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Sequence specific primer extension RNA analysis (SeSPERA) for the investigation of substrate utilization of microbial communities.

Abstract：

:A simple, fast and cost-effective method for the taxon-specific separation of various 16S rRNAs was developed. This primer extension mediated separation of different RNA species was obtained on agarose gels. The method provides an easy way for the investigation of substrate mediated radioisotope incorporation into the RNA.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Franchini AG,Nikolausz M,Kästner M

doi

10.1016/j.mimet.2009.07.010

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

111-3

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(09)00223-1

journal_volume

79

pub_type

杂志文章

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