Abstract:
:A method for the detection of bacterial pathogens in sepsis and bacterial meningitis with 16S rRNA gene- based real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) is developed. A total of 190 blood specimens and 5 cerebrospinal fluid specimens from neonates with suspected sepsis or bacterial meningitis were evaluated with 16S rRNA gene-based real-time FQ-PCR assay. The positive rate of the real-time FQ-PCR assay was significantly higher (25/195, 12.82%) than that of bacterial culture (15/195, 7.69%; P = .002). When bacterial culture was used as a control, the sensitivity of the real-time FQ-PCR was 100%, the specificity was 94.4%, and Youden's index was 0.944. This study suggests that 16S rRNA gene-based real-time FQ-PCR assay is an important and accurate method in the detection of bacterial pathogens of sepsis and bacterial meningitis and should have a promising usage in the diagnosis of sepsis and bacterial meningitis.
journal_name
Clin Pediatr (Phila)journal_title
Clinical pediatricsauthors
Chen LH,Duan QJ,Cai MT,Wu YD,Shang SQdoi
10.1177/0009922809333972subject
Has Abstractpub_date
2009-07-01 00:00:00pages
641-7issue
6eissn
0009-9228issn
1938-2707pii
0009922809333972journal_volume
48pub_type
杂志文章abstract::OBJECTIVES. Cough, pain, and desaturation episodes in infants are often ascribed to gastroesophageal reflux, and many are empirically treated with acid suppression medications. The authors hypothesize that most of these symptoms are not related to gastroesophageal reflux. METHODS. Retrospective review of 186 combined ...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922811412585
更新日期:2011-12-01 00:00:00
abstract::Three hundred eighty-six isolates of Propionibacterium sp. were recovered from 2,003 specimens studied for the identification of anaerobic bacteria in children during a 15-year period. Three hundred forty-three (89%) of these were Propionibacterium acnes. A total of 50 (13%) Propionibacterium isolates identified from ...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992289403300807
更新日期:1994-08-01 00:00:00
abstract::A 6-year-old girl with sickle cell disease was admitted to the hospital with the diagnosis of the acute chest syndrome. The laboratory findings and the radionuclear lung scan supported a diagnosis of pulmonary infarction rather than pneumonia. She improved with intravenous fluids, oxygen, penicillin, and theophylline....
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288502401209
更新日期:1985-12-01 00:00:00
abstract::Anemia is defined as a reduction in red blood cell mass or hemoglobin concentration in blood. It is statistically differentiated from normal states as a hemoglobin concentration or hematocrit 2 SD below the mean for the healthy population adjusted for age and sex. Anemias may be classified based on the morphologic fun...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922808323115
更新日期:2009-01-01 00:00:00
abstract::The aim of this integrative review is to investigate current literature regarding family health history (FHH) taking practices, attitudes, and challenges in the pediatric outpatient setting. FHH is a known clinical tool for providers; however, there are no explicit standards for pediatric FHH collection. The integrati...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922820943474
更新日期:2020-12-01 00:00:00
abstract::This study examines primary care provider (PCP) experiences with the initial parental disclosure of cystic fibrosis (CF) newborn screening (NBS) results in order to identify methods to improve parent-provider communication during the CF NBS process. PCPs of infants who received positive CF NBS results participated in ...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922814545619
更新日期:2015-01-01 00:00:00
abstract::The General Academic Pediatrician (GAP) may act as a consultant to both primary care pediatricians and tertiary care subspecialists. The authors surveyed a consultation service staffed by three GAP's in a tertiary care children's hospital. The service was financially successful and generated new sources of referrals f...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288802701203
更新日期:1988-12-01 00:00:00
abstract::The purpose of this study was to compare the bacteriologic and clinical efficacy of oral penicillin versus amoxicillin as first-line therapy for group A beta-hemolytic streptococcal (GABHS) tonsillopharyngitis. The prospective observational study was conducted over 18 months (January 2000-June 2001). Children enrolled...
