High death rate in mice treated topically with diclofenac.


:Recently, 3% diclofenacnatrium gel (diclofenac) was introduced for the treatment of actinic keratoses. Data on photocarcinogenesis of topical diclofenac are limited, and we wished to investigate whether topical diclofenac can accelerate photocarcinogenesis using simulated solar radiation (SSR). Diclofenac was applied topically on the backs of hairless, female, C3.Cg/TifBomTac immunocompetent mice three times weekly followed by ultraviolet radiation (2, 3, or 4 Standard Erythema Dose) until death. There was a significant difference in survival between diclofenac-treated groups and control groups (P<0.0001). Physical examination of the diclofenac-treated mice showed peptic ulcers, oesophageal ulcers and gastrointestinal bleeding. To be sure that these side effects were not caused by topical absorption without oral ingestion, one group of mice was wearing Elizabethan collars and was single housed. Nevertheless, these mice also had gastrointestinal side effects. We terminated the experiment after 151 days when only a few mice remained in the diclofenac-treated groups and most had symptoms of discomfort and weight loss. No tumors developed as a result of the early termination.


Exp Dermatol


Experimental dermatology


Lerche CM,Philipsen PA,Poulsen T,Wulf HC




Has Abstract


2011-04-01 00:00:00












  • A novel mutation in exon 8 of C1 inhibitor (C1INH) gene leads to abolish its physiological stop codon in a large Chinese family with hereditary angioedema type I.

    abstract::C1 inhibitor (C1INH) plays an important role in the classical pathway of the complement system. Mutations in C1INH gene cause quantitative or qualitative deficiencies in C1INH, which can lead to hereditary angioedema (HAE) type I or II. Here, we identified a novel frame-shift mutation c.1391-1445del55 (p.v464fsx556) i...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Qu L,Wei B,Liu M,Zhang L,Xiao T,Chen HD,Zhou L,Mi QS,He C

    更新日期:2012-10-01 00:00:00

  • Cleavage of desmoglein 3 can explain its depletion from keratinocytes in pemphigus vulgaris.

    abstract::We have previously demonstrated that serum of patients with pemphigus vulgaris induces reduction of desmoglein 3 (Dsg3) half-life in keratinocytes (FEBS Lett 2006: 580: 3276). This phenomenon seems to occur as a consequence of the progressive depletion of Dsg3 from desmosomes. Here we reported that reduction of full-l...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Cirillo N,Campisi G,Gombos F,Perillo L,Femiano F,Lanza A

    更新日期:2008-10-01 00:00:00

  • Germline variation of the melanocortin-1 receptor does not explain shared risk for melanoma and thyroid cancer.

    abstract:BACKGROUND:Recently, germline variants of the melanocortin-1 receptor (MC1R) have been shown to be associated with an increased risk for BRAF mutant but not BRAF wild-type cutaneous melanoma. Similar to melanoma, BRAF mutations are also commonly found in papillary thyroid carcinomas. Furthermore, patients with melanoma...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Bauer J,Weng J,Kebebew E,Soares P,Trovisco V,Bastian BC

    更新日期:2009-06-01 00:00:00

  • Enzymes involved in the conversion of arachidonic acid to eicosanoids in the skin of atopic dogs.

    abstract::Canine atopic dermatitis (AD), a chronic inflammatory skin disease, shares characteristics with its human counterpart. To get insight into the role of enzymes involved in production of prostaglandin E(2) (PGE2) and leukotriene B(4) (LTB(4)), potent inflammatory mediators originating from membrane-derived arachidonic a...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Schlotter YM,Riemers FM,Rutten VP,Knol EF,Willemse T

    更新日期:2010-08-01 00:00:00

  • Promoter region polymorphism in the human TNF-alpha gene is not associated with lichen sclerosus.

    abstract::The pathogenesis of lichen sclerosus remains unknown. However, it has been frequently associated clinically with autoimmunity. The MHC haplotype A1, B8, DR3 is associated with many autoimmune conditions and has also been associated with the uncommon allele of the tumour necrosis factor (TNF-alpha) promoter polymorphis...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Clay FE,Cork MJ,Wilson AG,Crane AM,Lewis F,Harrington CI,Duff GW

    更新日期:1996-08-01 00:00:00

  • Bifidobacterium longum lysate, a new ingredient for reactive skin.

