomal dominant trait, or nonhereditary, as in this case. METHODS:A 40-year-old woman presented with a history of asymptomatic nodules that began around puberty ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Rossi R,Cappugi P,Battini M,Mavilia L,Campolmi P

    更新日期:2003-04-01 00:00:00

  • Prematurity: is it a risk factor for striae distensae?

    abstract:BACKGROUND:Although the causes of striae distensae (SD) remain to be elucidated, the condition is known to relate to changes in the structures that provide the skin with its tensile strength and elasticity. OBJECTIVE:This study was conducted to evaluate whether premature birth is a risk factor for SD. METHODS:A total...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Kelekci KH,Kelekci S,Destegul E,Aksoy A,Sut N,Yilmaz B

    更新日期:2011-10-01 00:00:00

  • The antiproliferative function of violacein-like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5-2 in UV-induced 2237 fibrosarcoma.

    abstract:BACKGROUND:In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5-2), against murine UV-induced 2237 fibrosarcoma and B16F10 melanoma cells. METHODS:The 2237, B16F10, C50, and NIH3T3 cel...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Mojib N,Nasti TH,Andersen DT,Attigada VR,Hoover RB,Yusuf N,Bej AK

    更新日期:2011-10-01 00:00:00

  • Features of dermatofibroma in reflectance confocal microscopy.

    abstract:BACKGROUND:Dermatofibroma (DF) is a common benign skin lesion in a majority of cases located on the legs or upper limbs. The etiology of DF is still unclear. OBJECTIVES:Reflectance confocal microscopy features of DF were described. METHODS:Forty patients with DF diagnosis confirmed by dermoscopy were examined using r...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Pogorzelska-Antkowiak A,Wcisło-Dziadecka D,Brzezińska-Wcisło L,Pawlicki K,Antkowiak R,Corneli P

    更新日期:2020-08-01 00:00:00

  • The combination of overweight and smoking increases the severity of androgenetic alopecia.

    abstract:INTRODUCTION:Even though androgenetic alopecia (AGA) is the most common type of alopecia, factors associated with AGA severity have been poorly investigated. OBJECTIVES:The objective of our study was to investigate risk factors for AGA severity among a Caucasian population. METHODS:A cross-sectional study was conduct...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Fortes C,Mastroeni S,Mannooranparampil TJ,Ribuffo M

    更新日期:2017-08-01 00:00:00

  • CD8+ cytotoxic mycosis fungoides: a retrospective analysis of clinical features and follow-up results of 29 patients.

    abstract:BACKGROUND AND OBJECTIVE:Less than 5% of cases of mycosis fungoides (MF) present with a cytotoxic/suppressor CD8+ phenotype. This study aimed to evaluate the clinical characteristics, treatment modalities, and clinical course in CD8+ MF patients. METHODS:In a retrospective analysis of 353 MF patients in a referral cen...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Kalay Yildizhan I,Sanli H,Akay BN,Sürgün E,Heper A

    更新日期:2020-01-01 00:00:00

  • Tranexamic acid accelerates skin barrier recovery and upregulates occludin in damaged skin.

    abstract:BACKGROUND:Tranexamic acid (TA) is a traditional plasmin inhibitor, and its role in the renovation of damaged skin has become the topic of a lot of research. The aim of this study is to determine whether TA could repair the skin barrier by means of tight intercellular junctions. METHODS:Two kinds of damaged skin model...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Yuan C,Wang XM,Yang LJ,Wu PL

    更新日期:2014-08-01 00:00:00

  • Clinicopathologic features of ulcerative-atrophic sarcoidosis.

    abstract:BACKGROUND:Sarcoidosis is a chronic granulomatous disease of unknown etiology. Cutaneous disease is common and includes two clinicopathologic categories: granulomatous infiltration or a reactive phenomenon. In the granulomatous infiltrative group, clinical manifestations can be variable. Ulcers in sarcoidosis are uncom...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Yoo SS,Mimouni D,Nikolskaia OV,Kouba DJ,Sauder DN,Nousari CH

    更新日期:2004-02-01 00:00:00

  • Azelaic acid (15% gel) in the treatment of acne rosacea.

    abstract::In December of 2002, the FDA approved azelaic acid 15% gel for the topical treatment of inflammatory papules and pustules of mild to moderate rosacea. Azelaic acid is a saturated dicarboxylic acid, which is naturally occurring and has been used in the treatment of rosacea, acne, and melasma. The 15% gel has a high eff...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Gupta AK,Gover MD

    更新日期:2007-05-01 00:00:00

  • Erosive pustular dermatosis of the scalp: causes and treatments.

    abstract::Erosive pustular dermatosis of the scalp is a rare condition which primarily affects older women after local trauma and has historically been treated with topical steroids. As it is a rare entity and resembles other dermatologic conditions, it may easily be misdiagnosed. Identifying the causes and evaluating the effic...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Karanfilian KM,Wassef C

    更新日期:2021-01-01 00:00:00

  • Prevalence and awareness of melasma during pregnancy.

    abstract:BACKGROUND:Melasma is an acquired hypermelanosis of the face. There is little information on its prevalence during pregnancy in Iran. Objective To determine the prevalence and awareness of melasma during pregnancy at the Shahed University Hospitals of Tehran, Iran. METHODS:A cross-sectional study was carried out by cl...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Moin A,Jabery Z,Fallah N

    更新日期:2006-03-01 00:00:00

  • Immunohistochemistry in histoid leprosy.

