Crystallographically observed folded topology of an unsubstituted γ-aminobutyric acid incorporated in a model peptide: importance of a C--H···O interaction.

abstract：:Side-chain lactam bridges linking amino acid residues that are spaced several residues apart in the linear sequence offer a convenient and flexible method for introducing conformational constraints into a peptide structure. The availability of a variety of selectively cleavable protecting groups for amines and carboxy...

journal_title：Biopolymers

authors： Taylor JW

更新日期：2002-01-01 00:00:00

abstract：:The solid state conformational analysis of [Tyr4] cyclolinopeptide A has been carried out by x-ray diffraction studies. The crystal structure of the monoclinic form, grown from a dioxane-water mixture [alpha = 9.849 (5) A, b = 20.752 (4) A, c = 16.728 (5) A, beta = 98.83 (3) degrees, space group P21, Z = 2], shows the...

journal_title：Biopolymers

authors： Saviano M,Rossi F,Filizola M,Isernia C,Di Blasio B,Benedetti E,Pedone C,Siemion IZ,Pedyczak A

更新日期：1995-10-01 00:00:00

abstract：:The spatial variation in the composition of nonexpanded biopolymer blends prepared by extrusion of mixtures of gelatin with either native or pregelatinized waxy maize starch was studied using a 30-microm aperture FTIR microspectroscopy technique. The ratio of the areas of the "saccharide" bands (953-1180 cm(-1)) and t...

journal_title：Biopolymers

authors： Mousia Z,Farhat IA,Pearson M,Chesters MA,Mitchell JR

更新日期：2001-01-01 00:00:00

abstract：:We review the neighbor exclusion model and some of its applications to analyze the binding data of DNA-ligand complexes. We revisit the closed form of the model developed by McGhee and von Hippel in 1974, showing that this classic model can be used to help studying the behavior of DNA contour and persistence lengths w...

journal_title：Biopolymers

authors： Rocha MS

更新日期：2010-01-01 00:00:00

abstract：:An improved model for calculating the CD of polypeptides has been developed. Excited state wavefunctions were derived from CNDO/S (complete neglect of differential overlap, spectroscopic) calculations on N-methylacetamide. Four discrete peptide-localized transitions were employed: pi 0 pi* (NV1), pi* + pi* (NV2), n pi...

journal_title：Biopolymers

authors： Manning MC,Woody RW

更新日期：1991-04-01 00:00:00

abstract：:The presence of non-native kinetic traps in the free energy landscape of a protein may significantly lengthen the overall folding time so that the folding process becomes unreliable. We use a computational model alpha-helical hairpin peptide to calculate structural free energy landscapes and relate them to the kinetic...

journal_title：Biopolymers

authors： Chapagain PP,Gerstman BS

更新日期：2006-02-15 00:00:00

abstract：:Cyclic pentapeptides are excellent models for reverse turns and have been used extensively in our laboratory to explore the influence of different amino acid sequences on turn preference. This paper is divided into two parts: In the first, we review our previous studies of cyclic pentapeptides. We summarize work that ...

journal_title：Biopolymers

authors： Stradley SJ,Rizo J,Bruch MD,Stroup AN,Gierasch LM

更新日期：1990-01-01 00:00:00

abstract：:In this paper, we explore the possibility of using experimental observations in the Monte Carlo chain growth method that we have previously developed. In this method, the macromolecule (peptide, protein, nuclei acid, etc.) is grown atom-by-atom (or residue-by-residue, etc.) and partial chains are replicated according ...

journal_title：Biopolymers

authors： Bascle J,Garel T,Orland H,Velikson B

更新日期：1993-12-01 00:00:00

abstract：:Cyclotides are multifunctional plant cyclic peptides containing 28-37 amino acid residues and a pattern of three disulfide bridges, forming a motif known as the cyclic cystine knot. Due to their high biotechnological potential, the sequencing and characterization of cyclotide genes are crucial not only for cloning and...

journal_title：Biopolymers

authors： Cunha NB,Barbosa AE,de Almeida RG,Porto WF,Maximiano MR,Álvares LC,Munhoz CB,Eugênio CU,Viana AA,Franco OL,Dias SC

更新日期：2016-11-01 00:00:00

abstract：:The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...

journal_title：Biopolymers

authors： Subirós-Funosas R,El-Faham A,Albericio F

更新日期：2012-01-01 00:00:00

abstract：:Using a variety of spectroscopic techniques, a number of molecular functionalities have been studied in relation to the adsorption process of proteins to air-water interfaces. While ellipsometry and drop tensiometry are used to derive information on adsorbed amount and exerted surface pressure, external reflection cir...