journal_title:Clinical pediatrics
pub_type: 临床试验,杂志文章,评审
doi:10.1177/000992280304200305
更新日期:2003-04-01 00:00:00
abstract::A 4-year-old boy with transient erythroblastopenia of childhood (TEC) presented with papilledema and transient hemiparesis. Upon spontaneous hematologic recovery, the papilledema resolved. It is concluded that TEC, like other forms of anemia, may present with papilledema and even focal neurologic deficit, from which s...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288602500510
更新日期:1986-05-01 00:00:00
abstract:OBJECTIVE:This study describes the clinical course, treatment, and outcome of 13 critically ill children due to infection with new influenza A H1N1, admitted to 2 pediatric intensive care units (PICUs) in the northwestern part of the Netherlands. METHODS:Retrospective case series, conducted in 2 PICUs in Amsterdam, th...
journal_title:Clinical pediatrics
pub_type: 杂志文章,多中心研究
doi:10.1177/0009922810381426
更新日期:2011-01-01 00:00:00
abstract::Developmental language disorder has been reported in 3% to 10% of term infants and 30% of preterm infants (<34 weeks gestation). Screening for language delay in preterm infants can be costly and time-consuming. The objective of this study was to assess the expressive language development of preterm infants using the L...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922812439244
更新日期:2012-07-01 00:00:00
abstract::Pediatrician reimbursement is shifting from fee-for-service to a fixed salary. In the Netherlands, as physicians working on a fee-for-service basis have a financial interest in talking less and in carrying out more diagnostic tests and investigations, it may be questioned whether this will influence the structure and ...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992280003901004
更新日期:2000-10-01 00:00:00
abstract::Positive newborn screening (NBS) results cause significant parental distress, but little is known about how parents find out about children's screening results and what they are told. This qualitative, exploratory study reports on parents' perceptions of the initial communication of NBS results. Participants included ...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922812446011
更新日期:2012-08-01 00:00:00
abstract::The Internet, an extensive network of interlinked computers storing immense quantities of information, is growing exponentially. The author was interested in discovering what sources of information exist on this network that might be of importance to the pediatric health care provider. To accomplish this aim, a litera...
journal_title:Clinical pediatrics
pub_type: 历史文章,杂志文章,评审
doi:10.1177/000992289603500501
更新日期:1996-05-01 00:00:00
abstract::Premature delivery is common in pregnancies complicated by maternal diabetes. However, the outcome of very-low-birth-weight infants (VLBWI) born to mothers with diabetes is not known. Employing a matched double-cohort design, we investigated the influence of maternal diabetes on the outcome of VLBWI born in Winnipeg f...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992280204100705
更新日期:2002-09-01 00:00:00
abstract::A case of an 11-year-old girl with autoimmune hypothyroidism and a positive sweat test is presented. When the hypothyroidism was corrected the sweat test reverted to normal. ...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288902801110
更新日期:1989-11-01 00:00:00
abstract::There is a paucity of data on the impact of mobile technology on physician-led education in the pediatric population. We performed a prospective pilot study in a pediatric diabetes clinic to evaluate the impact of tablet-based patient education. A total of 106 patients and caregivers completed postclinic surveys, 64 (...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922818766621
更新日期:2018-09-01 00:00:00
abstract::Family health history (FHH) can identify families at increased risk for disease. Purpose. To learn mothers' (1) perceptions of the benefits of FHH and (2) willingness to complete a FHH tool, My Family Health Portrait (MFHP). Methods. Qualitative in-depth interviews were conducted with mothers recruited through Cincinn...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922812467531
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Ethanol lock therapy (ELT) with systemic antimicrobial therapy is a promising therapy for catheter-related infection (CRI). The impact of ELT timing on treatment efficacy and costs is unknown. PROCEDURES:A prospective study was conducted in the Hematology/Oncology Unit at the Children's Hospital of Michigan...
