In vivo reflectance confocal microscopy detects pigmentary changes in melasma at a cellular level resolution.

abstract：:Patch-clamp recordings were carried out in the inside-out configuration in human keratinocytes of the cell line HaCaT. Patch pipettes were filled with 150 mM KCl, 1 mM CaCl(2) and 10 mM HEPES. In symmetrical KCl solutions, single channel currents from a large conductance channel (about 170 pS) were measured. Replaceme...

journal_title：Experimental dermatology

authors： Nguyen VH,Markwardt F

更新日期：2002-08-01 00:00:00

abstract：:Sunburn cell (SBC) formation in the epidermis is a characteristic consequence of ultraviolet radiation (UVR) exposure at doses around or above the minimum erythema dose. SBC have been identified morphologically and biologically as keratinocytes undergoing apoptosis. There is evidence that SBC formation is a protective...

journal_title：Experimental dermatology

authors： Murphy G,Young AR,Wulf HC,Kulms D,Schwarz T

更新日期：2001-06-01 00:00:00

abstract：:Keloid is a dermal proliferative disorder characterized by the excessive proliferation and migration of keratinocytes and fibroblasts. Over-activation of the serine/threonine protein kinase, mammalian target of rapamycin (mTOR), plays a pivotal role in the process. Here, we show that both mTOR complex 1 (mTORC1) and m...

journal_title：Experimental dermatology

authors： Chen J,Liu K,Liu Y,Wang X,Zhang Z

更新日期：2019-03-01 00:00:00

abstract：:Infiltration of eosinophils in atopic dermatitis (AD), which contains inflammatory molecules and cytokines, recruits more inflammatory cells and causes further skin damage. Thymic stromal lymphopoietin (TSLP) is an epithelial cytokine that induces the proinflammatory Th2 immune response and plays an important role in ...

journal_title：Experimental dermatology

authors： Noh JY,Shin JU,Park CO,Lee N,Jin S,Kim SH,Kim JH,Min A,Shin MH,Lee KH

更新日期：2016-11-01 00:00:00

abstract：:Activation of peroxisome proliferator-activated receptors (PPARs) has been shown to have an important role in skin barrier function by regulating differentiation and lipid synthesis in keratinocytes. Oat (Avena sativa) has long been used as a soothing agent to relieve skin irritations, and the clinical benefits of top...

journal_title：Experimental dermatology

authors： Chon SH,Tannahill R,Yao X,Southall MD,Pappas A

更新日期：2015-04-01 00:00:00

abstract：:Psoriasis is a chronic skin disease that affects about 1.5% of the Caucasian population and is characterized by typical macroscopic and microscopic skin alterations. Psoriatic lesions are sharply demarcated, red and slightly raised lesions with silver-whitish scales. The microscopic alterations of psoriatic plaques in...

journal_title：Experimental dermatology

authors： Sabat R,Philipp S,Höflich C,Kreutzer S,Wallace E,Asadullah K,Volk HD,Sterry W,Wolk K

更新日期：2007-10-01 00:00:00

abstract：:Acquisition of potent human dermal papilla (DP) cells that can induce hair follicle neogenesis is an overarching concern, and various approaches have been tried. In an attempt to solve the problem, we previously introduced the three-dimensional (3D) culture of human DP cells and observed de novo formation of hair foll...

journal_title：Experimental dermatology

authors： Seo CH,Kwack MH,Kim MK,Kim JC,Sung YK

更新日期：2017-02-01 00:00:00

abstract：:Mast cells (MCs) have been intensely investigated over the past two decades, e.g. the numbers of PubMed-listed reports on MCs have steadily increased and doubled over the past twenty years. Surprisingly, many recent findings that have fundamentally changed our understanding of MC biology and functions have yet to be s...

journal_title：Experimental dermatology

authors： Maurer M,Metz M

更新日期：2005-12-01 00:00:00

abstract：:Mycosis fungoides and its leukaemic counterpart Sézary syndrome are the most frequent cutaneous T-cell lymphomas (CTCL), and there is no cure for these diseases. We evaluated the effect of clinically approved antihistamines on the growth of CTCL cell lines. CTCL cell lines as well as blood lymphocytes from patients wi...

journal_title：Experimental dermatology

authors： Döbbeling U,Waeckerle-Men Y,Zabel F,Graf N,Kündig TM,Johansen P

更新日期：2013-02-01 00:00:00

abstract：:Irradiation with ultraviolet (UV) B light results in the formation of apoptotic keratinocytes called sunburn cells (SC). Although generation of SC appears to be one of the most characteristic features of UV-induced skin damage and has been a well-known phenomenon for a long time, the mechanisms involved are not quite ...

