Abstract:
:Tumor necrosis factor-alpha (TNF-alpha) has been shown to induce the differentiation of CD34(+) cells toward dendritic cells (DCs). We have previously shown that DCs are co-generated from human CD34(+) cells during erythroid or megakaryocytic differentiation in the presence of TNF-alpha, and those DCs are able to stimulate autologous T cell proliferation. The aim of this study was to learn whether the co-stimulation of granulocyte colony-stimulating factor (G-CSF) and TNF-alpha would generate neutrophil progenitors and DCs together from human CD34(+) cells, and if this was the case, to clarify the phenotypic and functional characteristics of these DCs. When highly purified human CD34(+) cells were cultured for 7 days with G-CSF alone, the generated cells predominantly expressed a granulocyte marker, CD15, and then differentiated into neutrophils after 14 days of culture. The addition of TNF-alpha with G-CSF markedly decreased the number of CD15(+) cells without affecting the total number of cells during 7 days of culture. Almost one third of the generated cells were positive for CD11c and CD123. Furthermore, CD11c(+) cells were found to phagocytose CD15(+) cells and were able to induce allogeneic, but not autologous, T cell proliferation in the mixed lymphocyte reaction (MLR). On the other hand, the CD11c(+) cells generated by TNF-alpha and cytokines capable of inducing erythroid differentiation were able to stimulate autologous T cells. There was a difference in the expression of CD80, CD83 and CD86 among CD11c(+) cells induced by G-CSF plus TNF-alpha and those generated by interleukin-3, stem cell factor, and erythropoietin plus TNF-alpha. These results indicate that the co-stimulation of human CD34(+) cells with G-CSF and TNF-alpha induces the phagocytosis of co-developing neutrophil progenitors by DCs, and the stimulatory effects of these DCs on autologous T cells is different from that of DCs generated from CD34(+) cells during erythroid differentiation.
journal_name
Int J Hematoljournal_title
International journal of hematologyauthors
Saito Y,Guo YM,Hirokawa M,Saito K,Komatsuda A,Takahashi N,Fujishima M,Fujishima N,Yamashita J,Sawada Kdoi
10.1007/s12185-008-0098-zsubject
Has Abstractpub_date
2008-07-01 00:00:00pages
64-72issue
1eissn
0925-5710issn
1865-3774pii
10.1007/s12185-008-0098-zjournal_volume
88pub_type
杂志文章abstract::Thrombomodulin (TM) is an endothelial cell membrane glycoprotein which modulates coagulation via the formation of thrombin-TM complexes. We investigated the human megakaryoblastic cell line (UT-7) for the presence of functional TM on the cell surface and in cell lysates using a specific enzyme-linked immunosorbent ass...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::Interferon regulatory factor-8 (IRF8) is a transcription factor expressed in hematopoietic cells, particularly in mononuclear phagocytes [monocytes/macrophages and dendritic cells (DCs)] and their progenitors. Various studies have demonstrated that IRF8 is essential for the development of monocytes, DCs, eosinophils, ...
journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1007/s12185-015-1761-9
更新日期:2015-04-01 00:00:00
abstract::African Burkitt lymphomas (BL) are highly aggressive lymphomas mainly affecting children and young adults in Africa. This lymphoma was marked by its high sensitivity to chemotherapy in comparison to Sporadic Burkitt lymphoma. In this study, we evaluated the treatment response and survival of patients with CMA protocol...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-010-0591-z
更新日期:2010-06-01 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) shows a remarkable heterogeneity, with some patients having an almost normal lifespan, others surviving only several months after diagnosis despite intensive therapy. The aim of this study was to investigate the serum thymidine kinase 1 (TK1) concentration in Chinese patients with CL...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-009-0380-8
更新日期:2009-09-01 00:00:00
abstract::Umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages. Because mesenchymal stem cells (MSCs) from bone marrow have been regarded as good materials for cell/gene therapy as well as for tissue engineering because of their multidifferentiation poten...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1532/ijh97.a10404
更新日期:2005-02-01 00:00:00
abstract::Rapid vitamin K antagonist (VKA) reversal is required in patients experiencing major bleeding or requiring urgent surgery. Four-factor prothrombin complex concentrate (4F-PCC; Beriplex®/Kcentra®) was shown in two large randomized controlled, international phase 3b trials to be an effective alternative to plasma for ur...
