当前位置： SCI文献检索 > CHEMICO-BIOLOGICAL INTERACTIONS期刊下所有文献 > Monoclonal antibodies to human butyrylcholinesterase reactive with butyrylcholinesterase in animal plasma.

Monoclonal antibodies to human butyrylcholinesterase reactive with butyrylcholinesterase in animal plasma.

Abstract：

:Five mouse anti-human butyrylcholinesterase (BChE) monoclonal antibodies bind tightly to native human BChE with nanomolar dissociation constants. Pairing analysis in the Octet system identified the monoclonal antibodies that bind to overlapping and independent epitopes on human BChE. The nucleotide and amino acid sequences of 4 monoclonal antibodies are deposited in GenBank. Our goal was to determine which of the 5 monoclonal antibodies recognize BChE in the plasma of animals. Binding of monoclonal antibodies 11D8, B2 18-5, B2 12-1, mAb2 and 3E8 to BChE in animal plasma was measured using antibody immobilized on Pansorbin cells and on Dynabeads Protein G. A third method visualized binding by the shift of BChE activity bands on nondenaturing gels stained for BChE activity. Gels were counterstained for carboxylesterase activity. The three methods agreed that B2 18-5 and mAb2 have broad species specificity, but the other monoclonal antibodies interacted only with human BChE, the exception being 3E8, which also bound chicken BChE. B2 18-5 and mAb2 recognized BChE in human, rhesus monkey, horse, cat, and tiger plasma. A weak response was found with rabbit BChE. Monoclonal mAb2, but not B2 18-5, bound pig and bovine BChE. Gels stained for carboxylesterase activity confirmed that plasma from humans, monkey, pig, chicken, and cow does not contain carboxylesterase, but plasma from horse, cat, tiger, rabbit, guinea pig, mouse, and rat has carboxylesterase. Rabbit plasma carboxylesterase hydrolyzes butyrylthiocholine. In conclusion monoclonal antibodies B2 18-5 and mAb2 can be used to immuno extract BChE from the plasma of humans, monkey and other animals.

journal_name

Chem Biol Interact

journal_title

Chemico-biological interactions

authors

Peng H,Brimijoin S,Hrabovska A,Krejci E,Blake TA,Johnson RC,Masson P,Lockridge O

doi

10.1016/j.cbi.2015.11.011

subject

Has Abstract

pub_date

2016-01-05 00:00:00

pages

82-90

eissn

0009-2797

issn

1872-7786

pii

S0009-2797(15)30113-7

journal_volume

243

pub_type

杂志文章

相关文献

CHEMICO-BIOLOGICAL INTERACTIONS文献大全
  • Anticancer activity and radiosensitization effect of methyleneisoxazolidin-5-ones in hepatocellular carcinoma HepG2 cells.

    abstract：:Parthenolide (PTL), a well-known sesquiterpene lactone of natural origin with α,β-unsaturated carbonyl structure, has proven to show promising anti-cancer properties. In this report, anti-proliferative potential of two synthetic methyleneisoxazolidin-5-ones, MZ-6 and MZ-14, with the same structural motif, has been inv...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2016.01.011

    authors： Gach K,Grądzka I,Wasyk I,Męczyńska-Wielgosz S,Iwaneńko T,Szymański J,Koszuk J,Janecki T,Kruszewski M,Janecka A

    更新日期：2016-03-25 00:00:00

  • Copper modifies liver microsomal UDP-glucuronyltransferase activity through different and opposite mechanisms.

    abstract：:Treatment of hepatic microsomes with Fe(3+)/ascorbate activates UDP-glucuronyltransferase (UGT), a phenomenon totally prevented and reversed by reducing agents. At microM concentrations, iron and copper ions catalyze the formation of ROS through Fenton and/or Haber-Weiss reactions. Unlike iron ions, indiscriminate bin...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2006.12.010

    authors： Letelier ME,Lagos F,Faúndez M,Miranda D,Montoya M,Aracena-Parks P,González-Lira V

    更新日期：2007-04-05 00:00:00

  • Benzene, the exposome and future investigations of leukemia etiology.

