Abstract：
CONTEXT:Tumors of the head and neck commonly arise from the squamous and respiratory mucosa that lines the nasal and oral cavity, sinuses, pharynx, and larynx. The rate of oropharyngeal cancers diagnosed among Americans younger than 50 years is increasing. Infection of the oropharynx and tonsils by the human papillomavirus (HPV) has been linked to preneoplasia and cancer. OBJECTIVES:To evaluate the Roche Linear Array HPV Genotyping test kit to identify, and then specifically genotype, HPV in formalin-fixed, paraffin-embedded tissues. DESIGN:We evaluated the performance of this assay for accuracy, for intra-assay and interassay precision, and for its limit of detection, using materials with known HPV status. Sixteen tumor tissues with the following origins were evaluated: 1 ocular, 1 hypopharynx, 8 tonsil, 1 retromolar trigone, 3 tongue, 1 anal, and 1 lymph node. DNA from formalin-fixed, paraffin-embedded tumor sections was isolated and amplified in duplicate, with positive and negative controls, using primers specific to the polymorphic L1 region of the HPV genome. Thirty-seven genotypes were tested using the linear array. The amplified product (450 base pairs) was visualized by gel electrophoresis and, if positive, reflexed to HPV genotyping. RESULTS:Nine of the 16 tumors analyzed were HPV positive. The detected genotypes included HPV 6, 16, and 69. CONCLUSIONS:The Roche Linear Array HPV Genotyping test is an easy-to-use method for determining HPV genotype in the routine analysis of formalin-fixed, paraffin-embedded tumors. This assay is robust and can be performed routinely in a clinical laboratory setting.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Black CC,Bentley HA,Davis TH,Tsongalis GJdoi
10.1043/2009-0592-OAR.1subject
Has Abstractpub_date
2010-12-01 00:00:00pages
1813-7issue
12eissn
0003-9985issn
1543-2165pii
10.1043/2009-0592-OAR.1journal_volume
134pub_type
杂志文章abstract：:"Gliding" contusions, ie, hemorrhagic lesions in the parasagittal white matter, were analyzed in 434 fatal nonmissile head injuries in humans. It is concluded that gliding contusions are a type of diffuse brain damage occurring at the moment of injury. Gliding contusions are significantly associated with road-traffic ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:We present the case of a 30-year-old woman who suddenly developed intense occipital headaches. Computed tomography and magnetic resonance imaging demonstrated a cyst located ventrally to the brain stem at the pontomedullary junction. The lesion was resected. Histologically, it was lined by a pseudostratified, ciliated...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：
更新日期：1992-02-01 00:00:00
abstract：CONTEXT:Lung cancer is the number one cause of cancer deaths in the United States and globally. The advent of targeted therapies has offered a new treatment paradigm for lung cancer, but currently validated and emerging drugs are effective in only a small minority of lung cancers, predominantly adenocarcinomas. Folate ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2012-0176-OA
更新日期：2013-02-01 00:00:00
abstract：:Over a 3-month period, 61,496 errors were detected in clinical laboratory reports by 631 participants in the College of American Pathologists' 1990 Q-Probes program. Each error detected was defined as an opportunity for improvement. Almost 4% of the detected errors were attributed to nonlaboratory personnel and approx...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:A retrospective study was performed to describe the histologic stages of selective myocardial cell necrosis (SMCN) in nonhuman primates, and to compare the incidence of SMCN in two groups of nonhuman primates. Myocardial tissues taken at the time of autopsy from 50 primates at an experimental center were compared with...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:The existence of facultative stem cells in the liver has been advocated based on observations from models of carcinogenesis in rat liver. Observations of human liver material from cases of fulminant hepatitis have shown the presence of ductular hepatocytes expressing markers of both hepatocytes and bile duct cells. We...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：:In this time of significant change in pathology and medicine, pathology's future is uncertain. Many issues, including the emergence of molecular medicine, the demand for personalized medicine, confusing payment schemes, increasing governmental regulation, medical education challenges, administrative relationships, and...
