Abstract:
BACKGROUND:Enzyme-linked immunosorbent assay (ELISA) is the most popular method used by blood banks in China to screen for antibodies to Treponema pallidum (TP). However, this method produces a high percentage of false-positive reactions.The current study aimed to propose a more effective procedure for screening anti-TP antibodies in blood donors. METHODS:A total of 1009 serum samples showing repeat reactivity in routine blood bank procedures were tested with a TP particle agglutination assay (TPPA) and 3 commercially available ELISA tests. Samples showing reactivity with any of these methods were further tested by Western blot (WB) and ELISA immunoglobulin G (IgG)and immunoglobulin M (IgM) anti-TP assays. RESULTS:Of the 1009 samples tested, 170 were identified as reactive with at least one of our methods. In these 170 samples, excluding borderline cases, the agreement of the WB method with TPPA, Murex, BGI, and InTec ELISA tests was 76.0%, 77.8%, 78.4%, and 80.1%, respectively. With the WB results as the reference, the sensitivity of TPPA, Murex, BGI, and InTec ELISA tests was 91.5%, 95.4%, 99.2%, and 98.5%, respectively. TpN17 and TpN15 are the most important antigens in the antibody response of IgM and IgG, respectively. The WB IgM and IgG results suggest that the seroprevalence of anti-TP in blood donors in China is 0.49% (130/26,702). CONCLUSIONS:A combination of routine ELISA methods and WB IgM and IgG may be a valid procedure for the confirmatory testing of anti-TP antibodies and would be beneficial for screening blood donors.
journal_name
Sex Transm Disjournal_title
Sexually transmitted diseasesauthors
Wang LN,Li JMdoi
10.1097/OLQ.0b013e31819db594subject
Has Abstractpub_date
2009-07-01 00:00:00pages
413-6issue
7eissn
0148-5717issn
1537-4521journal_volume
36pub_type
杂志文章abstract:BACKGROUND:Routine chlamydia screening is not readily available at all college campuses. OBJECTIVES:: To assess the prevalence of Chlamydia trachomatis among asymptomatic college students and to compare chlamydia positivity by selected demographic variables. METHODS:Analysis of demographical data collected on 789 stu...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0b013e3181676697
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE/GOAL:The objective of this study was to examine sildenafil (Viagra) use and its relationship to sexual risk behavior for HIV and sexually transmitted diseases among men who have sex with men (MSM). STUDY:A population-based telephone sample of MSM in San Francisco was interviewed about sexual behavior, substa...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/01.olq.0000175294.76494.77
更新日期:2005-09-01 00:00:00
abstract::In every society, congenital syphilis (CS) has significant medical, economic, societal and emotional burdens; these are poorly characterized but high. Inexplicably, the elimination of CS has failed to attract international attention. Yet, the cornerstones of programs to do this universally are in place, the recent dev...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章,评审
doi:10.1097/01.olq.0000261456.09797.1b
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:The integration of HPV-16 DNA into the host genome is considered an important event in the progression of premalignant cervical lesions to cervical cancer. The aim of our study was to assess the prevalence of HPV-16 integration in anal cytologic specimens of HIV-1 infected men and its association with risk f...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0b013e3181c9c23f
更新日期:2010-05-01 00:00:00
abstract::Forty heterosexual male patients with therapy resistant penile warts of long duration (mean 12.9 months) were treated with carbon dioxide laser, immediately followed by topical application of 0.5% idoxuridine cream twice daily for 14 days. In case of incomplete or no response to the initial treatment, the treatment pr...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/00007435-199007000-00003
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:Herpes simplex virus type 2 (HSV-2) is an important cause of genital ulcers and can increase the risk for human immunodeficiency virus type 1 (HIV-1) transmission. Our objective was to determine the incidence and correlates of HSV-2 infection in HIV-1-seronegative Kenyan men reporting high-risk sexual behavi...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0b013e31821a6225