    abstract：:Benzene exposure is associated with acute myeloid leukemia (AML), myelodysplastic syndromes (MDS), and probably lymphoma and childhood leukemia. Biological plausibility for a causal role of benzene in these diseases comes from its toxicity to hematopoietic stem cells (HSC) or progenitor cells, from which all leukemias...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2011.02.010

    authors： Smith MT,Zhang L,McHale CM,Skibola CF,Rappaport SM

    更新日期：2011-06-30 00:00:00

  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin suppress AChE activity in NGF treated PC12 cells.

    abstract：:PC12 is a well studied cell model for neuronal differentiation. AChE is also considered as a marker for neuronal differentiation. In this study, we detected the change of AChE activity during the NGF induced differentiation of PC 12 cells, and targeted on the ratio of the activity of AChE on the cell surface, and foun...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2016.08.003

    authors： Xu L,Chen Y,Xie HQH,Xu T,Fu H,Zhang S,Tsim KWK,Bi CWC,Zhao B

    更新日期：2016-11-25 00:00:00

  • Modulatory effects of new curcumin analogues on gamma-irradiation - Induced nephrotoxicity in rats.

    abstract：:In the present study, a new series of 2-amino-pyran-3-carbonitrile derivatives of curcumin 2-7 have been synthesized via one-pot simple and efficient protocol, involving the reaction of curcumin 1 with substituted-benzylidene-malononitrile to modify the 1,3-diketone moiety. The structures of the synthesized compounds ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2016.11.010

    authors： Ismail AF,Zaher NH,El-Hossary EM,El-Gazzar MG

    更新日期：2016-12-25 00:00:00

  • Punarnavine, an alkaloid from Boerhaavia diffusa exhibits anti-angiogenic activity via downregulation of VEGF in vitro and in vivo.

    abstract：:Punarnavine, a quinolizidine alkaloid isolated from Boerhaavia diffusa is known to possess analgesic, anti-inflammatory, hepato-protective, immunomodulatory and anti-proliferative properties. However, its roles in tumor angiogenesis and the involved molecular mechanism are still unknown. Therefore, we examined its ant...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2013.09.007

    authors： Saraswati S,Alhaider AA,Agrawal SS

    更新日期：2013-11-25 00:00:00

  • Effect of several analogs of 2,4,6-triphenyldioxane-1,3 on constitutive androstane receptor activation.

    abstract：:2,4,6-Triphenyldioxane-1,3 (TPD) is a highly effective species-specific inducer of CYP2В in rats. Several analogs of TPD were synthesized to verify a hypothesis that minor changes in the inducer structure can cause changes in induction abilities (R=H, cisTPD and transTPD; R=N(CH(3))(2), transpDMA; R=NO(2), transpNO(2)...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2011.03.005

    authors： Pustylnyak V,Yarushkin A,Kachaylo E,Slynko N,Lyakhovich V,Gulyaeva L

    更新日期：2011-07-15 00:00:00

  • Combined effect of vanadium(V) and chromium(III) on lipid peroxidation in liver and kidney of rats.

    abstract：:Since chromium(III) was demonstrated to have antioxidative action, we have decided to study the effect of this element on V-induced LPO in liver and kidney of rats. Outbred 2-month-old, albino male Wistar rats received daily, for a period of 12 weeks: group I (control), deionized water to drink; group II, sodium metav...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2005.11.008

    authors： Scibior A,Zaporowska H,Ostrowski J,Banach A

    更新日期：2006-02-25 00:00:00

  • Osthole induces cell cycle arrest and apoptosis in head and neck squamous cell carcinoma by suppressing the PI3K/AKT signaling pathway.

    abstract：BACKGROUND:Head and neck squamous cell carcinoma (HNSCC) is one of the most common lethal tumors with a high recurrence rate and low survival rate. Therefore, an urgent need exists for novel and effective treatment strategies for HNSCC patients. METHODS:Osthole, a natural ingredient extracted from Cnidium monnieri (L....