journal_title：Archives of pathology & laboratory medicine
pub_type： 社论
doi：10.5858/arpa.2011-0563-ED
更新日期：2012-02-01 00:00:00
abstract：CONTEXT:Signet-ring cell carcinoma (SRCC) is a poorly differentiated mucin-producing adenocarcinoma that may arise from many different organs, but all SRCCs share identical morphology. It is not possible to differentiate sites of origin for metastatic SRCC based on morphology alone. Mucins are high-molecular-weight gly...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2006)130[799:MPISCC]2.0.CO;2
更新日期：2006-06-01 00:00:00
abstract：:We report a case of primary cerebral lymphoma metastasizing to the kidney with exclusive involvement of the glomeruli. We discuss the possible explanations of this unusual phenomenon, and present some of the unusual clinical features of the case. Nephropathologists interpreting glomerular hypercellularity should alway...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：CONTEXT.—:Follicular lymphoma is a common small B-cell lymphoma, likely to be encountered by any practicing pathologist, regardless of specialty. Although the features of typical follicular lymphoma are well known and in most instances easily identifiable, there are lesser-appreciated morphologic appearances that can r...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2018-0217-RA
更新日期：2018-11-01 00:00:00
abstract：:We describe the case of a 31-week fetus who died in utero with an invasive retroperitoneal kaposiform hemangioendothelioma. This rare vascular neoplasm usually presents as a localized violaceous skin lesion in infants and behaves in a benign fashion; however, kaposiform hemangioendothelioma may present as an invasive ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2004)128<678:KHAWNF>2.0.CO;2
更新日期：2004-06-01 00:00:00
abstract：:A case of primary fibrosarcoma of the urinary bladder showing extensive chondroid differentiation was studied by light microscopy and immunofluorescent microscopy using tissue-specific antibodies against intermediate filaments. The tumor cells were uniformly and positively labeled with vimentin antibodies and were neg...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:Apoptosis and necrosis are two fundamental types of cell death. Current knowledge indicates that the key mechanism of apoptosis is endonuclease activation leading to internucleosomal double-stranded chromatin (DNA) breaks, whereas the key mechanism of necrosis is cell membrane damage. The initial alterations of cellul...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：
更新日期：1993-12-01 00:00:00
abstract：CONTEXT:Basal-like breast carcinoma (BLBC) is a distinct molecular subtype of breast carcinoma identified through gene expression profiling studies. OBJECTIVE:To provide the clinical background, the histologic profile, and the immunohistochemical profile of these tumors and discuss the current knowledge of their molec...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2012-0439-RA
更新日期：2014-01-01 00:00:00
abstract：:A 50-year-old man presented with a lung mass in the left upper lobe, which was shown by electron microscopy to be a thymoma. There was no evidence of a mediastinal mass. The lymphocytes of the tumor reacted with monoclonal antibody T101, a pan T-cell marker, and with OKT8. B1 and B2 surface antigens characteristic of ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:A 47-year-old man presented to the otolaryngologist with a 7-year history of a mass of the cheek. Fine-needle aspiration revealed foci of spindled cells admixed with abundant fat and myxoid material. A diagnosis of spindle cell lipoma was rendered on the resected specimen. The cytologic findings of spindle cell lipoma...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(2001)125<0820:SCLOTP>2.0.CO;2
更新日期：2001-06-01 00:00:00
abstract：OBJECTIVE:To evaluate choroid plexus neoplasms for p53 expression. CASE MATERIAL:We studied 10 choroid plexus tumors (four papillomas and six carcinomas) by immunohistochemistry using the DO7 anti-p53 monoclonal antibody. RESULTS:Three of four choroid plexus papillomas demonstrated no staining. Scattered nuclear and ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:A case of nongestational, extragonadal choriocarcinoma in a middle-aged man is described. Autopsy examination confirmed that the tumor originated in the lung. The trophoblastic origin of the tumor was documented by immunoperoxidase staining for human chorionic gonadotropin. ...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Pulmonary adenocarcinoma is one of the most common types of lung cancer. Traditionally, adenocarcinomas have been divided based on their degree of resemblance to their parent tissues into 3 histopathologic types: well, moderately, and poorly differentiated. In the majority of cases, this schema is sufficient to catego...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.1043/1543-2165(2006)130[958:PATESO]2.0.CO;2
