Abstract：
BACKGROUND:Sindbis virus (SIN) infection causes no or only mild symptoms (fever, rash, and arthralgia) in humans. However, SIN has a strong cytopathic effect (CPE) on various cancer cells. This study focuses on the oncolytic activity of SIN AR399 on oral cancer cells compared with reovirus, a well-known oncolytic virus that targets cancer cells. METHODS:We analysed the cytotoxicity and growth of SIN in 13 oral squamous cell carcinoma (OSCC) cell lines (HSC-2, HSC-3, HSC-4, Ca9-22, H-1, Sa-3, KON, KOSC-2, OK-92, HO-1-N1, SCC-4, SAT, SKN-3) and normal human oral keratinocytes (NHOKs). RESULTS:Sindbis virus infection induced CPE in 12 OSCC cell lines at a low multiplicity of infection (MOI) of 0.01, but not in the OSCC cell line, HSC-4 or NHOKs. Sindbis viral growth was not observed in NHOKs, whereas high SIN growth was observed in all OSCC cell lines, including HCS-4. The cytotoxicity and growth of SIN was the same as reovirus at an MOI of 20 in 12 OSCC cell lines. The CPE was shown, by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling assays, to be apoptotic cell death. Furthermore, quantitative RT-PCR of mRNA in HSC-3 and HSC-4 cells after SIN infection showed that activation of caspases, cytochrome c, and IkappaBalpha was associated with SIN-induced apoptosis. CONCLUSION:As a replication-competent oncolytic virus, SIN may be a useful therapeutic modality for oral cancers.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Saito K,Uzawa K,Kasamatsu A,Shinozuka K,Sakuma K,Yamatoji M,Shiiba M,Shino Y,Shirasawa H,Tanzawa Hdoi
10.1038/sj.bjc.6605209subject
Has Abstractpub_date
2009-08-18 00:00:00pages
684-90issue
4eissn
0007-0920issn
1532-1827pii
6605209journal_volume
101pub_type
杂志文章abstract：:Although CD133 has been shown to be a marker for cancer stem cells in various tumours, its expression in pancreatic cancer has not yet been clinically reported. In this study, we investigated the relationship between CD133 expression and clinicopathological factors in pancreatic cancer. Pancreatic head carcinoma speci...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604307
更新日期：2008-04-22 00:00:00
abstract：BACKGROUND:Gold(III) porphyrin 1a is a new class of anticancer drug, which inhibits cell proliferation of wide range of human cancer cell lines and induces apoptosis in human nasopharyngeal carcinoma cells. However, the underlying signalling mechanism by which gold(III) porphyrin 1a modifies the intracellular apoptosis...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605147
更新日期：2009-07-21 00:00:00
abstract：:In a phase I study of weekly administered cisplatin combined with oral etoposide, we observed a partial response in 4 out of 11 patients with metastatic colorectal cancer. Subsequently, we performed a phase II study to investigate the activity of this combination as first-line treatment in this disease. Fourteen patie...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1996.242
更新日期：1996-05-01 00:00:00
abstract：BACKGROUND:Several tyrosine kinase inhibitors (TKIs) can decrease docetaxel clearance in patients by an unknown mechanism. We hypothesised that these interactions are mediated by the hepatic uptake transporter OATP1B1. METHODS:The influence of 16 approved TKIs on transport was studied in vitro using HEK293 cells expre...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.811
更新日期：2014-02-18 00:00:00
abstract：:The evidence base for head and neck cancers is low with relatively few randomized controlled trials of the two main treatments, surgery and radiotherapy. The aim of the study was to investigate the patterns of surgery and radiotherapy treatment for head and neck cancers in three large areas of England and to investiga...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690695
更新日期：1999-09-01 00:00:00
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journal_title：British journal of cancer
pub_type： 杂志文章,meta分析,多中心研究
doi：10.1038/sj.bjc.6604838
更新日期：2009-01-27 00:00:00
abstract：:To determine the maximum-tolerated dose (MTD), dose-limiting toxicities and pharmacokinetic of semisynthetic homoharringtonine (ssHHT), given as a twice daily subcutaneous (s.c.) injections for 9 days, in patients with advanced acute leukaemia, 18 patients with advanced acute myeloid leukaemia were included in this se...
