Abstract:
:Acute megakaryoblastic leukemia is uncommon and comprises about 5% of acute nonlymphoid leukemias in the French-American-British classification. Cell lines from such leukemias are relatively rare with only about 8 reported in the literature. We established a cell line from a case of acute megakaryoblastic leukemia arising in a 2-year-old child. Surface marker studies of the cells confirmed their megakaryoblastic nature, with 54% of the cells being CD61 positive and peroxidase and esterase negative. The cells had a doubling time of 72 h. Emperipolesis (a phenomenon in which a cell, usually a lymphocyte or neutrophil, enters another cell, moves about and leaves without undergoing phagocytosis) of one blast into another, larger one, was occasionally seen, and a review of the original bone marrow specimen also showed emperipolesis of neutrophils into the megakaryoblasts. The cells responded to interleukin 3 and were inhibited with all-trans-retinoic acid. The karyotype of the cells was the 46,XX,-16 with a marker chromosome. The marker chromosome is possibly chromosome 16 with a small segment of a chromosome translocated to the terminal portion of chromosome 16.
journal_name
Acta Haematoljournal_title
Acta haematologicaauthors
Skinnider LF,Pabello P,Zheng CYdoi
10.1159/000203557subject
Has Abstractpub_date
1997-01-01 00:00:00pages
26-31issue
1eissn
0001-5792issn
1421-9662journal_volume
98pub_type
杂志文章abstract::The expression of the enzyme marker terminal deoxynucleotidyl transferase (TdT) was examined by immunofluorescence assay in the cells from 333 cases with various types and subtypes of leukemia or lymphoma. More than 90% of cALL and T-ALL, 70% of Null-ALL and 80% of pre-B-ALL were TdT-positive. One case in the commonly...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206072
更新日期:1986-01-01 00:00:00
abstract::A 69-year-old woman presented with chronic lymphocytic leukemia (CLL) diagnosed after bone marrow recovery following intensive chemotherapy for acute lymphoblastic leukemia. The retrospective diagnosis of initial blastic transformation of CLL was made. The patient eventually died, 5 months after initial blastic phase,...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205751
更新日期:1988-01-01 00:00:00
abstract::A patient developed a drug rash and neutropenia while receiving tobramycin, ticarcillin and flucloxacillin intravenously for osteomyelitis. Incorporation of these antibiotics into in vitro cultures of bone marrow granulocyte macrophage precursors (CFU-C) showed no inhibition of the patient's marrow or normal marrow by...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206794
更新日期:1983-01-01 00:00:00
abstract::An ultrastructural study of the aspirated bone marrow of a patient with congenital dyserythropoietic anemia type I is presented. Both in transmission electron microscopy (TEM) and in scanning electron microscopy (SEM) ultrastructural abnormalities of the bone marrow erythroid precursors were seen. These abnormalities ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206735
更新日期:1983-01-01 00:00:00
abstract::We have used the gel retardation assay to investigate the binding of nuclear proteins to the duplicated CACCC boxes in the beta-globin gene promoter region. The effect of beta-thalassemia mutations affecting both of these consensus sequences (the -88 C-->T and -101 C-->T mutations) were studied by using appropriate mu...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204237
更新日期:1994-01-01 00:00:00
abstract::The dilution in the circulating blood of lymphocytes lablled in vitro with 3H-cytidine was examined after autotransfusion in 9 patients with Hodgkin's disease (HD) stage II A-IV B, 5 of whom were untreated; in 2 untreated patients with carcinoma, and in 1 treated patient with scleroderma. The blood transit time of exc...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000208185
更新日期:1975-01-01 00:00:00
