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Purification of catalytically active caspase-12 and its biochemical characterization.

Abstract：

:Caspase-12, mainly detected in endoplasmic reticulum (ER), has been suggested to play a role in ER-mediated apoptosis and inflammatory caspase activation pathway. Cleavage of the prodomain by caspase-3/-7 at the carboxyl terminus of Asp94 or m-calpain at the carboxyl terminus of Lys158 was reported to be a part of caspase-12-involved apoptosis. We biochemically characterized the prodomain-free forms of caspase-12 and the equivalent enzymes; Deltapro1(G95-D419), rev-Deltapro1[(T319-N419)-(G95-D318), a reverse form of Deltapro1] and rev-Deltapro2[(T319-N419)-(T159-D318)]. The three variants showed comparable activities which were dependent on salt concentration and pH. Auto-proteolytic cleavage was observed at two sites (carboxyl termini of Asp318 and Asp320) in Deltapro1. Constitutively active forms of caspase-12 (rev-Deltapro1 and rev-Deltapro2) could induce cell death in cells transfected with the corresponding expression vectors, but no cleavage of caspase-3, DFF45 or Bid was observed, indicating caspase-12 may mediate a distinct apoptotic pathway rather than caspase-8 or -9-mediated cell death.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Lee HJ,Lee SH,Park SH,Sharoar MG,Shin SY,Lee JS,Cho B,Park IS

doi

10.1016/j.abb.2010.07.013

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

68-73

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(10)00292-4

journal_volume

502

pub_type

杂志文章

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