Abstract：
CONTEXT:Tumors of the head and neck commonly arise from the squamous and respiratory mucosa that lines the nasal and oral cavity, sinuses, pharynx, and larynx. The rate of oropharyngeal cancers diagnosed among Americans younger than 50 years is increasing. Infection of the oropharynx and tonsils by the human papillomavirus (HPV) has been linked to preneoplasia and cancer. OBJECTIVES:To evaluate the Roche Linear Array HPV Genotyping test kit to identify, and then specifically genotype, HPV in formalin-fixed, paraffin-embedded tissues. DESIGN:We evaluated the performance of this assay for accuracy, for intra-assay and interassay precision, and for its limit of detection, using materials with known HPV status. Sixteen tumor tissues with the following origins were evaluated: 1 ocular, 1 hypopharynx, 8 tonsil, 1 retromolar trigone, 3 tongue, 1 anal, and 1 lymph node. DNA from formalin-fixed, paraffin-embedded tumor sections was isolated and amplified in duplicate, with positive and negative controls, using primers specific to the polymorphic L1 region of the HPV genome. Thirty-seven genotypes were tested using the linear array. The amplified product (450 base pairs) was visualized by gel electrophoresis and, if positive, reflexed to HPV genotyping. RESULTS:Nine of the 16 tumors analyzed were HPV positive. The detected genotypes included HPV 6, 16, and 69. CONCLUSIONS:The Roche Linear Array HPV Genotyping test is an easy-to-use method for determining HPV genotype in the routine analysis of formalin-fixed, paraffin-embedded tumors. This assay is robust and can be performed routinely in a clinical laboratory setting.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Black CC,Bentley HA,Davis TH,Tsongalis GJdoi
10.1043/2009-0592-OAR.1subject
Has Abstractpub_date
2010-12-01 00:00:00pages
1813-7issue
12eissn
0003-9985issn
1543-2165pii
10.1043/2009-0592-OAR.1journal_volume
134pub_type
杂志文章abstract：:beta-Aminopropionitrile (BAPN) administered to rats has caused exostosis formation at sites of muscle attachment and also caused delay in the healing of soft tissue wounds and of bone fractures. Since phenytoin sodium has an opposite effect on wound healing, bone fractures, and the tensile strength of connective tissu...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:A case of tracheal neurilemoma that was removed by segmental tracheal resection was studied. Only seven other cases of benign neurogenic tracheal tumor (three neurofibromas and four neurilemomas) were found in the literature. Failure to consider this rare tracheal tumor as a cause of respiratory distress has frequentl...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:We encountered a rare case of pulmonary phaeohyphomycosis due to Xylohypha bantiana documented by culture. This dematiaceous (darkly pigmented) fungus is primarily neurotropic. It usually produces phaeohyphomycosis of the central nervous system but may also involve the skin and subcutaneous tissues. The patient, a 49-...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:We report the case of an adrenal cortical carcinoma with glandular and squamous differentiation, demonstrated by light and electron microscopy as well as by immunohistochemical studies. The patient was a 63-year-old man presenting with a large adrenal mass and markedly increased 24-hour urine metanephrine, initially s...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：CONTEXT:No study has evaluated radiology/pathology correlation of percentage viable tumor (PVT) estimates in ablated hepatocellular carcinoma (HCC) to examine the reliability of radiologic estimates. OBJECTIVE:To determine how well interdisciplinary PVT estimates correlate and identify pathologic factors that influenc...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2012-0126-OA
更新日期：2013-03-01 00:00:00
abstract：:Clinicopathologic studies of four patients with juvenile diabetes mellitus and renal disease demonstrated the pathogenetic variability of nephropathy in diabetic patients. Only in one patient was the clinical nephropathy associated with the typical diabetic glomerulosclerosis. Another patient had steroid responsive ne...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Trichomatricial carcinoma has been recently recognized as a rare malignant variant of pilomatricoma with a potential for local recurrence and, rarely, for distant metastases. We describe such a tumor in a 52-year-old man who developed local recurrence and axillary lymph node metastases within six months of local excis...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：CONTEXT:More than 25 years ago, John G. Azzopardi described a distinctive intraepithelial neoplastic breast lesion, which he designated "clinging carcinoma in situ"; he considered it as another variant of ductal carcinoma in situ, an early lesion that can easily be missed because the changes are mainly cytologic rather...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/1543-2165-133.6.879
更新日期：2009-06-01 00:00:00
abstract：CONTEXT:- Although many mesotheliomas are related to asbestos exposure, not all are, and there is increasing information on other causes of mesothelioma. OBJECTIVE:- To provide a review of non-asbestos causes for malignant mesothelioma. DATA SOURCES:- Review of relevant published literature via PubMed and other searc...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2017-0365-RA
更新日期：2018-06-01 00:00:00
abstract：:Accurate assessment of tissues for hematolymphoid neoplasms requires an integrated multiparameter approach. Although morphologic examination by light microscopy remains the mainstay of initial assessment for hematolymphoid neoplasms, immunophenotypic analysis by immunohistochemistry and/or flow cytometry is essential ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2016-0260-SA
更新日期：2017-03-01 00:00:00
abstract：CONTEXT:The rapid advances of computer technologies have created a new e-learner generation of "Homo-zappien" students that think and learn differently. Digital gaming is an effective, fun, active, and encouraging way of learning, providing immediate feedback and measurable process. Within the context of ongoing reform...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/2009-0698-OAR1.1
更新日期：2011-01-01 00:00:00
abstract：CONTEXT:- Intradepartmental consultation is a routine practice commonly used for new diagnoses. Expert interinstitutional case review provides insight into particularly challenging cases. OBJECTIVE:- To investigate the practice of breast pathology consultation at a large tertiary care center. DESIGN:- We reviewed bre...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2016-0371-OA
更新日期：2017-04-01 00:00:00
