Abstract:
:Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple pathological conditions. Therefore, targeting these enzymes may be important for a better understanding of their function and development of therapeutic inhibitors. Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidases specificity as they allow monitoring of the reaction on a continuous basis, providing a rapid method for the determination of enzymatic activity. Hydrolysis of a peptide bond between the donor/acceptor pair generates fluorescence that permits the measurement of the activity of nanomolar concentrations of the enzyme. The assays can be performed directly in a cuvette of the fluorimeter or adapted for determinations in a 96-well fluorescence plate reader. The synthesis of FRET peptides containing ortho-aminobenzoic acid (Abz) as fluorescent group and 2, 4-dinitrophenyl (Dnp) or N-(2, 4-dinitrophenyl)ethylenediamine (EDDnp) as quencher was optimized by our group and became an important line of research at the Department of Biophysics of the Federal University of São Paulo. Recently, Abz/Dnp FRET peptide libraries were developed allowing high-throughput screening of peptidases substrate specificity. This review presents the consolidation of our research activities undertaken between 1993 and 2008 on the synthesis of peptides and study of peptidases specificities.
journal_name
An Acad Bras Ciencjournal_title
Anais da Academia Brasileira de Cienciasauthors
Carmona AK,Juliano MA,Juliano Ldoi
10.1590/s0001-37652009000300005subject
Has Abstractpub_date
2009-09-01 00:00:00pages
381-92issue
3eissn
0001-3765issn
1678-2690pii
S0001-37652009000300005journal_volume
81pub_type
杂志文章,评审abstract::The objective was to evaluate the influence of calving and postpartum characteristics, measured in different genetic predominance, on the postpartum rebreeding. Were evaluated 437 partum from cows that received feeding management consisting of a forage base of the native field and the mating season consisted of 90 day...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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doi:10.1590/s0001-37652009000400013
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章,评审
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
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