Human erythrocytes and molecular models of cell membranes are affected in vitro by Balbisia peduncularis (Amancay) extracts.


:Balbisia peduncularis, also known as "Amancay", is a plant of the Ledocarpaceae family that can be found in the Atacama Desert in northern Chile. Infusions of the plant have long being used in traditional herbal medicine. Its chemical composition indicates the presence of flavonoids, which have antioxidant properties. Aqueous extracts from its stems were prepared to induce their interaction with human erythrocytes and their membrane models in order to elucidate whether this rare and unstudied plant produced perturbations to cell membranes. Scanning electron microscopy (SEM) of intact human red blood cells showed that the extract changed the normal erythrocytes morphology as a function of its concentration, first inducing echinocytes, and then stomatocytes and spherocytes. According to the bilayer couple hypothesis, the shape changes indicated that the flavonoids were first located in the outer monolayer of the erythrocyte membrane, and at the highest assayed concentration in both monolayers. The results obtained by fluorescence spectroscopy measurements of isolated unsealed human erythrocytes (IUM), of unilamellar vesicles (LUV) of dimyristoylphosphatidylcholine (DMPC), and by X-ray diffraction of DMPC and dimyristoylphosphatidylethanolamine (DMPE) multilayers, confirmed this conclusion. In fact, they showed that the plant aqueous extract molecules were located in both the hydrophilic polar head and in the hydrophobic acyl chain regions of the lipid bilayers. As a consequence, perturbations of the phospholipid bilayer packing arrangement were produced.


Chem Biol Interact


Suwalsky M,Oyarce K,Avello M,Villena F,Sotomayor CP




Has Abstract


2009-05-15 00:00:00














  • 20(S)-protopanaxadiol (PPD) alleviates scopolamine-induced memory impairment via regulation of cholinergic and antioxidant systems, and expression of Egr-1, c-Fos and c-Jun in mice.

    abstract::20(S)-protopanaxadiol (PPD) possesses various biological properties, including anti-inflammatory, antitumor and anti-fatigue properties. Recent studies found that PPD functioned as a neurotrophic agent to ameliorate the sensory deficit caused by glutamate-induced excitotoxicity through its antioxidant effects and exhi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Lu C,Dong L,Lv J,Wang Y,Fan B,Wang F,Liu X

    更新日期:2018-01-05 00:00:00

  • Redox cycling of polycyclic aromatic hydrocarbon o-quinones: metal ion-catalyzed oxidation of catechols bypasses inhibition by superoxide dismutase.

    abstract::Several two-electron quinone reductases catalyze the redox cycling of polycyclic aromatic hydrocarbon (PAH) o-quinones. When the carbonyl reductase of human placenta catalyzes the cycling of 9,10-phenanthrenequinone in aqueous phosphate buffer, reactive oxygen species are produced. Superoxide dismutase (SOD) inhibits ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Jarabak R,Harvey RG,Jarabak J

    更新日期:1998-10-02 00:00:00

  • An albumin-butyrylcholinesterase for cocaine toxicity and addiction: catalytic and pharmacokinetic properties.

    abstract::Butyrylcholinesterase (BChE, EC is important in human cocaine metabolism despite its limited ability to hydrolyze this drug. Efforts to improve the catalytic efficiency of this enzyme have led to a quadruple mutant cocaine hydrolase, "CocH", that in animal models of addiction appears promising for treatment o...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Gao Y,LaFleur D,Shah R,Zhao Q,Singh M,Brimijoin S

    更新日期:2008-09-25 00:00:00

  • Unwinding of supercoiled Col E1-DNA after covalent binding of the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative.

    abstract::The unwinding of superhelical Col E1-DNA was studied by means of gel electrophoresis and electron microscopy after covalent binding of N-acetoxy-N-2-[14C]acetylaminofluorene (N-Aco-[14C]AAF) and its 7-iodo derivative (N-Aco-[14C]AAIF). Studies with both compounds indicated that complete unwinding of the supercoiled DN...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Lang MC,Freund AM,de Murcia G,Fuchs RP,Daune MP

    更新日期:1979-12-01 00:00:00

  • Transcriptional profiling suggests that Nevirapine and Ritonavir cause drug induced liver injury through distinct mechanisms in primary human hepatocytes.