journal_title:International journal of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s12185-017-2311-4
更新日期:2017-12-01 00:00:00
abstract::An inherited antithrombin deficiency is an autosomal dominant thrombotic disorder. We identified two pedigrees of inherited type I antithrombin deficiency and two responsible mutations in each. A novel 21-22delAA appeared to have caused a frameshift with a premature termination at amino acid +63 in one patient and a l...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-010-0763-x
更新日期:2011-02-01 00:00:00
abstract::The prognostic significance of Bcl-2, Bcl-6, p53, topoisomerase II, and β-tubulin expression was evaluated in diffuse large B-cell lymphoma (DLBCL) patients treated with cyclophosphamide, doxorubicin, vincristine, prednisolone, and rituximab. Eight-year progression-free survival (PFS, P = 0.006) and overall survival (...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-015-1911-0
更新日期:2016-02-01 00:00:00
abstract::A multiple myeloma (MM) cell line, MSG1, which depends on HS23 stromal cells for its survival, was established from the pleural effusion of a patient with MM who expressed the M-protein of IgA-λ in his serum. During the first 2 months of culture, the myeloma cells survived on adhesive cells from the pleural effusion a...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-010-0698-2
更新日期:2010-11-01 00:00:00
abstract::We established two novel cell lines, designated as IMS-BC1 and IMS-BC2, from two patients with chronic myelogenous leukemia in blast crisis. The two cell lines were positive for CD13 and CD33 and negative for CD34 and HLA-DR by surface marker analysis. IMS-BC1 had four Philadelphia (Ph1) chromosomes and a breakpoint w...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1016/s0925-5710(98)00007-3
更新日期:1998-04-01 00:00:00
abstract::We describe a variant form, French-American-British (FAB) M3v, of acute promyelocytic leukemia (APL; FAB M3) with atypical morphocytochemical features, immature antigens (CD34 and HLA-DR) and marked myelofibrosis (MF). Usual APL cells do not express CD34 or HLA-DR antigens. MF may be more frequently observed in patien...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/BF02982068
更新日期:2001-10-01 00:00:00
abstract::Numerous studies have demonstrated that microRNA-21 (miR-21), as an oncogene, is involved in the occurrence of many types of tumor and the sensitivity of tumor cells to chemotherapeutic drugs. In the present study, we investigated whether miR-21 is involved in regulating the sensitivity of the diffuse large B-cell lym...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-012-1256-x
更新日期:2013-02-01 00:00:00
abstract::Posttransplant lymphoproliferative disorder (PTLD) after allogeneic hematopoietic cell transplantation (HCT) is usually donor derived, associated with Epstein-Barr virus (EBV), and of B-cell origin. T-cell PTLD (T-PTLD) after allogeneic HCT is extremely rare. Four of 1015 (0.39%) allogeneic HCT patients were diagnosed...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-020-02890-1
更新日期:2020-08-01 00:00:00
abstract::Autologous stem cell transplantation is considered the best post-induction therapy for multiple myeloma (MM). Therefore, therapy for myeloma should be chosen not only on the basis of efficacy, but also taking into account their impact on the hematopoietic stem cell compartment. We describe the case of a MM patient in ...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-009-0354-x
更新日期:2009-07-01 00:00:00
abstract::We retrospectively reviewed clinical and hematologic features of nine patients with acquired idiopathic sideroblastic anemia (AISA). Seven of them had ringed sideroblasts (RS) more than 15% of marrow nucleated cells. RS persisted in the marrow even in the remaining two patients who had a relatively low marrow erythrob...
journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
doi:10.1016/s0925-5710(97)00112-6
更新日期:1998-02-01 00:00:00
abstract::Plasma cell leukemia (PCL) is a highly aggressive plasma cell disease characterized by a poor prognosis and a low response rate to conventional therapy. Herein, we describe a 69-year-old patient with primary PCL, developing testicular disease while in complete hematological remission, following by muscle involvement a...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-010-0745-z
更新日期:2011-02-01 00:00:00
abstract::We statistically analyzed the hematologic findings of patients with refractory anemia (RA) to identify parameters associated with a poor prognosis. We first separated the RA patients into two groups: one group with disease progression and one without. The patients with disease progression were predominantly male and h...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Novel approaches to the treatment of haemophilia are needed due to the limitations of the current standard of care, factor replacement therapy. Aspirations include lessening the treatment burden and effectively preventing joint damage. Treating haemophilia by restoring thrombin generation may be an effective approach....
journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1007/s12185-018-2548-6
更新日期:2020-01-01 00:00:00
abstract::The unique feature of paroxysmal nocturnal hemoglobinuria (PNH), a chronic disease with severe hemolytic anemia, is the presence of a population of blood cells that, being deficient in surface proteins tethered to the membrane through a glycosylphosphatidylinositol molecule, are said to have the PNH phenotype. Therefo...
journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1532/IJH97.06117
更新日期:2006-08-01 00:00:00
abstract::We report a case of successful umbilical cord blood transplantation (CBT) for Epstein-Barr virus (EBV)-associated lymphoproliferative disease (LPD) in a 6-year-old girl. The patient had hemophagocytic syndrome with excessive circulating levels of EBV DNA that was refractory to immunochemotherapy. Multiple hepatospleni...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1532/ijh97.04081
更新日期:2004-12-01 00:00:00
abstract::Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is now undergoing clinical trials. We investigated the effects of rhG-CSF on the function of neutrophils in vivo in healthy volunteers. rhG-CSF (0.5 micrograms/kg) was injected subcutaneously for 6 consecutive days. The number of neutrophils in peripher...
journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Philadelphia (Ph) chromosome as a result of t (9; 22) (q34; q11) is observed in more than 90% of chromic myeloid leukemia (CML) patients. Cases in which the typical Ph chromosome is not visible at the karyotype level comprise 5-10% of CML patients. CML cases with fusion transcripts such as e13a3 in which ABL exon 3 ra...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-009-0368-4
更新日期:2009-09-01 00:00:00
abstract::Lymphomas are a heterogeneous group of disease entities with well-defined clinical, morphological, immunophenotypic, and cytogenetic characteristics. Moreover, regional and racial differences have been reported in their incidence and subtype compositions. Here, we reviewed the epidemiology of lymphomas and summarized ...
journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1007/s12185-018-2403-9
更新日期:2018-04-01 00:00:00
abstract::Immunoglobulin light chain (AL) amyloidosis is a systemic disorder caused by depositions of insoluble amyloid fibrils that are composed of fragments of monoclonal light chains produced by abnormal plasma cells. The prognosis is reported to be poor; median survival time (MST) is 1-2 years overall, and is 6 months in pa...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-015-1814-0
更新日期:2015-08-01 00:00:00
abstract::The study was aimed to explore the efficacy and safety of allo-HSCT with high-dose cyclophosphamide-induced immune tolerance for SAA. In the present study, 20 cases (12 male, 8 female; average age = 17.8 years) received reduced-intensity conditioning allo-HSCT from August 2012 to August 2014 in the Beijing Military Re...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-016-2106-z
更新日期:2016-12-01 00:00:00
abstract::We describe the case of a patient with peripheral gamma/delta T-cell lymphoma (T-ML) with hepatosplenomegaly, generalized lymphadenopathy, and bone marrow involvement. A 44-year-old man had lymphoma, which became clinically apparent 2 months after the onset of myositis and insulin-dependent diabetes mellitus. A cervic...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::The t(14;19)(q32;q13) is a recurring translocation found in some patients with chronic lymphocytic leukemia (CLL), and the t(14;19) juxtaposes the BCL3 gene on chromosome 19 with the immunoglobulin heavy chain gene (IGH) locus on chromosome 14. Genomic DNAs from 49 patients with chronic B-cell leukemia and the related...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::In vitro treatment of bone marrow cells to expand stem cells may lead to impaired hematopoietic long-term reconstitution. Here we report on studies that show that cytokine-treated cells maintain short-term reconstitution, but lose that potential with time posttransplantation. Hematopoietic progenitors assayed in vitro...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Integrins, a family of noncovalently associated alpha beta heterodimeric adhesion receptors, are involved in a variety of pathological and physiological processes. The importance of integrins is evident in the severe pathogenic consequences of their congenital deficiencies: Glanzmann thrombasthenia (GT) and leukocyte ...
journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:
更新日期:2000-12-01 00:00:00
abstract::Mesenchymal stromal cells may reverse acute inflammatory disorders. The placenta is important in feto-maternal tolerance. We have used placenta-derived decidua stromal cells (DSCs) to treat graft-versus-host disease and found an immunomodulatory and anti-inflammatory effect. We here report the use of DSCs in two patie...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-019-02804-w
更新日期:2020-04-01 00:00:00