Use of a linear array for the detection of human papillomavirus genotypes in head and neck cancer.

Abstract:

CONTEXT:Tumors of the head and neck commonly arise from the squamous and respiratory mucosa that lines the nasal and oral cavity, sinuses, pharynx, and larynx. The rate of oropharyngeal cancers diagnosed among Americans younger than 50 years is increasing. Infection of the oropharynx and tonsils by the human papillomavirus (HPV) has been linked to preneoplasia and cancer. OBJECTIVES:To evaluate the Roche Linear Array HPV Genotyping test kit to identify, and then specifically genotype, HPV in formalin-fixed, paraffin-embedded tissues. DESIGN:We evaluated the performance of this assay for accuracy, for intra-assay and interassay precision, and for its limit of detection, using materials with known HPV status. Sixteen tumor tissues with the following origins were evaluated: 1 ocular, 1 hypopharynx, 8 tonsil, 1 retromolar trigone, 3 tongue, 1 anal, and 1 lymph node. DNA from formalin-fixed, paraffin-embedded tumor sections was isolated and amplified in duplicate, with positive and negative controls, using primers specific to the polymorphic L1 region of the HPV genome. Thirty-seven genotypes were tested using the linear array. The amplified product (450 base pairs) was visualized by gel electrophoresis and, if positive, reflexed to HPV genotyping. RESULTS:Nine of the 16 tumors analyzed were HPV positive. The detected genotypes included HPV 6, 16, and 69. CONCLUSIONS:The Roche Linear Array HPV Genotyping test is an easy-to-use method for determining HPV genotype in the routine analysis of formalin-fixed, paraffin-embedded tumors. This assay is robust and can be performed routinely in a clinical laboratory setting.

journal_name

Arch Pathol Lab Med

authors

Black CC,Bentley HA,Davis TH,Tsongalis GJ

doi

10.1043/2009-0592-OAR.1

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1813-7

issue

12

eissn

0003-9985

issn

1543-2165

pii

10.1043/2009-0592-OAR.1

journal_volume

134

pub_type

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