journal_title:Clinical pediatrics
pub_type: 杂志文章,随机对照试验
doi:10.1177/0009922817717327
更新日期:2018-03-01 00:00:00
abstract::Topical application of ocular drugs may cause serious adverse ocular or systemic side effects. Children are at greater risk for systemic side effects because ocular dosing is not weight-adjusted, and infants are especially vulnerable as a consequence of the inability to efficiently metabolize the drug, and/or an immat...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992280404300114
更新日期:2004-01-01 00:00:00
abstract::This study was undertaken to evaluate trends and toxicity of pediatric methylphenidate exposures; 1993 to 1999 national poison center data were analyzed. There were 12,917 exposures, increasing from 927 in 1993 to 2,445 in 1999. The majority of children during the entire study period experienced no effect (60.3%) or m...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992280304200210
更新日期:2003-03-01 00:00:00
abstract::Childhood thyrotoxicosis is not an uncommon illness, but there is a considerable relapse rate in these patients on medical treatment. An attempt is made in this study to identify those markers that are predictive of a tendency to relapse in childhood thyrotoxicosis. A total of 32 patients with Graves disease were incl...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288702600306
更新日期:1987-03-01 00:00:00
abstract::Between 1979 and 1981, 22 children with osteomyelitis and/or septic arthritis were hospitalized at Soroka University Hospital. Streptococcus pneumoniae was cultured in five of the children, four of whom were under 2 years of age. In four other children under 2 years of age, Haemophilus influenzae was cultured. Staphyl...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288502400305
更新日期:1985-03-01 00:00:00
abstract::Attendance at well-child visits (WCVs) is a sine qua non of preventive care. We hypothesized that Reach Out and Read (ROR) would be associated with better WCV attendance. Parents of children 76 to 72 months at 8 clinics who did not yet have ROR reported how many WCVs their child had attended in the previous year; sepa...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922818822975
更新日期:2019-01-07 00:00:00
abstract:BACKGROUND:There are some studies demonstrating the effectiveness of the provision of written asthma action plans in improving asthma outcomes. There exist little data on the ability of parents to use these plans to make asthma care decisions. OBJECTIVE:To assess the associations between parental health literacy (HL),...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/0009922815587089
更新日期:2016-02-01 00:00:00
abstract::Serum IgG, IgM, and IgA were measured in 316 infants younger than 12 months of age. Information including the child's race, sex, age, and past history of infections or atopy was collected. Birth records were also reviewed to ascertain the child's birthweight and gestational age. Serum IgG levels were higher in black i...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288502400204
更新日期:1985-02-01 00:00:00
abstract::The differential leukocyte count was studied within the first 24 hours of life in 115 infants of diabetic mothers (IDMs) appropriate for gestational age (AGA), 16 IDMs large for gestational age (LGA), 104 infants of non-diabetic mothers (INM's) AGA, and 22 INMs-LGA. A significant "shift to the left" was found in IDM's...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288602500806
更新日期:1986-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy and feasibility of a multifaceted, community-based weight intervention program for children using exergaming technology (activity-promoting video gaming). DESIGN AND METHODS:This is a prospective observational pilot study. Forty-eight children, between the ages of 8 and 16 years, who...
journal_title:Clinical pediatrics
pub_type: 临床试验,杂志文章
doi:10.1177/0009922811429480
更新日期:2012-04-01 00:00:00
abstract::Mammalian bites account for a large number of emergency department and doctor's visits. Children are the victims in more than half of reported cases. Dogs account for the majority of wounds, and almost all fatalities in these cases are due to dog bites. Human bites and cat bites account for the majority of infected wo...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992289002900506
更新日期:1990-05-01 00:00:00
abstract::Three infants who presented with premature rupture of membranes and neonatal pneumonia caused by organisms belonging to members of the B. fragilis group are reported. In all 3 instances, the organisms were recovered from tracheal aspirates and in 2 from blood cultures as well. Two of the newborns, treated with ampicil...
journal_title:Clinical pediatrics
pub_type: 杂志文章
doi:10.1177/000992288001900807
更新日期:1980-08-01 00:00:00