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2019.108934

    authors： Yang J,Zhu XJ,Jin MZ,Cao ZW,Ren YY,Gu ZW

    更新日期：2020-01-25 00:00:00

  • DNA-protein cross-linking by chromium salts.

    abstract：:DNA-protein cross-links were detected in several types of mammalian cells in culture when they were exposed to chromate salts. The cell types included human bronchial epithelial cells--the apparent cell type of origin of the malignancies reported in chromate workers. The level of cross-linking was proportional to the ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(81)90077-6

    authors： Fornace AJ Jr,Seres DS,Lechner JF,Harris CC

    更新日期：1981-09-01 00:00:00

  • Protective role of zinc in nickel induced hepatotoxicity in rats.

    abstract：:This study was planned to determine the protective role of zinc, if any, in attenuating the toxicity induced by nickel sulfate in rat liver. Female Sprague Dawley (SD) rats received either nickel alone in the dose of 800 mg/l in drinking water, zinc alone in the dose of 227 mg/l in drinking water, and nickel plus zinc...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2004.09.012

    authors： Sidhu P,Garg ML,Dhawan DK

    更新日期：2004-11-20 00:00:00

  • Biological and toxicological consequences of quinone methide formation.

    abstract：:Quinone methides are a class of reactive, electrophilic compounds which are capable of alkylating cellular macromolecules. They are formed during xenobiotic biotransformation reactions and are hypothesized to mediate the toxicity of a large number of quinone antitumor drugs as well as several alkylphenols. In addition...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章,评审

    doi：10.1016/0009-2797(93)90117-h

    authors： Thompson DC,Thompson JA,Sugumaran M,Moldéus P

    更新日期：1993-02-01 00:00:00

  • Inhibitory effect of luteolin on hepatocyte growth factor/scatter factor-induced HepG2 cell invasion involving both MAPK/ERKs and PI3K-Akt pathways.

    abstract：:Hepatocyte growth factor (HGF), also known as scatter factor (SF), and its receptor, the c-Met tyrosine kinase, play roles in cancer invasion and metastasis in a wide variety of tumor cells. Clinical observations suggest that HGF can promote metastasis of hepatoma cells while stimulating tumor invasiveness. We use HGF...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2006.01.002

    authors： Lee WJ,Wu LF,Chen WK,Wang CJ,Tseng TH

    更新日期：2006-03-25 00:00:00

  • Toxicity of Trp-P-2 to cultured human and rat keratinocytes.

    abstract：:Keratinocytes cultured from human and rat epidermis exhibited strongly divergent sensitivities to toxicity from the heterocyclic amine food mutagen Trp-P-2. To find a biochemical basis for this difference, the cultured cells were compared in their expression of phase 1 and 2 biotransformation activities, mutagenic act...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/s0009-2797(00)00182-4

    authors： Chun HS,Kuzmicky PA,Kado NY,Rice RH

    更新日期：2000-07-14 00:00:00

  • Differential inhibition of hepatic microsomal alkoxyresorufin O-dealkylation activities by tetrachlorobiphenyls.

    abstract：:Polychlorinated biphenyls (PCBs) elicit a spectrum of biochemical and toxic effects in exposed animals. In the present study, we assessed the effect of PCB structure, using four symmetrically-substituted PCBs, on cytochrome P450 (CYP)-mediated methoxy-, ethoxy- and benzyloxyresorufin O-dealkylase (MROD, EROD and BROD,...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2007.05.004

    authors： Edwards PR,Hrycay EG,Bandiera SM

    更新日期：2007-08-15 00:00:00

  • Studies on rat hepatic hydroxysteroid sulfotransferase--immunochemistry, development and pI variants.

    abstract：:Rat hepatic hydroxysteroid sulfotransferase with sulfoconjugates androsterone (androsterone-sulfating sulfotransferase) is an oligomer consisting of several subunits with distinct pI values but with the same molecular mass (pI variants). N-terminal amino acid sequences of the pI variants are all identical. The enzyme ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(94)90049-3

    authors： Homma H,Nakagome I,Kamakura M,Hirota M,Takahashi M,Matsui M

    更新日期：1994-06-01 00:00:00

  • Chaetocin inhibits IBMX-induced melanogenesis in B16F10 mouse melanoma cells through activation of ERK.

