Abstract:
OBJECTIVES:The present study was conducted to monitor the expression of pancreas and duodenal homeobox gene (PDX-1) for assessing beta-cell function in islets from patients with chronic pancreatitis (CP). METHODS:Islets isolated from the pancreata of 40 surgical patients categorized as control group, patients with mild CP, and patients with advanced CP were assessed for their yield, size, and glucose-stimulated insulin secretion. Expressions of genes coding for PDX-1, insulin, and glucagon were simultaneously monitored by reverse transcription polymerase chain reaction and confirmed by immunohistochemistry. RESULTS:In comparison with the control group (2673 +/- 592 islet equivalents [IEq]/g), islet yield did not differ much in the patients with mild CP (2344 +/- 738 IEq/g) but was significantly reduced (P < 0.0001) in the patients with advanced CP (731 +/- 167 IEq/g). Although the marginal decrease in islet size observed in the patients with mild CP was not significantly different from that observed in the control group, there was a 58% decrease observed in the patients with advanced CP that was also accompanied by a significant reduction in beta-cell mass (P < 0.05). The expression of insulin and PDX-1 genes, but not of glucagon, was significantly reduced in the patients with advanced CP as confirmed by immunohistochemistry. Islets obtained from the patients with advanced CP retained 53% glucose-stimulated insulin secretion function in comparison with those of the control group. CONCLUSION:The results indicate that beta-cell dysfunction during progression of CP correlates with the decrease in PDX-1 gene expression.
journal_name
Pancreasjournal_title
Pancreasauthors
Mitnala S,Pondugala PK,Guduru VR,Rabella P,Thiyyari J,Chivukula S,Boddupalli S,Hardikar AA,Reddy DNdoi
10.1097/MPA.0b013e3181d6bc69subject
Has Abstractpub_date
2010-08-01 00:00:00pages
856-62issue
6eissn
0885-3177issn
1536-4828journal_volume
39pub_type
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