abstract::The plasma cortisol levels of 108 children aged 1-16 years with various Hb genotypes (AA, AS and SS) were measured. The mean plasma cortisol levels of both the AS and SS groups (during steady state) fell within the normal range but the mean of the SS group is significantly lower than the AA group. In fact, 9 of the si...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205689
更新日期:1988-01-01 00:00:00
abstract::Red cell glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-chromosomal-linked abnormality often associated with hemolytic anemia. The G6PD variants obtained from 2 unrelated males, one associated with enzyme deficiency and history of hemolytic jaundice, and the other associated with enzyme deficiency but no ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206354
更新日期:1984-01-01 00:00:00
abstract::Recombinant alpha-interferons are used as therapeutic agents in an increasing number of benign and malignant disorders. Long-term administration of recombinant alpha-interferon as a maintenance agent is associated with a small number of adverse side-effects which are responsible for patient intolerance of this drug. T...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204881
更新日期:1991-01-01 00:00:00
abstract::Serum soluble interleukin-6 receptor (sIL-6R) concentrations were measured in 50 patients with plasma cell dyscrasias using a commercially available immunoenzymatic assay kit. There were 40 patients with multiple myeloma (MM), 5 patients with monoclonal gammopathy of undetermined significance (MGUS), 3 patients with s...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000203682
更新日期:1997-01-01 00:00:00
abstract::We studied neutrophil chemotaxis and surface membrane glycoproteins in 12 patients suffering from myeloid disorders with abnormal karyotype using in vitro techniques in all 12. Chromosome studies were also carried out virtually simultaneously. We chose to study only patients showing a deficit of chemotaxis (p less tha...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205543
更新日期:1989-01-01 00:00:00
abstract::Chronic pain affects 50% of adults with sickle cell disease (SCD). Although inflammation is thought to contribute to the pathogenesis of chronic pain, no studies have examined the differences in circulating cytokines between patients with SCD with and without chronic pain. We performed an observational cohort study us...
journal_title:Acta haematologica
pub_type: 新闻
doi:10.1159/000507563
更新日期:2021-01-01 00:00:00
abstract::6 patients with acute leukemia are described. All of them suffered from high temperature and malaise, and showed negative urine and blood cultures. High doses of gentamicin, cephalothin and cerebenicillin failed to lower the fever. The temperature became normal after administration of chloramphenicol (CAP) 2.0 g/day. ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207275
更新日期:1980-01-01 00:00:00
abstract::In a dose titration study we tested the efficacy and tolerance of recombinant human erythropoietin (rhEPO) in 10 patients with myelodysplasia (MDS) and 2 patients with idiopathic myelofibrosis. Patients with a haemoglobin level < 100 g/l were treated as out-patients for 12 weeks with daily doses ranging from 30 U/kg b...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204378
更新日期:1993-01-01 00:00:00
abstract::While sickle cell disease (SCD) is generally mild in most Kuwaitis, because of their elevated fetal Hb levels, avascular necrosis of the femoral head (AVNFH) appears to be a common complication. It was recently documented in 26.7% of Kuwaiti children with SCD. There have, however, been no previous studies of adult pat...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000072406
更新日期:2003-01-01 00:00:00
abstract::Non-transfusion-dependent thalassemia (NTDT) is associated with various forms of thalassemia and genetic modifiers. We report the molecular basis of NTDT in hemoglobin (Hb) E-β-thalassemia disease. This study was done in 73 adult patients encountered at the prenatal diagnosis center of Khon Kaen University, Northeast ...