    abstract：:Chaetocin is a natural product isolated from Chaetomium species that has anti-bacterial and anti-myeloma activities. In this study, we investigated the inhibitory effect of chaetocin on melanogenesis and the underlying mechanisms in B16F10 mouse melanoma cells. In the present study, chaetocin significantly inhibited I...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2015.12.021

    authors： Bae JS,Han M,Yao C,Chung JH

    更新日期：2016-02-05 00:00:00

  • Catalysis of nitro-aci tautomerism of the genotoxicant 2-nitropropane by cytosol from rodent and human liver.

    abstract：:2-Nitropropane (2-NP) is a genotoxicant and hepatocarcinogen in rodents. Conversion to propane 2-nitronate (P2N), the anion of the tautomeric aci form of 2-NP, seems to be a pivotal part of the mechanism by which 2-NP causes its toxicity. We tested the hypothesis that the tautomeric equilibrium is influenced by enzyme...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(95)03671-7

    authors： Kohl C,Gescher A

    更新日期：1996-01-05 00:00:00

  • Correlation between the substrate structure and the rate of acetylcholinesterase hydrolysis modeled with the combined quantum mechanical/molecular mechanical studies.

    abstract：:The combined quantum mechanical-molecular mechanical (QM/MM) based computational scheme for modeling the structure-reaction rate correlations was elaborated for the hydrolysis of the set of neutral esters in the active site of acetylcholinesterase (AChE). The energy barriers of hydrolysis were estimated on the basis o...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2010.04.005

    authors： Lushchekina SV,Nemukhin AV,Morozov DI,Varfolomeev SD

    更新日期：2010-09-06 00:00:00

  • The expression of cholinesterases in human renal tumours varies according to their histological types.

    abstract：:The change in the expression of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities in neoplastic colon and lung prompted us to study the possible effect of cancer on the expression of cholinesterases (ChEs) in kidney. Samples of papillary renal cell carcinoma (pRCC), conventional RCC (cRCC), chro...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2008.04.003

    authors： Muñoz-Delgado E,Montenegro MF,Morote-García JC,Campoy FJ,Cabezas-Herrera J,Kovacs G,Vidal CJ

    更新日期：2008-09-25 00:00:00

  • DNA modification induced by 6-mercaptopurine riboside in murine embryos.

    abstract：:The cytostatic drug 6-mercaptopurine riboside (6-MPr) was investigated in mice in order to test the hypothesis that the teratogenicity of this antimetabolite is paralleled by an incorporation into the DNA of the embryos during organogenesis. DNA modification in the embryos was analysed 4 h following s.c. administratio...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(94)90086-8

    authors： Platzek T,Schwabe R,Rahm U,Bochert G

    更新日期：1994-10-01 00:00:00

  • Cytochrome P-450 distribution in rat liver and the effect of sodium phenobarbitone administration.

    abstract：:A microspectrophotometric method for assaying cytochrome P-450 in fresh 24 micrometer unfixed cryostat sections of rat liver has been developed. When used to assay this cytochrome in sections of microsomal preparations it has yielded results equivalent to those obtained by the conventional spectrophotometric assay of ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(78)90108-4

    authors： Gooding PE,Chayen J,Sawyer B,Slater TF

    更新日期：1978-03-01 00:00:00

  • Cromolyn chitosan nanoparticles as a novel protective approach for colorectal cancer.

    abstract：:Colorectal cancer is the third most common cancer in the world. Cromolyn is a mast cell stabilizer and was proposed as an anticancer agent; however its high polarity limits its bioavailability by rapid washing from the body. We formulated 10 cromolyn chitosan nanoparticles (CCSNPs)1 following ionic gelation technique ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2017.07.013

    authors： Motawi TK,El-Maraghy SA,ElMeshad AN,Nady OM,Hammam OA

    更新日期：2017-09-25 00:00:00

  • A new post-intoxication treatment of paraoxon and parathion poisonings using an evolved PON1 variant and recombinant GOT1.

