Abstract：
:Identifying parasitism genes encoding proteins secreted from nematodes is the key to understanding the molecular basis of nematode parasitism to insects. In this paper, a cDNA with two introns and three exons encoding a cysteine protease inhibitor was identified by screening a cDNA subtractive library constructed from the nematode, Steinernema carpocapsae, induced by Galleria mellonella hemolymph. The full-length cDNA contains an open reading frame encoding a 139-amino acid protein, designated Sc-cys, with a 19-residue signal peptide. The mature protein was predicted to have a molecular weight of 12,531.59 Da, a pI of 9.44, one disulfide bond, and three conserved domains believed to be important for the inhibition of cysteine proteases. In Basic Local Alignment and Search Tool analyses, the putative protein precursor displayed 26-42% identities to a multitude of cystatins or cystatin-like proteins. Phylogenetic analysis suggested the novel cystatin is likely a new member of the family 2 cystatins. Reverse northern blot, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), and real-time RT-PCR analyses showed that the expression level of Sc-cys was upregulated substantially after induction by insect hemolymph. The specific analysis of genes encoding secretory proteins is providing a profile of putative parasitism genes expressed in S. carpocapsae throughout the parasitic cycle.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Hao YJ,Montiel R,Nascimento G,Toubarro D,Simoes Ndoi
10.1007/s00436-008-1030-4subject
Has Abstractpub_date
2008-08-01 00:00:00pages
671-83issue
3eissn
0932-0113issn
1432-1955journal_volume
103pub_type
杂志文章abstract：:Four new and four previously described species of Annulotrema were collected from the gills of four species (three genera, i.e. Alestes, Hydrocynus and Brycinus) of African tetras from Lake Turkana, Kenya: Annulotrema alestesnursi Paperna, 1973 from Brycinus nurse; Annulotrema ansatum n. sp., Annulotrema besalis Řehul...
journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-015-4682-x
更新日期：2015-11-01 00:00:00
abstract：:Physaloptera rara (Spirurida: Physalopteridae) has been found in dogs, coyotes, raccoons, wolves, foxes, cats, and bobcats in North America. The parasites' developmental cycles involve insects, including beetles, cockroaches, and crickets, as intermediate hosts. The nematodes firmly attach to the wall of the stomach a...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-013-3526-9
更新日期：2013-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050681
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pub_type： 杂志文章,评审
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00931082
更新日期：1990-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,评审
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/BF00930829
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1998-05-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-012-2919-5
更新日期：2012-10-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s00436-008-1175-1
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journal_title：Parasitology research
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doi：10.1007/s00436-012-2901-2
更新日期：2012-08-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Parasitology research
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更新日期：2003-02-01 00:00:00
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更新日期：2008-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2008-09-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
doi：10.1007/s004360050384
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2009-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：2014-10-01 00:00:00
abstract：:The present work aimed at studying the possible association of HLA antigens with Entamoeba histolytica/E. dispar asymptomatic infection in a Mexican mestizo population. A case-control design was selected for evaluation of the role of genetic markers in parasite infection. For this purpose the HLA-A, HLA-B, and HLA-DR ...
journal_title：Parasitology research
pub_type： 杂志文章
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更新日期：1999-10-01 00:00:00
