Abstract:
:Human urinary erythropoietin has been purified to homogeneity. The seven-step procedure yielded a preparation with a potency of 225,000 U/mg protein. SDS-polyacrylamide gel electrophoretic analysis of the purified hormone revealed a single protein band with a molecular weight of about 35,000 that migrated with the biological activity. As to its stability, the purified hormone retained its activity in the presence of 0.001% Tween 20.
journal_name
Acta Haematoljournal_title
Acta haematologicaauthors
Matsushita J,Kawakita M,Shibuya K,Koishihara Y,Sakaguchi M,Takatsuki Adoi
10.1159/000205057subject
Has Abstractpub_date
1990-01-01 00:00:00pages
169-74issue
4eissn
0001-5792issn
1421-9662journal_volume
84pub_type
杂志文章abstract::A 59-year-old man presented with lymphocytosis with huge splenomegaly. The abnormal lymphocytes had a high nucleoplasm:cytoplasm ratio, a prominent nucleolus and hairy cytoplasmic projections. Immunophenotyping revealed B-cell leukemia with negative reactions to CD5 and CD25. Cytogenetic study showed 46,XY,der(5)t(5;6...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000203984
更新日期:1995-01-01 00:00:00
abstract::A 56-year-old male presented with inguinal lymphadenopathy and leucocytosis (WBC 98 x 10(9)/l). Bone marrow morphology showed myeloid hyperplasia, with eosinophilia. Cytogenetic analysis showed no evidence of the Philadelphia chromosome, and fluorescence in situ hybridisation studies for the BCR/ABL fusion were negati...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000046638
更新日期:2002-01-01 00:00:00
abstract::All-trans-retinoic acid (ATRA) combined with anthracyclines is currently the standard treatment for acute promyelocytic leukemia (APL). In elderly patients the presence of comorbidities, such as cardiomyopathy or different organ failures, often represents an absolute contraindication to standard chemotherapy. In this ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000097880
更新日期:2007-01-01 00:00:00
abstract::A 69-year-old man with a rare case of lymphoma-type alpha-chain disease was admitted to the hospital with marked cervical and inguinal lymph node swelling. Lymph node biopsy showed marked infiltration of plasma cells, plasmacytoid cells and immunoblastoid cells, alone or in combination. Immunoelectrophoresis and immun...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204814
更新日期:1991-01-01 00:00:00
abstract::An ultrastructural study of the aspirated bone marrow of a patient with congenital dyserythropoietic anemia type I is presented. Both in transmission electron microscopy (TEM) and in scanning electron microscopy (SEM) ultrastructural abnormalities of the bone marrow erythroid precursors were seen. These abnormalities ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206735
更新日期:1983-01-01 00:00:00
abstract::A case of isolated lambda-light chain proteinemia and proteinuria is reported. During a 42-month follow-up, no sign of myelomatosis or amyloidosis developed and Bence Jones proteinuria remained nearly of the same magnitude. This case of apparently idiopathic Bence Jones gammopathy, the first lambda-type so far reporte...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207409
更新日期:1980-01-01 00:00:00
abstract::An increased bone marrow (BM) apoptosis is one of the mechanisms responsible for the ineffective hematopoiesis of myelodysplastic syndromes (MDS). It is controversial whether the excessive apoptosis in myelodysplasia predominantly involves the subset of progenitor cells or of maturing cells. We investigated the degree...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000067275
更新日期:2003-01-01 00:00:00
abstract::A patient with regional enteritis had received iron dextran for treatment of iron deficiency. Subsequently he developed a large (3.1 g/100 ml) IgG-K serum spike which had precipitin activity against dextran sulfate but not a variety of other antigens. There has been no evidence of multiple myeloma and the spike gradua...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207755
更新日期:1978-01-01 00:00:00
abstract::Essential thrombocythemia (ET), one of the chronic myeloproliferative disorders, is a clonal disorder of multipotent stem cells. Although most patients with ET have a prolonged benign course, a minority of patients may develop a blastic crisis similar to chronic myelogenous leukemia (CML). A case of ET terminating in ...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000204260
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Argininemia is an autosomal recessive urea cycle disorder (UCD). Unlike other UCD, hyperammonemia is rarely seen. Patients usually present in childhood with neurological symptoms. Uncommon presentations like neonatal cholestasis or cirrhosis have been reported. Although transient elevations of liver transami...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000493678