journal_title：Experimental dermatology

authors： Schwarz A,Mahnke K,Luger TA,Schwarz T

更新日期：1997-02-01 00:00:00

abstract：:Current gold standard lower extremity cutaneous wound management is not always effective. Cutaneous wounds generate a "current of injury" which is directly involved in wound healing processes. Application of exogenous electrical stimulation has been hypothesised to imitate the natural electric current that occurs in c...

journal_title：Experimental dermatology

authors： Ashrafi M,Alonso-Rasgado T,Baguneid M,Bayat A

更新日期：2017-02-01 00:00:00

abstract：:Reverse transcriptase qPCR is the most common method to determine and compare mRNA expression levels and relies on normalization using reference genes. The expression levels of the latter, however, are themselves often variable between experimental conditions, thus compromising the results. Using the geNorm algorithm,...

journal_title：Experimental dermatology

authors： Braun A,Sezin T,Bezdek S,Doxastaki I,Zillikens D,Busch HS,Sadik CD

更新日期：2019-08-01 00:00:00

abstract：:Recently, 3% diclofenacnatrium gel (diclofenac) was introduced for the treatment of actinic keratoses. Data on photocarcinogenesis of topical diclofenac are limited, and we wished to investigate whether topical diclofenac can accelerate photocarcinogenesis using simulated solar radiation (SSR). Diclofenac was applied ...

journal_title：Experimental dermatology

authors： Lerche CM,Philipsen PA,Poulsen T,Wulf HC

更新日期：2011-04-01 00:00:00

abstract：:IL-15 has emerged as a potentially relevant target in the IL-17 response in psoriasis. However, its mechanism is poorly characterized in humans. IL-15 and IL-23 are constitutively expressed in the psoriatic lesion. Also, IL-15 is considered a susceptibility-associated gene in psoriasis, as are IL-23R, and HLACW6. Here...

journal_title：Experimental dermatology

authors： de Jesús-Gil C,Ruiz-Romeu E,Ferran M,Sagristà M,Chiriac A,García P,Celada A,Pujol RM,Santamaria-Babí LF

更新日期：2020-05-31 00:00:00

abstract：:In the last decade, significant progress has been made in understanding skin cancer cell death resistance mechanisms, and a number of new treatment strategies have been developed. Systematic approach genomic studies of various cancer types have opened new possibilities for the development of anticancer therapies. Howe...

journal_title：Experimental dermatology

authors： Feoktistova M,Panayotova-Dimitrova D

更新日期：2017-10-01 00:00:00

abstract：:In the 1970s, studies of tissue architecture and cell proliferation were used to formulate a new model of epidermal homeostasis. This asserted that the tissue was maintained by long-lived, slow-cycling, self-renewing stem cells that generate a short-lived population of transit amplifying (TA) cells, which undergo term...

journal_title：Experimental dermatology

authors： Doupé DP,Jones PH

更新日期：2012-04-01 00:00:00

abstract：:Volatile organic compounds emitted by human skin were sampled before and after acute barrier disruption of the volar forearm to investigate the significance of this approach to skin physiology research. A small wearable housing integrating a solid-phase micro-extraction fibre permitting rapid enclosed headspace sampli...

journal_title：Experimental dermatology

authors： Duffy E,Jacobs MR,Kirby B,Morrin A

更新日期：2017-10-01 00:00:00

abstract：:We have yet to create a human scar model that demonstrates the complex nature of hypertrophic scar and keloid formation as well as ways to prevent them despite emerging advances in our understanding of the immune system, the inflammatory response, and proteomic and genomic changes after injury. Despite more complex in...

journal_title：Experimental dermatology

authors： Williams FN,Herndon DN,Branski LK

更新日期：2014-11-01 00:00:00

abstract：:SHARPIN is an important component of the linear ubiquitin chain assembly complex (LUBAC). Loss of function of SHARPIN results in eosinophilic inflammation in multiple organs including skin with Th2 -dominant cytokines and dysregulated development of lymphoid tissues in mice. The clinicopathological features are simila...

journal_title：Experimental dermatology

authors： Tang L,Wang J,Zhu J,Liang Y

更新日期：2018-12-01 00:00:00

abstract：:Murine model systems are critically required tools for the investigation of unknown mechanisms of melanoma development and metastasis and for developing more efficient therapies. The Tg(Grm1)EPv melanoma mouse model is characterized by spontaneous development of pigmented cutaneous melanomas at hairless skin regions, ...

journal_title：Experimental dermatology

authors： Schiffner S,Chen S,Becker JC,Bosserhoff AK

更新日期：2012-10-01 00:00:00

abstract：:Despite the increasing use of gene transfer strategies in the study of cellular and molecular biology, melanoma cells have remained difficult to transfect in a safe, efficient, and reproducible manner. In the present study, we report the successful use of nucleofector technology to transfect human melanoma cell lines....