    abstract::Drug induced liver injury (DILI), a major cause of pre- and post-approval failure, is challenging to predict pre-clinically due to varied underlying direct and indirect mechanisms. Nevirapine, a non-nucleoside reverse transcriptase inhibitor (NNRTI) and Ritonavir, a protease inhibitor, are antiviral drugs that cause c...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Terelius Y,Figler RA,Marukian S,Collado MS,Lawson MJ,Mackey AJ,Manka D,Qualls CW Jr,Blackman BR,Wamhoff BR,Dash A

    更新日期:2016-08-05 00:00:00

  • Biological and toxicological consequences of quinone methide formation.

    abstract::Quinone methides are a class of reactive, electrophilic compounds which are capable of alkylating cellular macromolecules. They are formed during xenobiotic biotransformation reactions and are hypothesized to mediate the toxicity of a large number of quinone antitumor drugs as well as several alkylphenols. In addition...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审


    authors: Thompson DC,Thompson JA,Sugumaran M,Moldéus P

    更新日期:1993-02-01 00:00:00

  • Achieving resistance specificity in prostate cancer.

    abstract::Prostate (CaP) cancer is the second-leading cause of cancer-related mortality in men in Western societies. Androgen receptor (AR) signaling is a critical survival pathway for prostate cancer cells, and androgen-deprivation therapy (ADT) remains the principal treatment for patients with locally advanced and metastatic ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审


    authors: Wadhwa B,Dumbre R

    更新日期:2016-12-25 00:00:00

  • Novel ferrocenyl pyrazoles inhibit breast cancer cell viability via induction of apoptosis and inhibition of PI3K/Akt and ERK1/2 signaling.

    abstract::Despite the advances in early detection and targeted therapies, chemotherapy is still of vital importance in breast cancer treatment. However, development of drug resistance and serious side effects limits their usage. Thus, there is an urgent need for safer and more effective agents against breast cancer. We have pre...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Atmaca H,Özkan AN,Zora M

    更新日期:2017-02-01 00:00:00

  • Characterization of multiple Chinese hamster carbonyl reductases.

    abstract::Carbonyl reductase (CR) is an enzyme which can catalyze the oxidoreduction of various carbonyl compounds in the presence of NAD(P)H. With the PCR method, using primers carrying the conserved nucleotide sequence among mammalian CRs, we isolated three different cDNAs (CHCR1, CHCR2 and CHCR3) which encode a unique carbon...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Terada T,Sugihara Y,Nakamura K,Sato R,Sakuma S,Fujimoto Y,Fujita T,Inazu N,Maeda M

    更新日期:2001-01-30 00:00:00

  • A comprehensive review on hepatoprotective and nephroprotective activities of chrysin against various drugs and toxic agents.

    abstract::Chrysin belongs to the flavonoids and has been used as traditional medicine from ancient and has been reported to exhibit a wide range of pharmacological properties. The biochemical and molecular mechanisms involved in the hepato- and nephroprotective activities of chrysin were discussed in this review. Chrysin exhibi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审


    authors: Pingili RB,Pawar AK,Challa SR,Kodali T,Koppula S,Toleti V

    更新日期:2019-08-01 00:00:00

  • iTRAQ-based quantitative proteomics discovering potential serum biomarkers in locoweed poisoned rabbits.

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    journal_title:Chemico-biological interactions

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    authors: Jiao J,Wang S,Zhang R,Ma Z,Du G,Chen X,Tao D,Zhao J

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  • In vitro and in vivo anticancer activity of posterior salivary gland toxin from the cuttlefish Sepia pharaonis, Ehrenberg (1831).

    abstract::Posterior salivary gland (PSG) toxins are high molecular weight toxins secreted by cephalopods and gastropods which possess immense potentials in biomedical applications. In the present study, the biomedical potentials of the PSG toxin from the cuttlefish, S. pharaonis was determined in vitro and in vivo. The cytostat...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Karthik R,Manigandan V,Ebenezar KK,Vijayashree R,Saravanan R

    更新日期:2017-06-25 00:00:00

  • The in vitro metabolites of 2,4,6-trichlorophenol and their DNA strand breaking properties.

    abstract::The carcinogenic compound 2,4,6-trichlorophenol (2,4,6-TCP) was incubated with rat liver S-9 fraction. Three metabolites were identified: 2,6-dichloro-1,4-hydroquinone (DHQ), and two isomers of hydroxypentachlorodiphenyl ether (OH-Cl5-DPE). The latter are probably products of microsomal .OH radical attack on the trich...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Juhl U,Blum K,Witte I

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  • Salidroside attenuates colistin-induced neurotoxicity in RSC96 Schwann cells through PI3K/Akt pathway.