更新日期:2018-01-01 00:00:00
abstract::Acute megakaryoblastic leukemia is uncommon and comprises about 5% of acute nonlymphoid leukemias in the French-American-British classification. Cell lines from such leukemias are relatively rare with only about 8 reported in the literature. We established a cell line from a case of acute megakaryoblastic leukemia ari...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000203557
更新日期:1997-01-01 00:00:00
abstract::It was found that neutrophils in untreated uraemic patients as well as in subjects on regular dialysis treatment displayed higher activity of acid phosphatase, alkaline phosphatase and peroxidase. Spontaneous reduction of nitro blue tetrazolium (NBT) by granulocytes was also higher in both groups in comparison to cont...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207647
更新日期:1979-01-01 00:00:00
abstract::A case of lymphoma presenting with features shared by hairy cell leukemia (HCL) and its variant, intermediate lymphocytic lymphoma (ILL) and monocytoid B-cell lymphoma (MBCL) is described. Clinical presentation and the morphological findings observed in peripheral blood and in bone marrow biopsy suggested an HCL; howe...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204496
更新日期:1993-01-01 00:00:00
abstract::A 59-year-old man with beta-thalassaemia major is unusually well. He has no beta-chains in his haemoglobin but is heterozygous for the genes responsible for alphaA and for alphaG Philadelphia. In addition he is also heterozygous for the genes responsible for gammaF and a new gamma-chain, gamma75(E19) Ile-Thr, named ga...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000208204
更新日期:1975-01-01 00:00:00
abstract::A patient with common acute lymphoblastic leukaemia (ALL), hypereosinophilic syndrome and t(5;14) (q31.1;q32.3) translocation is described. Even with intensive treatment only short periods of complete remission were achieved. Recurrence of the leukaemia was always accompanied by the appearance of eosinophilic granuloc...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205289
更新日期:1989-01-01 00:00:00
abstract::High-dose gammaglobulin therapy for patients with idiopathic thrombocytopenic purpura (ITP), introduced by Imbach et al., was applied to 5 adults with chronic refractory ITP to investigate the mechanism of the increase in platelet counts. In 4 of the 5 cases, transient increase in platelet count was observed. Platelet...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206927
更新日期:1983-01-01 00:00:00
abstract::In the myelodysplastic syndromes (MDS) clonogenic marrow cell culture studies have demonstrated intrinsic hemopoietic stem cell and progenitor cell abnormalities consistent with these disorders representing clonal hemopathies. Abnormal responsiveness of these cells to stimulatory and inhibitory growth factors indicate...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000205911
更新日期:1987-01-01 00:00:00
abstract::Three consecutive patients considered to have end-stage acquired aplastic anaemia were given 100-160 mg/kg antilymphocyte globulin (ALG) i.v. followed by an infusion of 2-3.8 x 10(8) nucleated marrow cells/kg i.v. from HL-A one haplotype-identical, MLC-positive family donors. All patients showed autologous marrow reco...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000208005
更新日期:1976-01-01 00:00:00
abstract:OBJECTIVE:We investigated the prevalence of opportunistic infections in HIV-infected women according to transferrin (TF) phenotype. METHODS:We conducted a cross-sectional study among 200 HIV-positive women in the Butare University Teaching Hospital in Rwanda. TF phenotypes were determined using starch gel electrophore...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000338262
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Thalassemia is a group of hereditary hemoglobinopathies caused by decreased or absent synthesis of α and/or β globin chains. Studies have shown that hypercoagulability and thrombosis are common clinical symptoms in β-thalassemia, especially β-thalassemia intermedia, but little is known about in α-thalassemia...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000486157
更新日期:2018-01-01 00:00:00
abstract::Twenty-four infants and children suffering from glucose-6-phosphate dehydrogenase (G6PD) deficiency during hemolytic crisis were included in this study. Their ages ranged between 3 and 36 months with a median of 10 months. 22 were males and 2 were females. Fourteen out of them received a single bolus dose of desferrio...