abstract：:A case of nongestational, extragonadal choriocarcinoma in a middle-aged man is described. Autopsy examination confirmed that the tumor originated in the lung. The trophoblastic origin of the tumor was documented by immunoperoxidase staining for human chorionic gonadotropin. ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:A 43-year-old white woman had a pheochromocytoma removed from her left adrenal gland, and one year later she developed a new left upper abdominal mass that was found to be a neuroblastoma. On both occasions, urinary vanillylmandelic acid level was elevated. However, urinary norepinephrine and epinephrine levels were i...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:A family with a syndrome consisting of granulomatous arthritis, uveitis, and rash was first described in 1985. Another family with the same syndrome was described subsequently in 1990. The condition, in both families, is clinically different from sarcoidosis. To verify whether the granulomas in such patients also coul...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:The costs of producing quality medical care and the proper allocation of resources to assure quality are major contemporary concerns. Minimalists attempt to improve quality and lower costs through the application of the theory, "less is more." Bureaucracies make the same attempt through the denial of payments for "unn...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:Tumoral calcinosis is a rare ectopic calcification syndrome characterized by irregular soft tissue masses originally described as being found mainly in large joints. We report 3 cases of tumoral calcinosis that occurred in unusual locations, the metacarpophalageal and proximal interphalangeal joints of the hand. The p...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2006)130[548:TCOTHT]2.0.CO;2
更新日期：2006-04-01 00:00:00
abstract：CONTEXT:Diagnosing epithelioid serosal lesions remains a challenge because numerous different processes-primary or secondary, benign or malignant-occur in body cavities, some of which are very rare. OBJECTIVES:To review the newest literature and to describe the morphologic criteria and immunohistochemical markers that...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2010-0216-RA
更新日期：2012-03-01 00:00:00
abstract：CONTEXT:Immunohistochemistry has become an indispensable ancillary study in the identification and classification of undifferentiated neoplasms/tumors of uncertain origin. The diagnostic accuracy has significantly improved because of the continuous discoveries of tissue-specific biomarkers and the development of effect...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2014-0061-RA
更新日期：2014-12-01 00:00:00
abstract：:The histopathologic characteristics of a patient with progressive myopathy, renal failure, hemoptysis, and a fulminant terminal event demonstrated a diffuse vasculitis, type 2 fiber atrophy of muscle, and linear IgG staining of the basement membranes of skeletal muscle fibers and of glomerular capillary basement membr...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:Mixed epithelial and stromal tumor of the kidney is a recently recognized distinct neoplasm that should be distinguished from other renal neoplasms. These tumors are relatively rare with a female preponderance. Imaging studies are not diagnostic but reveal a solid or solid and cystic mass in most cases. Histopathologi...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/1543-2165-133.9.1483
更新日期：2009-09-01 00:00:00
abstract：:Dissecting aneurysms of the pulmonary trunk are a rare cause of sudden death. We report a case in a patient with pulmonary hypertension secondary to a patent ductus arteriosus. A brief review of the condition is also presented. ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:We report a case of hepatic inflammatory myofibroblastic tumor in a 26-year-old African American man who presented with right upper quadrant pain, weight loss, and fatigue during the previous year. Hepatomegaly was found on physical examination. Laboratory findings were significant for mild normocytic, normochromic an...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2006)130[1548:IMTOTL]2.0.CO;2
更新日期：2006-10-01 00:00:00
abstract：:The alkaline guanidine method facilitates differentiation between different types of amyloid fibril proteins in formaldehyde-fixed, paraffin-embedded tissue sections. Systemic AA-type amyloids lost Congophilia (affinity of Congo red) after incubation with alkaline guanidine for one minute. Systemic AL-type amyloids lo...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:von Hippel-Lindau (VHL) disease is an autosomal dominant disorder caused by heterozygous mutations in the VHL tumor suppressor gene that is characterized by the occurrence of multiple endocrine and nonendocrine lesions. This review focuses on the endocrine manifestations of VHL disease. Pancreatic neuroendocrine proli...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2013-0520-RS
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:It has been suggested that localized Langerhans cell histiocytosis may represent an exaggerated form of a proliferative process that has been reported in a few patients with myasthenia gravis. To evaluate the relationship between thymic Langerhans' cell proliferation and myasthenia gravis, we analyzed a rare ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：CONTEXT:Obesity is associated with sleep disordered breathing and cardiovascular morbidity, but the relationship between pulmonary hypertension, heart disease, and obesity is unknown. OBJECTIVE:To determine the prevalence of pulmonary and cardiovascular disease in obese subjects undergoing autopsy at a large medical c...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2008)132[1397:PACCOO]2.0.CO;2
更新日期：2008-09-01 00:00:00
abstract：:Herein is presented a detailed description of the materials and methods employed in the conduct of an ongoing system for the independent evaluation (or testing) of individual sophomore medical students, as part of a major sophomore course in anatomic pathology. The academic environment and the advantages and disadvant...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：:Pathologic examination of an excisional biopsy specimen of a subepidermal nodule from the nasal tip of a 37-year-old black man demonstrated papillae covered by eosinophilic columnar epithelial cells, suggesting cylindric cell papilloma or well-differentiated papillary adenocarcinoma of nasal mucosa. The patient did no...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(2003)127
更新日期：2003-02-01 00:00:00
abstract：:A 9-year-old boy with metachromatic leukodystrophy (MLD) was found to have diffuse papillomatosis of the gallbladder that was virtually obliterating the lumen of the gallbladder. To our knowledge, two other examples of this lesion have been reported previously in MLD. ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