    abstract::Reactive skin is characterized by marked sensitivity to physical (heat, cold, wind) or chemical (topically applied products) stimuli and by the impairment of the skin barrier's ability to repair itself. Several lines of evidence suggest that beyond their capacity to positively influence the composition of intestinal m...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,随机对照试验


    authors: Guéniche A,Bastien P,Ovigne JM,Kermici M,Courchay G,Chevalier V,Breton L,Castiel-Higounenc I

    更新日期:2010-08-01 00:00:00

  • A novel mutation of the glomulin gene in an Italian family with autosomal dominant cutaneous glomuvenous malformations.

    abstract::Glomuvenous malformations (GVM) are hamartomas characterized histologically by glomus cells, which should be distinguished from glomus tumors. Familial GVM are rare, often present as multiple lesions, and exhibit familial aggregation, with autosomal dominant transmission. GVM are caused by mutations of the glomulin (G...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Borroni RG,Narula N,Diegoli M,Grasso M,Concardi M,Rosso R,Cerica A,Brazzelli V,Arbustini E

    更新日期:2011-12-01 00:00:00

  • Naringenin as an opener of mitochondrial potassium channels in dermal fibroblasts.

    abstract::Flavonoids belong to a large group of polyphenolic compounds that are widely present in plants. Certain flavonoids, including naringenin, have cytoprotective properties. Although the antioxidant effect has long been thought to be a crucial factor accounting for the cellular effects of flavonoids, mitochondrial channel...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Kampa RP,Kicinska A,Jarmuszkiewicz W,Pasikowska-Piwko M,Dolegowska B,Debowska R,Szewczyk A,Bednarczyk P

    更新日期:2019-05-01 00:00:00

  • Bioactive tetrapeptide GEKG boosts extracellular matrix formation: in vitro and in vivo molecular and clinical proof.

    abstract::The 'matrikine' concept claims that processing of the precursors for collagen results in the formation of peptides such as KTTKS which in turn augments extracellular matrix (ECM) production. In the present study, we show the development of an anti-ageing active from an in silico approach by molecular design resulting ...

    journal_title:Experimental dermatology

    pub_type: 信件,随机对照试验


    authors: Farwick M,Grether-Beck S,Marini A,Maczkiewitz U,Lange J,Köhler T,Lersch P,Falla T,Felsner I,Brenden H,Jaenicke T,Franke S,Krutmann J

    更新日期:2011-07-01 00:00:00

  • Leptin, adiponectin, visfatin and retinol-binding protein-4 - mediators of comorbidities in patients with psoriasis?

    abstract::White adipose tissue is known to be involved in numerous physiological processes such as insulin-mediated functions, lipid and glucose metabolism, vascular changes and coagulation. These processes are mainly mediated by adipokines that are secreted either from adipocytes or cells of the stromal-vascular fraction of ad...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Gerdes S,Osadtschy S,Rostami-Yazdi M,Buhles N,Weichenthal M,Mrowietz U

    更新日期:2012-01-01 00:00:00

  • How realistic is cutaneous gene therapy?

    abstract::Recent progress with innovative, experimental gene therapy approaches in animals, and recent improvements in our understanding and manipulation of stem cells, gene expression and gene delivery systems, have raised plenty of hopes in essentially all branches of clinical medicine that hitherto untreatable or poorly mana...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Hengge UR,Taichman LB,Kaur P,Rogers G,Jensen TG,Goldsmith LA,Rees JL,Christiano AM

    更新日期:1999-10-01 00:00:00

  • What is new in the psychology of chronic itch?

    abstract::Itch is often regarded as unpleasant or bothersome and is accompanied by symptoms of distress and impairments in daily life. The biopsychosocial model of chronic itch describes how psychological factors can contribute to the improvement or exacerbation of chronic itch and related scratching behaviour. Recent research ...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Evers AWM,Peerdeman KJ,van Laarhoven AIM

    更新日期:2019-12-01 00:00:00

  • Serum levels of high mobility group box 1 correlate with disease severity in recessive dystrophic epidermolysis bullosa.

    abstract::In the inherited blistering skin disease, recessive dystrophic epidermolysis bullosa (RDEB), there is clinical heterogeneity with variable scarring and susceptibility to malignancy. Currently, however, there are few biochemical markers of tissue inflammation or disease progression. We assessed whether the non-histone ...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Petrof G,Abdul-Wahab A,Proudfoot L,Pramanik R,Mellerio JE,McGrath JA