journal_title：Biopolymers

authors： de Jongh HH,Kosters HA,Kudryashova E,Meinders MB,Trofimova D,Wierenga PA

更新日期：2004-05-01 00:00:00

abstract：:Spiders can produce up to seven different types of silks or glues with different mechanical properties. Of these, flagelliform (Flag) silk is the most elastic, and aciniform (AcSp1) silk is the toughest. To produce a chimeric spider silk (spidroin) FlagR -AcSp1R , we fused one repetitive module of flagelliform silk fr...

journal_title：Biopolymers

authors： Tian LY,Meng Q,Lin Y

更新日期：2020-12-01 00:00:00

abstract：:Antagonism of the calcitonin gene-related peptide (CGRP) receptor may be a useful approach for migraine treatment. Selective PEGylated peptide antagonists to the CGRP receptor are described, derived from CGRP(8-37) with polymer derivatization at an engineered lysine-25 residue. Potent PEGylated peptides with improved ...

journal_title：Biopolymers

authors： Miranda LP,Shi L,Holder JR,Wright M,Gegg CV,Johnson E,Wild K,Stenkilsson M,Doellgast G,Manning BH,Salyers K

更新日期：2013-07-01 00:00:00

abstract：:Molecular dynamics simulation, with backbone constraints for 20 ps of equilibration and simulation, of a repeating polypeptide segment, Gln-Pro-His-Gln-Pro-Leu-Gln-Pro-His-Gln-Pro-Leu-Gln-Pro-Met-(Gln-Pro-Leu )4, constituting residues 112-138 of bovine amelolgenin, a 19.35 kD hydrophobic protein, are discussed. It is ...

journal_title：Biopolymers

authors： Renugopalakrishnan V,Pattabiraman N,Prabhakaran M,Strawich E,Glimcher MJ

更新日期：1989-01-01 00:00:00

abstract：:Nucleolar targeting peptides (NrTPs) are a family of cell penetrating peptides (CPPs) derived from crotamine, a rattlesnake venom toxin. They were named NrTPs for their remarkable nucleolus-homing properties and have been studied for their potential as drug delivery vehicles. Live cell microscopy experiments were cond...

journal_title：Biopolymers

authors： Rodrigues M,Andreu D,Santos NC

更新日期：2015-03-01 00:00:00

abstract：:Two-dimensional nmr spectra [correlated spectroscopy (COSY), homonuclear Hartmann-Hahn (HOHAHA), nuclear Overhauser effect spectroscopy (NOESY)] have been observed for cross-linked lysozyme, a chemically modified lysozyme derivative with an extra ester cross-link between residues E35 and W108. Eight shifted cross-peak...

journal_title：Biopolymers

authors： Noda Y,Fukuda Y,Segawa S

更新日期：1997-02-01 00:00:00

abstract：:13C and (31)P NMR spectra of a transmembrane peptide, [1-(13)C]Ala(14)-labeled A(6-34), of bacteriorhodopsin incorporated into dimyristoylphosphatidylcholine (DMPC) bilayer were recorded to clarify its dynamics and orientation in the lipid bilayer. This peptide is shown to take an alpha-helical form both in liquid cry...

journal_title：Biopolymers

authors： Kimura S,Naito A,Tuzi S,Saitô H

更新日期：2002-02-01 00:00:00

abstract：:A novel heptapeptide comprising Ile-Gln-Ser-Pro-His-Phe-Phe (IQSPHFF) identified and found to undergo self-assembly into microparticles in solution. To understand the effects of ultraviolet (UV) irradiation on the self-assembly process, IQSPHFF solutions were exposed to the UV light of 365 nm at room temperature. This...

journal_title：Biopolymers

authors： Wei R,Jin CC,Quan J,Nie HL,Zhu LM

更新日期：2014-03-01 00:00:00

abstract：:The study of biologically active peptides is critical to the understanding of physiological pathways, especially those involved in the development of disease. Historically, the measurement of biologically active endogenous peptides has been undertaken by radioimmunoassay, a highly sensitive and robust technique that p...

journal_title：Biopolymers

authors： Xi L,Jin Y,Parker EA,Josh P,Jones A,Wijffels G,Colgrave ML

更新日期：2012-01-01 00:00:00

abstract：:The interhelical interfaces have been examined in seven high-resolution globin chains. The profiles of hydrophobic contact, as measured by the residue solvent-accessible area loss upon folding, have been calculated. The seven globins studied differ in their overall loss of solvent-accessible area upon packing of their...

