Abstract:
OBJECTIVE:Gingival fibroblasts (GFs) produce pro-inflammatory cytokines in response to stimulation with lipopolysaccharide (LPS) of Porphyromonas gingivalis, which is thought to be mediated by activation of toll-like receptors (TLR)2 and TLR4. The present study investigated the expression of interleukin (IL)-6, TLR2, and TLR4 in GFs of seven different donors upon stimulation with P. gingivalis LPS. The effects of P. gingivalis LPS were compared with those of TLR4 agonist Escherichia coli LPS and TLR2 agonist Pam3CSK4. MATERIALS AND METHODS:GFs were stimulated with P. gingivalis LPS, E. coli LPS or Pam3CSK4 and the expression of IL-6, TLR2 and TLR4 was measured by qPCR. The surface expression of TLR2 and TLR4 was measured by flow cytometry. RESULTS:In GFs from three donors, P. gingivalis LPS and Pam3CSK4 induced a markedly lower increase in IL-6 expression than E. coli LPS. This was accompanied by significant down-regulation of the TLR2 and TLR4 expression. In GFs from another four donors, an increase in IL-6 expression upon stimulation with P. gingivalis LPS and Pam3CSK4 was similar or even higher than that induced by E. coli LPS. In GFs of these donors, all stimuli induced an up-regulation of both mRNA and protein expression of TLR2 and did not influence that of TLR4. CONCLUSIONS:This study suggests that P. gingivalis LPS and E. coli LPS differently regulate cytokine production in human gingival fibroblasts. Regulation of the expression level of TLR2 and TLR4 by periodontal pathogens might be an important factor controlling the inflammatory response in GFs.
journal_name
Acta Odontol Scandjournal_title
Acta odontologica Scandinavicaauthors
Andrukhov O,Ertlschweiger S,Moritz A,Bantleon HP,Rausch-Fan Xdoi
10.3109/00016357.2013.834535subject
Has Abstractpub_date
2014-07-01 00:00:00pages
337-45issue
5eissn
0001-6357issn
1502-3850journal_volume
72pub_type
杂志文章abstract::The purpose was to study differences in the caries increment rate as influenced by various sugars. The trial involved almost complete substitution of sucrose (S) by fructose (F) or xylitol (X) during a period of 2 years. There were no significant initial differences as to caries status between the prospective sugar gr...
journal_title:Acta odontologica Scandinavica
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/00016357608997711
更新日期:1976-01-01 00:00:00
abstract::The efficacy of oral fluconazole versus nystatin was evaluated as a treatment modality for oral candidosis. Of the included patients (n = 60), two-thirds presented with an erythematous candidosis, and the others showed clinical signs compatible with a pseudomembranous candidosis. Predisposing factors were xerostomia (...
journal_title:Acta odontologica Scandinavica
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/00016359850142790
更新日期:1998-08-01 00:00:00
abstract::Three composites, two microfilled (Heliosit and Silux) and one macrofilled (Prisma-Fil), were exposed to visible light from three different light units. The depth of the cure was estimated by measuring the length of the test specimen after removing the uncured material. The macrofilled composite cured to a greater dep...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016358409041127
更新日期:1984-02-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of residual calcium hydroxide [Ca(OH)2] on the accuracy of an electronic apex locator (EAL). MATERIALS AND METHODS:Working lengths (WLs) of 56 extracted maxillary incisors were determined initially by two different methods. The first method used the 'APEX' rea...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2014.961027
更新日期:2015-02-01 00:00:00
abstract::In a previous study, dentists reported very high scores for perceived physical workload, but only low to moderate scores for the musculoskeletal system. This is difficult to explain when other occupational groups in the dental services are compared, and is the main reason why the present study was performed. To measur...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.1080/00016350510019964
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the surface roughness of IPS Empress 2 ceramic when treated with different finishing/polishing protocols. MATERIALS AND METHODS:Sixteen specimens of IPS Empress 2 ceramic were made from wax patterns obtained using a stainless steel split mold. The specimens were glazed (Stage 0-S0, control) and d...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2012.690570
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVE:A major aspect in evaluating the quality of dental materials is their physical properties. Their properties should be a best fit of the ones of dental hard tissues. Manufacturers give data sheets for each material. The properties listed are characterized by a specific value. This assumes (but does not prove) ...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2013.878391
