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Comparison of wild type neuronal nitric oxide synthase and its Tyr588Phe mutant towards various L-arginine analogues.

Abstract：

:Crystal structures of nitric oxide synthases (NOS) isoforms have shown the presence of a strongly conserved heme active-site residue, Tyr588 (numbering for rat neuronal NOS, nNOS). Preliminary biochemical studies have highlighted its importance in the binding and oxidation to NO of natural substrates L-Arg and N(omega)-hydroxy-L-arginine (NOHA) and suggested its involvement in mechanism. We have used UV-visible and EPR spectroscopy to investigate the effects of the Tyr588 to Phe mutation on the heme-distal environment, on the binding of a large series of guanidines and N-hydroxyguanidines that differ from L-Arg and NOHA by the nature of their alkyl- or aryl-side chain, and on the abilities of wild type (WT) and mutant to oxidize these analogues with formation of NO. Our EPR experiments show that the heme environment of the Tyr588Phe mutant differs from that of WT nNOS. However, the addition of L-Arg to this mutant results in EPR spectra similar to that of WT nNOS. Tyr588Phe mutant binds L-Arg and NOHA with much weaker affinities than WT nNOS but both proteins bind non alpha-amino acid guanidines and N-hydroxyguanidines with close affinities. WT nNOS and mutant do not form NO from the tested guanidines but oxidize several N-hydroxyguanidines with formation of NO in almost identical rates. Our results show that the Tyr588Phe mutation induces structural modifications of the H-bonds network in the heme-distal site that alter the reactivity of the heme. They support recent spectroscopic and mechanistic studies that involve two distinct heme-based active species in the two steps of NOS mechanism.

journal_name

J Inorg Biochem

journal_title

Journal of inorganic biochemistry

authors

Giroud C,Moreau M,Sagami I,Shimizu T,Frapart Y,Mansuy D,Boucher JL

doi

10.1016/j.jinorgbio.2010.06.001

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

1043-50

issue

10

eissn

0162-0134

issn

1873-3344

pii

S0162-0134(10)00142-X

journal_volume

104

pub_type

杂志文章

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