更新日期：2006-07-01 00:00:00
abstract：:Aspiration biopsy cytology (ABC) specimens were obtained from 540 patients by transrectal biopsy because of suspicious lesions or metastases from occult carcinoma. Although poorly differentiated prostatic carcinoma had previously presented few diagnostic problems by ABC, cells from well-differentiated adenocarcinoma h...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
abstract：CONTEXT:Identification of patients is one of the first steps in ensuring the accuracy of laboratory results. In the United States, hospitalized patients wear wristbands to aid in their identification, but wristbands errors are frequently found. OBJECTIVE:To investigate if continuous monitoring of wristband errors by p...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(2002)126<0809:CWMOYD>2.0.CO;2
更新日期：2002-07-01 00:00:00
abstract：CONTEXT:Chordoma is a rare, notochordal tumor with a characteristic histomorphology and immunohistochemical profile. At times, it presents a diagnostic challenge, especially in small biopsies. Brachyury, a nuclear transcription factor, is a recently described immunohistochemical marker for diagnosing chordomas. OBJECT...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/2009-0476-OA.1
更新日期：2010-08-01 00:00:00
abstract：:Study of 115 kidneys from 60 patients with chronic renal failure maintained by dialysis for two months to five years revealed an unexpected number and variety of epithelial proliferative processes, several types of which are hitherto unreported. Proliferative activity was defined either by the presence of epithelial s...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:A family with a syndrome consisting of granulomatous arthritis, uveitis, and rash was first described in 1985. Another family with the same syndrome was described subsequently in 1990. The condition, in both families, is clinically different from sarcoidosis. To verify whether the granulomas in such patients also coul...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:In this article, we review prostatic lymphomas and discuss the differential diagnosis of high-grade malignant neoplasms of the prostate. We illustrate this with a case of a 46-year-old man seen with lower urinary tract obstruction who had diffuse involvement by a high-grade malignancy on prostate biopsy. Morphologic e...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2014-0276-CC
更新日期：2014-10-01 00:00:00
abstract：:An adenoma of type 2 pneumocytes in the periphery of the left lung was an incidental finding at the autopsy of a 28-year-old woman. Light microscopy revealed a predominantly papillary growth with oncocytic features. Ultrastructurally, multilaminated bodies and many mitochondria were found in the tumor cells and tubula...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:Quantitation of T and B lymphocytes in infants and children is an important test in the diagnosis of a suspected immuno-deficiency. Previous studies indicated that the absolute and relative numbers of lymphocyte subpopulations vary with age, but these data in the pediatric age group are incomplete and often contradict...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：
更新日期：1983-10-01 00:00:00
abstract：:As recipients of tissue and medical specimens, pathologists and other medical specialists regard themselves as stewards of patient tissues and consider it their duty to protect the best interests of both the individual patient and the public. The stewardship of slides, blocks, and other materials includes providing, u...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(1999)123<0296:RPFUOH>2.0.CO;2
更新日期：1999-04-01 00:00:00
abstract：:This article offers a historical perspective on the discovery of 3 types of serrated colorectal polyps recognized in the past 60 years. The first to be discovered was the hyperplastic polyp, which is still the most commonly encountered serrated polyp. In the past 20 years, the carcinoma-associated sessile serrated ade...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2016-0278-RA
更新日期：2016-10-01 00:00:00
abstract：:Values for total lactate dehydrogenase (LDH; EC 1.1.1.27) activity and LDH isoenzymes 1 and 2 were determined in 80 patients with acute myocardial infarction (AMI) and in 40 without AMI every 24 hours up to 15 days after admission, when total serum LDH level returned to normal. The sensitivity, specificity, and effici...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