更新日期:2014-11-01 00:00:00
abstract::Several physical properties such as strength (UTS and fracture and proof stress), modulus of elasticity, strain (elastic and plastic elongation), hardness, and hardening ability were determined for five low-gold alloys and two conventional gold casting alloys (types III and IV). The composition and the structure (pore...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016358309162310
更新日期:1983-01-01 00:00:00
abstract::The enzyme beta-glucuronidase (E.C. 3.2.1.31.) has been studied in developing teeth and bone of Sprague-Dawley rats and Macaque monkeys (Macaca Irus) by means of histochemistry using naphthol-AS-BI-beta-D-glucuronide as substrate and hexazotized pararosanilin as coupling reagent. Since beta-glucuronidase is sensitive ...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357509026357
更新日期:1975-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effects of various irrigating solutions and photoactivated disinfection (PAD) on the push-out bond strengths of fiber posts to root dentin. MATERIALS AND METHODS:Thirty-two human teeth were divided into eight groups, as follows: (1) irrigation with physiologic saline...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2014.906651
更新日期:2014-11-01 00:00:00
abstract::A 55Fe excited X-ray fluorescence method has been developed for the quantitative nondestructive analysis of Ca content in surface layers of teeth. To eliminate the effects due to curved enamel surface and the localisation of the tooth a special measuring geometry was constructed. The reproducibility of the method rema...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016358209041119
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVES:To study the dental fluorosis and caries in the permanent teeth of 12 to 13-year-old children in fluorosis-endemic areas; to assess the relationship between fluorosis and the fluoride content of the drinking water and the relationship between caries and the fluoride content of the water; finally, to analyze ...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.1080/00016350510019991
更新日期:2005-10-01 00:00:00
abstract::Twenty patients complaining of symptoms deriving from their amalgam fillings and a non-patient group were assessed by means of the perceptual projective Defense Mechanism Test (DMT). The test protocols were scored for 130 DMT variables and analyzed by means of the multivariate statistical method Partial Least Squares ...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016359609003520
更新日期:1996-06-01 00:00:00
abstract::Signs and symptoms of temporomandibular disorders (TMDs) and the frequency of radiologically observed abnormalities in the condyles of temporomandibular joints (TMJs) of adolescent violin players (VP group) were investigated in a group of 31 music students and in their age- and sex-matched controls (C group). All subj...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016359609003536
更新日期:1996-08-01 00:00:00
abstract:OBJECTIVE:To investigate the associations between oral sugar clearance and the prevalence of dental decay. MATERIAL AND METHODS:A total of 92 (44 F, 48 M) 71-year-old subjects in Goteborg, Sweden were consecutively chosen from a representative cohort study. The subjects were examined for: 1) caries-related status, 2) ...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.1080/00016350802378647
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVE:To use ultrasonic measurements to monitor the effects of adhesive application and power density on the polymerization behavior of dual-cured core build-up resins. METHODS:Ultrasonic measurements were carried out using a pulser-receiver, transducers and an oscilloscope. The core build-up resins were mixed, in...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2011.654252
更新日期:2013-01-01 00:00:00
abstract::According to several studies on human populations, the mandible deviates on opening significantly more often to the left than to the right. An association between the asymmetry of opening and handedness has been suggested. The present study of 143 left-handed Finnish conscripts showed no significant difference from th...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章,评审
doi:10.3109/00016358909167309
更新日期:1989-04-01 00:00:00
abstract::Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolmethacrylate. Ultrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus gamma-globulin. It was found that unfixed specimens became labelled as p...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016358209024071
更新日期:1982-01-01 00:00:00
abstract::Proton microprobe analysis was used to determine the fluoride concentrations in enamel and dentin surfaces exposed to a fluoride-containing and a conventional amalgam in vitro. After seven weeks of exposure, a considerable fluoride uptake was demonstrated from the fluoride-containing amalgam. The fluoride deposition w...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016358009033594
