Abstract：
BACKGROUND/AIMS:The x gene of hepatitis B virus encodes a transactivating factor of 154 amino acids, termed HBx, which stimulates transcription of multiple viral and cellular genes. The transactivating function is probably associated with a tumorigenic potential of HBx, since x gene sequences, encoding functional HBx, have been repeatedly found integrated into the genome of liver carcinoma cells. METHODS:To identify the transactivating domain of HBx, we constructed x gene plasmids encoding full length HBx or HBx fragments. We determined their transactivating function after cotransfection of cells, along with a plasmid that contains a reporter gene driven by the SV40 early promoter/enhancer region. RESULTS:Our results demonstrate that a 95-amino acid fragment of HBx, encompassing amino acids 49 to 143, contains all the elements that are required for the transactivating function. Within this fragment a sequence element, encompassing amino acids 107 to 130, which contains a relatively high number of amino acids with charged side chains, appears to be crucial for the stimulation of gene expression. The influence of deletion mutations on x mRNA steady-state levels and HBx stability was examined. In essence, stable RNA and protein were produced if at least codons 1-82 or 70-154 were present in the deletion plasmids. CONCLUSION:This finding strongly suggests that the deletion of functional domains between codons 49 and 143, but not an instability of RNA and/or protein, was critical for the loss of transactivation.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Renner M,Haniel A,Bürgelt E,Hofschneider PH,Koch Wdoi
10.1016/0168-8278(95)80311-4subject
Has Abstractpub_date
1995-07-01 00:00:00pages
53-65issue
1eissn
0168-8278issn
1600-0641pii
0168-8278(95)80311-4journal_volume
23pub_type
杂志文章abstract：:To study experimentally the protective effect of post-exposure prophylaxis against hepatitis B (HB), a special preparation of hepatitis B immune globulin (HBIG) was injected intravenously (i.v.) into three chimpanzees simultaneously with, or at different time intervals after, intravenous injection of a titred inoculum...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(89)90051-2
更新日期：1989-09-01 00:00:00
abstract：BACKGROUND/AIMS:The immunological response of ducks to acute infection with duck hepatitis B virus (DHBV) has not been fully characterised. In this study the relationship between viral dose and the outcome of infection in immune competent 26-day-old ducks was examined. METHODS:Indirect ELISA assays were developed to d...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(96)80210-8
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND & AIMS:Although the growth suppressing Hippo pathway has been implicated in hepatocellular carcinoma (HCC) pathogenesis, it is unknown at which stage of hepatocarcinogenesis its dysregulation occurs. We investigated in rat and human preneoplastic lesions whether overexpression of the transcriptional co-activ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2014.06.033
更新日期：2014-11-01 00:00:00
abstract：BACKGROUND/AIMS:The chemokine receptor CX3CR1 and its specific ligand fractalkine (CX3CL1) are known to modulate inflammatory and fibroproliferative diseases. Here we investigate the role of CX3CR1/fractalkine in HCV-induced liver fibrosis. METHODS:A genotype analysis of CX3CR1 variants was performed in 211 HCV-infect...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2007.09.008
更新日期：2008-02-01 00:00:00
abstract：BACKGROUND & AIMS:Liver transplantation is a recognized treatment for selected patients with hepatocellular carcinoma (HCC), but transplant criteria still need to be refined, especially in the case of more advanced or downstaged tumors. METHODS:The present study investigated alpha-fetoprotein (AFP) as a predictor of o...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.12.040
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND/AIMS:Circulatory abnormalities with activation of vasoconstrictor systems after large-volume paracentesis are generally considered secondary to an increased extravasation of fluid from the intravascular compartment to the extravascular space with subsequent reduction in plasma volume. To test this hypothesis...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80081-5
更新日期：1997-10-01 00:00:00
abstract：BACKGROUND/AIMS:Most hepatocellular carcinomas are still discovered at an advanced stage and are left untreated as large hepatocellular carcinomas are contraindications to liver transplantation and percutaneous ethanol injection and are usually considered as poor indications for liver resection. The aim of this study w...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80319-5
更新日期：1999-12-01 00:00:00
abstract：BACKGROUND/AIMS:The aim of our study was to evaluate the proportion of patients with severe alcoholic cirrhosis who would need orthotopic liver transplantation (OLT) and to determine the optimal delay to evaluate an abstinent patient for transplantation. METHODS:Survival without OLT, improvement in liver function and ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(01)00228-8
