Abstract:
AIM:To propose a quantitative method to detect heparanase-2 (HPA2) and syndecan-1 (Syn-1) using immunohistochemistry in colorectal (colon and rectal) carcinomas compared with nonneoplastic tissues and evaluate the possible role of these molecules in tumor development and extracellular remodeling. METHODS:Cytoplasmic staining of HPA2 and Syn-1 was obtained by standard immunohistochemical reactions in 50 colorectal carcinoma and 20 nonneoplastic large bowels tissues. An image system was used to quantify the immunoexpression by digital computer-assisted method (Matos et al. 2006). The cutoff point for the immunohistochemistry variable was defined by sensibility and specificity curves. Statistical analysis was performed using SPSS version 13.0. RESULTS:HPA2 was over-expressed in colorectal cancer (131.1+/-24.9 o.u./microm) when compared with nonneoplastic tissues (27.9+/-12.2 o.u./microm) (P<0.0001). However, an opposite correlation was observed between Syn-1 and tumor presence, where colorectal tissues expressed lower Syn-1 proteoglycan compared with nonneoplastic tissues, respectively (39.2+/-17.8 o.u./microm) and (102.2+/-25.2 o.u./microm) (P<0.0001). CONCLUSION:A methodology with high sensitivity and specificity is proposed with a cutoff value for HPA2 and Syn-1 in the immunohistochemistry assay to define the presence of tumor. It was demonstrated for the first time in the literature that HPA2 is over-expressed in colorectal carcinoma tissues compared with nonneoplastic tissues. HPA2 over-expression could be possibly related to Syn-1 shedding despite the fact that HPA2 does not present enzymatic activity as HPA1.
journal_name
Eur J Gastroenterol Hepatoljournal_title
European journal of gastroenterology & hepatologyauthors
Peretti T,Waisberg J,Mader AM,de Matos LL,da Costa RB,Conceição GM,Lopes AC,Nader HB,Pinhal MAdoi
10.1097/MEG.0b013e3282fc2649subject
Has Abstractpub_date
2008-08-01 00:00:00pages
756-65issue
8eissn
0954-691Xissn
1473-5687pii
00042737-200808000-00008journal_volume
20pub_type
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journal_title:European journal of gastroenterology & hepatology
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doi:10.1097/00042737-200601000-00020
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
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doi:10.1097/00042737-200303000-00008
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journal_title:European journal of gastroenterology & hepatology
pub_type: 评论,杂志文章
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
pub_type: 杂志文章,评审
doi:10.1097/MEG.0b013e3283474add
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journal_title:European journal of gastroenterology & hepatology
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journal_title:European journal of gastroenterology & hepatology
pub_type: 杂志文章
doi:10.1097/00042737-199905000-00014
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journal_title:European journal of gastroenterology & hepatology
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更新日期:2003-04-01 00:00:00
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journal_title:European journal of gastroenterology & hepatology
pub_type: 杂志文章
doi:10.1097/00042737-200504000-00001
更新日期:2005-04-01 00:00:00
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pub_type: 杂志文章
doi:
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pub_type: 杂志文章
doi:10.1097/00042737-200411000-00027
更新日期:2004-11-01 00:00:00
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journal_title:European journal of gastroenterology & hepatology
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更新日期:2012-03-01 00:00:00