    更新日期:2013-06-01 00:00:00

  • A novel lateral flow immunoassay for the rapid detection of anti-Dsg3 IgG serum autoantibodies in pemphigus vulgaris.

    abstract::Pemphigus vulgaris (PV) is a severe autoimmune blistering disease of the skin and mucous membranes. As autoantibodies play an essential role in the disease pathogenesis, the serological detection of anti-desmoglein 3 IgG represents a central tool in the diagnosis of the disease. In this study, we show the validation o...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Schmidt T,Mauracher S,Bender L,Greene B,Kurzhals J,Eming R,Dostatni R,Hertl M

    更新日期:2018-03-01 00:00:00

  • Basal cell carcinomas display extensive abnormalities in the hemidesmosome anchoring fibril complex.

    abstract::We have employed a panel of antibodies directed against several newly-defined and well-characterized components of the epidermal basement membrane (BM) to investigate the biology of basal cell carcinoma (BCC) by indirect immunofluorescence and to determine whether alterations in BM components may play a significant ro...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Korman NJ,Hrabovsky SL

    更新日期:1993-06-01 00:00:00

  • CAR-T cell therapy in melanoma: A future success story?

    abstract::Chimeric antigen receptor (CAR)-T cells are one of the impressive recent success stories of anti-cancer immunotherapy. Especially in haematological malignancies, this treatment strategy has shown promising results leading to the recent approval of two CAR-T cell constructs targeting CD19 in the United States and the E...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Simon B,Uslu U

    更新日期:2018-12-01 00:00:00

  • Patterning skin by planar cell polarity: the multi-talented hair designer.

    abstract::In mammals, the skin can form complex global and local patterns to meet diverse functional requirements in different parts of the body. To date, the fundamental principles that underlie skin patterning remain poorly understood because of the involvement of multiple interacting processes. Genes involved in the planar c...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Chen J,Chuong CM

    更新日期:2012-02-01 00:00:00

  • The single-chain anti-TNF-α antibody DLX105 induces clinical and biomarker responses upon local administration in patients with chronic plaque-type psoriasis.

    abstract::It is not clear whether TNF-α antagonists used in the treatment of psoriasis need to act systemically, or whether local inhibition of skin-produced TNF-α would be sufficient to silence skin inflammation. To answer this question, we conducted two multicentre, double-blinded, randomized, placebo-controlled clinical tria...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Tsianakas A,Brunner PM,Ghoreschi K,Berger C,Loser K,Röcken M,Stingl G,Luger T,Jung T

    更新日期:2016-06-01 00:00:00

  • hMLH1 and hMSH2 gene mutations are present in radial growth-phase cutaneous malignant melanoma cell lines and can be induced further by ultraviolet-B irradiation.

    abstract::Microsatellite instability and reduced expression of mismatch repair proteins were reported in melanomas. However, little is known about mutational changes of the mismatch repair genes in radial growth-phase melanoma especially following UVB irradiation. To investigate these changes, an in vitro system consisting of r...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Hussein MR,Wood GS

    更新日期:2003-12-01 00:00:00

  • Messenger RNAs for the multifunctional cytokines interleukin-1 alpha, interleukin-1 beta and tumor necrosis factor-alpha are present in adnexal tissues and in dermis of normal human skin.

    abstract::Interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) are 3 cytokines that play a key rôle in cutaneous homeostasis and in the immunopathogenesis of a number of dermatologic diseases. Most studies have focused on their production by keratinocytes and Langerhans ce...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Boehm KD,Yun JK,Strohl KP,Elmets CA

    更新日期:1995-12-01 00:00:00

  • Chemoprevention of photocarcinogenesis by lycopene.

    abstract::This review focuses on the photoprotection conferred by lycopene, one of the most potent anti-oxidants. Lycopene has been recently proposed to play a critical role on anticarcinogenic action at different levels. The photoprotective properties of lycopene remain contradictory. Some studies point out a positive and othe...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,评审


    authors: Ascenso A,Ribeiro H,Marques HC,Oliveira H,Santos C,Simões S

    更新日期:2014-12-01 00:00:00

  • Prolyl and lysyl hydroxylases in collagen synthesis.

    abstract::Collagens are the most abundant proteins in the extracellular matrix. They provide a framework to build organs and tissues and give structural support to make them resistant to mechanical load and forces. Several intra- and extracellular modifications are needed to make functional collagen molecules, intracellular pos...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,评审


    authors: Salo AM,Myllyharju J

    更新日期:2021-01-01 00:00:00

  • E-FABP induces differentiation in normal human keratinocytes and modulates the differentiation process in psoriatic keratinocytes in vitro.