更新日期：2002-01-01 00:00:00
abstract：:Intracellular ion homeostasis in liver cell is accomplished through the concerted action of ion pumps, carriers and channels. Description of these elements includes the Na+/K+-ATPase, Na+/H+- and Cl-/ HCO3(-)-exchange and Na+-HCO-3(-)- and Na(+)-K(+)-2 Cl(-)-cotransport as well as K+-, Cl(-)-, Na+ and nonselective cat...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND/AIMS:As conditional knockout mice for stat3 are impaired in liver regeneration after partial hepatectomy while those for gp130 have defects in early STAT3 phosphorylation but have normal DNA synthesis, late STAT3 phosphorylation induced independently of gp130 seems to be essential for liver regeneration. Sin...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2007.08.020
更新日期：2008-02-01 00:00:00
abstract：BACKGROUND & AIMS:Genetic variants of patatin-like phospholipase domain-containing 3 (PNPLA3) and diabetes are associated with liver disease severity, in patients with chronic hepatitis C (CHC) infection. We aimed at exploring their interaction in determining hepatitis C virus (HCV)-related liver fibrosis. METHODS:The...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2014.10.011
更新日期：2015-03-01 00:00:00
abstract：BACKGROUND & AIMS:Liver transplant (LT) patients might be overimmunosuppressed as recommendations for tacrolimus trough concentrations (TC) within 4-6 weeks after liver transplantation are set too high (10-15 ng/ml). Early tacrolimus exposure was evaluated in relation to acute rejection and long-term outcomes. METHODS...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2012.09.019
更新日期：2013-02-01 00:00:00
abstract：BACKGROUND/AIM:Smooth muscle antibodies and antinuclear antibodies characterize type 1 autoimmune hepatitis. Our aim was to correlate the appearance and disappearance of these autoantibodies with clinical and histological events. METHODS:One hundred and seven patients were evaluated successively over 128+/-9 months. A...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80096-8
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND/AIMS:Pregnant women with acute viral hepatitis (VH) and those with pregnancy associated acute liver disease (PAALD) including acute fatty liver disease of pregnancy, hemolysis elevated liver enzyme and low platelet syndrome present with similar clinical features and liver tests abnormalities. Accurate differ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2008.07.030
更新日期：2008-12-01 00:00:00
abstract：AIMS:To assess the presence of autonomic neuropathy in patients with non-alcoholic chronic liver disease and its relationships with the severity of liver damage. METHODS:Thirty non-alcoholic patients with chronic liver disease and 26 healthy control subjects were studied. The silicone imprint technique was used to qua...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80458-8
更新日期：1997-06-01 00:00:00
abstract：:Surveillance of patients at risk of developing hepatocellular carcinoma (HCC) is based on ultrasound (US) examinations performed at either 6 or 12 month intervals. Early detection of HCC in patients with cirrhosis is a challenging issue, since the different entities that are involved in the multi-step process of hepat...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2008.02.005
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND & AIMS:Clinically significant portal hypertension (CSPH), defined as a hepatic venous pressure gradient (HVPG) ≥10 mmHg, persists 24 weeks after sustained virological response (SVR) in up to 78% of patients with HCV-related cirrhosis treated with direct-acting antivirals. These patients remain at risk of dec...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2020.05.050
更新日期：2020-12-01 00:00:00
abstract：:The mechanism responsible for liver cell necrosis in patients with hepatitis A is not known. Since the type of hepatic lesions and the clinical presentation of acute hepatitis B are similar and are probably related to the cell-mediated immune response to a viral antigen located in the liver cell, it is possible that a...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(86)80082-4
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND/AIMS:Cirrhotic patients chronically treated with beta-blockers who achieve a decrease of hepatic venous pressure gradient (HVPG) > or =20% from baseline or to < or =12 mmHg have a marked reduction of first bleeding or re-bleeding. However, two HVPG measurements are needed to evaluate response. This study was...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2009.04.015
更新日期：2009-08-01 00:00:00
abstract：:This study evaluates systemic and splanchnic haemodynamics and the effect of propranolol in 15 patients with presinusoidal portal hypertension (portal vein obstruction, n = 11; schistosomiasis, n = 4). These patients exhibited a hyperkinetic circulatory syndrome characterized by high cardiac index (4.4 +/- 1.61.min-1....
