Abstract:
:Numerous studies have demonstrated that microRNA-21 (miR-21), as an oncogene, is involved in the occurrence of many types of tumor and the sensitivity of tumor cells to chemotherapeutic drugs. In the present study, we investigated whether miR-21 is involved in regulating the sensitivity of the diffuse large B-cell lymphoma (DLBCL) cell line CRL2631 to the cyclophosphamide, vincristine, Adriamycin, and prednisone (CHOP) chemotherapeutic regimen. Knockdown of miR-21 with antisense oligonucleotides significantly increased the cytotoxic effects of the CHOP regimen in CRL2631 cells. A luciferase reporter assay showed that PTEN is a target gene of miR-21 in CRL2631 cells, and subsequent experiments demonstrated that miR-21 impacts the PI3K/AKT signaling pathway through the regulation of PTEN, thereby affecting cellular sensitivity to the CHOP chemotherapeutic regimen. Furthermore, knockdown of NF-κB decreased miR-21 expression and sensitized CRL2631 cells to CHOP treatment. These results provide evidence that it may be possible to overcome microRNA-based DLBCL drug resistance.
journal_name
Int J Hematoljournal_title
International journal of hematologyauthors
Bai H,Wei J,Deng C,Yang X,Wang C,Xu Rdoi
10.1007/s12185-012-1256-xsubject
Has Abstractpub_date
2013-02-01 00:00:00pages
223-31issue
2eissn
0925-5710issn
1865-3774journal_volume
97pub_type
杂志文章abstract::Gene therapies are classified into two major categories, namely, in vivo and ex vivo. Clinical trials of human gene therapy began with the ex vivo techniques. Based on the initial successes of gene-therapy clinical trials, these approaches have spread worldwide. The number of gene therapy trials approved worldwide inc...
journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1007/s12185-016-2030-2
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abstract::Spontaneous improvement (SI) occurs more frequently in children with chronic immune thrombocytopenia (cITP) than in adults. It is generally accepted that, with the exception of splenectomy, conventional medical approaches for cITP do not change the natural course of the disease. Previous studies on pediatric cITP have...
journal_title:International journal of hematology
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journal_title:International journal of hematology
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abstract::A 32-year-old male patient with severe factor VIII (FVIII) deficiency had developed a high-titer FVIII inhibitor at age 13. Recurrent hemarthroses caused bony destruction in both knees, significantly impairing his ability to walk. Knee examination revealed 20 degrees of varus, destruction of the medial joint line, and...
journal_title:International journal of hematology
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abstract::We describe two patients with primary cardiac malignant lymphoma involving the right atrium and superior vena cava, resulting in intractable right cardiac failure and superior vena cava syndrome. Patients were diagnosed by surgical myocardial biopsy and were treated with combination chemotherapy for non-Hodgkin's lymp...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
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abstract::We report a case of HIV-negative Burkitt lymphoma (BL) that relapsed 9 years after complete remission. We performed a polymerase chain reaction analysis of three regions of the VDJ junction of the immunoglobulin heavy chain (IGH) gene and compared the clonality of the first and second BL lesions, which were found to b...
journal_title:International journal of hematology
pub_type: 杂志文章
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/BF02982088
更新日期:2001-12-01 00:00:00
abstract::A new complicated Ph1 translocation involving five chromosomes, t(9;22;21;11;inv ins(12)-(q15p12p13))(q34;q11;q22;q13;q15), was found in a 64-year-old Korean woman with chronic myelocytic leukemia (CML). At presentation, the patient was found to be in the accelerated phase, she entered the chronic phase after six cycl...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
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journal_title:International journal of hematology
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-009-0290-9
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/BF02982068
更新日期:2001-10-01 00:00:00
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journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
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更新日期:1998-12-01 00:00:00
abstract::To examine the effects and optimal dose of danazol on idiopathic thrombocytopenic purpura (ITP), we administered a low-medium dose to 14 patients with this disease. A low-medium dose of danazol was effective in maintaining the platelet count at a high level, even after the dose of prednisolone was reduced. A low-mediu...
journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
doi:
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abstract::The de-novo synthesis and secretion of beta-thromboglobulin (BTG) by a human megakaryoblastic cell line (MEG-01) were studied by measuring and immunoblotting of BTG in culture supernatant and immunoprecipitation of radiolabeled BTG synthesized after incubation with [35S]methionine. It was demonstrated that BTG synthes...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1532/IJH97.06133
更新日期:2006-10-01 00:00:00
abstract::Late-onset hemorrhagic cystitis (LOHC) is a common complication following allogeneic hematopoietic stem cell transplantation (HSCT), but its cause remains obscure. More attention to risk factors for LOHC is needed. We retrospectively analyzed patients with advanced leukemia who had been treated with ATG-containing con...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-013-1350-8
更新日期:2013-07-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-018-2472-9
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journal_title:International journal of hematology
pub_type: 杂志文章
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journal_title:International journal of hematology
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journal_title:International journal of hematology
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journal_title:International journal of hematology
pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
abstract::Aplastic anemia (AA) and pure red cell aplasia (PRCA) appear to be more prevalent in Asian countries including Korea. However, there are no exact data regarding its prevalence and frequency of allogeneic hematopoietic cell transplantation (HCT) in Korea. Here, we present demographic data relating to AA/PRCA/MDS in Kor...
journal_title:International journal of hematology
pub_type: 临床试验,杂志文章,多中心研究
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-009-0440-0
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1007/s12185-012-1137-3
更新日期:2012-08-01 00:00:00
abstract::Chimeric antigen receptor (CAR)-modified T cells have generated broad interest in oncology following a series of dramatic clinical successes in patients with chemorefractory B cell malignancies. CAR therapy now appears to be on the cusp of regulatory approval as a cell-based immunotherapy. We review here the T cell bi...
journal_title:International journal of hematology
pub_type: 杂志文章,评审
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更新日期:2016-07-01 00:00:00
abstract::We retrospectively analyzed 126 acute myelogenous leukemia (AML) patients aged > or =60 years who had all been referred to the same hematological department between 1989 and 1999. In 76 de novo AML cases, 53 patients (median age, 72 years) were treated with combination chemotherapy (CT) for remission induction. Comple...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/BF02981978
更新日期:2002-01-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1007/BF03165100
更新日期:2002-08-01 00:00:00
abstract::The prognostic significance of Bcl-2, Bcl-6, p53, topoisomerase II, and β-tubulin expression was evaluated in diffuse large B-cell lymphoma (DLBCL) patients treated with cyclophosphamide, doxorubicin, vincristine, prednisolone, and rituximab. Eight-year progression-free survival (PFS, P = 0.006) and overall survival (...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-015-1911-0
更新日期:2016-02-01 00:00:00
abstract::Hereditary red blood cell (RBC) membranopathies are characterized by mutations in genes encoding skeletal proteins that alter the membrane complex structure. Hereditary spherocytosis (HS) is the most common inherited RBC membranopathy leading to hereditary hemolytic anemia with a worldwide distribution and an estimate...
journal_title:International journal of hematology
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更新日期:2020-10-19 00:00:00