更新日期:1980-01-01 00:00:00
abstract:OBJECTIVE:It should be expected that type 1 diabetes mellitus may disturb innate and acquired immunity. There are no data on type 1 diabetes mellitus-related changes in the salivary flow and the protein output responsible for the innate immunity of saliva depending on the quality of dentition reflecting the age of chil...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2013.773071
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To investigate the incidence and prevalence of developmental odontogenic cysts in children and adolescents and compare the features of the two most common types, dentigerous cyst and keratocystic odontogenic tumor (KCOT). STUDY DESIGN:A retrospective review in a series of 369 patients with all histological d...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2014.913192
更新日期:2014-11-01 00:00:00
abstract::The adolescent growth of 212 randomly selected Swedish urban children has been investigated by using a graphical analysis of unsmoothed increments of height. The adolescent growth period has been divided into the pubertal spurt and the postpubertal period. On average, the pubertal spurt began at 10.0 years in girls, a...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016358008997719
更新日期:1980-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of age on root resorption and distribution along different parts of the root during prolonged light force application. MATERIAL AND METHODS:Orthodontic appliances were placed in two groups of 30 rats (one group 6 weeks old, the other 9-12 months old), with contralateral sides as con...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.1080/00016350801982522
更新日期:2008-04-01 00:00:00
abstract::The longevity of amalgam restorations in premolars and first and second molars in long-term recall patients has been calculated by means of survival analysis. The present retrospective longitudinal study includes information from 32 patients and their records over a time period of 17 years. The data were recorded by s...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016359009005863
更新日期:1990-04-01 00:00:00
abstract::The aim of the study was to compare the uptake of the trace metals lead, cadmium, zinc and copper, in deciduous and permanent teeth. The material consisted of teeth extracted in the Oslo area. The teeth were digested in hydrochloric acid, and analyses were made by differential pulse stripping voltammetry. Similar leve...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357609002559
更新日期:1976-01-01 00:00:00
abstract::Seventy-three children with submucous cleft palate (38 girls and 35 boys), mean age 8.2 years (range 7.7-9.5), were studied retrospectively from orthopantomograms. Dental abnormalities in permanent dentition were found in 26 patients (36%). Missing teeth, mainly lower 2nd premolars, upper lateral incisors, and upper 2...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.1080/00016350410006572
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVE:The aim of the current study was to investigate the presence of sulphate-reducing bacteria (SRB) in human saliva and correlate with oral and systemic conditions. METHODS:Saliva samples were collected from 118 patients and inoculated in 2 ml of modified Postgate's E medium culture. After 28 days of incubation...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2013.770163
更新日期:2013-11-01 00:00:00
abstract::Microleakage was studied in class-II cavities restored with a calcium hydroxide liner and an adhesive system combined with two different posterior composite resins. The restorations were exposed to repeated loading when immersed in dye solution. The teeth were cut, and microleakage along the cavity walls and into dent...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016359109005915
更新日期:1991-08-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to analyze the clinicopathological and immunohistochemical features of oral papillomas. MATERIALS AND METHODS:Biopsies of oral papillomas analyzed in the laboratory between 1996-2012 were extracted from the database and used to conduct this retrospective review. The following clinic...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.3109/00016357.2014.996186
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:To present a follow-up using the Dental Discomfort Questionnaire (DDQ) before and after the treatment of children, under the assumption that the number of toothache-related behaviors diminishes as a result of treatment, and to see whether this effect is related to the site of the carious teeth or to the treat...
journal_title:Acta odontologica Scandinavica
pub_type: 杂志文章
doi:10.1080/00016350500264362
更新日期:2005-11-01 00:00:00