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    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Lu Z,Jiang G,Chen Y,Wang J,Muhammad I,Zhang L,Wang R,Liu F,Li R,Qian F,Li J

    更新日期:2017-06-01 00:00:00

  • Sodium butyrate reduces insulin-resistance, fat accumulation and dyslipidemia in type-2 diabetic rat: A comparative study with metformin.

    abstract::Recent evidences highlighted that histone deacetylases (HDACs) can deacetylate the histone, various transcription factors and regulatory proteins, which directly or indirectly affect glucose metabolism. The present study aimed to evaluate the comparative effects of sodium butyrate (NaB) and metformin on the glucose ho...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Khan S,Jena G

    更新日期:2016-07-25 00:00:00

  • Mechanistic evaluation of gastroprotective effects of Kangfuxin on ethanol-induced gastric ulcer in mice.

    abstract::This study was designed to evaluate the gastroprotective effect of Kangfuxin (KFX), a Chinese patent medicine constituent isolated from American cockroach, on ethanol-induced gastric ulcer in mice and to elucidate the potential mechanisms of the effect involved. According to the results, mice treated with alcohol appe...

    journal_title:Chemico-biological interactions

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    authors: Shen Y,Sun J,Niu C,Yu D,Chen Z,Cong W,Geng F

    更新日期:2017-08-01 00:00:00

  • The relationship between the effects of drugs on bilayer stability and on protein kinase C activity.

    abstract::A variety of substances of diverse structure have been shown to affect the activity of protein kinase C. Many of the agents which affect protein kinase C activity also markedly shift the bilayer to hexagonal phase transition temperature of phosphatidylethanolamines. Although one of the more potent activators of protei...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Epand RM

    更新日期:1987-01-01 00:00:00

  • Mangiferin attenuates blast-induced traumatic brain injury via inhibiting NLRP3 inflammasome.

    abstract::There is growing evidence that Mangiferin possess therapeutic benefit during neuroinflammation on various brain injury models due to its anti-inflammatory properties. It is reported that inflammatory plays a crucial role in the pathogenesis of secondary injury induced by the blast-induced traumatic brain injury (bTBI)...

    journal_title:Chemico-biological interactions

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    authors: Fan K,Ma J,Xiao W,Chen J,Wu J,Ren J,Hou J,Hu Y,Gu J,Yu B

    更新日期:2017-06-01 00:00:00

  • Correlation between the substrate structure and the rate of acetylcholinesterase hydrolysis modeled with the combined quantum mechanical/molecular mechanical studies.

    abstract::The combined quantum mechanical-molecular mechanical (QM/MM) based computational scheme for modeling the structure-reaction rate correlations was elaborated for the hydrolysis of the set of neutral esters in the active site of acetylcholinesterase (AChE). The energy barriers of hydrolysis were estimated on the basis o...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Lushchekina SV,Nemukhin AV,Morozov DI,Varfolomeev SD

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  • Carbamylation and oxidation of proteins lead to apoptotic death of lymphocytes.

    abstract::The apoptotic/necrotic changes in isolated human peripheral blood mononuclear cells (MNCs) subjected to hydrogen peroxide (H2O2), cyanate (NaOCN) and their combination were examined. The mitochondrial potential (ΔΨm), the activities of caspases (-2, -3, -6, -8 and -9) and the level of carbonyls and amino groups in pro...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Pieniazek A,Gwozdzinski K

    更新日期:2017-05-25 00:00:00

  • Mangiferin attenuates DSS colitis in mice: Molecular docking and in vivo approach.

    abstract::Inflammation, oxidative stress and altered mucosal barrier permeability are potential etiopathological or triggering factors for inflammatory bowel disease (IBD). In this study, the therapeutic potential of Mangiferin was investigated in vivo in mouse model of colitis and also attempts were made to understand mechanis...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Somani S,Zambad S,Modi K

    更新日期:2016-06-25 00:00:00

  • The effects of indole-3-acetic acid on human and horse serum butyrylcholinesterase.

    abstract::Butyrylcholinesterase (BChE) constitutes the first line defense in the serum of higher organisms and is a marker for toxic exposure. Indole-3-acetic acid (IAA) is a major plant growth hormone of the auxin class, affecting cell enlargement, and differentiation. As a result of the industrial usage, this agrochemical is ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Bodur E,Cokugras AN

