Epithelial and PGP9.5-immunoreactive cells of Malassez epithelium in the periodontal ligament of cats: a transmission electron microscopic study.

Abstract:

OBJECTIVE:The aim of the study was to investigate the ultrastructural features of Malassez epithelium (ME) containing protein gene product 9.5 (PGP9.5)-immunoreactive (IR) cells in the cat periodontal ligament (PDL). MATERIAL AND METHODS:Specimens from the teeth and tooth-supporting tissues of four mature cats of both sexes, 18 to 24 months of age, were used. The fixed jaws were decalcified in EDTA. Frozen sagittal sections 20 µm thick were immunostained for PGP9.5, and the ME, containing IR cells in the PDL, were evaluated under a transmission electron microscope. RESULTS:Several epithelial cells and PGP9.5 IR cells formed clusters and were enveloped by a basal lamina and separated from the surrounding connective tissue. A large nucleus and scanty cytoplasm were observed in most of the ME cells, which contained abundant keratin filaments and mitochondria. Caveolae-like structures and vesicles were found in the periphery of the ME. The small cytoplasmic processes of some of the epithelial cells extended toward the surrounding connective tissues. The cytoplasmic matrix of one type of cell comprising the ME showed immunoreactivity for anti-PGP9.5 antibody. The IR cell in the cell clusters was connected to adjacent epithelial cells and extended cytoplasmic processes toward the adjacent epithelial cells. The IR cell contained keratin filaments and abundant densely cored vesicles approximately 100-250 nm in diameter. CONCLUSIONS:The findings of the study suggest endocytotic capabilities of the epithelial cells and neuroendocrine functions of the IR cells. It is possible that the two different cell types react to extrinsic stimuli and interact with cells comprising the clusters and cords in the PDL. These ultrastructural evidences may imply functional heterogeneity of the ME in the PDL.

journal_name

Acta Odontol Scand

authors

Tadokoro O

doi

10.1080/00016350903150002

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

388-92

issue

6

eissn

0001-6357

issn

1502-3850

pii

914173600

journal_volume

67

pub_type

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