Detection of myosin gene expression in the developing heart using probes derived by polymerase chain reaction.

Abstract:

:The polymerase chain reaction provides a rapid method for the molecular cloning of DNA probes suitable for the detection of specific messenger RNA. We have used this approach to prepare probes specific for human cardiac myosin messenger RNA and demonstrate here the use of such probes in the analysis of human cardiac development by hybridization in situ to sections of fetal tissue. This combination of techniques is suitable for the detection of any messenger RNA for which sequence data are available, and offers a powerful new approach to the analysis of cardiac development.

journal_name

Int J Cardiol

authors

Barton PJ,Moscoso G,Thompson RP

doi

10.1016/0167-5273(91)90133-a

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

116-8

issue

1

eissn

0167-5273

issn

1874-1754

pii

0167-5273(91)90133-A

journal_volume

30

pub_type

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