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVES:Screening women for Chlamydia trachomatis (CT) infection using selective screening criteria has been operational in the northwestern United States (Region X) since 1988. Changes in the field, including declines in CT prevalence, introduction of sensitive laboratory tests, and budgetary pressures necessitate ...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/01.olq.0000124613.85111.6b
更新日期:2004-05-01 00:00:00
abstract::Increasing evidence suggests that human papillomavirus (HPV) infection of the female anogenital tract is multifocal. Less is known of the distribution of HPVs in men. To investigate this, a prospective study was conducted of 116 men consecutively attending a clinic for ablative treatment of anogenital warts. Wart tiss...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/00007435-199301000-00005
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Different models of sexually transmitted infections (STIs) can yield substantially different conclusions about STI epidemiology, and it is important to understand how and why models differ. Frequency-dependent models make the simplifying assumption that STI incidence is proportional to STI prevalence in the ...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0000000000000412
更新日期:2016-03-01 00:00:00
abstract::In developed nations, Chlamydia trachomatis is the most common sexually transmitted pathogen. To determine whether prior disease affects the probability of subsequent chlamydial infection, we took culture specimens from 2,546 men and 1,998 women attending a sexually transmitted diseases clinic. The men had nongonococc...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/00007435-198707000-00008
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:Obstetric and gynecologic complications associated with bacterial vaginosis and trichomoniasis have heightened awareness of these conditions, and thus metronidazole's role in obstetric and gynecologic practice continues to increase. GOAL OF THIS STUDY:To review the clinical indications for metronidazole in ...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章,评审
doi:
更新日期:1995-11-01 00:00:00
abstract::Selected results from a recent study on sexual behavior in the United States are presented, focusing on the association between sexual behavior, disease prevention, and STD outcome. The data are from national household samples of adult men and women. The results show that a sizeable proportion of the U.S. adult popula...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::Given the many options available, selecting an HIV test for a particular clinical or research setting can be daunting. Making an informed decision requires an assessment of the likelihood of acute infection in the test population and an understanding of key aspects of the tests themselves. The ability of individual te...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章,评审
doi:10.1097/OLQ.0000000000000719
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the outcomes of a partner referral program at the sexually transmitted disease (STD) clinic in Amsterdam that encourages gonorrhea and chlamydia patients to refer their own partners to medical examination. STUDY DESIGN:355 patients with a confirmed infection cooperated in partner referral and gav...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/00007435-199707000-00005
更新日期:1997-07-01 00:00:00
abstract:OBJECTIVES:To examine the epidemiology of multiple infections in teenagers and their contacts. GOAL:To demonstrate a network approach to the prevalence of STDs/HIV. STUDY DESIGN:A community-based network study of ethnographically representative adolescents. RESULTS:Of the 512 interviewees, 90% of the boys and 80% of...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/01.olq.0000258132.06764.a1
更新日期:2007-08-01 00:00:00
abstract::One hundred sixteen patients (92 men and 24 women) with suspected uncomplicated gonorrhea were randomized in a double-blind manner to receive intramuscular treatment with 1.0 g of cefpimizole, 1.0 g of cefotaxime, or 4.8 x 10(6) units of aqueous procaine penicillin G (APPG) with 1 g of oral probenecid. Seventeen perce...