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pub_type： 杂志文章,随机对照试验
doi：10.1038/sj.bjc.6603265
更新日期：2006-08-07 00:00:00
abstract：:Dynamic contrast-enhanced MR imaging (DCE-MRI) may act as a biomarker for successful cancer therapy. Simple, reproducible techniques may widen this application. This paper demonstrates a single slice imaging technique. The image acquisition is performed in less than 500 ms making it relatively insensitive to respirato...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603140
更新日期：2006-05-22 00:00:00
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journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.568
更新日期：1998-09-01 00:00:00
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journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.644
更新日期：2013-11-12 00:00:00
abstract：:We investigated the relation between head and neck cancer risk and alcohol consumption in the NIH-AARP Diet and Health Study. During 2,203,500 person-years of follow-up, 611 men and 183 women developed head and neck cancer. With moderate drinking (up to one alcoholic drink per day) as the referent group, non-drinkers ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603713
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abstract：:Comorbid diseases can affect breast cancer prognosis. We conducted a population-based study of Danish women diagnosed with a first primary breast cancer from 1995 to 2005 (n=9300), using hospital discharge registry data to quantify comorbidities by Charlson score. We examined the influence of comorbidities on survival...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/sj.bjc.6603717
更新日期：2007-05-07 00:00:00
abstract：:We screened 145 HIV-infected non-pregnant women at a tertiary care centre in Lusaka, Zambia. Liquid-based cytology and human papillomavirus (HPV) genotyping with PGMY09/11 biotinylated primers (Roche Linear Array HPV genotyping test) maximised sensitivity of cytology and HPV assessments. Among high-risk (HR) types, HP...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603737
更新日期：2007-05-07 00:00:00
abstract：:The radiation response of Epstein-Barr virus (EBV)-immortalised lymphoblastoid cell lines derive from Li-Fraumeni syndrome (LFS) and LFS-like individuals was investigated. Cells from all LFS and LFS-like cases showed an accumulation of p53 protein following 137Cs gamma-irradiation, which was associated with cell cycle...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.424
更新日期：1996-09-01 00:00:00
abstract：:In the UK, 10% of patients diagnosed with rectal cancer have inoperable disease at presentation. This study ascertained whether the resectability rate of inoperable locally advanced rectal cancer was improved by administration of intravenous irinotecan, 5-fluorouracil (5-FU) and pelvic radiotherapy. During phase I of ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604292
更新日期：2008-04-08 00:00:00
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journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.405
更新日期：2012-10-23 00:00:00
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journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.134
更新日期：1993-04-01 00:00:00
abstract：:The retinoblastoma gene product (RB) contributes to normal cell growth control. One of its functions is manifest as a block to exit from G1, which is carried out by an RB subspecies which is un- or underphosphorylated. After RB phosphorylation, a process which occurs towards the end of G1 in cycling cells, the block i...
journal_title：British journal of cancer
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doi：10.1038/bjc.1993.325
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journal_title：British journal of cancer
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journal_title：British journal of cancer
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journal_title：British journal of cancer
pub_type： 杂志文章
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更新日期：1977-12-01 00:00:00
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doi：10.1038/sj.bjc.6601766
更新日期：2004-05-04 00:00:00
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pub_type： 杂志文章,评审
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journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690108
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abstract：BACKGROUND:The Mediterranean diet has been shown to have a beneficial role on various neoplasms, but data are scanty on pancreatic cancer. METHODS:We analysed data from two case-control studies conducted in Italy between 1983 and 2008, including 362 and 326 pancreatic cancer cases and 1552 and 652 hospital-controls, r...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.345
更新日期：2013-09-03 00:00:00
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pub_type： 杂志文章
doi：10.1038/bjc.2014.438
更新日期：2014-09-09 00:00:00
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journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/sj.bjc.6605588
更新日期：2010-03-16 00:00:00
abstract：:We examined a panel of 25 small cell lung cancer (SCLC) cell lines and nude mouse xenografts for expression of the proto-oncogenes c-met and c-kit, and for expression of the corresponding ligands, hepatocyte growth factor (HGF) (also known as scatter factor (SF)), and stem cell factor (SCF), respectively. Expression o...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.7
更新日期：1993-01-01 00:00:00
abstract：:This study sought to examine changes in psychological distress following cancer genetic counselling. Women attending a family cancer clinic completed questionnaires before their appointment and at 2 weeks, 6 months and 12 months after their appointment. Twenty-six women were at low risk of developing breast or ovarian...
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pub_type： 杂志文章
doi：10.1038/sj.bjc.6600030
更新日期：2002-01-07 00:00:00