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(89)90139-6
更新日期：1989-11-01 00:00:00
abstract：BACKGROUND/AIMS:The etiopathogenesis of bile duct lesion in primary biliary cirrhosis is unknown, though the participation of bacteria and/or their components and products is suspected. In this study, we tried to detect and identify bacteria in the bile of patients with primary biliary cirrhosis by polymerase chain rea...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(00)80153-1
更新日期：2000-07-01 00:00:00
abstract：:Short- and long-term effects of intraperitoneally transplanted microcarrier attached liver cells (MAL) have been studied in two experimental models of severe liver insufficiency in the rat: subtotal hepatectomy (HX) and acute liver ischemia. Intraperitoneal transplantation of MAL immediately after subtotal hepatectomy...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(89)90075-5
更新日期：1989-07-01 00:00:00
abstract：:All routinely available assays to detect hepatitis C virus (HCV) infection are based on the specific reaction of serum antibodies to corresponding viral antigens. The only method to detect viral nucleic acids in serum or liver is the enzymatic amplification of reversely transcribed HCV-RNA by polymerase chain reaction...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(92)90074-y
更新日期：1992-07-01 00:00:00
abstract：BACKGROUND/AIMS:Members of the melanoma antigen encoding gene family are expressed in tumors of different histological types but not in normal tissue. For this reason, they are attractive targets for cancer immunotherapy. METHODS:In the present study, we analyzed the expression of MAGE-1 and -3 genes in the hepatocell...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00484-7
更新日期：2004-01-01 00:00:00
abstract：:Porphyria cutanea tarda (PCT) is a metabolic disorder characterized by a reduced hepatic activity of uroporphynogen decarboxylase (URO-D), an enzyme of the heme synthesis. The clinical features of PCT may be brought into light by hepatic injury induced by hepatitis C virus (HCV). A significant association between HCV ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2004.10.029
更新日期：2005-04-01 00:00:00
abstract：:The circuitous cell signalling pathways of hepatocytes comprise several factors that operate to downgrade or even interrupt the transmission of a given signal. These down-regulating influences are essential to keep cell proliferation and cell survival in check and if impaired, can alter a delicate balance in favour of...
journal_title：Journal of hepatology
pub_type：
doi：10.1016/j.jhep.2007.09.003
更新日期：2007-12-01 00:00:00
abstract：:Clinical complexity, anatomic diversity and molecular heterogeneity of cholangiocarcinoma (CCA) represent a major challenge in the assessment of effective targeted therapies. Molecular and cellular mechanisms underlying the diversity of CCA growth patterns remain a key issue of clinical concern. Crucial questions comp...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2014.09.007
更新日期：2015-01-01 00:00:00
abstract：:Medical treatment of cirrhotic ascites is essentially supportive, dictated by the patient's discomfort, impaired cardiovascular or respiratory function and potential for infection. Treatment of 'simple' ascites (moderate fluid accumulation, serum albumin > 3.5 g/dl, serum creatinine < 1.5 mg/dl, no electrolyte disturb...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/s0168-8278(05)80447-7
更新日期：1993-01-01 00:00:00
abstract：:The influence of hepatitis C virus and its subtypes on the clinical course of liver disease in alcoholics was assessed. Hepatitis C virus infection was confirmed by a reverse transcription and polymerase chain reaction method for the hepatitis C virus NS-5 region in the sera of alcoholics with various stages of histol...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(95)80419-6
更新日期：1995-02-01 00:00:00
abstract：BACKGROUND & AIMS:It is established that cirrhotic patients who respond to beta-blockers by lowering their hepatic venous pressure gradient (HVPG) to < or =12 mmHg or by > or =20% of the baseline values are protected from bleeding. However, it is not known whether the effect remains unchanged over the treatment period....
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2009.10.014
更新日期：2010-01-01 00:00:00