    abstract：:Organophosphate (OP) based pesticides are highly toxic compounds that are still widely used in agriculture around the world. According to World Health Organization (WHO) data, it is estimated that between 250,000 and 370,000 deaths occur yearly around the globe as a result of acute intoxications by pesticides. Current...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2016.05.034

    authors： Goldsmith M,Ashani Y,Margalit R,Nyska A,Mirelman D,Tawfik DS

    更新日期：2016-11-25 00:00:00

  • Human serum paraoxonase (PON1): identification of essential amino acid residues by group-selective labelling and site-directed mutagenesis.

    abstract：:Human serum paraoxonase/arylesterase (PON1, EC 3.1.8.1.) is a calcium-dependent enzyme which hydrolyzes a wide variety of organophosphates, including paraoxon, DFP, sarin and soman. Although the 3-D structure of PON has not yet been determined and its sequence shows no similarity with any other crystallized proteins, ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/s0009-2797(99)00015-0

    authors： Josse D,Xie W,Masson P,Lockridge O

    更新日期：1999-05-14 00:00:00

  • Gastroprotective activity of alkaloid extract and 2-phenylquinoline obtained from the bark of Galipea longiflora Krause (Rutaceae).

    abstract：:As part of our continuing search for bioactive natural products from plants, the present study was carried out in order to evaluate the gastroprotective properties of alkaloid extract and 2-phenylquinoline obtained from the bark of Galipea longiflora (Rutaceae). Anti-ulcer assays were performed using the following pro...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2009.04.001

    authors： Zanatta F,Gandolfi RB,Lemos M,Ticona JC,Gimenez A,Clasen BK,Cechinel Filho V,de Andrade SF

    更新日期：2009-07-15 00:00:00

  • Oroxylin A prevents alcohol-induced hepatic steatosis through inhibition of hypoxia inducible factor 1alpha.

    abstract：:Accumulating data reveal that oroxylin A has beneficial effects against chronic liver disease. The previously studies showed oroxylin A, a flavonoid extracted from Scutellariae radix, improved acute liver injury and accelerated liver regeneration in vivo. However, it's unclear that the effect of oroxylin A on alcoholi...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2018.02.025

    authors： Jin H,Lian N,Bian M,Zhang C,Chen X,Shao J,Wu L,Chen A,Guo Q,Zhang F,Zheng S

    更新日期：2018-04-01 00:00:00

  • Influence of organophosphate poisoning on human dendritic cells.

    abstract：:Organophosphourus compounds (OPC, including nerve agents and pesticides) exhibit acute toxicity by inhibition of acetylcholinesterase. Lung affections are frequent complications and a risk factor for death. In addition, epidemiological studies reported immunological alterations after OPC exposure. In our experiments w...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2013.08.011

    authors： Schäfer M,Koppe F,Stenger B,Brochhausen C,Schmidt A,Steinritz D,Thiermann H,Kirkpatrick CJ,Pohl C

    更新日期：2013-12-05 00:00:00

  • Expression of disodium cromoglygate 'protective' effects observed during V79 cell proliferation.

    abstract：:Disodium cromoglycate (DSCG) in equimolar concentration of 0.56 mumol/l preincubated with an asynchronous cell population decreases the inhibition of proliferation and results in a 100% elimination of inhibition of colony formation induced by benfluron (BF). DSCG protects the cells from unbalanced growth and unbalance...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(93)90002-g

    authors： Horáková K,Vlcková A,Lukácová D

    更新日期：1993-12-01 00:00:00

  • Medium-chain fatty acids and glutathione derivatives as inhibitors of S-nitrosoglutathione reduction mediated by alcohol dehydrogenase 3.

    abstract：:Alcohol dehydrogenase 3 (ADH3) has emerged as an important regulator of protein S-nitrosation in its function as S-nitrosoglutathione (GSNO) reductase. GSNO depletion is associated with various disease conditions, emphasizing the potential value of a specific ADH3 inhibitor. The present study investigated inhibition o...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2009.01.008

    authors： Staab CA,Hellgren M,Grafström RC,Höög JO

    更新日期：2009-06-15 00:00:00