Abstract：
:The cercariae stages of Prosorhynchus crucibulum and Prosorhynchus aculeatus are morphologically indistinguishable. However, the differentiation of these two species is crucial to understand the transmission dynamics between these primary hosts (mussels) and the secondary hosts (fish). In this way, the objective of this study is to develop an accurate molecular identification tool to differentiate the cercariae stage of P. crucibulum and P. aculeatus. We targeted the 18S nuclear ribosomal DNA region by PCR amplification and sequenced this amplicon. By generating these sequences, we developed a RFLP tool with the use of the enzymes HincII and FokI that produced different restriction profiles between P. crucibulum and P. aculeatus. Each enzyme generated different-sized fragments specific to the species examined and no cross-reaction between the species was detected in their restriction pattern. By sequencing, no intraspecific-polymorphism was detected since there is 100% homology among P. aculeatus or P. crucibulum. These results indicate that PCR-linked restriction analysis of the 18S rDNA region provided us with rapid and reliable molecular tools for distinction of the cercariae of these species.
journal_name
Parasitol Intjournal_title
Parasitology internationalauthors
Francisco CJ,Almeida A,Castro AM,Santos MJdoi
10.1016/j.parint.2009.09.004subject
Has Abstractpub_date
2010-03-01 00:00:00pages
40-3issue
1eissn
1383-5769issn
1873-0329pii
S1383-5769(09)00114-7journal_volume
59pub_type
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journal_title：Parasitology international
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pub_type： 杂志文章,评审
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journal_title：Parasitology international
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章
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journal_title：Parasitology international
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