Abstract:
:The human NPY Y(1) receptor undergoes fast agonist-induced internalization via clathrin-coated pits then recycles back to the cell membrane. In an attempt to identify the molecular determinants involved in this process, we studied several C-terminal truncation mutants tagged with EFGP. In the absence of agonist, Y(1) receptors lacking the last 32 C-terminal amino acids (Y(1)Δ32) are constitutively internalized, unlike full-length Y(1) receptors. At steady state, internalized Y(1)Δ32 receptors co-localize with transferrin, a marker of early and recycling endosomes. Inhibition of constitutive internalization of Y(1)Δ32 receptors by hypertonic sucrose or by co-expression of Rab5aS34N, a dominant negative form of the small GTPase Rab5a or depletion of all three isoforms of Rab5 indicates the involvement of clathrin-coated pits. In contrast, a truncated receptor lacking the last 42 C-terminal amino acids (Y(1)Δ42) does not constitutively internalize, consistent with the possibility that there is a molecular determinant responsible for constitutive internalization located in the last 10 amino acids of Y(1)Δ32 receptors. We show that the agonist-independent internalization of Y(1)Δ32 receptors involves a tyrosine-based motif YXXΦ. The potential role of this motif in the behaviour of full-length Y(1) receptors has also been explored. Our results indicate that a C-terminal tyrosine-based motif is critical for the constitutive internalization of truncated Y(1)Δ32 receptors. We suggest that this motif is masked in full-length Y(1) receptors which do not constitutively internalize in the absence of agonist.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Lecat S,Ouédraogo M,Cherrier T,Noulet F,Rondé P,Glasser N,Galzi JL,Mely Y,Takeda K,Bucher Bdoi
10.1016/j.cellsig.2010.09.007subject
Has Abstractpub_date
2011-01-01 00:00:00pages
228-38issue
1eissn
0898-6568issn
1873-3913pii
S0898-6568(10)00263-9journal_volume
23pub_type
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journal_title:Cellular signalling
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pub_type: 杂志文章,评审
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2012.11.020
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journal_title:Cellular signalling
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journal_title:Cellular signalling
pub_type: 杂志文章
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journal_title:Cellular signalling
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journal_title:Cellular signalling
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pub_type: 杂志文章,评审
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更新日期:2006-06-01 00:00:00
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journal_title:Cellular signalling
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