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CLCNKB-T481S and essential hypertension in a Ghanaian population.

Abstract：

OBJECTIVE:Prior to the discovery of chloride channel Kb with a variant threonine change to serine at position 481 (CLCNKB-T481S) there were no variants or clinical disorders associated with gain-of-function defects in thick ascending limb of the kidney channels or transporters. CLCNKB-T481S is a novel gain-of-function variant that has been associated with essential hypertension. This finding has not been replicated until our current study. In this study, we re-examined CLCNKB-T481S using a large homogenous population from Ghana, and coupled genetic analyses with the functional characterization of this polymorphism using a mammalian expression system. METHODS:We genotyped CLCNKB-T481S in four ethnically defined control populations and a homogenous cohort of normotensive and hypertensive Ghanaians. Functional analysis was performed by whole-cell patch-clamp recording of tsA201 cells (a cell line derived from the human renal cell line, HEK-293) transiently transfected with ClC-Kb and barttin. RESULTS:CLCNKB-T481S was found more commonly in the African and Caucasian-Americans when compared with the Asian and Hispanic American populations, having minor allele frequencies of 0.20, 0.15, 0.06 and 0.01 respectively. Additionally, CLCNKB-T481S was significantly associated with hypertension in Ghanaian males. In stratified logistic regression analysis with Ghanaian males, we observed a significant odds ratio of 3.29 (1.17-9.20 95% confidence interval, P=0.024) in the recessive model (TT versus AT&TT). Unlike previous results obtained in Xenopus oocytes, coexpression of CLCNKB-T481S with the obligatory accessory subunit barttin in tsA201 cells did not generate larger currents than coexpression of the wild-type allele. CONCLUSIONS:We conclude that CLCNKB-T481S is associated with essential hypertension in males within the Ghanaian population; however, further studies are needed to understand its sex and ethnic segregation as well as to identify cellular factors that account for the divergent functional expression of ClC-Kb-T481S plus barttin in Xenopus oocytes and mammalian cells.

journal_name

J Hypertens

journal_title

Journal of hypertension

authors

Sile S,Velez DR,Gillani NB,Narsia T,Moore JH,George AL Jr,Vanoye CG,Williams SM

doi

10.1097/hjh.0b013e3283140c9e

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

298-304

issue

eissn

0263-6352

issn

1473-5598

journal_volume

pub_type

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CLCNKB-T481S and essential hypertension in a Ghanaian population.