    abstract::Epidermal fatty acid-binding protein (E-FABP) is a lipid carrier, originally discovered in human epidermis. We show that E-FABP is almost exclusively expressed in postmitotic (PM) keratinocytes, corresponding to its localization in the highest suprabasal layers, while it is barely expressed in keratinocyte stem cells ...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Dallaglio K,Marconi A,Truzzi F,Lotti R,Palazzo E,Petrachi T,Saltari A,Coppini M,Pincelli C

    更新日期:2013-04-01 00:00:00

  • SIRT2 as a new player in epigenetic programming of keratinocyte differentiation and a candidate tumor suppressor.

    abstract::Epidermal keratinocytes undergo a continuous process of terminal differentiation, which is accompanied by a dramatic change in the expression and composition of keratins. This complex and carefully orchestrated process is regulated by a large number of signal transduction events and transcriptional factors as well as ...

    journal_title:Experimental dermatology

    pub_type: 评论,杂志文章


    authors: Wang M,Yue Z,Paus R,Ramot Y

    更新日期:2014-09-01 00:00:00

  • Adiponectin expression is decreased in the involved skin and sera of diffuse cutaneous scleroderma patients.

    abstract::In this study, we determined the adiponectin expression in the serum and lesional skin of patients with scleroderma (SSc). Serum adiponectin concentrations were measured in 32 patients with SSc, 10 patients with SLE, 12 patients with dermatomyositis patients and 13 healthy subjects with specific enzyme-linked immunoso...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Arakawa H,Jinnin M,Muchemwa FC,Makino T,Kajihara I,Makino K,Honda N,Sakai K,Fukushima S,Ihn H

    更新日期:2011-09-01 00:00:00

  • Germline PTEN mutations in three families with Cowden syndrome.

    abstract::Cowden syndrome (CS) is an autosomal dominant inherited disorder characterized by hamartomas in a variety of tissues including the skin, thyroid, breast, endometrium, and the brain. Individuals with CS are predisposed to development of malignancy in these organs, especially the breast and the thyroid. We describe 3 un...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Celebi JT,Ping XL,Zhang H,Remington T,Sulica VI,Tsou HC,Peacocke M

    更新日期:2000-04-01 00:00:00

  • Immunopathogenesis of psoriasis.

    abstract::Psoriasis is a chronic skin disease that affects about 1.5% of the Caucasian population and is characterized by typical macroscopic and microscopic skin alterations. Psoriatic lesions are sharply demarcated, red and slightly raised lesions with silver-whitish scales. The microscopic alterations of psoriatic plaques in...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,评审


    authors: Sabat R,Philipp S,Höflich C,Kreutzer S,Wallace E,Asadullah K,Volk HD,Sterry W,Wolk K

    更新日期:2007-10-01 00:00:00

  • Modulation of keratinocyte growth factor (KGF) mRNA expression in human dermal fibroblasts grown in monolayer or within a collagen matrix.

    abstract::In this study, we analysed the modulation of keratinocyte growth factor (KGF) mRNA expression in human dermal fibroblasts cultured either in monolayer or within a collagen matrix (dermal equivalent). In monolayer cultures, KGF expression by quiescent fibroblasts was stimulated by different growth substances such as se...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Le Panse R,Bouchard B,Lebreton C,Coulomb B

    更新日期:1996-04-01 00:00:00

  • Basal cell carcinoma--an in-vivo model of human tumour microcirculation?

    abstract::The role of angiogenesis in tumour growth and metastasis is well established. However, investigations of tumour microcirculation to date have used either biopsy material from human tumours, or animal models in vivo. We have studied the tumour microcirculation in vivo in human skin cancers using video-microscopy to exa...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Bedlow AJ,Stanton AW,Cliff S,Mortimer PS

    更新日期:1999-06-01 00:00:00

  • Characterization of circadian human facial surface lipid composition.

    abstract:BACKGROUND:The circadian rhythm is an endogenous clock that governs a wide range of physiological functions. In the skin, rhythmic changes in skin barrier function have been investigated at the physiological level; however, few studies at the molecular level have been reported. Additionally, there is no study on lipido...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Jia Y,Zhou M,Huang H,Gan Y,Yang M,Ding R

    更新日期:2019-07-01 00:00:00