journal_title：Experimental dermatology

authors： Han SY,Gai W,Yancovitz M,Osman I,Di Como CJ,Polsky D

更新日期：2008-05-01 00:00:00

abstract：:Corticotropin releasing factor, adrenocorticotropic hormone (ACTH) and alpha-melanocyte stimulating hormone either inhibit or enhance in a dose-dependent fashion an interleukin-4 (IL-4) driven human IgE synthesis in vitro. Here, we show that culture conditions strongly influence the earlier observed dose- and donor-de...

journal_title：Experimental dermatology

authors： Aebischer I,Stämpfli MR,Miescher S,Horn M,Zürcher AW,Stadler BM

更新日期：1996-02-01 00:00:00

abstract：:Interferon-γ (IFNγ)-induced collapse of hair follicle (HF) immune privilege (IP) is a key element in the pathogenesis of alopecia areata. In this pilot study, we investigated whether the immunosuppressive neuropeptide, calcitonin gene-related peptide (CGRP), can protect from and/or restore IFNγ-induced HF-IP collapse....

journal_title：Experimental dermatology

authors： Kinori M,Bertolini M,Funk W,Samuelov L,Meyer KC,Emelianov VU,Hasse S,Paus R

更新日期：2012-03-01 00:00:00

abstract：:We have previously reported that lymph node cells generated chemotactic factors for Langerhans cells (LCs) in the induction phase of contact dermatitis. In order to clarify the chemotactic factors involved in migration to the regional lymph nodes, we investigated the migratory activity of murine LCs toward several cyt...

journal_title：Experimental dermatology

authors： Yamazaki S,Yokozeki H,Satoh T,Katayama I,Nishioka K

更新日期：1998-02-01 00:00:00

abstract：:T cells play a major role in inflammatory skin disorders such as psoriasis vulgaris and atopic dermatitis. They are both active on the level of cell-to-cell interaction and by the secretion of pro-inflammatory mediators. CD26 is a lymphocyte membrane-associated dipeptidyl peptidase IV (DPP IV), which is able to inacti...

journal_title：Experimental dermatology

authors： Bock O,Kreiselmeyer I,Mrowietz U

更新日期：2001-12-01 00:00:00

abstract：:Dermal mast cells protect the skin from inflammatory effects of ultraviolet (UV) radiation and are required for UV-induced immune suppression. We sought to determine a potential mechanistic role of mast cells in reducing the sensitivity to UV radiation (i.e. phototolerance induction) through photohardening. We adminis...

journal_title：Experimental dermatology

authors： Schweintzger NA,Bambach I,Reginato E,Mayer G,Limón-Flores AY,Ullrich SE,Byrne SN,Wolf P

更新日期：2015-07-01 00:00:00

abstract：:A two-stage genomewide association (GWA) analysis was conducted to investigate the genetic factors influencing ultraviolet (UV)-induced skin pigmentation in Korean females after UV exposure. Previously, a GWA study evaluating ~500 000 single nucleotide polymorphisms (SNPs) in 99 Korean females identified eight SNPs th...

journal_title：Experimental dermatology

authors： Kwak TJ,Chang YH,Shin YA,Shin JM,Kim JH,Lim SK,Lee SH,Lee MG,Yoon TJ,Kim CD,Lee JH,Koh JS,Seo YK,Chang MY,Lee Y

更新日期：2015-12-01 00:00:00

abstract：:Inter-α-trypsin inhibitors are protease inhibitors that are thought to be important regulators in various acute-phase processes. They are composed of one light chain (bikunin) and different heavy chains (ITIHs). The only function known so far of ITIHs is the covalent linkage to hyaluronan (HA). As there is virtually n...

journal_title：Experimental dermatology

authors： Huth S,Heise R,Vetter-Kauczok CS,Skazik C,Marquardt Y,Czaja K,Knüchel R,Merk HF,Dahl E,Baron JM

更新日期：2015-09-01 00:00:00

abstract：:Psoriasis is an inflammatory skin disease often associated with obesity. The anti-inflammatory adipokine vaspin, a suggested serine proteinase inhibitor of the serpin family, is discussed as a new link between inflammation and obesity. Here, we demonstrate that - different from healthy controls - vaspin serum levels i...

journal_title：Experimental dermatology

authors： Saalbach A,Vester K,Rall K,Tremel J,Anderegg U,Beck-Sickinger AG,Blüher M,Simon JC

更新日期：2012-04-01 00:00:00

abstract：:Systemic sclerosis (SSc) is a complex autoimmune connective tissue disease combining inflammatory, vasculopathic and fibrotic manifestations. Skin features, which give their name to the disease and are considered as diagnostic as well as prognostic markers, have not been thoroughly investigated in terms of therapeutic...

journal_title：Experimental dermatology

authors： Henrot P,Truchetet ME,Fisher G,Taïeb A,Cario M

更新日期：2019-01-01 00:00:00