journal_title:Sexually transmitted diseases
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007435-198804000-00001
更新日期:1988-04-01 00:00:00
abstract:BACKGROUND:To prevent congenital syphilis, the Centers for Disease Control and Prevention and professional organizations recommend universal prenatal syphilis screening. State-level or larger-scale evaluations of adherence to these guidelines have relied on administrative data. We measured prenatal syphilis screening r...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0b013e31815fa5bb
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:The female condom is a viable option for women to protect themselves from HIV infection and other sexually transmitted diseases. GOAL:The goal was to examine the level of female condom use and factors associated with frequency of use among US women living in San Francisco and Oakland, California. STUDY DES...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/00007435-200301000-00018
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:The use of sexual network analysis has the potential to further our understanding of sexually tranmitted disease (STD) epidemics and contribute to the development of more effective targeted control strategies. GOAL:To use sexual network analysis to study transmission patterns of chlamydia and gonorrhea in M...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/00007435-200101000-00005
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Few men are tested for syphilis or human immunodeficiency virus (HIV) during their partner's pregnancy, a high-risk period for HIV and syphilis transmission. Offering home-based rapid testing of syphilis to couples during pregnancy can support prevention efforts to reduce transmission of sexually transmitted...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章,随机对照试验
doi:10.1097/OLQ.0000000000000649
更新日期:2017-09-01 00:00:00
abstract::Performance characteristics of the Cobas 4800 CT/NG assay were estimated for vaginal swab samples from more than 4000 women. Vaginal samples identified 92.9% and 98.5% of Chlamydia and Gonorrhea infections, respectively, which was more than any other single specimen type (endocervical swabs or urine) regardless of col...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1097/OLQ.0b013e3182717833
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Adherence to recommended laboratory testing practices is crucial for sexually transmitted infection (STI) prevention and control. The objective of this paper is to compare Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) testing practices of US clinical laboratories in 2013 prior to the updated 2014...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0000000000001299
更新日期:2020-09-18 00:00:00
abstract::Using data from the 2006-2010 National Survey of Family Growth, we found that lifetime number of vaginal sex partners is positively associated with the number of sex partners in the past 12 months and negatively associated with age at first vaginal intercourse among 15- to 44-year-old male and female respondents. ...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0000000000000132
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:The prevalence of human papillomavirus (HPV) infection and the distribution of different HPV genotypes vary greatly within different geographical and ethnic populations, especially in Asia. The HPV infection data based on regional population are extremely important for researchers to develop new efficient HP...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0000000000000201
更新日期:2014-12-01 00:00:00
abstract::A new variant of Chlamydia trachomatis (nvCT) was discovered in Sweden in 2006, and it could not be detected by diagnostic systems from Abbott and Roche, whereas the third system used, from Becton Dickinson (BD), detects nvCT. We analyzed 3648 samples from 2 counties that used Roche and 2 counties that used BD methods...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0b013e3182593be7
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:The association between area-based social factors and sexually transmitted diseases has been demonstrated in numerous studies. Such associations have not previously been explored for their potential to quantify likelihood of higher transmission of gonorrhea in small geographic areas. METHODS:Aggregate censu...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章,多中心研究
doi:10.1097/OLQ.0000000000000195
更新日期:2014-11-01 00:00:00
abstract::Partner notification for HIV and other sexually transmitted infections is acceptable and feasible among female sex workers attending sexually transmitted infection clinics in Guatemala, especially for regular partners. Intention to refer the sexual partner was best predicted by attitude followed by social norms and ba...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0b013e31824f8b1b
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Methods of HIV and STD prevention, which can be controlled by the receptive partner, are a high priority for research and development. Studies on the safety of Nonoxynol-9 (N-9) on the vaginal mucosa have yielded conflicting results. No Phase I study has evaluated the effect of N-9 on the rect...
journal_title:Sexually transmitted diseases
pub_type: 临床试验,杂志文章
doi:10.1097/00007435-199911000-00005
更新日期:1999-11-01 00:00:00
abstract:OBJECTIVES:To evaluate the incidence of early syphilis based on time from initiation of highly active antiretroviral therapy (HAART) in human immunodeficiency virus (HIV)-infected patients. STUDY DESIGN:Five hundred thirty-nine HIV-positive patients undergoing HAART were followed up to 4 years to identify early (prima...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0b013e31815b0148
更新日期:2008-03-01 00:00:00
abstract::We believe this to be the first report of coexisting polymerase chain reaction (PCR)-confirmed primary genital and ocular syphilis in a patient with penicillin allergy treated with doxycycline. The case examines the use of nonpenicillin and corticosteroid treatment of ocular syphilis and highlights the risk of eye inv...
journal_title:Sexually transmitted diseases
pub_type: 杂志文章
doi:10.1097/OLQ.0000000000000900
更新日期:2018-12-01 00:00:00