    abstract:BACKGROUND:Histoid leprosy is a rare form of multibacillary leprosy as the result of secondary or even primary resistance to dapsone. The etiopathogenesis has not been clarified up to now. METHODS:An immunohistochemical study was carried out for the expression of various markers on epidermal and dermal cell population...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Kontochristopoulos GJ,Aroni K,Panteleos DN,Tosca AD

    更新日期:1995-11-01 00:00:00

  • Chronic arsenic toxicity from Ayurvedic medicines.

    abstract:BACKGROUND:Ayurvedic medicines are known to contain arsenic and concentrations up to toxic levels have been reported in certain formulations. However, clinical disease due to arsenic containing ayurvedic medicines has rarely been reported. We seek to highlight the existence of toxic levels of arsenic in certain ayurved...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Khandpur S,Malhotra AK,Bhatia V,Gupta S,Sharma VK,Mishra R,Arora NK

    更新日期:2008-06-01 00:00:00

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    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Kawaguchi M,Mitsuhashi Y,Kondo S

    更新日期:2004-08-01 00:00:00

  • Measuring fatigue: a meta-review.

    abstract::There is a lack of validated tools to measure fatigue in patients with inflammatory skin, neuropsychiatric, and medical disorders. The use of nonvalidated tools may compromise the quality of data. The purpose of this meta-review was to evaluate existing fatigue scales commonly used to assess fatigue in other inflammat...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Machado MO,Kang NC,Tai F,Sambhi RDS,Berk M,Carvalho AF,Chada LP,Merola JF,Piguet V,Alavi A

    更新日期:2020-12-10 00:00:00

  • Absence of Merkel cells in lesional skin of vitiligo [corrected].

    abstract:BACKGROUND:In vitiligo, other than loss of melanocytes and melanin pigment in the lesional skin, keratinocytes are also involved. Human fetal Merkel cells are now generally considered to be derived from the epidermis. To date, no observations on Merkel cells in lesional skin of active vitiligo have been reported. METH...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Bose SK

    更新日期:1994-07-01 00:00:00

  • Prevalence of different skin conditions in an outpatients' setting in north-western Nigeria.

    abstract:BACKGROUND:Information on skin diseases in north-western Nigeria is scanty. We therefore conducted a prospective survey of 2611 new patients seen between August 1999 and July 2001 at the consultant medical/dermatology clinics of Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria. METHODS:The personal bio-d...

    journal_title:International journal of dermatology

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    authors: Onayemi O,Isezuo SA,Njoku CH

    更新日期:2005-01-01 00:00:00

  • Pityriasis in dermatology: an updated review.

    abstract::Dermatology has a very distinctive lexicon. The term pityriasis refers to several dermatologic conditions which all feature scaling of the skin. According to the Merriam-Webster dictionary, the term pityriasis was first used in print in 1684. Although the diseases beginning with the name pityriasis are of diverse caus...

    journal_title:International journal of dermatology

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    authors: Chin LD,Parvinnejad N,Haber RM

    更新日期:2020-08-11 00:00:00

  • Risks for noncutaneous second primary malignancy in cutaneous malignant melanoma survivors: an analysis of data from the Surveillance, Epidemiology, and End Results (SEER) program.

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    journal_title:International journal of dermatology

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    authors: Vakharia PP,Kelm RC,Orrell KA,Patel KR,Singam V,Ali Y,Rastogi S,Yousif R,Rangel SM,West DP,Nardone B

    更新日期:2020-04-01 00:00:00

  • Increased cutaneous fibrinolytic activity in a case of aquagenic pruritus.

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    authors: Lotti T,Cappugi P,Lattari P,Panconesi E

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  • International recommendations for an effective control of head louse infestations.

    abstract::Head louse infestations continue to be a concern of public health in most countries, including the most developed ones. The present recommendations are intended to inform and stress the role and impact of the different authorities, institutions, industry, and the public in the control of head lice in order to reduce t...

    journal_title:International journal of dermatology

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    authors: Mumcuoglu KY,Pollack RJ,Reed DL,Barker SC,Gordon S,Toloza AC,Picollo MI,Taylan-Ozkan A,Chosidow O,Habedank B,Ibarra J,Meinking TL,Vander Stichele RH

    更新日期:2020-08-07 00:00:00

  • Extramammary Paget's disease: a review of the literature.

    abstract::Extramammary Paget's disease (EMPD) is a rare cutaneous malignancy. The disease typically affects older individuals aged 60-80 years and is seen most frequently in postmenopausal Caucasian women and Asian men. EMPD exhibits a predilection for the genital and perianal regions and may be associated with an underlying ca...

    journal_title:International journal of dermatology

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    authors: Simonds RM,Segal RJ,Sharma A

    更新日期:2019-08-01 00:00:00

  • Prospective trial of curettage and cryosurgery in the management of non-facial, superficial, and minimally invasive basal and squamous cell carcinoma.

    abstract:BACKGROUND:As a variation of the curettage and destruction technique, cryosurgery can be used in place of electrodesiccation for the destructive component when managing superficial basal and squamous cell carcinomas. There are few studies, though, demonstrating the long-term cure rate associated with similar methods. T...

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    pub_type: 临床试验,杂志文章


    authors: Peikert JM

    更新日期:2011-09-01 00:00:00

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    authors: Laws PM,Heelan K,Al-Mohammedi F,Walsh S,Shear NH

    更新日期:2015-09-01 00:00:00

  • Squamous cell carcinoma in southern and northern Australia.

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    journal_title:International journal of dermatology

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    authors: Czarnecki D,Collins N,Meehan C,O'Brien T,Leahy S,Nash CA

    更新日期:1992-07-01 00:00:00

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    pub_type: 杂志文章


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    更新日期:2010-12-01 00:00:00