journal_title:Acta haematologica
pub_type: 临床试验,杂志文章
doi:10.1159/000449120
更新日期:2016-01-01 00:00:00
abstract::Serological and biochemical studies were performed on M components isolated from 3 patients with transitional cell carcinoma of the urinary bladder (TCC). All were IgG1(K) proteins. 2 of the 3 belonged to the VKI subgroup, and these 2 also possessed cross-reactive idiotypic determinants. These findings are consistent ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206983
更新日期:1982-01-01 00:00:00
abstract::Cardiac scintigraphy has been performed in 60 beta-thalassaemia major patients aged 8-35 years who received regular blood transfusions and subcutaneous desferrioxamine (DFX) chelation. Fifty-seven showed no clinical, radiological or electrocardiographic evidence of heart disease and 3 had clinically apparent cardiac f...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205046
更新日期:1990-01-01 00:00:00
abstract::In the present study, we investigated the inhibitory natural killer cell receptor (NKR) expression of CD94/NKG2A on PBMC after allogeneic bone marrow transplantation (BMT). The proportion of CD94 expression on PBMC was higher in patients without chronic graft-versus-host disease (cGVHD) and also in cGVHD patients with...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000046540
更新日期:2001-01-01 00:00:00
abstract:INTRODUCTION:Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) down-regulation by preferentially expressed antigen of melanoma (PRAME) is a general phenomenon in different types of solid tumours, but research on the correlation between PRAME and TRAIL gene expression in leukaemia patients is rare. METHO...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000351166
更新日期:2013-01-01 00:00:00
abstract::Increased levels of hemoglobin A(2) (HbA(2)) are present in most beta-thalassemia carriers. The mechanism of this effect is not understood, although the increase may result from transcriptional and posttranscriptional changes. In the present study, we quantitate delta-globin mRNA levels in peripheral-blood-enriched re...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000040959
更新日期:1999-01-01 00:00:00
abstract::A case of lymphoma presenting with features shared by hairy cell leukemia (HCL) and its variant, intermediate lymphocytic lymphoma (ILL) and monocytoid B-cell lymphoma (MBCL) is described. Clinical presentation and the morphological findings observed in peripheral blood and in bone marrow biopsy suggested an HCL; howe...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204496
更新日期:1993-01-01 00:00:00
abstract::Two unrelated patients with Bernard-Soulier syndrome and their relatives were studied. The patients demonstrated severe bleeding diathesis, the relatives were asymptomatic. The propositi showed the characteristic abnormalities of the syndrome: thrombocytopenia, a percentage of giant platelets higher than 65%, prolonge...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206125
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:Posterior reversible encephalopathy syndrome (PRES) is a rare but serious complication after allogeneic hematopoietic stem cell transplantation (alloHSCT). Among others, calcineurin inhibitors (CNI) for prophylaxis of graft-versus-host disease (GvHD) may promote the development of PRES, but the pathomechanis...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000430489
更新日期:2016-01-01 00:00:00
abstract::A subpopulation with alkaline phosphatase activity and neutrophilic granules was found in leukemic monocytes from a child with acute monocytic leukemia (M5B). Almost all leukemic cells were strongly positive for nonspecific esterase and phagocytized opsonized zymosans. These findings suggest that the subpopulation are...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206516
更新日期:1984-01-01 00:00:00
abstract::350 patients with idiopathic thrombocytopenic purpura (ITP) aged 2/12-15 years (mean 6.3 +/- 2.7) were followed up during the period January 1st, 1975 to March 31, 1992. They constituted 40% of cases with hemorrhagic diathesis attending the Hematology/Oncology Clinic, Children's Hospital, Ain Shams University (relativ...
journal_title:Acta haematologica
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000204392
更新日期:1993-01-01 00:00:00
abstract::Pyrimidine 5'-nucleotidase (P5'N) partial deficiency has been described in several hematological disorders and also in the beta-thalassemic trait. To check if the P5'N deficiency in thalassemia was acquired we used thalassemic red cells (from either homo- or heterozygous subjects), whose P5'N activity was lower than i...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205286
更新日期:1989-01-01 00:00:00
abstract::The severe endothelial dysfunction in children with acute lymphoblastic leukemia (ALL) can result from the disease itself, from treatment, or from other conditions (e.g. sepsis). The aim of this study was to determine the levels of markers of endothelial activation in children with ALL and to assess their potential pr...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000322120
更新日期:2011-01-01 00:00:00
abstract::Contamination of autologous graft by tumor, in addition to incomplete tumor eradication, can partly explain why relapse remains the commonest cause of treatment failure after autologous stem cell transplantation (ASCT) in patients with malignant hematologic disorders. Monitoring of minimal residual disease (MRD) is no...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000088411
更新日期:2005-01-01 00:00:00
abstract::There have been many reports of patients with ampulla cardiomyopathy described as takotsubo-shaped cardiomyopathy in the cardiovascular field. This unique cardiomyopathy is characterized by transient apical ballooning and hypokinesis of the left ventricle. We describe 2 cases of ampulla cardiomyopathy associated with ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000098461
更新日期:2007-01-01 00:00:00