    更新日期:2005-12-15 00:00:00

  • Inhibition of stage-specific DNA synthesis in rat spermatogenic cells by polycyclic aromatic hydrocarbons.

    abstract::Changes in the rate of DNA synthesis in spermatogenic cells after treatment of segments of rat seminiferous tubule at defined stages of epithelial cycle with benzo[a]pyrene (BP) or 7,12-methylbenz[a]anthracene (DMBA) were studied. The incorporation of labeled thymidine into DNA was used as a measure of the rate of DNA...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Georgellis A,Parvinen M,Rydström J

    更新日期:1989-01-01 00:00:00

  • Caffeine does not bind covalently to liver microsomes from different animal species and to proteins and DNA from perfused rat liver.

    abstract::Caffeine was found not to bind covalently to liver microsomal proteins from mice, rats and rabbits. Microsomes metabolized caffeine only to a limited extent, the highest rate (about 2% of the substrate concentration) being obtained with rabbit microsomal preparations. The rat liver perfusion technique represents a goo...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Szczawinska K,Ginelli E,Bartosek I,Gambazza C,Pantarotto C

    更新日期:1981-03-15 00:00:00

  • The microsomal metabolism of pentachlorophenol and its covalent binding to protein and DNA.

    abstract::The microsomal metabolism of pentachlorophenol (PCP) was investigated, with special attention to the conversion dependent covalent binding to protein and DNA. The two metabolites detected were tetrachloro-1,2- and tetrachloro-1,4-hydroquinone. Microsomes from isosafrole (ISF)-induced rats were by far the most effectiv...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: van Ommen B,Adang A,Müller F,van Bladeren PJ

    更新日期:1986-10-15 00:00:00

  • Apparent stimulation of dibenzo[a,e]fluoranthene in vitro metabolism in the presence of norharman.

    abstract::The effects of norharman (NH) on the metabolism of dibenzo[a,e]-fluoranthene (DBF) and on its fixation on DNA, RNA and proteins have been studied in vitro by incubation with S-9 and microsomes from rats and mice. NH causes a decrease of the activity of microsome monooxygenases proportionally to its concentration but h...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Perin-Roussel O,Ekert B,Zajdela F

    更新日期:1981-10-01 00:00:00

  • Whole body and tissue imaging of the butyrylcholinesterase knockout mouse injected with near infrared dye labeled butyrylcholinesterase.

    abstract::Butyrylcholinesterase (BChE) has proven to be an effective bioscavenger against nerve agents and organophosphates. Phase I safety trials of human BChE are currently being conducted and large-scale production of recombinant BChE is underway. Information on the real-time distribution of BChE from the injection site has ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Duysen EG,Lockridge O

    更新日期:2008-09-25 00:00:00

  • Methylation of DNA in rat liver and intestine by dimethylnitrosamine and N-methylnitrosourea.

    abstract::A chromatographic procedure for improved separation of deoxyribonucleosides and methylated deoxyribonucleosides is described. DNA was isolated from liver and small intestine of rats treated with [14C]dimethylnitrosamine ([14C]DMN) or N-[3H]methyl-N-nitrosourea ([3H]MNU), and the purified DNA was hydrolyzed enzymatical...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Daoud AH,Irving CC

    更新日期:1977-02-01 00:00:00

  • Chromatographic resolution of amino acid adducts of aliphatic halides.

    abstract::Numerous xenobiotics are known to be bioactivated and to covalently and to proteins, but the resulting amino acid adducts (AAAs) are unknown. In this study the AAAs of twelve 14C-labeled aliphatic halides were examined after formation in an in vitro microsomal system. After exhaustive solvent extraction of the precipi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Maiorino RM,Gandolfi AJ,Brendel K,Mac Donald JR,Sipes IG

    更新日期:1982-01-01 00:00:00

  • Arsenic, cadmium, mercury and nickel stimulate cell growth via NADPH oxidase activation.

    abstract::Exposure to metals and metalloids including arsenic, cadmium, mercury, and nickel has been a worldwide health problem for several decades. The aim of this study was to learn how metal-induced oxidative stress triggers cell proliferation, a process of great significance for cancer. NADPH oxidase (NOX) activity and cell...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Mohammadi-Bardbori A,Rannug A

    更新日期:2014-12-05 00:00:00