Abstract:
BACKGROUND & AIMS:Histopathology is the gold standard in the diagnosis of liver diseases but may be complicated by sampling error and bleeding. Confocal laser endomicroscopy (CLE) is a novel imaging modality providing in vivo histology. Previous studies using CLE for liver microscopy suffered from limited imaging depth using blue laser light and fluorescein. The aim of the current study was to evaluate a novel near-infrared (NIR) light probe with indocyanine green (ICG) contrast for in vivo confocal imaging of the human liver during mini-laparoscopy. METHODS:The hand-held rigid laparoscopy probe (diameter 6.3mm) used a 780 nm diode laser. Subsurface images at different depths with < 1 microm lateral resolution were generated in real time by gently placing the sterile probe onto the liver in 22 patients under conscious analgo-sedation. Targeted biopsy was performed for histopathological correlation. RESULTS:Maximum imaging depth was >350 microm. Typical aspects of normal liver architecture and liver diseases, such as nuclei, sinusoids, fibrous tissue, fatty inclusions, and bile ducts but not inflammation could be visualized at high resolution. The presence of steatosis and fibrosis was predicted correctly in 81% and 90%, respectively. No liver damage or severe adverse events occurred. CONCLUSIONS:For the first time, ICG-augmented confocal mini-laparoscopy with a novel NIR probe allowed in vivo microscopy of human liver diseases, and with deeper imaging compared to the so far available blue laser systems. Such an increase of imaging depth could potentially also be used for submucosal imaging of the GI tract.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Goetz M,Deris I,Vieth M,Murr E,Hoffman A,Delaney P,Galle PR,Neurath MF,Kiesslich Rdoi
10.1016/j.jhep.2010.01.039subject
Has Abstractpub_date
2010-07-01 00:00:00pages
84-90issue
1eissn
0168-8278issn
1600-0641pii
S0168-8278(10)00193-5journal_volume
53pub_type
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abstract::Hepatitis C infection is common in patients undergoing liver transplantation. Few studies have focused on the prevalence and epidemiology of hepatitis C infection among liver transplant recipients since the implementation of donor screening for antibodies against the hepatitis C virus (anti-HCV). Using reverse transcr...
journal_title:Journal of hepatology
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doi:
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,多中心研究
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pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00