journal_title：Biopolymers

authors： Weaver DL

更新日期：1992-05-01 00:00:00

abstract：:Elastin-like polypeptide (ELP) fusions have been designed to allow large-scale, nonchromatographic purification of many soluble proteins by using the inverse transition cycling (ITC) method; however, the sensitivity of the aqueous lower critical solubility phase transition temperature (T(t)) of ELPs to the addition of...

journal_title：Biopolymers

authors： Thapa A,Han W,Simons RH,Chilkoti A,Chi EY,López GP

更新日期：2013-01-01 00:00:00

abstract：:Synthetic model membrane-interactive peptides--both of natural and designed sequence--have become convenient and systematic tools for determination of how the membrane-spanning segments within integral membrane proteins confer protein structure and biology. Conformational studies on these peptides demonstrate that the...

journal_title：Biopolymers

authors： Deber CM,Li SC

更新日期：1995-01-01 00:00:00

abstract：:A large number of potent and selective therapeutic agents, useful for the treatment of several diseases, have been isolated from natural sources. For example, the most active thrombin inhibitors are those secreted by the salivary glands of leeches. One peculiar feature of these agents is the lack of any significant in...

journal_title：Biopolymers

authors： Lombardi A,De Simone G,Galdiero S,Staiano N,Nastri F,Pavone V

更新日期：1999-01-01 00:00:00

abstract：:The rheological characteristics of gastric and duodenal mucin solutions, the building blocks of the mucus layer that covers the epithelia of the two organs, were investigated using particle tracking microrheology. We used biochemically well characterized purified porcine mucins (MUC5AC and MUC2) as models for human mu...

journal_title：Biopolymers

authors： Georgiades P,Pudney PD,Thornton DJ,Waigh TA

更新日期：2014-04-01 00:00:00

abstract：:The use of peptide carriers, termed "cell-penetrating peptides (CPPs)" has attracted much attention due to their potential for cellular delivery of hydrophilic molecules with pharmacological interest, overcoming the membrane barrier. These peptides are able to deliver attached cargos in a nontoxic manner, with the upt...

journal_title：Biopolymers

authors： Henriques ST,Castanho MA,Pattenden LK,Aguilar MI

更新日期：2010-01-01 00:00:00

abstract：:Useful methods of protein labeling via functional peptide tags have been developed in the field of proteome and chemical biology. New tag-probe pairs based on leucine zipper peptides for labeling target proteins are described. These consist of an α-helical probe peptide with an environmental-sensitive fluorescent dye ...

journal_title：Biopolymers

authors： Nomura W,Mino T,Narumi T,Ohashi N,Masuda A,Hashimoto C,Tsutsumi H,Tamamura H

更新日期：2010-01-01 00:00:00

abstract：:Alpha-synuclein (alpha-Syn) has been identified as a component of intracellular fibrillar deposits in Parkinson's disease. Though the real pathogenesis is still unknown, many investigations have revealed that conformational alteration and fibril formation of alpha-Syn protein have an important role in causing the dise...

journal_title：Biopolymers

authors： Lin XJ,Zhang F,Xie YY,Bao WJ,He JH,Hu HY

更新日期：2006-10-15 00:00:00

abstract：:The formation of the KRas4B-PDEδ complex activates different signaling pathways required for the development and maintenance of cancer. Previous experimental and theoretical studies have allowed researchers to design an inhibitor of the KRas4B-PDEδ complex, "Deltarasin." This inhibitor binds to the prenyl-binding pock...

journal_title：Biopolymers

authors： Bello M,Correa-Basurto J,Vargas-Mejía MÁ

更新日期：2019-11-01 00:00:00

abstract：:Molecular dynamics (MD) simulations on stem loop 2 of U1 small nuclear RNA and a construct of the U1A protein were carried out to obtain predictions of the structures for the unbound forms in solution and to elucidate dynamical aspects of induced fit upon binding. A crystal structure of the complex between the U1A pro...

journal_title：Biopolymers

authors： Pitici F,Beveridge DL,Baranger AM

更新日期：2002-12-15 00:00:00

abstract：:Cyclic tri-β-peptide having tetrathiafulvalene (TTF) at the side chain was synthesized to prepare a peptide nanotube aligning TTF side chains along the nanotube. The polarized light microscopic observations revealed crystallization of the cyclic peptide by the vapor diffusion method. Fourier-transform infrared and ele...

journal_title：Biopolymers

authors： Uji H,Kim H,Imai T,Mitani S,Sugiyama J,Kimura S

更新日期：2016-05-01 00:00:00