journal_title:Acta haematologica
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000205320
更新日期:1989-01-01 00:00:00
abstract::A new case of IgE myeloma is described. A 77-year-old woman presented with bone pain and fatigue. Serum protein analysis revealed a paraprotein of the IgE kappa type; bone marrow aspirate and immunofluorescence confirmed the diagnosis; ultrastructural examination showed immature plasma cells. Treatment with prednisone...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204851
更新日期:1991-01-01 00:00:00
abstract::Pyrimidine 5'-nucleotidase (P5'N) partial deficiency has been described in several hematological disorders and also in the beta-thalassemic trait. To check if the P5'N deficiency in thalassemia was acquired we used thalassemic red cells (from either homo- or heterozygous subjects), whose P5'N activity was lower than i...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205286
更新日期:1989-01-01 00:00:00
abstract::Thrombopoietin levels in thrombocytopenic mice assayed by 75Se-se-lenomethionine incorporation into blood platelets reached a maximum 12 h after the induction of an acute, immune thrombocytopenia; that was more than twice the value in control mice. The implications of this finding are discussed with reference to the k...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000208096
更新日期:1975-01-01 00:00:00
abstract::Platelet aggregation induced in vitro with ADP, adrenalin and ristocetin was tested in 7 patients with megakaryoblastic leukemia (MKL). All patients had normal or high platelet counts and presented with hemorrhagic diathesis including purpura ecchymosis and epistaxis. Platelet morphology was grossly abnormal and elect...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206366
更新日期:1984-01-01 00:00:00
abstract::Recently, a factor was discovered in the serum of hepatectomized animals which was capable of augmenting the hepatic erythropoietin response to hypoxia when injected into normal rats. This substance was localized in the liver via an in situ perfusion technique and was termed the hepatic erythropoietic factor (HEF). Pa...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206884
更新日期:1983-01-01 00:00:00
abstract::A hairy-cell leukaemia (HCL) cell line, HCL-O, was established from the peripheral blood of a 62-year-old Japanese patient with a unique variant of HCL strongly expressing CD21, the receptor for the Epstein-Barr virus (EBV). The HCL-O cells expressed antigens similar and dissimilar to those expressed with the original...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204129
更新日期:1994-01-01 00:00:00
abstract::We have demonstrated the log normality of the distribution of sheep rosette-forming cells and mouse rosette-forming cells' values obtained with lymphocytes isolated from the peripheral blood of 135 healthy human beings and 57 patients suffering from chronic lymphocytic leukemia and well differentiated lymphocytic lymp...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207783
更新日期:1978-01-01 00:00:00
abstract::G-banded cytogenetic studies of 3 male patients in the terminal phase of chronic myeloid leukemia showed the following abnormalities: in the first case, the presence of a medullar cell line with 51 chromosomes and 3 Ph1; in the second case, a clone with 65 chromosomes and 4 Ph1, and in the third patient a clone with 5...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207047
更新日期:1982-01-01 00:00:00
abstract::Successive chromatographic procedures made it possible to isolate thrombocytopoietin from the serum of thrombocytopenic rats. The following steps were taken: DEAE-cellulose phosphate chromatography, Sephadex chromatography, exclusion chromatography on DEAE-Sephadex A-50 gel. The apparent molecular weight of thrombocyt...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206900
更新日期:1983-01-01 00:00:00